UniProtKB - Q96DZ5 (CLIP3_HUMAN)
Protein
CAP-Gly domain-containing linker protein 3
Gene
CLIP3
Organism
Homo sapiens (Human)
Status
Functioni
Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics. May modulate the cellular compartmentalization of AKT kinase family and promote its cell membrane localization, thereby playing a role in glucose transport in adipocytes.1 Publication
Miscellaneous
The N-terminal half is dispensable for proper Golgi targeting, whereas the GoLD region is required.
GO - Molecular functioni
- ganglioside binding Source: UniProtKB
- microtubule binding Source: UniProtKB
- microtubule plus-end binding Source: GO_Central
GO - Biological processi
- chaperone-mediated protein transport Source: UniProtKB
- cytoplasmic microtubule organization Source: GO_Central
- fat cell differentiation Source: UniProtKB
- membrane biogenesis Source: UniProtKB
- negative regulation of microtubule polymerization Source: UniProtKB
- peptidyl-L-cysteine S-palmitoylation Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of endocytosis Source: UniProtKB
- positive regulation of glucose transmembrane transport Source: UniProtKB
- positive regulation of protein localization to plasma membrane Source: UniProtKB
- positive regulation of protein phosphorylation Source: UniProtKB
- regulation of tumor necrosis factor-mediated signaling pathway Source: Reactome
Enzyme and pathway databases
PathwayCommonsi | Q96DZ5 |
Reactomei | R-HSA-5357905, Regulation of TNFR1 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: CAP-Gly domain-containing linker protein 3Alternative name(s): Cytoplasmic linker protein 170-related 59 kDa protein Short name: CLIP-170-related 59 kDa protein Short name: CLIPR-59 |
Gene namesi | Name:CLIP3 Synonyms:CLIPR59 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24314, CLIP3 |
MIMi | 607382, gene |
neXtProti | NX_Q96DZ5 |
VEuPathDBi | HostDB:ENSG00000105270.14 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Golgi apparatus
- Golgi stack 1 Publication
Other locations
- Cytoplasm 1 Publication
Note: Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.
Cytoskeleton
- microtubule plus-end Source: GO_Central
Cytosol
- cytosol Source: Reactome
Endosome
- early endosome membrane Source: UniProtKB
- recycling endosome membrane Source: UniProtKB
Golgi apparatus
- Golgi stack Source: UniProtKB-SubCell
- trans-Golgi network Source: UniProtKB
- trans-Golgi network membrane Source: UniProtKB
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- membrane raft Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 509 | P → A: Inhibits interaction with ZDHHC13 and ZDHHC17. 1 Publication | 1 | |
Mutagenesisi | 534 – 535 | CC → AA: Strongly reduced plasma membrane association and decrease in the levels of Akt at the plasma membrane. 1 Publication | 2 |
Organism-specific databases
OpenTargetsi | ENSG00000105270 |
PharmGKBi | PA162382439 |
Miscellaneous databases
Pharosi | Q96DZ5, Tbio |
Genetic variation databases
BioMutai | CLIP3 |
DMDMi | 116241302 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076212 | 1 – 547 | CAP-Gly domain-containing linker protein 3Add BLAST | 547 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 374 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 401 | PhosphoserineBy similarity | 1 | |
Lipidationi | 534 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 535 | S-palmitoyl cysteine1 Publication | 1 |
Post-translational modificationi
Palmitoylation by ZDHHC17 regulates association with the plasma membrane.1 Publication
