UniProtKB - Q96BY2 (MOAP1_HUMAN)
Modulator of apoptosis 1
MOAP1
Functioni
Retrotransposon-derived protein that forms virion-like capsids (By similarity).
Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525).
Required for death receptor-dependent apoptosis (PubMed:11060313).
When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439).
Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314).
Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215).
By similarity5 PublicationsGO - Molecular functioni
- ubiquitin protein ligase binding Source: BHF-UCL
GO - Biological processi
- apoptotic signaling pathway Source: BHF-UCL
- extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
- extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
- intrinsic apoptotic signaling pathway in response to DNA damage Source: BHF-UCL
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of autophagosome assembly Source: UniProtKB
- positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
- protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: BHF-UCL
- regulation of apoptotic process Source: GO_Central
Keywordsi
Biological process | Apoptosis, Autophagy |
Enzyme and pathway databases
PathwayCommonsi | Q96BY2 |
SignaLinki | Q96BY2 |
Names & Taxonomyi
Protein namesi | Recommended name: Modulator of apoptosis 11 PublicationShort name: MAP-11 Publication Short name: MAP11 Publication Alternative name(s): Paraneoplastic antigen Ma41 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16658, MOAP1 |
MIMi | 609485, gene |
neXtProti | NX_Q96BY2 |
VEuPathDBi | HostDB:ENSG00000165943 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 1 Publication
Cytoplasm and Cytosol
- cytosol 1 Publication
Other locations
- Extracellular vesicle membrane By similarity
Note: Forms virion-like extracellular vesicles that are released from cells.By similarity
Cytosol
- cytosol Source: BHF-UCL
Mitochondrion
- mitochondrial outer membrane Source: BHF-UCL
- mitochondrion Source: BHF-UCL
Other locations
- cytoplasm Source: LIFEdb
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 49 – 52 | YRLL → ARLA: Abolished interaction with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C. 1 Publication | 4 | |
Mutagenesisi | 89 – 92 | WRVI → ARVA: Does not affect interaction with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C. 1 Publication | 4 | |
Mutagenesisi | 120 – 127 | Missing : Abrogates interaction with BAX, resulting in a nonapoptotic protein. 1 Publication | 8 | |
Mutagenesisi | 120 | L → E: Weakened interaction with BAX, resulting in a nonapoptotic protein. 1 Publication | 1 | |
Mutagenesisi | 125 – 127 | GHE → VLA: Abrogates interaction with BAX, resulting in a nonapoptotic protein. 1 Publication | 3 | |
Mutagenesisi | 161 – 166 | KYKKLR → AYAALA: No effect on RASSF1-binding. 1 Publication | 6 | |
Mutagenesisi | 162 – 165 | YKKL → AKKA: Does not affect interaction with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C. 1 Publication | 4 | |
Mutagenesisi | 178 – 180 | EEE → AAA: No effect on RASSF1-binding; interacts with BAX in the absence of RASSF1. | 3 | |
Mutagenesisi | 202 – 205 | KRRR → AAAA: Loss of RASSF1-binding; interacts with BAX in the absence of RASSF1. 1 Publication | 4 |
Organism-specific databases
DisGeNETi | 64112 |
OpenTargetsi | ENSG00000165943 |
PharmGKBi | PA134908381 |
Miscellaneous databases
Pharosi | Q96BY2, Tbio |
Genetic variation databases
BioMutai | MOAP1 |
DMDMi | 37999755 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000155205 | 1 – 351 | Modulator of apoptosis 1Add BLAST | 351 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
MassIVEi | Q96BY2 |
MaxQBi | Q96BY2 |
PaxDbi | Q96BY2 |
PeptideAtlasi | Q96BY2 |
PRIDEi | Q96BY2 |
ProteomicsDBi | 76126 |
PTM databases
iPTMneti | Q96BY2 |
PhosphoSitePlusi | Q96BY2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000165943, Expressed in parietal lobe and 247 other tissues |
Genevisiblei | Q96BY2, HS |
Organism-specific databases
HPAi | ENSG00000165943, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (PubMed:15949439). Under normal circumstances, held in an inactive conformation by an intramolecular interaction (PubMed:15949439).
