UniProtKB - Q96ED9 (HOOK2_HUMAN)
Protein Hook homolog 2
HOOK2
Functioni
Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed:32073997).
4 PublicationsGO - Molecular functioni
- dynein light intermediate chain binding Source: GO_Central
- identical protein binding Source: UniProtKB
- microtubule binding Source: GO_Central
GO - Biological processi
- cytoplasmic microtubule organization Source: GO_Central
- cytoskeleton-dependent intracellular transport Source: GO_Central
- early endosome to late endosome transport Source: UniProtKB
- endocytosis Source: ProtInc
- endosome organization Source: UniProtKB
- endosome to lysosome transport Source: UniProtKB
- lysosome organization Source: UniProtKB
- protein localization to perinuclear region of cytoplasm Source: UniProtKB
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q96ED9 |
SignaLinki | Q96ED9 |
SIGNORi | Q96ED9 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Hook homolog 2Short name: h-hook2 Short name: hHK2 |
Gene namesi | Name:HOOK2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19885, HOOK2 |
MIMi | 607824, gene |
neXtProti | NX_Q96ED9 |
VEuPathDBi | HostDB:ENSG00000095066 |
Subcellular locationi
Golgi apparatus
- trans-Golgi network 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- centrosome 1 Publication
- cytoskeleton By similarity
Note: Colocalizes with aggresomes, which are aggregates of misfolded proteins, at the centrosome (PubMed:17540036). Also localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, multivesicular bodies (MVBs), lysosome, or mitochondria (PubMed:17540036, PubMed:32073997). Often found in close association with microtubules (PubMed:17540036). Localizes to the manchette in elongating spermatids (By similarity).By similarity2 Publications
Cytoskeleton
- centrosome Source: UniProtKB
- microtubule Source: UniProtKB-KW
Cytosol
- cytosol Source: HPA
Golgi apparatus
- trans-Golgi network Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- FHF complex Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Golgi apparatus, MicrotubulePathology & Biotechi
Organism-specific databases
DisGeNETi | 29911 |
OpenTargetsi | ENSG00000095066 |
PharmGKBi | PA134986864 |
Miscellaneous databases
Pharosi | Q96ED9, Tbio |
Genetic variation databases
BioMutai | HOOK2 |
DMDMi | 296439323 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219194 | 1 – 719 | Protein Hook homolog 2Add BLAST | 719 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 163 | PhosphoserineCombined sources | 1 | |
Modified residuei | 230 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 710 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q96ED9 |
jPOSTi | Q96ED9 |
MassIVEi | Q96ED9 |
MaxQBi | Q96ED9 |
PaxDbi | Q96ED9 |
PeptideAtlasi | Q96ED9 |
PRIDEi | Q96ED9 |
ProteomicsDBi | 76396 [Q96ED9-1] 76397 [Q96ED9-2] |
PTM databases
iPTMneti | Q96ED9 |
PhosphoSitePlusi | Q96ED9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000095066, Expressed in adenohypophysis and 193 other tissues |
ExpressionAtlasi | Q96ED9, baseline and differential |
Genevisiblei | Q96ED9, HS |
Organism-specific databases
HPAi | ENSG00000095066, Low tissue specificity |
Interactioni
Subunit structurei
Self-associates (PubMed:18799622).
Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FHIP1B, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3 (PubMed:18799622). May interact directly with AKTIP/FTS, HOOK1 and HOOK3 (PubMed:18799622, PubMed:32073997). Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect (PubMed:18799622).
Interacts with CNTRL (PubMed:17140400).
Interacts with microtubules (PubMed:11238449).
Interacts with ZC3H14 (PubMed:19273536).
Interacts with LRGUK (via guanylate kinase-like domain) (By similarity).
Interacts with CCDC181 (By similarity).
Interacts with AP4M1; the interaction is direct, mediates the interaction between FTS-Hook-FHIP (FHF) complex and AP-4 and the perinuclear distribution of AP-4 (PubMed:32073997).
