Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable ATP-dependent RNA helicase DHX58

Gene

DHX58

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.10 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi556ZincPROSITE-ProRule annotation1
Metal bindingi559ZincPROSITE-ProRule annotation1
Metal bindingi612ZincPROSITE-ProRule annotation1
Metal bindingi615ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processAntiviral defense, Host-virus interaction, Immunity, Innate immunity
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHX58 (EC:3.6.4.13)
Alternative name(s):
Probable ATP-dependent helicase LGP2
Protein D11Lgp2 homolog
RIG-I-like receptor 3
Short name:
RLR-3
RIG-I-like receptor LGP2
Short name:
RLR
Gene namesi
Name:DHX58
Synonyms:D11LGP2E, LGP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108771.12
HGNCiHGNC:29517 DHX58
MIMi608588 gene
neXtProtiNX_Q96C10

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi634K → E: Abolishes RNA binding. 2 Publications1
Mutagenesisi651K → E: Abolishes RNA binding. 1 Publication1

Organism-specific databases

DisGeNETi79132
OpenTargetsiENSG00000108771
PharmGKBiPA162383566

Polymorphism and mutation databases

BioMutaiDHX58
DMDMi50401123

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020101 – 678Probable ATP-dependent RNA helicase DHX58Add BLAST678

Proteomic databases

MaxQBiQ96C10
PaxDbiQ96C10
PeptideAtlasiQ96C10
PRIDEiQ96C10
ProteomicsDBi76141
TopDownProteomicsiQ96C10

PTM databases

iPTMnetiQ96C10
PhosphoSitePlusiQ96C10

Expressioni

Inductioni

By interferon (IFN), virus infection, or intracellular dsRNA.

Gene expression databases

BgeeiENSG00000108771 Expressed in 105 organ(s), highest expression level in left adrenal gland
CleanExiHS_DHX58
ExpressionAtlasiQ96C10 baseline and differential
GenevisibleiQ96C10 HS

Organism-specific databases

HPAiHPA018670
HPA019570

Interactioni

Subunit structurei

Monomer in the absence of dsRNA. Homodimer in the presence of dsRNA. Interacts with DDX58/RIG-I (via CARD domain), MAVS/IPS1 and DDX60. Found in a complex with DDX58/RIG-I and IFIH1/MDA5. Interacts with ANKRD17. Directly interacts with ATG5 and ATG12, either as ATG5 and ATG12 monomers or as ATG12-ATG5 conjugates (PubMed:17709747).8 Publications
(Microbial infection) Interacts (via helicase C-terminal domain) with non-structural protein V of paramyxoviruses including human parainfluenza 2 virus, human parainfluenza 5 virus, measles virus, mumps virus, hendra virus and nipah virus.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi122553, 13 interactors
DIPiDIP-60792N
IntActiQ96C10, 20 interactors
STRINGi9606.ENSP00000251642

Structurei

Secondary structure

1678
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96C10
SMRiQ96C10
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96C10

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 188Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST178
Domaini350 – 514Helicase C-terminalPROSITE-ProRule annotationAdd BLAST165
Domaini539 – 669RLR CTRPROSITE-ProRule annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni572 – 655RNA-bindingAdd BLAST84

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili489 – 546Sequence analysisAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi131 – 134DECH box4

Domaini

The RLR CTR domain is capable of inhibiting dimerization and signaling of DDX58/RIG-I and also facilitates binding of dsRNA.

