UniProtKB - Q96CM8 (ACSF2_HUMAN)
Medium-chain acyl-CoA ligase ACSF2, mitochondrial
ACSF2
Functioni
Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA (PubMed:17762044).
Has some preference toward medium-chain substrates (PubMed:17762044).
Plays a role in adipocyte differentiation (PubMed:16380219).
2 PublicationsCatalytic activityi
- a medium chain fatty acid + ATP + CoA = a medium-chain fatty acyl-CoA + AMP + diphosphate1 PublicationEC:6.2.1.21 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 493 | ATPBy similarity | 1 | |
Binding sitei | 508 | ATPBy similarity | 1 | |
Binding sitei | 599 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 263 – 271 | ATPBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- butyrate-CoA ligase activity Source: UniProtKB-EC
- long-chain fatty acid-CoA ligase activity Source: Reactome
- medium-chain fatty acid-CoA ligase activity Source: UniProtKB
GO - Biological processi
- acyl-CoA metabolic process Source: Reactome
- fatty acid metabolic process Source: GO_Central
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q96CM8 |
Reactomei | R-HSA-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SignaLinki | Q96CM8 |
Chemistry databases
SwissLipidsi | SLP:000001145 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ACSF2Imported ORF Names:UNQ493/PRO1009 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26101, ACSF2 |
MIMi | 610465, gene |
neXtProti | NX_Q96CM8 |
VEuPathDBi | HostDB:ENSG00000167107 |
Subcellular locationi
Mitochondrion
- Mitochondrion Curated
Mitochondrion
- mitochondrial matrix Source: Reactome
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Organism-specific databases
DisGeNETi | 80221 |
OpenTargetsi | ENSG00000167107 |
PharmGKBi | PA162375338 |
Miscellaneous databases
Pharosi | Q96CM8, Tbio |
Genetic variation databases
BioMutai | ACSF2 |
DMDMi | 166198367 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 41 | MitochondrionSequence analysisAdd BLAST | 41 | |
ChainiPRO_0000315793 | 42 – 615 | Medium-chain acyl-CoA ligase ACSF2, mitochondrialAdd BLAST | 574 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 179 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 182 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 182 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 340 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 398 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 478 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 510 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 544 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 544 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 570 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 570 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 599 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | CPTAC-5 CPTAC-6 |
EPDi | Q96CM8 |
jPOSTi | Q96CM8 |
MassIVEi | Q96CM8 |
MaxQBi | Q96CM8 |
PaxDbi | Q96CM8 |
PeptideAtlasi | Q96CM8 |
PRIDEi | Q96CM8 |
ProteomicsDBi | 4067 4247 5184 76194 [Q96CM8-1] |
TopDownProteomicsi | Q96CM8-1 [Q96CM8-1] |
PTM databases
GlyGeni | Q96CM8, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96CM8 |
PhosphoSitePlusi | Q96CM8 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000167107, Expressed in right adrenal gland and 201 other tissues |
ExpressionAtlasi | Q96CM8, baseline and differential |
Genevisiblei | Q96CM8, HS |
Organism-specific databases
HPAi | ENSG00000167107, Tissue enhanced (kidney) |
Interactioni
Binary interactionsi
Q96CM8
