Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 160 (26 Feb 2020)
Sequence version 2 (20 May 2008)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

Alkylated DNA repair protein alkB homolog 8

Gene

ALKBH8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323, PubMed:31079898). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).5 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Fe2+1 Publication1 PublicationNote: Binds 1 Fe2+ ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi238Iron; catalyticPROSITE-ProRule annotationCurated1
Metal bindingi240Iron; catalyticPROSITE-ProRule annotationCurated1
Metal bindingi242Zinc; via pros nitrogen1 Publication1
Metal bindingi292Iron; catalyticPROSITE-ProRule annotationCurated1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei328Alpha-ketoglutarate1 Publication1
Binding sitei334Alpha-ketoglutarate1 Publication1
Metal bindingi341Zinc1 Publication1
Metal bindingi343Zinc1 Publication1
Metal bindingi349Zinc1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Oxidoreductase, RNA-binding, Transferase
LigandIron, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:ENSG00000137760-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.1.1.229 2681

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-6782315 tRNA modification in the nucleus and cytosol

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alkylated DNA repair protein alkB homolog 8 (EC:1.14.11.-)
Alternative name(s):
Probable alpha-ketoglutarate-dependent dioxygenase ABH8
S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 (EC:2.1.1.2293 Publications)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ALKBH8
Synonyms:ABH8
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11

Organism-specific databases

Human Gene Nomenclature Database

More...
HGNCi
HGNC:25189 ALKBH8

Online Mendelian Inheritance in Man (OMIM)

More...
MIMi
613306 gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q96BT7

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Intellectual developmental disorder, autosomal recessive 71 (MRT71)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Intellectual deficiency is the only primary symptom of non-syndromic mental retardation, while syndromic mental retardation presents with associated physical, neurological and/or psychiatric manifestations. MRT71 features include impaired intellectual development, global developmental delay, mildly delayed walking, poor language, seizures in the first years of life, and behavioral abnormalities.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_083119554 – 664Missing in MRT71; missing tRNA wobble uridine modification. 1 PublicationAdd BLAST111

Keywords - Diseasei

Disease mutation, Mental retardation

Organism-specific databases

DisGeNET

More...
DisGeNETi
91801

MalaCards human disease database

More...
MalaCardsi
ALKBH8
MIMi618504 phenotype

Open Targets

More...
OpenTargetsi
ENSG00000137760

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA143485296

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q96BT7 Tbio

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
ALKBH8

Domain mapping of disease mutations (DMDM)

More...
DMDMi
189027650

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003371251 – 664Alkylated DNA repair protein alkB homolog 8Add BLAST664

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q96BT7

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
Q96BT7

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...
MassIVEi
Q96BT7

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q96BT7

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q96BT7

PeptideAtlas

More...
PeptideAtlasi
Q96BT7

PRoteomics IDEntifications database

More...
PRIDEi
Q96BT7

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
76112 [Q96BT7-1]
76113 [Q96BT7-2]
76114 [Q96BT7-3]
76115 [Q96BT7-4]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q96BT7

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q96BT7

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Widely expressed, with highest expression in spleen, followed by pancreas and lung.1 Publication

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated after DNA damage. Induction is mediated via ATM.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSG00000137760 Expressed in forebrain and 187 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q96BT7 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q96BT7 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
HPA038724
HPA038725
HPA061514

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with TRMT112.

4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
Q9UI305EBI-10825637,EBI-373326

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
124880, 3 interactors

Protein interaction database and analysis system

More...
IntActi
Q96BT7, 14 interactors

STRING: functional protein association networks

More...
STRINGi
9606.ENSP00000397673

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q96BT7 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1664
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q96BT7

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q96BT7

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini43 – 120RRMAdd BLAST78
Domaini220 – 337Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni227 – 229Alpha-ketoglutarate binding1 Publication3
Regioni411 – 664Methyltransferase domainAdd BLAST254

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the alkB family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1331 Eukaryota
KOG4176 Eukaryota
COG0500 LUCA
COG3145 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000158563

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_1173010_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q96BT7

KEGG Orthology (KO)

More...
KOi
K10770

Identification of Orthologs from Complete Genome Data

More...
OMAi
TNRTEFQ

Database of Orthologous Groups

More...
OrthoDBi
996085at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q96BT7

TreeFam database of animal gene trees

More...
TreeFami
TF316056

Family and domain databases

Conserved Domains Database

More...
CDDi
cd12431 RRM_ALKBH8, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.70.330, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR027450 AlkB-like
IPR015095 AlkB_hom8_N
IPR032863 ALKBH8
IPR034256 ALKBH8_RRM
IPR013216 Methyltransf_11
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR035979 RBD_domain_sf
IPR029063 SAM-dependent_MTases