Interacts with BAX (PubMed:11060313, PubMed:16199525). Binding to RASSF1 isoform A (RASSF1A) relieves this inhibitory interaction and allows further binding to BAX (PubMed:15949439). Binds also to BCL2 and BCLX (PubMed:11060313). Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization (PubMed:15949439).
Interacts with TRIM39 (PubMed:19100260).
Interacts with RASSF6 (By similarity).
Interacts with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C (PubMed:33783314). Does not interact with ATG8 proteins GABARAPL1, GABARAPL2 and GABARAP (PubMed:33783314).
Interacts with SQSTM1; promoting dissociation of SQSTM1 inclusion bodies that sequester KEAP1 (PubMed:33393215).
By similarity6 PublicationsBinary interactionsi
Q96BY2
GO - Molecular functioni
- ubiquitin protein ligase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 122069, 37 interactors |
DIPi | DIP-49959N |
IntActi | Q96BY2, 31 interactors |
MINTi | Q96BY2 |
STRINGi | 9606.ENSP00000451594 |
Miscellaneous databases
RNActi | Q96BY2, protein |
Structurei
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 120 – 127 | BH3-like1 Publication | 8 | |
Regioni | 202 – 205 | RASSF1-binding1 Publication | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 49 – 52 | LIR1 Publication | 4 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502SAVD, Eukaryota |
GeneTreei | ENSGT01030000234522 |
HOGENOMi | CLU_014694_0_0_1 |
InParanoidi | Q96BY2 |
OMAi | GMIPEIR |
OrthoDBi | 820525at2759 |
PhylomeDBi | Q96BY2 |
TreeFami | TF335054 |
Family and domain databases
InterProi | View protein in InterPro IPR026523, PNMA |
PANTHERi | PTHR23095, PTHR23095, 1 hit |
Pfami | View protein in Pfam PF14893, PNMA, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTLRLLEDWC RGMDMNPRKA LLIAGISQSC SVAEIEEALQ AGLAPLGEYR
60 70 80 90 100
LLGRMFRRDE NRKVALVGLT AETSHALVPK EIPGKGGIWR VIFKPPDPDN
110 120 130 140 150
TFLSRLNEFL AGEGMTVGEL SRALGHENGS LDPEQGMIPE MWAPMLAQAL
160 170 180 190 200
EALQPALQCL KYKKLRVFSG RESPEPGEEE FGRWMFHTTQ MIKAWQVPDV
210 220 230 240 250
EKRRRLLESL RGPALDVIRV LKINNPLITV DECLQALEEV FGVTDNPREL
260 270 280 290 300
QVKYLTTYQK DEEKLSAYVL RLEPLLQKLV QRGAIERDAV NQARLDQVIA
310 320 330 340 350
GAVHKTIRRE LNLPEDGPAP GFLQLLVLIK DYEAAEEEEA LLQAILEGNF
T
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 244 | T → A in BAB14788 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 258 | Y → H in BAB14788 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 259 | Q → H in AAG31786 (PubMed:11060313).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF305550 mRNA Translation: AAG31786.1 AK024029 mRNA Translation: BAB14788.1 Sequence problems. AK315522 mRNA Translation: BAG37903.1 CH471061 Genomic DNA Translation: EAW81516.1 BC015044 mRNA Translation: AAH15044.1 |
CCDSi | CCDS9908.1 |
RefSeqi | NP_071434.2, NM_022151.4 |
Genome annotation databases
Ensembli | ENST00000298894; ENSP00000298894; ENSG00000165943 ENST00000556883; ENSP00000451594; ENSG00000165943 ENST00000616515; ENSP00000482284; ENSG00000278268 ENST00000627896; ENSP00000486260; ENSG00000278268 |
