UniProtKB - Q96BS2 (CHP3_HUMAN)
Protein
Calcineurin B homologous protein 3
Gene
TESC
Organism
Homo sapiens (Human)
Status
Functioni
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Promotes the maturation, transport, cell surface stability and exchange activity of SLC9A1/NHE1 at the plasma membrane. Promotes the induction of hematopoietic stem cell differentiation toward megakaryocytic lineage. Essential for the coupling of ERK cascade activation with the expression of ETS family genes in megakaryocytic differentiation. Also involved in granulocytic differentiation in a ERK-dependent manner. Inhibits the phosphatase activity of calcineurin.3 Publications
Caution
Although PubMed:12809501 reports that TESC results in a decrease in transporter activity of human SLC9A1, studies with rat SLC9A1 show that TESC-binding results in the maturation and accumulation of SLC9A1 at the cell surface.1 Publication
The interacting region of SLC9A1/NHE1 with CHP3 is conflicting: Interaction with SLC9A1/NHE1 has been reported via residues 503-545, the juxtamembrane region of the cytoplasmic C-terminus (PubMed:11696366, PubMed:18321853, PubMed:30287853). However, another publication has reported interaction with SLC9A1/NHE1 via residues 633-815, the region of the cytoplasmic C-terminus more distal to the membrane (PubMed:12809501).4 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 123 – 134 | PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- phosphatase inhibitor activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- protein kinase inhibitor activity Source: UniProtKB-KW
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- cellular response to retinoic acid Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- positive regulation of gene expression Source: UniProtKB
- positive regulation of granulocyte differentiation Source: UniProtKB
- positive regulation of megakaryocyte differentiation Source: UniProtKB
- positive regulation of sodium:proton antiporter activity Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- protein localization to plasma membrane Source: UniProtKB
- protein maturation Source: UniProtKB
- protein stabilization Source: UniProtKB
- protein transport Source: UniProtKB-KW
- regulation of cell adhesion mediated by integrin Source: UniProtKB
Keywordsi
Molecular function | Protein kinase inhibitor |
Biological process | Differentiation, Protein transport, Transport |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q96BS2 |
Names & Taxonomyi
Protein namesi | Recommended name: Calcineurin B homologous protein 3Alternative name(s): Tescalcin Short name: TSC |
Gene namesi | Name:TESC Synonyms:CHP3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26065, TESC |
MIMi | 611585, gene |
neXtProti | NX_Q96BS2 |
VEuPathDBi | HostDB:ENSG00000088992.17 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
- ruffle membrane By similarity
Nucleus
- Nucleus 1 Publication
Other locations
- Cytoplasm 1 Publication
- Membrane Curated; Lipid-anchor Curated
- lamellipodium 1 Publication
Note: Colocalizes with SLC9A1 at the plasma membrane.1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
- ruffle membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB
- lamellipodium Source: UniProtKB
- ruffle Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 54997 |
OpenTargetsi | ENSG00000088992 |
PharmGKBi | PA143485630 |
Miscellaneous databases
Pharosi | Q96BS2, Tbio |
Genetic variation databases
BioMutai | TESC |
DMDMi | 284018160 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000073859 | 2 – 214 | Calcineurin B homologous protein 3Add BLAST | 213 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine3 Publications | 1 |