The PANTHER Classification System

More...
PANTHERi
PTHR13069:SF28 PTHR13069:SF28, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13532 2OG-FeII_Oxy_2, 1 hit
PF09004 DUF1891, 1 hit
PF08241 Methyltransf_11, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53335 SSF53335, 1 hit
SSF54928 SSF54928, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96BT7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDSNHQSNYK LSKTEKKFLR KQIKAKHTLL RHEGIETVSY ATQSLVVANG
60 70 80 90 100
GLGNGVSRNQ LLPVLEKCGL VDALLMPPNK PYSFARYRTT EESKRAYVTL
110 120 130 140 150
NGKEVVDDLG QKITLYLNFV EKVQWKELRP QALPPGLMVV EEIISSEEEK
160 170 180 190 200
MLLESVDWTE DTDNQNSQKS LKHRRVKHFG YEFHYENNNV DKDKPLSGGL
210 220 230 240 250
PDICESFLEK WLRKGYIKHK PDQMTINQYE PGQGIPAHID THSAFEDEIV
260 270 280 290 300
SLSLGSEIVM DFKHPDGIAV PVMLPRRSLL VMTGESRYLW THGITCRKFD
310 320 330 340 350
TVQASESLKS GIITSDVGDL TLSKRGLRTS FTFRKVRQTP CNCSYPLVCD
360 370 380 390 400
SQRKETPPSF PESDKEASRL EQEYVHQVYE EIAGHFSSTR HTPWPHIVEF
410 420 430 440 450
LKALPSGSIV ADIGCGNGKY LGINKELYMI GCDRSQNLVD ICRERQFQAF
460 470 480 490 500
VCDALAVPVR SGSCDACISI AVIHHFATAE RRVAALQEIV RLLRPGGKAL
510 520 530 540 550
IYVWAMEQEY NKQKSKYLRG NRNSQGKKEE MNSDTSVQRS LVEQMRDMGS
560 570 580 590 600
RDSASSVPRI NDSQEGGCNS RQVSNSKLPV HVNRTSFYSQ DVLVPWHLKG
610 620 630 640 650
NPDKGKPVEP FGPIGSQDPS PVFHRYYHVF REGELEGACR TVSDVRILQS
660
YYDQGNWCVI LQKA
Length:664
Mass (Da):75,208
Last modified:May 20, 2008 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4BE595D6757C2A43
GO
Isoform 2 (identifier: Q96BT7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     234-238: GIPAH → DCHGF
     239-664: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »
Length:238
Mass (Da):27,440
Checksum:i24EF6A3A66DCC2F7
GO
Isoform 3 (identifier: Q96BT7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-224: GYIKHKPDQM → AEKNLEVGIH
     225-664: Missing.

Show »
Length:224
Mass (Da):25,742
Checksum:i6FD66E832509EE87
GO
Isoform 4 (identifier: Q96BT7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFAM

Note: May be due to competing donor splice site.Curated
Show »
Length:667
Mass (Da):75,558
Checksum:iBF6DB408A2B1B39C
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JQN2C9JQN2_HUMAN
Alkylated DNA repair protein alkB h...
ALKBH8
144Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti210K → R in BAC04566 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_083119554 – 664Missing in MRT71; missing tRNA wobble uridine modification. 1 PublicationAdd BLAST111

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0391591M → MFAM in isoform 4. 1 Publication1
Alternative sequenceiVSP_033925215 – 224GYIKHKPDQM → AEKNLEVGIH in isoform 3. 1 Publication10
Alternative sequenceiVSP_033926225 – 664Missing in isoform 3. 1 PublicationAdd BLAST440
Alternative sequenceiVSP_033927234 – 238GIPAH → DCHGF in isoform 2. 1 Publication5
Alternative sequenceiVSP_033928239 – 664Missing in isoform 2. 1 PublicationAdd BLAST426

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB218768 mRNA Translation: BAG16270.1
AK095523 mRNA Translation: BAC04566.1
AK293603 mRNA Translation: BAG57067.1
AK304413 mRNA Translation: BAG65244.1
AP001823 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67089.1
CH471065 Genomic DNA Translation: EAW67090.1
BC015183 mRNA Translation: AAH15183.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS73376.1 [Q96BT7-4]
CCDS8337.2 [Q96BT7-1]