GeneIDi | 64112 |
KEGGi | hsa:64112 |
MANE-Selecti | ENST00000298894.5; ENSP00000298894.4; NM_022151.5; NP_071434.2 |
UCSCi | uc001ybj.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF305550 mRNA Translation: AAG31786.1 AK024029 mRNA Translation: BAB14788.1 Sequence problems. AK315522 mRNA Translation: BAG37903.1 CH471061 Genomic DNA Translation: EAW81516.1 BC015044 mRNA Translation: AAH15044.1 |
CCDSi | CCDS9908.1 |
RefSeqi | NP_071434.2, NM_022151.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7LGC | X-ray | 1.85 | A/B | 242-351 | [»] | |
ModBasei | Search... | |||||
SWISS-MODEL-Workspacei | Submit a new modelling project... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122069, 37 interactors |
DIPi | DIP-49959N |
IntActi | Q96BY2, 31 interactors |
MINTi | Q96BY2 |
STRINGi | 9606.ENSP00000451594 |
PTM databases
iPTMneti | Q96BY2 |
PhosphoSitePlusi | Q96BY2 |
Genetic variation databases
BioMutai | MOAP1 |
DMDMi | 37999755 |
Proteomic databases
MassIVEi | Q96BY2 |
MaxQBi | Q96BY2 |
PaxDbi | Q96BY2 |
PeptideAtlasi | Q96BY2 |
PRIDEi | Q96BY2 |
ProteomicsDBi | 76126 |
Protocols and materials databases
Antibodypediai | 22, 318 antibodies from 30 providers |
DNASUi | 64112 |
Genome annotation databases
Ensembli | ENST00000298894; ENSP00000298894; ENSG00000165943 ENST00000556883; ENSP00000451594; ENSG00000165943 ENST00000616515; ENSP00000482284; ENSG00000278268 ENST00000627896; ENSP00000486260; ENSG00000278268 |
GeneIDi | 64112 |
KEGGi | hsa:64112 |
MANE-Selecti | ENST00000298894.5; ENSP00000298894.4; NM_022151.5; NP_071434.2 |
UCSCi | uc001ybj.4, human |
Organism-specific databases
CTDi | 64112 |
DisGeNETi | 64112 |
GeneCardsi | MOAP1 |
HGNCi | HGNC:16658, MOAP1 |
HPAi | ENSG00000165943, Low tissue specificity |
MIMi | 609485, gene |
neXtProti | NX_Q96BY2 |
OpenTargetsi | ENSG00000165943 |
PharmGKBi | PA134908381 |
VEuPathDBi | HostDB:ENSG00000165943 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SAVD, Eukaryota |
GeneTreei | ENSGT01030000234522 |
HOGENOMi | CLU_014694_0_0_1 |
InParanoidi | Q96BY2 |
OMAi | GMIPEIR |
OrthoDBi | 820525at2759 |
PhylomeDBi | Q96BY2 |
TreeFami | TF335054 |
Enzyme and pathway databases
PathwayCommonsi | Q96BY2 |
SignaLinki | Q96BY2 |
Miscellaneous databases
BioGRID-ORCSi | 64112, 10 hits in 1038 CRISPR screens |
ChiTaRSi | MOAP1, human |
GeneWikii | MOAP1 |
GenomeRNAii | 64112 |
Pharosi | Q96BY2, Tbio |
PROi | PR:Q96BY2 |
RNActi | Q96BY2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000165943, Expressed in parietal lobe and 247 other tissues |
Genevisiblei | Q96BY2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026523, PNMA |
PANTHERi | PTHR23095, PTHR23095, 1 hit |
Pfami | View protein in Pfam PF14893, PNMA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MOAP1_HUMAN | |
Accessioni | Q96BY2Primary (citable) accession number: Q96BY2 Secondary accession number(s): B2RDF6, Q9H833, Q9HAS1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families