Keywords - PTMi
Disulfide bond, Lipoprotein, MyristateProteomic databases
EPDi | Q96BS2 |
jPOSTi | Q96BS2 |
MassIVEi | Q96BS2 |
MaxQBi | Q96BS2 |
PaxDbi | Q96BS2 |
PeptideAtlasi | Q96BS2 |
PRIDEi | Q96BS2 |
ProteomicsDBi | 25797 76106 [Q96BS2-1] 76107 [Q96BS2-2] |
PTM databases
iPTMneti | Q96BS2 |
PhosphoSitePlusi | Q96BS2 |
SwissPalmi | Q96BS2 |
Expressioni
Tissue specificityi
Expressed in mature megakaryocytes and polymorphonuclear granulocytes (at protein level). Abundantly expressed in heart. Also expressed at a lower level in adult testis and salivary gland, and in the placenta.2 Publications
Developmental stagei
Strongly up-regulated in K562 cells treated by PMA to promote megakaryocytic differentiation, but not when treated by DMSO to promote granulocytic differentiation or by hemin to promote erythroid differentiation (at protein level).1 Publication
Inductioni
Up-regulated during granulocytic differentiation in a ERK-dependent manner (is mediated by activation of ERK) (at protein level). Up-regulated during the differentiation and maturation of primary megakaryocytes. Down-regulated during monocytic-macrophage differentiation in a ERK-dependent manner.2 Publications
Gene expression databases
Bgeei | ENSG00000088992, Expressed in saliva-secreting gland and 200 other tissues |
ExpressionAtlasi | Q96BS2, baseline and differential |
Genevisiblei | Q96BS2, HS |
Organism-specific databases
HPAi | ENSG00000088992, Tissue enhanced (blood, salivary gland) |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ96BS2
With | #Exp. | IntAct |
---|---|---|
HMG20A [Q9NP66] | 2 | EBI-740653,EBI-740641 |
SLC9A1 [P19634] | 4 | EBI-740653,EBI-743635 |
WBP11 [Q9Y2W2] | 3 | EBI-740653,EBI-714455 |
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 120330, 8 interactors |
IntActi | Q96BS2, 20 interactors |
STRINGi | 9606.ENSP00000334785 |
Miscellaneous databases
RNActi | Q96BS2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 110 – 145 | EF-handPROSITE-ProRule annotationAdd BLAST | 36 |
Domaini
Binds calcium via its EF-hands. Calcium-binding mediates a conformational change. Can also bind magnesium (By similarity).By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0034, Eukaryota |
GeneTreei | ENSGT00940000155845 |
HOGENOMi | CLU_112076_0_0_1 |
InParanoidi | Q96BS2 |
OMAi | IETRMHI |
OrthoDBi | 1271942at2759 |
PhylomeDBi | Q96BS2 |
TreeFami | TF354284 |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
SMARTi | View protein in SMART SM00054, EFh, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96BS2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGAAHSASEE VRELEGKTGF SSDQIEQLHR RFKQLSGDQP TIRKENFNNV
60 70 80 90 100
PDLELNPIRS KIVRAFFDNR NLRKGPSGLA DEINFEDFLT IMSYFRPIDT
110 120 130 140 150
TMDEEQVELS RKEKLRFLFH MYDSDSDGRI TLEEYRNVVE ELLSGNPHIE
160 170 180 190 200
KESARSIADG AMMEAASVCM GQMEPDQVYE GITFEDFLKI WQGIDIETKM
210
HVRFLNMETM ALCH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PQ58 | E9PQ58_HUMAN | Calcineurin B homologous protein 3 | TESC | 135 | Annotation score: | ||
H0YIX4 | H0YIX4_HUMAN | Calcineurin B homologous protein 3 | TESC | 81 | Annotation score: |
Sequence cautioni
The sequence BAA91288 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence EAW98101 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 209 – 211 | TMA → NMG in BQ949772 (PubMed:15489334).Curated | 3 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045287 | 43 – 69 | Missing in isoform 3. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_035518 | 139 – 163 | VEELL…DGAMM → KWSRSCCRETLTSRRSPLAP SPTGP in isoform 2. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_035519 | 164 – 214 | Missing in isoform 2. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF443207 mRNA Translation: AAL35615.1 AK000614 mRNA Translation: BAA91288.1 Different initiation. AC026368 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW98101.1 Different initiation. BC015221 mRNA Translation: AAH15221.1 BQ949772 mRNA No translation available. |