By similarity5 PublicationsBinary interactionsi
Q96ED9
Isoform 2 [Q96ED9-2]
GO - Molecular functioni
- dynein light intermediate chain binding Source: GO_Central
- identical protein binding Source: UniProtKB
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 118957, 179 interactors |
CORUMi | Q96ED9 |
IntActi | Q96ED9, 140 interactors |
MINTi | Q96ED9 |
STRINGi | 9606.ENSP00000380785 |
Miscellaneous databases
RNActi | Q96ED9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 122 | Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 548 | Sufficient for interaction with microtubulesAdd BLAST | 548 | |
Regioni | 1 – 161 | Required for localization to the centrosome and induction of aggresome formationAdd BLAST | 161 | |
Regioni | 533 – 719 | Required for localization to the centrosome and induction of aggresome formationAdd BLAST | 187 | |
Regioni | 584 – 719 | Sufficient for interaction with CNTRL1 PublicationAdd BLAST | 136 | |
Regioni | 696 – 719 | DisorderedSequence analysisAdd BLAST | 24 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 180 – 427 | Sequence analysisAdd BLAST | 248 | |
Coiled coili | 455 – 607 | Sequence analysisAdd BLAST | 153 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QTJ1, Eukaryota |
GeneTreei | ENSGT00940000160152 |
HOGENOMi | CLU_011214_2_0_1 |
InParanoidi | Q96ED9 |
OMAi | PKADSNM |
OrthoDBi | 398210at2759 |
PhylomeDBi | Q96ED9 |
TreeFami | TF320231 |
Family and domain databases
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR008636, Hook_C IPR043936, HOOK_N |
Pfami | View protein in Pfam PF05622, HOOK, 1 hit PF19047, HOOK_N, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVDKAELCG SLLTWLQTFH VPSPCASPQD LSSGLAVAYV LNQIDPSWFN
60 70 80 90 100
EAWLQGISED PGPNWKLKVS NLKMVLRSLV EYSQDVLAHP VSEEHLPDVS
110 120 130 140 150
LIGEFSDPAE LGKLLQLVLG CAISCEKKQD HIQRIMTLEE SVQHVVMEAI
160 170 180 190 200
QELMTKDTPD SLSPETYGNF DSQSRRYYFL SEEAEEGDEL QQRCLDLERQ
210 220 230 240 250
LMLLSEEKQS LAQENAGLRE RMGRPEGEGT PGLTAKKLLL LQSQLEQLQE
260 270 280 290 300
ENFRLESGRE DERLRCAELE REVAELQHRN QALTSLAQEA QALKDEMDEL
310 320 330 340 350
RQSSERAGQL EATLTSCRRR LGELRELRRQ VRQLEERNAG HAERTRQLED
360 370 380 390 400
ELRRAGSLRA QLEAQRRQVQ ELQGQRQEEA MKAEKWLFEC RNLEEKYESV
410 420 430 440 450
TKEKERLLAE RDSLREANEE LRCAQLQPRG LTQADPSLDP TSTPVDNLAA
460 470 480 490 500
EILPAELRET LLRLQLENKR LCRQEAADRE RQEELQRHLE DANRARHGLE
510 520 530 540 550
TQHRLNQQQL SELRAQVEDL QKALQEQGGK TEDAISILLK RKLEEHLQKL
560 570 580 590 600
HEADLELQRK REYIEELEPP TDSSTARRIE ELQHNLQKKD ADLRAMEERY
610 620 630 640 650
RRYVDKARMV MQTMEPKQRP AAGAPPELHS LRTQLRERDV RIRHLEMDFE
660 670 680 690 700
KSRSQREQEE KLLISAWYNM GMALQQRAGE ERAPAHAQSF LAQQRLATNS
710
RRGPLGRLAS LNLRPTDKH
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EMR5 | K7EMR5_HUMAN | Protein Hook homolog 2 | HOOK2 | 157 | Annotation score: | ||
K7ENU5 | K7ENU5_HUMAN | Protein Hook homolog 2 | HOOK2 | 270 | Annotation score: | ||
K7EJ48 | K7EJ48_HUMAN | Protein Hook homolog 2 | HOOK2 | 226 | Annotation score: | ||
K7EMG7 | K7EMG7_HUMAN | Protein Hook homolog 2 | HOOK2 | 127 | Annotation score: | ||
K7EK29 | K7EK29_HUMAN | Protein Hook homolog 2 | HOOK2 | 93 | Annotation score: | ||
A0A7I2V5Y2 | A0A7I2V5Y2_HUMAN | Protein Hook homolog 2 | HOOK2 | 170 | Annotation score: | ||
K7EJ65 | K7EJ65_HUMAN | Protein Hook homolog 2 | HOOK2 | 209 | Annotation score: | ||
K7EJA7 | K7EJA7_HUMAN | Protein Hook homolog 2 | HOOK2 | 71 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 376 | R → L in AAC09299 (PubMed:9927460).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017575 | 10 | G → R. Corresponds to variant dbSNP:rs2305376Ensembl. | 1 | |
