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Protein

E3 ubiquitin-protein ligase RNF25

Gene

RNF25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri135 – 202RING-typePROSITE-ProRule annotationAdd BLAST68

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NF-kappaB binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF25 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 25
RING-type E3 ubiquitin transferase RNF25Curated
Gene namesi
Name:RNF25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163481.7
HGNCiHGNC:14662 RNF25
MIMi616014 gene
neXtProtiNX_Q96BH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi159C → S: Reduced activation of NF-kappa-B. 1 Publication1
Mutagenesisi161C → S: Strongly reduced activation of NF-kappa-B. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000163481
PharmGKBiPA34429

Polymorphism and mutation databases

BioMutaiRNF25
DMDMi21362899

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560661 – 459E3 ubiquitin-protein ligase RNF25Add BLAST459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei450PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96BH1
MaxQBiQ96BH1
PaxDbiQ96BH1
PeptideAtlasiQ96BH1
PRIDEiQ96BH1
ProteomicsDBi76075

PTM databases

iPTMnetiQ96BH1
PhosphoSitePlusiQ96BH1

Expressioni

Gene expression databases

BgeeiENSG00000163481 Expressed in 178 organ(s), highest expression level in adenohypophysis
CleanExiHS_RNF25
ExpressionAtlasiQ96BH1 baseline and differential
GenevisibleiQ96BH1 HS

Organism-specific databases

HPAiHPA036420
HPA036421

Interactioni

Subunit structurei

Interacts with UBE2D2, and may also interact with UBE2E1 and UBE2E3. Interacts with NKD2 (By similarity). Interacts with RELA.By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi122133, 26 interactors
DIPiDIP-29063N
IntActiQ96BH1, 13 interactors
STRINGi9606.ENSP00000295704

Structurei

Secondary structure

1459
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96BH1
SMRiQ96BH1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96BH1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 128RWDPROSITE-ProRule annotationAdd BLAST111

Domaini

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri135 – 202RING-typePROSITE-ProRule annotationAdd BLAST68

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4445 Eukaryota
ENOG4111H5K LUCA
GeneTreeiENSGT00390000001557
HOGENOMiHOG000012992
HOVERGENiHBG055249
InParanoidiQ96BH1
KOiK10640
OMAiNGRSSPW
OrthoDBiEOG091G0IBN
PhylomeDBiQ96BH1
TreeFamiTF324097

Family and domain databases

Gene3Di3.10.110.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039133 RNF25
IPR006575 RWD-domain
IPR016135 UBQ-conjugating_enzyme/RWD
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR13198 PTHR13198, 1 hit
PfamiView protein in Pfam
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q96BH1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAASASAAAG EEDWVLPSEV EVLESIYLDE LQVIKGNGRT SPWEIYITLH
60 70 80 90 100
PATAEDQDSQ YVCFTLVLQV PAEYPHEVPQ ISIRNPRGLS DEQIHTILQV
110 120 130 140 150
LGHVAKAGLG TAMLYELIEK GKEILTDNNI PHGQCVICLY GFQEKEAFTK
160 170 180 190 200
TPCYHYFHCH CLARYIQHME QELKAQGQEQ EQERQHATTK QKAVGVQCPV
210 220 230 240 250
CREPLVYDLA SLKAAPEPQQ PMELYQPSAE SLRQQEERKR LYQRQQERGG
260 270 280 290 300
IIDLEAERNR YFISLQQPPA PAEPESAVDV SKGSQPPSTL AAELSTSPAV
310 320 330 340 350
QSTLPPPLPV ATQHICEKIP GTRSNQQRLG ETQKAMLDPP KPSRGPWRQP
360 370 380 390 400
ERRHPKGGEC HAPKGTRDTQ ELPPPEGPLK EPMDLKPEPH SQGVEGPPQE
410 420 430 440 450
KGPGSWQGPP PRRTRDCVRW ERSKGRTPGS SYPRLPRGQG AYRPGTRRES