Sequence similaritiesi

Belongs to the helicase family. RLR subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0354 Eukaryota
COG1111 LUCA
GeneTreeiENSGT00510000046789
HOGENOMiHOG000230992
HOVERGENiHBG106019
InParanoidiQ96C10
KOiK12649
OMAiFYEPVPS
OrthoDBiEOG091G01PQ
PhylomeDBiQ96C10
TreeFamiTF330258

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di2.170.150.30, 1 hit
InterProiView protein in InterPro
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038557 RLR_C_sf
IPR021673 RLR_CTR
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
PF11648 RIG-I_C-RD, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51789 RLR_CTR, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.iShow all

Q96C10-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELRSYQWEV IMPALEGKNI IIWLPTGAGK TRAAAYVAKR HLETVDGAKV
60 70 80 90 100
VVLVNRVHLV TQHGEEFRRM LDGRWTVTTL SGDMGPRAGF GHLARCHDLL
110 120 130 140 150
ICTAELLQMA LTSPEEEEHV ELTVFSLIVV DECHHTHKDT VYNVIMSQYL
160 170 180 190 200
ELKLQRAQPL PQVLGLTASP GTGGASKLDG AINHVLQLCA NLDTWCIMSP
210 220 230 240 250
QNCCPQLQEH SQQPCKQYNL CHRRSQDPFG DLLKKLMDQI HDHLEMPELS
260 270 280 290 300
RKFGTQMYEQ QVVKLSEAAA LAGLQEQRVY ALHLRRYNDA LLIHDTVRAV
310 320 330 340 350
DALAALQDFY HREHVTKTQI LCAERRLLAL FDDRKNELAH LATHGPENPK
360 370 380 390 400
LEMLEKILQR QFSSSNSPRG IIFTRTRQSA HSLLLWLQQQ QGLQTVDIRA
410 420 430 440 450
QLLIGAGNSS QSTHMTQRDQ QEVIQKFQDG TLNLLVATSV AEEGLDIPHC
460 470 480 490 500
NVVVRYGLLT NEISMVQARG RARADQSVYA FVATEGSREL KRELINEALE
510 520 530 540 550
TLMEQAVAAV QKMDQAEYQA KIRDLQQAAL TKRAAQAAQR ENQRQQFPVE
560 570 580 590 600
HVQLLCINCM VAVGHGSDLR KVEGTHHVNV NPNFSNYYNV SRDPVVINKV
610 620 630 640 650
FKDWKPGGVI SCRNCGEVWG LQMIYKSVKL PVLKVRSMLL ETPQGRIQAK
660 670
KWSRVPFSVP DFDFLQHCAE NLSDLSLD
Length:678
Mass (Da):76,613
Last modified:December 1, 2001 - v1
Checksum:i859E1749C7313D06
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JG98C9JG98_HUMAN
Probable ATP-dependent RNA helicase...
DHX58
302Annotation score:
C9JE76C9JE76_HUMAN
Probable ATP-dependent RNA helicase...
DHX58
156Annotation score:
K7EPP0K7EPP0_HUMAN
Probable ATP-dependent RNA helicase...
DHX58
51Annotation score:
K7EMZ5K7EMZ5_HUMAN
Probable ATP-dependent RNA helicase...
DHX58
99Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti473R → W in BAB13818 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04933676T → A. Corresponds to variant dbSNP:rs34891485Ensembl.1
Natural variantiVAR_04933795R → Q. Corresponds to variant dbSNP:rs35118457Ensembl.1
Natural variantiVAR_019394425Q → R. Corresponds to variant dbSNP:rs2074158Ensembl.1
Natural variantiVAR_019395523R → Q. Corresponds to variant dbSNP:rs2074160Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK021416 mRNA Translation: BAB13818.1
BC014949 mRNA Translation: AAH14949.1
CCDSiCCDS11416.1
RefSeqiNP_077024.2, NM_024119.2
XP_016880548.1, XM_017025059.1
XP_016880549.1, XM_017025060.1
UniGeneiHs.55918

Genome annotation databases

EnsembliENST00000251642; ENSP00000251642; ENSG00000108771
GeneIDi79132
KEGGihsa:79132
UCSCiuc002hyw.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDHX58_HUMAN
AccessioniPrimary (citable) accession number: Q96C10
Secondary accession number(s): Q9HAM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again