Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Centromere protein T

Gene

CENPT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein T
Short name:
CENP-T
Alternative name(s):
Interphase centromere complex protein 22
Gene namesi
Name:CENPT
Synonyms:C16orf56, ICEN22
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000102901.12
HGNCiHGNC:25787 CENPT
MIMi611510 gene
neXtProtiNX_Q96BT3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000102901
PharmGKBiPA142672263

Polymorphism and mutation databases

BioMutaiCENPT
DMDMi74760746

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495141 – 561Centromere protein TAdd BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei47PhosphoserineCombined sources1 Publication1
Modified residuei85PhosphothreonineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei345PhosphoserineBy similarity1
Modified residuei356PhosphoserineBy similarity1
Modified residuei373PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1
Modified residuei386PhosphoserineCombined sources1
Modified residuei397PhosphoserineCombined sources1

Post-translational modificationi

Dynamically phosphorylated at Ser-47 and probably also other sites during the cell cycle. Phosphorylated at Ser-47 during G2 phase, metaphase and anaphase, but not during telophase or G1 phase.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96BT3
MaxQBiQ96BT3
PaxDbiQ96BT3
PeptideAtlasiQ96BT3
PRIDEiQ96BT3
ProteomicsDBi76109
76110 [Q96BT3-2]
76111 [Q96BT3-3]

PTM databases

iPTMnetiQ96BT3
PhosphoSitePlusiQ96BT3

Expressioni

Gene expression databases

BgeeiENSG00000102901 Expressed in 128 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_CENPT
ExpressionAtlasiQ96BT3 baseline and differential
GenevisibleiQ96BT3 HS

Organism-specific databases

HPAiHPA041220
HPA058036

Interactioni

Subunit structurei

Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU (PubMed:16622419, PubMed:19533040, PubMed:19412974). Identified in a centromeric complex containing histones H2A, H2B, H3 and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:19412974, PubMed:21695110, PubMed:27499292). Interacts (via N-terminus) with the NDC80 complex (Probable). Heterodimer with CENPW; this dimer coassembles with CENPS-CENPX heterodimers at centromeres to form the tetrameric CENP-T-W-S-X complex (PubMed:19533040, PubMed:19070575, PubMed:21529714, PubMed:21695110, PubMed:22304917).1 Publication8 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123143, 16 interactors
CORUMiQ96BT3
IntActiQ96BT3, 13 interactors
MINTiQ96BT3
STRINGi9606.ENSP00000219172

Structurei

3D structure databases

ProteinModelPortaliQ96BT3
SMRiQ96BT3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 421Flexible stalk domainBy similarityAdd BLAST329

Domaini

The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold.By similarity

Sequence similaritiesi

Belongs to the CENP-T/CNN1 family.Curated

Phylogenomic databases

eggNOGiENOG410IFJ0 Eukaryota
ENOG4111ZWH LUCA
GeneTreeiENSGT00390000003044
HOGENOMiHOG000111545
HOVERGENiHBG081089
InParanoidiQ96BT3
KOiK11512
OMAiFSFYAKM
OrthoDBiEOG091G05OQ
PhylomeDBiQ96BT3
TreeFamiTF332946

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR028255 CENP-T
IPR035425 CENP-T/H4_C
IPR032373 CENP-T_N
IPR009072 Histone-fold
PANTHERiPTHR10484:SF80 PTHR10484:SF80, 1 hit
PfamiView protein in Pfam
PF15511 CENP-T_C, 1 hit
PF16171 CENP-T_N, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 9 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q96BT3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADHNPDSDS TPRTLLRRVL DTADPRTPRR PRSARAGARR ALLETASPRK
60 70 80 90 100
LSGQTRTIAR GRSHGARSVG RSAHIQASGH LEEQTPRTLL KNILLTAPES
110 120 130 140 150
SILMPESVVK PVPAPQAVQP SRQESSCGSL ELQLPELEPP TTLAPGLLAP
160 170 180 190 200
GRRKQRLRLS VFQQGVDQGL SLSQEPQGNA DASSLTRSLN LTFATPLQPQ
210 220 230 240 250
SVQRPGLARR PPARRAVDVG AFLRDLRDTS LAPPNIVLED TQPFSQPMVG
260 270 280 290 300
SPNVYHSLPC TPHTGAEDAE QAAGRKTQSS GPGLQKNSPG KPAQFLAGEA
310 320 330 340 350
EEVNAFALGF LSTSSGVSGE DEVEPLHDGV EEAEKKMEEE GVSVSEMEAT
360 370 380 390 400
GAQGPSRVEE AEGHTEVTEA EGSQGTAEAD GPGASSGDED ASGRAASPES
410 420 430 440 450
ASSTPESLQA RRHHQFLEPA PAPGAAVLSS EPAEPLLVRH PPRPRTTGPR
460 470 480 490 500
PRQDPHKAGL SHYVKLFSFY AKMPMERKAL EMVEKCLDKY FQHLCDDLEV
510 520 530 540 550
FAAHAGRKTV KPEDLELLMR RQGLVTDQVS LHVLVERHLP LEYRQLLIPC
560
AYSGNSVFPA Q
Length:561
Mass (Da):60,423
Last modified:March 1, 2004 - v2
Checksum:i295848C52AAF7DF1
GO
Isoform 2 (identifier: Q96BT3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     289-299: PGKPAQFLAGE → ECVALVAWSQI
     300-561: Missing.

Note: No experimental confirmation available.
Show »
Length:299
Mass (Da):32,170
Checksum:i437895CBF0AD00DC
GO
Isoform 3 (identifier: Q96BT3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-151: SVGRSAHIQA...TLAPGLLAPG → VSTQPTDPKG...NCPRIFHPDA
     152-561: Missing.