CCDSi | CCDS53835.1 [Q96BS2-3] CCDS9183.3 [Q96BS2-1] |
RefSeqi | NP_001161797.1, NM_001168325.1 [Q96BS2-3] NP_060369.3, NM_017899.3 [Q96BS2-1] XP_016875022.1, XM_017019533.1 |
Genome annotation databases
Ensembli | ENST00000335209; ENSP00000334785; ENSG00000088992 [Q96BS2-1] ENST00000470612; ENSP00000432716; ENSG00000088992 [Q96BS2-2] ENST00000541210; ENSP00000445689; ENSG00000088992 [Q96BS2-3] |
GeneIDi | 54997 |
KEGGi | hsa:54997 |
UCSCi | uc001twh.4, human [Q96BS2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF443207 mRNA Translation: AAL35615.1 AK000614 mRNA Translation: BAA91288.1 Different initiation. AC026368 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW98101.1 Different initiation. BC015221 mRNA Translation: AAH15221.1 BQ949772 mRNA No translation available. |
CCDSi | CCDS53835.1 [Q96BS2-3] CCDS9183.3 [Q96BS2-1] |
RefSeqi | NP_001161797.1, NM_001168325.1 [Q96BS2-3] NP_060369.3, NM_017899.3 [Q96BS2-1] XP_016875022.1, XM_017019533.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120330, 8 interactors |
IntActi | Q96BS2, 20 interactors |
STRINGi | 9606.ENSP00000334785 |
PTM databases
iPTMneti | Q96BS2 |
PhosphoSitePlusi | Q96BS2 |
SwissPalmi | Q96BS2 |
Genetic variation databases
BioMutai | TESC |
DMDMi | 284018160 |
Proteomic databases
EPDi | Q96BS2 |
jPOSTi | Q96BS2 |
MassIVEi | Q96BS2 |
MaxQBi | Q96BS2 |
PaxDbi | Q96BS2 |
PeptideAtlasi | Q96BS2 |
PRIDEi | Q96BS2 |
ProteomicsDBi | 25797 76106 [Q96BS2-1] 76107 [Q96BS2-2] |
Protocols and materials databases
Antibodypediai | 45432, 168 antibodies |
DNASUi | 54997 |
Genome annotation databases
Ensembli | ENST00000335209; ENSP00000334785; ENSG00000088992 [Q96BS2-1] ENST00000470612; ENSP00000432716; ENSG00000088992 [Q96BS2-2] ENST00000541210; ENSP00000445689; ENSG00000088992 [Q96BS2-3] |
GeneIDi | 54997 |
KEGGi | hsa:54997 |
UCSCi | uc001twh.4, human [Q96BS2-1] |
Organism-specific databases
CTDi | 54997 |
DisGeNETi | 54997 |
GeneCardsi | TESC |
HGNCi | HGNC:26065, TESC |
HPAi | ENSG00000088992, Tissue enhanced (blood, salivary gland) |
MIMi | 611585, gene |
neXtProti | NX_Q96BS2 |
OpenTargetsi | ENSG00000088992 |
PharmGKBi | PA143485630 |
VEuPathDBi | HostDB:ENSG00000088992.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0034, Eukaryota |
GeneTreei | ENSGT00940000155845 |
HOGENOMi | CLU_112076_0_0_1 |
InParanoidi | Q96BS2 |
OMAi | IETRMHI |
OrthoDBi | 1271942at2759 |
PhylomeDBi | Q96BS2 |
TreeFami | TF354284 |
Enzyme and pathway databases
PathwayCommonsi | Q96BS2 |
Miscellaneous databases
BioGRID-ORCSi | 54997, 6 hits in 874 CRISPR screens |
ChiTaRSi | TESC, human |
GenomeRNAii | 54997 |
Pharosi | Q96BS2, Tbio |
PROi | PR:Q96BS2 |
RNActi | Q96BS2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000088992, Expressed in saliva-secreting gland and 200 other tissues |
ExpressionAtlasi | Q96BS2, baseline and differential |
Genevisiblei | Q96BS2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
SMARTi | View protein in SMART SM00054, EFh, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHP3_HUMAN | |
Accessioni | Q96BS2Primary (citable) accession number: Q96BS2 Secondary accession number(s): F5H1Y5, Q9NWT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | January 19, 2010 | |
Last modified: | February 10, 2021 | |
This is version 157 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families