LGLESKDGS
Length:459
Mass (Da):51,219
Last modified:December 1, 2001 - v1
Checksum:iCE7C66EF2E988917
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C219H7C219_HUMAN
E3 ubiquitin-protein ligase RNF25
RNF25
158Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186H → R in BAD96245 (Ref. 2) Curated1
Sequence conflicti346P → S in BAD96245 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023968 mRNA Translation: BAB14743.1
AK289501 mRNA Translation: BAF82190.1
AK222525 mRNA Translation: BAD96245.1
AC009974 Genomic DNA No translation available.
CH471063 Genomic DNA Translation: EAW70645.1
BC015612 mRNA Translation: AAH15612.1
CCDSiCCDS2420.1
RefSeqiNP_071898.2, NM_022453.2
UniGeneiHs.471403

Genome annotation databases

EnsembliENST00000295704; ENSP00000295704; ENSG00000163481
GeneIDi64320
KEGGihsa:64320
UCSCiuc002vit.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023968 mRNA Translation: BAB14743.1
AK289501 mRNA Translation: BAF82190.1
AK222525 mRNA Translation: BAD96245.1
AC009974 Genomic DNA No translation available.
CH471063 Genomic DNA Translation: EAW70645.1
BC015612 mRNA Translation: AAH15612.1
CCDSiCCDS2420.1
RefSeqiNP_071898.2, NM_022453.2
UniGeneiHs.471403

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DAYNMR-A11-125[»]
2DMFNMR-A11-134[»]
5D1KX-ray1.78B126-258[»]
5D1LX-ray1.62B126-258[»]
5D1MX-ray1.58B126-258[»]
ProteinModelPortaliQ96BH1
SMRiQ96BH1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122133, 26 interactors
DIPiDIP-29063N
IntActiQ96BH1, 13 interactors
STRINGi9606.ENSP00000295704

PTM databases

iPTMnetiQ96BH1
PhosphoSitePlusiQ96BH1

Polymorphism and mutation databases

BioMutaiRNF25
DMDMi21362899

Proteomic databases

EPDiQ96BH1
MaxQBiQ96BH1
PaxDbiQ96BH1
PeptideAtlasiQ96BH1
PRIDEiQ96BH1
ProteomicsDBi76075

Protocols and materials databases

DNASUi64320
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295704; ENSP00000295704; ENSG00000163481
GeneIDi64320
KEGGihsa:64320
UCSCiuc002vit.4 human

Organism-specific databases

CTDi64320
EuPathDBiHostDB:ENSG00000163481.7
GeneCardsiRNF25
HGNCiHGNC:14662 RNF25
HPAiHPA036420
HPA036421
MIMi616014 gene
neXtProtiNX_Q96BH1
OpenTargetsiENSG00000163481
PharmGKBiPA34429
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4445 Eukaryota
ENOG4111H5K LUCA
GeneTreeiENSGT00390000001557
HOGENOMiHOG000012992
HOVERGENiHBG055249
InParanoidiQ96BH1
KOiK10640
OMAiNGRSSPW
OrthoDBiEOG091G0IBN
PhylomeDBiQ96BH1
TreeFamiTF324097

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

EvolutionaryTraceiQ96BH1
GeneWikiiRNF25
GenomeRNAii64320
PROiPR:Q96BH1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163481 Expressed in 178 organ(s), highest expression level in adenohypophysis
CleanExiHS_RNF25
ExpressionAtlasiQ96BH1 baseline and differential
GenevisibleiQ96BH1 HS

Family and domain databases

Gene3Di3.10.110.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039133 RNF25
IPR006575 RWD-domain
IPR016135 UBQ-conjugating_enzyme/RWD
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR13198 PTHR13198, 1 hit
PfamiView protein in Pfam
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNF25_HUMAN
AccessioniPrimary (citable) accession number: Q96BH1
Secondary accession number(s): A8K0D6, Q53HQ5, Q9H874
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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