Note: No experimental confirmation available.
Show »
Length:151
Mass (Da):16,434
Checksum:i4A936224C3E78049
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BTR4H3BTR4_HUMAN
Centromere protein T
CENPT
506Annotation score:
I3L4F8I3L4F8_HUMAN
Centromere protein T
CENPT
163Annotation score:
H3BUW2H3BUW2_HUMAN
Centromere protein T
CENPT
277Annotation score:
H3BQJ0H3BQJ0_HUMAN
Centromere protein T
CENPT
114Annotation score:
H3BQS6H3BQS6_HUMAN
Centromere protein T
CENPT
73Annotation score:
H3BTS2H3BTS2_HUMAN
Centromere protein T
CENPT
101Annotation score:
H3BN05H3BN05_HUMAN
Centromere protein T
CENPT
79Annotation score:
H3BQ71H3BQ71_HUMAN
Centromere protein T
CENPT
48Annotation score:
H3BMP6H3BMP6_HUMAN
Centromere protein T
CENPT
64Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027421115P → L. Corresponds to variant dbSNP:rs12102580Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02045568 – 151SVGRS…LLAPG → VSTQPTDPKGPWLPRGGGLR SSSALEPTLRKSQGRRTDWL LGASPIVCWQIGPYSGQWAL GGTDTSDAAEEHPTNCPRIF HPDA in isoform 3. 1 PublicationAdd BLAST84
Alternative sequenceiVSP_020456152 – 561Missing in isoform 3. 1 PublicationAdd BLAST410
Alternative sequenceiVSP_020457289 – 299PGKPAQFLAGE → ECVALVAWSQI in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_020458300 – 561Missing in isoform 2. 1 PublicationAdd BLAST262

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023173 mRNA Translation: BAB14445.1
AK055237 mRNA Translation: BAB70884.1
BC007642 mRNA Translation: AAH07642.1
BC007864 mRNA Translation: AAH07864.2
BC015202 mRNA Translation: AAH15202.2
CCDSiCCDS42182.1 [Q96BT3-1]
RefSeqiNP_079358.3, NM_025082.3 [Q96BT3-1]
UniGeneiHs.288382

Genome annotation databases

EnsembliENST00000440851; ENSP00000400140; ENSG00000102901 [Q96BT3-1]
ENST00000562787; ENSP00000457810; ENSG00000102901 [Q96BT3-1]
GeneIDi80152
KEGGihsa:80152
UCSCiuc002eun.4 human [Q96BT3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023173 mRNA Translation: BAB14445.1
AK055237 mRNA Translation: BAB70884.1
BC007642 mRNA Translation: AAH07642.1
BC007864 mRNA Translation: AAH07864.2
BC015202 mRNA Translation: AAH15202.2
CCDSiCCDS42182.1 [Q96BT3-1]
RefSeqiNP_079358.3, NM_025082.3 [Q96BT3-1]
UniGeneiHs.288382

3D structure databases

ProteinModelPortaliQ96BT3
SMRiQ96BT3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123143, 16 interactors
CORUMiQ96BT3
IntActiQ96BT3, 13 interactors
MINTiQ96BT3
STRINGi9606.ENSP00000219172

PTM databases

iPTMnetiQ96BT3
PhosphoSitePlusiQ96BT3

Polymorphism and mutation databases

BioMutaiCENPT
DMDMi74760746

Proteomic databases

EPDiQ96BT3
MaxQBiQ96BT3
PaxDbiQ96BT3
PeptideAtlasiQ96BT3
PRIDEiQ96BT3
ProteomicsDBi76109
76110 [Q96BT3-2]
76111 [Q96BT3-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000440851; ENSP00000400140; ENSG00000102901 [Q96BT3-1]
ENST00000562787; ENSP00000457810; ENSG00000102901 [Q96BT3-1]
GeneIDi80152
KEGGihsa:80152
UCSCiuc002eun.4 human [Q96BT3-1]

Organism-specific databases

CTDi80152
EuPathDBiHostDB:ENSG00000102901.12
GeneCardsiCENPT
HGNCiHGNC:25787 CENPT
HPAiHPA041220
HPA058036
MIMi611510 gene
neXtProtiNX_Q96BT3
OpenTargetsiENSG00000102901
PharmGKBiPA142672263
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFJ0 Eukaryota
ENOG4111ZWH LUCA
GeneTreeiENSGT00390000003044
HOGENOMiHOG000111545
HOVERGENiHBG081089
InParanoidiQ96BT3
KOiK11512
OMAiFSFYAKM
OrthoDBiEOG091G05OQ
PhylomeDBiQ96BT3
TreeFamiTF332946

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-68877 Mitotic Prometaphase

Miscellaneous databases

ChiTaRSiCENPT human
GeneWikiiCENPT
GenomeRNAii80152
PROiPR:Q96BT3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102901 Expressed in 128 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_CENPT
ExpressionAtlasiQ96BT3 baseline and differential
GenevisibleiQ96BT3 HS

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR028255 CENP-T
IPR035425 CENP-T/H4_C
IPR032373 CENP-T_N
IPR009072 Histone-fold
PANTHERiPTHR10484:SF80 PTHR10484:SF80, 1 hit
PfamiView protein in Pfam
PF15511 CENP-T_C, 1 hit
PF16171 CENP-T_N, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCENPT_HUMAN
AccessioniPrimary (citable) accession number: Q96BT3
Secondary accession number(s): Q96I29
, Q96IC6, Q96NK9, Q9H901
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2004
Last modified: September 12, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again