UniProtKB - Q96BD8 (SKA1_HUMAN)
Spindle and kinetochore-associated protein 1
SKA1
Functioni
GO - Molecular functioni
- microtubule binding Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB
- chromosome segregation Source: UniProtKB
- mitotic cell cycle Source: UniProtKB
- regulation of microtubule polymerization or depolymerization Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q96BD8 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Spindle and kinetochore-associated protein 1 |
Gene namesi | Name:SKA1 Synonyms:C18orf24 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000154839.9 |
HGNCi | HGNC:28109, SKA1 |
MIMi | 616673, gene |
neXtProti | NX_Q96BD8 |
Subcellular locationi
Cytoskeleton
- spindle 1 Publication
Other locations
- kinetochore 1 Publication
Note: Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner (PubMed:17093495). Localizes to both the mitotic spindle and kinetochore-associated proteins (PubMed:17093495). Associates with kinetochores following microtubule attachment from prometaphase, through mid-anaphase and then vanishes in telophase (PubMed:17093495).1 Publication
Cytoskeleton
- microtubule cytoskeleton Source: HPA
- mitotic spindle Source: GO_Central
- spindle microtubule Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Other locations
- condensed chromosome outer kinetochore Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 155 | R → A: Abolishes microtubule binding; when associated with A-236 and A-245. 1 Publication | 1 | |
Mutagenesisi | 185 | S → D: Phosphomimetic mutant which strongly reduces microtubule binding; when associated with D-242. 1 Publication | 1 | |
Mutagenesisi | 236 | R → A: Abolishes microtubule binding; when associated with A-155 and A-245. 1 Publication | 1 | |
Mutagenesisi | 242 | S → D: Phosphomimetic mutant which strongly reduces microtubule binding; when associated with D-185. 1 Publication | 1 | |
Mutagenesisi | 245 | R → A: Abolishes microtubule binding; when associated with A-155 and A-236. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 220134 |
OpenTargetsi | ENSG00000154839 |
PharmGKBi | PA165429102 |
Miscellaneous databases
Pharosi | Q96BD8, Tbio |
Polymorphism and mutation databases
BioMutai | SKA1 |
DMDMi | 74731226 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000273155 | 2 – 255 | Spindle and kinetochore-associated protein 1Add BLAST | 254 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q96BD8 |
jPOSTi | Q96BD8 |
MassIVEi | Q96BD8 |
MaxQBi | Q96BD8 |
PaxDbi | Q96BD8 |
PeptideAtlasi | Q96BD8 |
PRIDEi | Q96BD8 |
ProteomicsDBi | 76069 [Q96BD8-1] 76070 [Q96BD8-2] |
PTM databases
iPTMneti | Q96BD8 |
PhosphoSitePlusi | Q96BD8 |
Expressioni
Gene expression databases
Bgeei | ENSG00000154839, Expressed in ventricular zone and 105 other tissues |
ExpressionAtlasi | Q96BD8, baseline and differential |
Genevisiblei | Q96BD8, HS |
Organism-specific databases
HPAi | ENSG00000154839, Tissue enhanced (lymphoid tissue, testis) |
Interactioni
Subunit structurei
Component of the SKA1 complex, composed of SKA1, SKA2 and SKA3 (PubMed:17093495).
Forms a heterodimer with SKA2; the heterodimer interacting with SKA3 (PubMed:17093495, PubMed:19289083, PubMed:23085020). The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers, each heterodimer interacting with a molecule of the SKA3 homodimer (PubMed:19289083). The core SKA1 complex associates with microtubules and forms oligomeric assemblies (PubMed:17093495, PubMed:19289083).
Interacts with microtubules; the interaction is direct (PubMed:19289083, PubMed:23085020).
Interacts with SKA2 (PubMed:19289083).
Interacts with SKA3 (PubMed:19289083, PubMed:23085020).