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | Q96DZ5 |
jPOSTi | Q96DZ5 |
MassIVEi | Q96DZ5 |
PaxDbi | Q96DZ5 |
PeptideAtlasi | Q96DZ5 |
PRIDEi | Q96DZ5 |
ProteomicsDBi | 76346 |
PTM databases
iPTMneti | Q96DZ5 |
PhosphoSitePlusi | Q96DZ5 |
SwissPalmi | Q96DZ5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000105270, Expressed in cerebellum and 202 other tissues |
ExpressionAtlasi | Q96DZ5, baseline and differential |
Genevisiblei | Q96DZ5, HS |
Organism-specific databases
HPAi | ENSG00000105270, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ96DZ5
GO - Molecular functioni
- microtubule binding Source: UniProtKB
- microtubule plus-end binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 117475, 13 interactors |
IntActi | Q96DZ5, 10 interactors |
STRINGi | 9606.ENSP00000353732 |
Miscellaneous databases
RNActi | Q96DZ5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96DZ5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96DZ5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 117 – 158 | ANK 1Add BLAST | 42 | |
Repeati | 160 – 191 | ANK 2Add BLAST | 32 | |
Repeati | 197 – 229 | ANK 3Add BLAST | 33 | |
Domaini | 314 – 356 | CAP-Gly 1PROSITE-ProRule annotationAdd BLAST | 43 | |
Domaini | 436 – 478 | CAP-Gly 2PROSITE-ProRule annotationAdd BLAST | 43 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 488 – 547 | GoLDAdd BLAST | 60 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 16 – 25 | Poly-Glu | 10 |
Domaini
Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG4568, Eukaryota |
GeneTreei | ENSGT00940000159557 |
HOGENOMi | CLU_023687_2_1_1 |
InParanoidi | Q96DZ5 |
OMAi | HQVSMSQ |
OrthoDBi | 482616at2759 |
PhylomeDBi | Q96DZ5 |
TreeFami | TF326096 |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit 2.30.30.190, 2 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR030504, CLIP3 |
PANTHERi | PTHR18916:SF77, PTHR18916:SF77, 1 hit |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01302, CAP_GLY, 2 hits |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM01052, CAP_GLY, 2 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF74924, SSF74924, 2 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 1 hit PS00845, CAP_GLY_1, 2 hits PS50245, CAP_GLY_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q96DZ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKTDPAPMA PPPRGEEEEE EEEDEPVPEA PSPTQERRQK PVVHPSAPAP
60 70 80 90 100
LPKDYAFTFF DPNDPACQEI LFDPQTTIPE LFAIVRQWVP QVQHKIDVIG
110 120 130 140 150
NEILRRGCHV NDRDGLTDMT LLHYACKAGA HGVGDPAAAV RLSQQLLALG
160 170 180 190 200
ADVTLRSRWT NMNALHYAAY FDVPDLVRVL LKGARPRVVN STCSDFNHGS
210 220 230 240 250
ALHIAASSLC LGAAKCLLEH GANPALRNRK GQVPAEVVPD PMDMSLDKAE
260 270 280 290 300
AALVAKELRT LLEEAVPLSC ALPKVTLPNY DNVPGNLMLS ALGLRLGDRV
310 320 330 340 350
LLDGQKTGTL RFCGTTEFAS GQWVGVELDE PEGKNDGSVG GVRYFICPPK
360 370 380 390 400
QGLFASVSKI SKAVDAPPSS VTSTPRTPRM DFSRVTGKGR REHKGKKKTP
410 420 430 440 450
SSPSLGSLQQ RDGAKAEVGD QVLVAGQKQG IVRFYGKTDF APGYWYGIEL
460 470 480 490 500
DQPTGKHDGS VFGVRYFTCP PRHGVFAPAS RIQRIGGSTD SPGDSVGAKK
510 520 530 540
VHQVTMTQPK RTFTTVRTPK DIASENSISR LLFCCWFPWM LRAEMQS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketK7ESF3 | K7ESF3_HUMAN | CAP-Gly domain-containing linker pr... | CLIP3 | 133 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027962 | 175 | D → V1 PublicationCorresponds to variant dbSNP:rs17851002Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ427922 mRNA Translation: CAD20873.1 AK094738 mRNA Translation: BAG52921.1 AK289509 mRNA Translation: BAF82198.1 BC013116 mRNA Translation: AAH13116.2 BC014486 mRNA Translation: AAH14486.2 AL117468 mRNA Translation: CAB55943.1 |