Protein-protein interaction databases
BioGRIDi | 123187, 130 interactors |
IntActi | Q96CM8, 34 interactors |
MINTi | Q96CM8 |
STRINGi | 9606.ENSP00000401831 |
Miscellaneous databases
RNActi | Q96CM8, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1177, Eukaryota |
GeneTreei | ENSGT00940000156830 |
HOGENOMi | CLU_000022_59_7_1 |
InParanoidi | Q96CM8 |
OMAi | KQSDMKA |
OrthoDBi | 386992at2759 |
PhylomeDBi | Q96CM8 |
TreeFami | TF313466 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVYVGMLRL GRLCAGSSGV LGARAALSRS WQEARLQGVR FLSSREVDRM
60 70 80 90 100
VSTPIGGLSY VQGCTKKHLN SKTVGQCLET TAQRVPEREA LVVLHEDVRL
110 120 130 140 150
TFAQLKEEVD KAASGLLSIG LCKGDRLGMW GPNSYAWVLM QLATAQAGII
160 170 180 190 200
LVSVNPAYQA MELEYVLKKV GCKALVFPKQ FKTQQYYNVL KQICPEVENA
210 220 230 240 250
QPGALKSQRL PDLTTVISVD APLPGTLLLD EVVAAGSTRQ HLDQLQYNQQ
260 270 280 290 300
FLSCHDPINI QFTSGTTGSP KGATLSHYNI VNNSNILGER LKLHEKTPEQ
310 320 330 340 350
LRMILPNPLY HCLGSVAGTM MCLMYGATLI LASPIFNGKK ALEAISRERG
360 370 380 390 400
TFLYGTPTMF VDILNQPDFS SYDISTMCGG VIAGSPAPPE LIRAIINKIN
410 420 430 440 450
MKDLVVAYGT TENSPVTFAH FPEDTVEQKA ESVGRIMPHT EARIMNMEAG
460 470 480 490 500
TLAKLNTPGE LCIRGYCVML GYWGEPQKTE EAVDQDKWYW TGDVATMNEQ
510 520 530 540 550
GFCKIVGRSK DMIIRGGENI YPAELEDFFH THPKVQEVQV VGVKDDRMGE
560 570 580 590 600
EICACIRLKD GEETTVEEIK AFCKGKISHF KIPKYIVFVT NYPLTISGKI
610
QKFKLREQME RHLNL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PF16 | E9PF16_HUMAN | Medium-chain acyl-CoA ligase ACSF2,... | ACSF2 | 572 | Annotation score: | ||
D6RF87 | D6RF87_HUMAN | Medium-chain acyl-CoA ligase ACSF2,... | ACSF2 | 183 | Annotation score: | ||
H0Y8N1 | H0Y8N1_HUMAN | Medium-chain acyl-CoA ligase ACSF2,... | ACSF2 | 104 | Annotation score: | ||
H0Y8Y5 | H0Y8Y5_HUMAN | Medium-chain acyl-CoA ligase ACSF2,... | ACSF2 | 85 | Annotation score: | ||
D6RF15 | D6RF15_HUMAN | Medium-chain acyl-CoA ligase ACSF2,... | ACSF2 | 54 | Annotation score: | ||
E7EN53 | E7EN53_HUMAN | Medium-chain acyl-CoA ligase ACSF2,... | ACSF2 | 45 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_038304 | 75 | G → V1 PublicationCorresponds to variant dbSNP:rs17856448Ensembl. | 1 | |
Natural variantiVAR_038305 | 316 | V → M. Corresponds to variant dbSNP:rs3744523Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055803 | 1 – 160 | Missing in isoform 4. 1 PublicationAdd BLAST | 160 | |
Alternative sequenceiVSP_055804 | 43 | S → SARGGMEAGRQRISVPSSFT ASAAAH in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_055805 | 146 – 158 | Missing in isoform 3. 1 PublicationAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY358660 mRNA Translation: AAQ89023.1 AK024573 mRNA Translation: BAB14930.1 AK294205 mRNA Translation: BAG57517.1 AK295258 mRNA Translation: BAG58247.1 AK300625 mRNA Translation: BAG62317.1 AC004707 Genomic DNA No translation available. AC021491 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94612.1 BC012053 mRNA Translation: AAH12053.1 BC014123 mRNA Translation: AAH14123.1 |
CCDSi | CCDS11567.1 [Q96CM8-1] CCDS74103.1 [Q96CM8-2] CCDS74104.1 [Q96CM8-3] |
RefSeqi | NP_001275897.1, NM_001288968.1 [Q96CM8-2] NP_001275898.1, NM_001288969.1 [Q96CM8-3] NP_001275899.1, NM_001288970.1 NP_001275900.1, NM_001288971.1 [Q96CM8-4] NP_001275901.1, NM_001288972.1 [Q96CM8-4] NP_079425.3, NM_025149.5 [Q96CM8-1] |
Genome annotation databases
Ensembli | ENST00000300441.9; ENSP00000300441.4; ENSG00000167107.13 ENST00000427954.6; ENSP00000401831.2; ENSG00000167107.13 [Q96CM8-2] ENST00000502667.5; ENSP00000421884.1; ENSG00000167107.13 [Q96CM8-3] |