Natural variantiVAR_017576 | 488 | H → Q1 PublicationCorresponds to variant dbSNP:rs897804Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009342 | 534 – 535 | Missing in isoform 2. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF044924 mRNA Translation: AAC09299.1 AC018761 Genomic DNA No translation available. BC012443 mRNA Translation: AAH12443.1 |
CCDSi | CCDS42507.1 [Q96ED9-2] CCDS42508.1 [Q96ED9-1] |
RefSeqi | NP_001093646.1, NM_001100176.1 [Q96ED9-2] NP_037444.2, NM_013312.2 [Q96ED9-1] |
Genome annotation databases
Ensembli | ENST00000264827; ENSP00000264827; ENSG00000095066 [Q96ED9-2] ENST00000397668; ENSP00000380785; ENSG00000095066 |
GeneIDi | 29911 |
KEGGi | hsa:29911 |
MANE-Selecti | ENST00000397668.8; ENSP00000380785.2; NM_013312.3; NP_037444.2 |
UCSCi | uc002muy.3, human [Q96ED9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF044924 mRNA Translation: AAC09299.1 AC018761 Genomic DNA No translation available. BC012443 mRNA Translation: AAH12443.1 |
CCDSi | CCDS42507.1 [Q96ED9-2] CCDS42508.1 [Q96ED9-1] |
RefSeqi | NP_001093646.1, NM_001100176.1 [Q96ED9-2] NP_037444.2, NM_013312.2 [Q96ED9-1] |
3D structure databases
SMRi | Q96ED9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 118957, 179 interactors |
CORUMi | Q96ED9 |
IntActi | Q96ED9, 140 interactors |
MINTi | Q96ED9 |
STRINGi | 9606.ENSP00000380785 |
PTM databases
iPTMneti | Q96ED9 |
PhosphoSitePlusi | Q96ED9 |
Genetic variation databases
BioMutai | HOOK2 |
DMDMi | 296439323 |
Proteomic databases
EPDi | Q96ED9 |
jPOSTi | Q96ED9 |
MassIVEi | Q96ED9 |
MaxQBi | Q96ED9 |
PaxDbi | Q96ED9 |
PeptideAtlasi | Q96ED9 |
PRIDEi | Q96ED9 |
ProteomicsDBi | 76396 [Q96ED9-1] 76397 [Q96ED9-2] |
Protocols and materials databases
Antibodypediai | 26165, 112 antibodies from 21 providers |
DNASUi | 29911 |
Genome annotation databases
Ensembli | ENST00000264827; ENSP00000264827; ENSG00000095066 [Q96ED9-2] ENST00000397668; ENSP00000380785; ENSG00000095066 |
GeneIDi | 29911 |
KEGGi | hsa:29911 |
MANE-Selecti | ENST00000397668.8; ENSP00000380785.2; NM_013312.3; NP_037444.2 |
UCSCi | uc002muy.3, human [Q96ED9-1] |
Organism-specific databases
CTDi | 29911 |
DisGeNETi | 29911 |
GeneCardsi | HOOK2 |
HGNCi | HGNC:19885, HOOK2 |
HPAi | ENSG00000095066, Low tissue specificity |
MIMi | 607824, gene |
neXtProti | NX_Q96ED9 |
OpenTargetsi | ENSG00000095066 |
PharmGKBi | PA134986864 |
VEuPathDBi | HostDB:ENSG00000095066 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTJ1, Eukaryota |
GeneTreei | ENSGT00940000160152 |
HOGENOMi | CLU_011214_2_0_1 |
InParanoidi | Q96ED9 |
OMAi | PKADSNM |
OrthoDBi | 398210at2759 |
PhylomeDBi | Q96ED9 |
TreeFami | TF320231 |
Enzyme and pathway databases
PathwayCommonsi | Q96ED9 |
SignaLinki | Q96ED9 |
SIGNORi | Q96ED9 |
Miscellaneous databases
BioGRID-ORCSi | 29911, 13 hits in 1045 CRISPR screens |
ChiTaRSi | HOOK2, human |
GeneWikii | HOOK2 |
GenomeRNAii | 29911 |
Pharosi | Q96ED9, Tbio |
PROi | PR:Q96ED9 |
RNActi | Q96ED9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000095066, Expressed in adenohypophysis and 193 other tissues |
ExpressionAtlasi | Q96ED9, baseline and differential |
Genevisiblei | Q96ED9, HS |
Family and domain databases
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR008636, Hook_C IPR043936, HOOK_N |
Pfami | View protein in Pfam PF05622, HOOK, 1 hit PF19047, HOOK_N, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HOOK2_HUMAN | |
Accessioni | Q96ED9Primary (citable) accession number: Q96ED9 Secondary accession number(s): O60562 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | May 18, 2010 | |
Last modified: | February 23, 2022 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families