CCDSi | CCDS12486.1 |
PIRi | T17253 |
RefSeqi | NP_001186499.1, NM_001199570.1 NP_056341.1, NM_015526.2 |
Genome annotation databases
Ensembli | ENST00000360535; ENSP00000353732; ENSG00000105270 ENST00000593074; ENSP00000466832; ENSG00000105270 |
GeneIDi | 25999 |
KEGGi | hsa:25999 |
UCSCi | uc002ocz.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ427922 mRNA Translation: CAD20873.1 AK094738 mRNA Translation: BAG52921.1 AK289509 mRNA Translation: BAF82198.1 BC013116 mRNA Translation: AAH13116.2 BC014486 mRNA Translation: AAH14486.2 AL117468 mRNA Translation: CAB55943.1 |
CCDSi | CCDS12486.1 |
PIRi | T17253 |
RefSeqi | NP_001186499.1, NM_001199570.1 NP_056341.1, NM_015526.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CP0 | NMR | - | A | 285-366 | [»] | |
SMRi | Q96DZ5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117475, 13 interactors |
IntActi | Q96DZ5, 10 interactors |
STRINGi | 9606.ENSP00000353732 |
PTM databases
iPTMneti | Q96DZ5 |
PhosphoSitePlusi | Q96DZ5 |
SwissPalmi | Q96DZ5 |
Genetic variation databases
BioMutai | CLIP3 |
DMDMi | 116241302 |
Proteomic databases
EPDi | Q96DZ5 |
jPOSTi | Q96DZ5 |
MassIVEi | Q96DZ5 |
PaxDbi | Q96DZ5 |
PeptideAtlasi | Q96DZ5 |
PRIDEi | Q96DZ5 |
ProteomicsDBi | 76346 |
Protocols and materials databases
Antibodypediai | 48030, 129 antibodies |
Genome annotation databases
Ensembli | ENST00000360535; ENSP00000353732; ENSG00000105270 ENST00000593074; ENSP00000466832; ENSG00000105270 |
GeneIDi | 25999 |
KEGGi | hsa:25999 |
UCSCi | uc002ocz.3, human |
Organism-specific databases
CTDi | 25999 |
GeneCardsi | CLIP3 |
HGNCi | HGNC:24314, CLIP3 |
HPAi | ENSG00000105270, Tissue enhanced (brain) |
MIMi | 607382, gene |
neXtProti | NX_Q96DZ5 |
OpenTargetsi | ENSG00000105270 |
PharmGKBi | PA162382439 |
VEuPathDBi | HostDB:ENSG00000105270.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4568, Eukaryota |
GeneTreei | ENSGT00940000159557 |
HOGENOMi | CLU_023687_2_1_1 |
InParanoidi | Q96DZ5 |
OMAi | HQVSMSQ |
OrthoDBi | 482616at2759 |
PhylomeDBi | Q96DZ5 |
TreeFami | TF326096 |
Enzyme and pathway databases
PathwayCommonsi | Q96DZ5 |
Reactomei | R-HSA-5357905, Regulation of TNFR1 signaling |
Miscellaneous databases
BioGRID-ORCSi | 25999, 2 hits in 875 CRISPR screens |
ChiTaRSi | CLIP3, human |
EvolutionaryTracei | Q96DZ5 |
GenomeRNAii | 25999 |
Pharosi | Q96DZ5, Tbio |
PROi | PR:Q96DZ5 |
RNActi | Q96DZ5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000105270, Expressed in cerebellum and 202 other tissues |
ExpressionAtlasi | Q96DZ5, baseline and differential |
Genevisiblei | Q96DZ5, HS |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit 2.30.30.190, 2 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR030504, CLIP3 |
PANTHERi | PTHR18916:SF77, PTHR18916:SF77, 1 hit |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01302, CAP_GLY, 2 hits |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM01052, CAP_GLY, 2 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF74924, SSF74924, 2 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 1 hit PS00845, CAP_GLY_1, 2 hits PS50245, CAP_GLY_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLIP3_HUMAN | |
Accessioni | Q96DZ5Primary (citable) accession number: Q96DZ5 Secondary accession number(s): A8K0E4 Q9UFT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2006 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references