GeneIDi | 80221 |
KEGGi | hsa:80221 |
MANE-Selecti | ENST00000300441.9; ENSP00000300441.4; NM_025149.6; NP_079425.3 |
UCSCi | uc002iqu.4, human [Q96CM8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY358660 mRNA Translation: AAQ89023.1 AK024573 mRNA Translation: BAB14930.1 AK294205 mRNA Translation: BAG57517.1 AK295258 mRNA Translation: BAG58247.1 AK300625 mRNA Translation: BAG62317.1 AC004707 Genomic DNA No translation available. AC021491 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94612.1 BC012053 mRNA Translation: AAH12053.1 BC014123 mRNA Translation: AAH14123.1 |
CCDSi | CCDS11567.1 [Q96CM8-1] CCDS74103.1 [Q96CM8-2] CCDS74104.1 [Q96CM8-3] |
RefSeqi | NP_001275897.1, NM_001288968.1 [Q96CM8-2] NP_001275898.1, NM_001288969.1 [Q96CM8-3] NP_001275899.1, NM_001288970.1 NP_001275900.1, NM_001288971.1 [Q96CM8-4] NP_001275901.1, NM_001288972.1 [Q96CM8-4] NP_079425.3, NM_025149.5 [Q96CM8-1] |
3D structure databases
AlphaFoldDBi | Q96CM8 |
SMRi | Q96CM8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123187, 130 interactors |
IntActi | Q96CM8, 34 interactors |
MINTi | Q96CM8 |
STRINGi | 9606.ENSP00000401831 |
Chemistry databases
SwissLipidsi | SLP:000001145 |
PTM databases
GlyGeni | Q96CM8, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q96CM8 |
PhosphoSitePlusi | Q96CM8 |
Genetic variation databases
BioMutai | ACSF2 |
DMDMi | 166198367 |
Proteomic databases
CPTACi | CPTAC-5 CPTAC-6 |
EPDi | Q96CM8 |
jPOSTi | Q96CM8 |
MassIVEi | Q96CM8 |
MaxQBi | Q96CM8 |
PaxDbi | Q96CM8 |
PeptideAtlasi | Q96CM8 |
PRIDEi | Q96CM8 |
ProteomicsDBi | 4067 4247 5184 76194 [Q96CM8-1] |
TopDownProteomicsi | Q96CM8-1 [Q96CM8-1] |
Protocols and materials databases
Antibodypediai | 30532, 137 antibodies from 24 providers |
DNASUi | 80221 |
Genome annotation databases
Ensembli | ENST00000300441.9; ENSP00000300441.4; ENSG00000167107.13 ENST00000427954.6; ENSP00000401831.2; ENSG00000167107.13 [Q96CM8-2] ENST00000502667.5; ENSP00000421884.1; ENSG00000167107.13 [Q96CM8-3] |
GeneIDi | 80221 |
KEGGi | hsa:80221 |
MANE-Selecti | ENST00000300441.9; ENSP00000300441.4; NM_025149.6; NP_079425.3 |
UCSCi | uc002iqu.4, human [Q96CM8-1] |
Organism-specific databases
CTDi | 80221 |
DisGeNETi | 80221 |
GeneCardsi | ACSF2 |
HGNCi | HGNC:26101, ACSF2 |
HPAi | ENSG00000167107, Tissue enhanced (kidney) |
MIMi | 610465, gene |
neXtProti | NX_Q96CM8 |
OpenTargetsi | ENSG00000167107 |
PharmGKBi | PA162375338 |
VEuPathDBi | HostDB:ENSG00000167107 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1177, Eukaryota |
GeneTreei | ENSGT00940000156830 |
HOGENOMi | CLU_000022_59_7_1 |
InParanoidi | Q96CM8 |
OMAi | KQSDMKA |
OrthoDBi | 386992at2759 |
PhylomeDBi | Q96CM8 |
TreeFami | TF313466 |
Enzyme and pathway databases
PathwayCommonsi | Q96CM8 |
Reactomei | R-HSA-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SignaLinki | Q96CM8 |
Miscellaneous databases
BioGRID-ORCSi | 80221, 18 hits in 1079 CRISPR screens |
ChiTaRSi | ACSF2, human |
GenomeRNAii | 80221 |
Pharosi | Q96CM8, Tbio |
PROi | PR:Q96CM8 |
RNActi | Q96CM8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167107, Expressed in right adrenal gland and 201 other tissues |
ExpressionAtlasi | Q96CM8, baseline and differential |
Genevisiblei | Q96CM8, HS |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACSF2_HUMAN | |
Accessioni | Q96CM8Primary (citable) accession number: Q96CM8 Secondary accession number(s): B4DFQ6 Q9H7G2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families