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Protein

ELL-associated factor 2

Gene

EAF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processApoptosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation
SIGNORiQ96CJ1

Names & Taxonomyi

Protein namesi
Recommended name:
ELL-associated factor 2
Alternative name(s):
Testosterone-regulated apoptosis inducer and tumor suppressor protein
Gene namesi
Name:EAF2
Synonyms:TRAITS
ORF Names:BM-040
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000145088.8
HGNCiHGNC:23115 EAF2
MIMi607659 gene
neXtProtiNX_Q96CJ1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55840
OpenTargetsiENSG00000145088
PharmGKBiPA128394690

Polymorphism and mutation databases

BioMutaiEAF2
DMDMi73919269

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001303371 – 260ELL-associated factor 2Add BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei146PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1
Modified residuei154PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96CJ1
MaxQBiQ96CJ1
PaxDbiQ96CJ1
PeptideAtlasiQ96CJ1
PRIDEiQ96CJ1
ProteomicsDBi76189
76190 [Q96CJ1-2]

PTM databases

iPTMnetiQ96CJ1
PhosphoSitePlusiQ96CJ1

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.2 Publications

Inductioni

By androgen.1 Publication

Gene expression databases

BgeeiENSG00000145088 Expressed in 184 organ(s), highest expression level in bone marrow
CleanExiHS_EAF2
ExpressionAtlasiQ96CJ1 baseline and differential
GenevisibleiQ96CJ1 HS

Organism-specific databases

HPAiHPA008411

Interactioni

Subunit structurei

Isoform 1 and isoform 2 interact with TCEA1 (By similarity). Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL and ELL2.By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi120943, 17 interactors
CORUMiQ96CJ1
IntActiQ96CJ1, 22 interactors
MINTiQ96CJ1
STRINGi9606.ENSP00000273668

Structurei

3D structure databases

ProteinModelPortaliQ96CJ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 104Necessary for interaction with ELL1 PublicationAdd BLAST88
Regioni177 – 260Necessary for transactivation activityAdd BLAST84
Regioni246 – 260Necessary for interaction with TCEA1 and transactivation activityBy similarityAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi126 – 130Poly-Gln5
Compositional biasi174 – 207Ser-richAdd BLAST34

Sequence similaritiesi

Belongs to the EAF family.Curated

Phylogenomic databases

eggNOGiKOG4795 Eukaryota
ENOG4111KQJ LUCA
GeneTreeiENSGT00390000017724
HOGENOMiHOG000070137
HOVERGENiHBG054898
InParanoidiQ96CJ1
KOiK15186
PhylomeDBiQ96CJ1
TreeFamiTF320864

Family and domain databases

InterProiView protein in InterPro
IPR027093 EAF_fam
IPR019194 Tscrpt_elong_fac_Eaf_N
PANTHERiPTHR15970 PTHR15970, 1 hit
PfamiView protein in Pfam
PF09816 EAF, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96CJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY
60 70 80 90 100
LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL
110 120 130 140 150
EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM
160 170 180 190 200
SPASPIDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS EDSSSDSEDE
210 220 230 240 250
DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL LMNTLRNDLQ
260
LSESGSDSDD
Length:260
Mass (Da):28,792
Last modified:December 1, 2001 - v1
Checksum:i2CDCE96FA387C799
GO
Isoform 2 (identifier: Q96CJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: Missing.

Show »
Length:130
Mass (Da):14,091
Checksum:iAEDE38BF25A5014B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4DWJ3B4DWJ3_HUMAN
cDNA FLJ60185, highly similar to EL...
EAF2
263Annotation score:
F8WCI9F8WCI9_HUMAN
ELL-associated factor 2
EAF2
68Annotation score:
F8WF04F8WF04_HUMAN
ELL-associated factor 2
EAF2
35Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0153101 – 130Missing in isoform 2. 1 PublicationAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF517829 mRNA Translation: AAO63811.1
AY049020 mRNA Translation: AAL12223.1
AF217516 mRNA Translation: AAF67627.1
BC014209 mRNA Translation: AAH14209.1
CCDSiCCDS3006.1 [Q96CJ1-1]
RefSeqiNP_001306970.1, NM_001320041.1 [Q96CJ1-2]
NP_060926.2, NM_018456.5 [Q96CJ1-1]
XP_016862350.1, XM_017006861.1 [Q96CJ1-2]
XP_016862351.1, XM_017006862.1 [Q96CJ1-2]
UniGeneiHs.477325

Genome annotation databases

EnsembliENST00000273668; ENSP00000273668; ENSG00000145088 [Q96CJ1-1]
GeneIDi55840
KEGGihsa:55840
UCSCiuc003een.4 human [Q96CJ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF517829 mRNA Translation: AAO63811.1
AY049020 mRNA Translation: AAL12223.1
AF217516 mRNA Translation: AAF67627.1
BC014209 mRNA Translation: AAH14209.1
CCDSiCCDS3006.1 [Q96CJ1-1]
RefSeqiNP_001306970.1, NM_001320041.1 [Q96CJ1-2]
NP_060926.2, NM_018456.5 [Q96CJ1-1]
XP_016862350.1, XM_017006861.1 [Q96CJ1-2]
XP_016862351.1, XM_017006862.1 [Q96CJ1-2]
UniGeneiHs.477325

3D structure databases

ProteinModelPortaliQ96CJ1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120943, 17 interactors
CORUMiQ96CJ1
IntActiQ96CJ1, 22 interactors
MINTiQ96CJ1
STRINGi9606.ENSP00000273668

PTM databases

iPTMnetiQ96CJ1
PhosphoSitePlusiQ96CJ1

Polymorphism and mutation databases

BioMutaiEAF2
DMDMi73919269

Proteomic databases

EPDiQ96CJ1
MaxQBiQ96CJ1
PaxDbiQ96CJ1
PeptideAtlasiQ96CJ1
PRIDEiQ96CJ1
ProteomicsDBi76189
76190 [Q96CJ1-2]

Protocols and materials databases

DNASUi55840
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273668; ENSP00000273668; ENSG00000145088 [Q96CJ1-1]
GeneIDi55840
KEGGihsa:55840
UCSCiuc003een.4 human [Q96CJ1-1]

Organism-specific databases

CTDi55840
DisGeNETi55840
EuPathDBiHostDB:ENSG00000145088.8
GeneCardsiEAF2
HGNCiHGNC:23115 EAF2
HPAiHPA008411
MIMi607659 gene
neXtProtiNX_Q96CJ1
OpenTargetsiENSG00000145088
PharmGKBiPA128394690
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4795 Eukaryota
ENOG4111KQJ LUCA
GeneTreeiENSGT00390000017724
HOGENOMiHOG000070137
HOVERGENiHBG054898
InParanoidiQ96CJ1
KOiK15186
PhylomeDBiQ96CJ1
TreeFamiTF320864

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation
SIGNORiQ96CJ1

Miscellaneous databases

ChiTaRSiEAF2 human
GeneWikiiEAF2
GenomeRNAii55840
PROiPR:Q96CJ1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000145088 Expressed in 184 organ(s), highest expression level in bone marrow
CleanExiHS_EAF2
ExpressionAtlasiQ96CJ1 baseline and differential
GenevisibleiQ96CJ1 HS

Family and domain databases

InterProiView protein in InterPro
IPR027093 EAF_fam
IPR019194 Tscrpt_elong_fac_Eaf_N
PANTHERiPTHR15970 PTHR15970, 1 hit
PfamiView protein in Pfam
PF09816 EAF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEAF2_HUMAN
AccessioniPrimary (citable) accession number: Q96CJ1
Secondary accession number(s): Q9NZ82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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