NCBI Reference Sequences

More...
RefSeqi
NP_001287939.1, NM_001301010.1 [Q96BT7-4]
NP_620130.2, NM_138775.2 [Q96BT7-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000260318; ENSP00000260318; ENSG00000137760 [Q96BT7-2]
ENST00000389568; ENSP00000374219; ENSG00000137760 [Q96BT7-1]
ENST00000417449; ENSP00000397673; ENSG00000137760 [Q96BT7-4]
ENST00000428149; ENSP00000415885; ENSG00000137760 [Q96BT7-1]
ENST00000429370; ENSP00000391225; ENSG00000137760 [Q96BT7-3]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
91801

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:91801

UCSC genome browser

More...
UCSCi
uc009yxp.4 human [Q96BT7-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB218768 mRNA Translation: BAG16270.1
AK095523 mRNA Translation: BAC04566.1
AK293603 mRNA Translation: BAG57067.1
AK304413 mRNA Translation: BAG65244.1
AP001823 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67089.1
CH471065 Genomic DNA Translation: EAW67090.1
BC015183 mRNA Translation: AAH15183.1
CCDSiCCDS73376.1 [Q96BT7-4]
CCDS8337.2 [Q96BT7-1]
RefSeqiNP_001287939.1, NM_001301010.1 [Q96BT7-4]
NP_620130.2, NM_138775.2 [Q96BT7-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQ2NMR-A25-125[»]
3THPX-ray3.20A25-355[»]
3THTX-ray3.01A/B/C/D25-355[»]
SMRiQ96BT7
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi124880, 3 interactors
IntActiQ96BT7, 14 interactors
STRINGi9606.ENSP00000397673

PTM databases

iPTMnetiQ96BT7
PhosphoSitePlusiQ96BT7

Polymorphism and mutation databases

BioMutaiALKBH8
DMDMi189027650

Proteomic databases

EPDiQ96BT7
jPOSTiQ96BT7
MassIVEiQ96BT7
MaxQBiQ96BT7
PaxDbiQ96BT7
PeptideAtlasiQ96BT7
PRIDEiQ96BT7
ProteomicsDBi76112 [Q96BT7-1]
76113 [Q96BT7-2]
76114 [Q96BT7-3]
76115 [Q96BT7-4]

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
91801

Genome annotation databases

EnsembliENST00000260318; ENSP00000260318; ENSG00000137760 [Q96BT7-2]
ENST00000389568; ENSP00000374219; ENSG00000137760 [Q96BT7-1]
ENST00000417449; ENSP00000397673; ENSG00000137760 [Q96BT7-4]
ENST00000428149; ENSP00000415885; ENSG00000137760 [Q96BT7-1]
ENST00000429370; ENSP00000391225; ENSG00000137760 [Q96BT7-3]
GeneIDi91801
KEGGihsa:91801
UCSCiuc009yxp.4 human [Q96BT7-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
91801
DisGeNETi91801

GeneCards: human genes, protein and diseases

More...
GeneCardsi
ALKBH8
HGNCiHGNC:25189 ALKBH8
HPAiHPA038724
HPA038725
HPA061514
MalaCardsiALKBH8
MIMi613306 gene
618504 phenotype
neXtProtiNX_Q96BT7
OpenTargetsiENSG00000137760
PharmGKBiPA143485296

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1331 Eukaryota
KOG4176 Eukaryota
COG0500 LUCA
COG3145 LUCA
GeneTreeiENSGT00940000158563
HOGENOMiCLU_1173010_0_0_1
InParanoidiQ96BT7
KOiK10770
OMAiTNRTEFQ
OrthoDBi996085at2759
PhylomeDBiQ96BT7
TreeFamiTF316056

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000137760-MONOMER
BRENDAi2.1.1.229 2681
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
ALKBH8 human
EvolutionaryTraceiQ96BT7

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
91801
PharosiQ96BT7 Tbio

Protein Ontology

More...
PROi
PR:Q96BT7
RNActiQ96BT7 protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000137760 Expressed in forebrain and 187 other tissues
ExpressionAtlasiQ96BT7 baseline and differential
GenevisibleiQ96BT7 HS

Family and domain databases

CDDicd12431 RRM_ALKBH8, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR027450 AlkB-like
IPR015095 AlkB_hom8_N
IPR032863 ALKBH8
IPR034256 ALKBH8_RRM
IPR013216 Methyltransf_11
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR035979 RBD_domain_sf
IPR029063 SAM-dependent_MTases
PANTHERiPTHR13069:SF28 PTHR13069:SF28, 1 hit
PfamiView protein in Pfam
PF13532 2OG-FeII_Oxy_2, 1 hit
PF09004 DUF1891, 1 hit
PF08241 Methyltransf_11, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiALKB8_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q96BT7
Secondary accession number(s): B1Q2M0, B4DEF6, Q8N989
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: February 26, 2020
This is version 160 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again