3 PublicationsBinary interactionsi
Hide detailsQ96BD8
GO - Molecular functioni
- microtubule binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 128632, 55 interactors |
ComplexPortali | CPX-5642, Kinetochore SKA complex |
IntActi | Q96BD8, 48 interactors |
MINTi | Q96BD8 |
STRINGi | 9606.ENSP00000285116 |
Miscellaneous databases
RNActi | Q96BD8, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96BD8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 132 – 255 | Microtubule binding1 PublicationAdd BLAST | 124 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 49 – 91 | Sequence analysisAdd BLAST | 43 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4832, Eukaryota |
GeneTreei | ENSGT00390000011654 |
HOGENOMi | CLU_096842_0_0_1 |
InParanoidi | Q96BD8 |
OMAi | IGHHRVI |
PhylomeDBi | Q96BD8 |
TreeFami | TF324442 |
Family and domain databases
Gene3Di | 1.10.10.1890, 1 hit |
InterProi | View protein in InterPro IPR009829, SKA1 IPR042031, SKA1-MBD |
PANTHERi | PTHR28573, PTHR28573, 1 hit |
Pfami | View protein in Pfam PF07160, SKA1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASSDLEQLC SHVNEKIGNI KKTLSLRNCG QEPTLKTVLN KIGDEIIVIN
60 70 80 90 100
ELLNKLELEI QYQEQTNNSL KELCESLEED YKDIEHLKEN VPSHLPQVTV
110 120 130 140 150
TQSCVKGSDL DPEEPIKVEE PEPVKKPPKE QRSIKEMPFI TCDEFNGVPS
160 170 180 190 200
YMKSRLTYNQ INDVIKEINK AVISKYKILH QPKKSMNSVT RNLYHRFIDE
210 220 230 240 250
ETKDTKGRYF IVEADIKEFT TLKADKKFHV LLNILRHCRR LSEVRGGGLT
RYVIT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EP61 | K7EP61_HUMAN | Spindle and kinetochore-associated ... | SKA1 | 104 | Annotation score: | ||
K7EPW0 | K7EPW0_HUMAN | Spindle and kinetochore-associated ... | SKA1 | 91 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_030091 | 91 | V → I1 PublicationCorresponds to variant dbSNP:rs6507992Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037250 | 104 – 150 | CVKGS…NGVPS → W in isoform 2. 1 PublicationAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK303464 mRNA Translation: BAG64506.1 AK313968 mRNA Translation: BAG36683.1 CH471096 Genomic DNA Translation: EAW62971.1 BC015706 mRNA Translation: AAH15706.1 |
CCDSi | CCDS11946.1 [Q96BD8-1] |
RefSeqi | NP_001034624.1, NM_001039535.2 [Q96BD8-1] NP_659497.1, NM_145060.3 [Q96BD8-1] |
Genome annotation databases
Ensembli | ENST00000285116; ENSP00000285116; ENSG00000154839 [Q96BD8-1] ENST00000398452; ENSP00000381470; ENSG00000154839 [Q96BD8-1] ENST00000417656; ENSP00000397222; ENSG00000154839 [Q96BD8-2] ENST00000571751; ENSP00000458992; ENSG00000262634 [Q96BD8-1] ENST00000616604; ENSP00000478259; ENSG00000262634 [Q96BD8-1] ENST00000633940; ENSP00000488815; ENSG00000262634 [Q96BD8-2] |
GeneIDi | 220134 |
KEGGi | hsa:220134 |
UCSCi | uc002let.4, human [Q96BD8-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK303464 mRNA Translation: BAG64506.1 AK313968 mRNA Translation: BAG36683.1 CH471096 Genomic DNA Translation: EAW62971.1 BC015706 mRNA Translation: AAH15706.1 |
CCDSi | CCDS11946.1 [Q96BD8-1] |
RefSeqi | NP_001034624.1, NM_001039535.2 [Q96BD8-1] NP_659497.1, NM_145060.3 [Q96BD8-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4AJ5 | X-ray | 3.32 | A/B/C/D/E/F/G/H/I/J | 1-91 | [»] | |
4C9Y | X-ray | 2.01 | A/B | 133-255 | [»] | |
4CA0 | X-ray | 2.26 | A/B | 133-255 | [»] | |
SMRi | Q96BD8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 128632, 55 interactors |
ComplexPortali | CPX-5642, Kinetochore SKA complex |
IntActi | Q96BD8, 48 interactors |
MINTi | Q96BD8 |
STRINGi | 9606.ENSP00000285116 |
PTM databases
iPTMneti | Q96BD8 |
PhosphoSitePlusi | Q96BD8 |
Polymorphism and mutation databases
BioMutai | SKA1 |
DMDMi | 74731226 |
Proteomic databases
EPDi | Q96BD8 |
jPOSTi | Q96BD8 |
MassIVEi | Q96BD8 |
MaxQBi | Q96BD8 |
PaxDbi | Q96BD8 |
PeptideAtlasi | Q96BD8 |
PRIDEi | Q96BD8 |
ProteomicsDBi | 76069 [Q96BD8-1] 76070 [Q96BD8-2] |
Protocols and materials databases
Antibodypediai | 22703, 92 antibodies |
DNASUi | 220134 |
Genome annotation databases
Ensembli | ENST00000285116; ENSP00000285116; ENSG00000154839 [Q96BD8-1] ENST00000398452; ENSP00000381470; ENSG00000154839 [Q96BD8-1] ENST00000417656; ENSP00000397222; ENSG00000154839 [Q96BD8-2] ENST00000571751; ENSP00000458992; ENSG00000262634 [Q96BD8-1] ENST00000616604; ENSP00000478259; ENSG00000262634 [Q96BD8-1] ENST00000633940; ENSP00000488815; ENSG00000262634 [Q96BD8-2] |
GeneIDi | 220134 |
KEGGi | hsa:220134 |
UCSCi | uc002let.4, human [Q96BD8-1] |
Organism-specific databases
CTDi | 220134 |
DisGeNETi | 220134 |
EuPathDBi | HostDB:ENSG00000154839.9 |
GeneCardsi | SKA1 |
HGNCi | HGNC:28109, SKA1 |
HPAi | ENSG00000154839, Tissue enhanced (lymphoid tissue, testis) |
MIMi | 616673, gene |
neXtProti | NX_Q96BD8 |
OpenTargetsi | ENSG00000154839 |
PharmGKBi | PA165429102 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4832, Eukaryota |
GeneTreei | ENSGT00390000011654 |
HOGENOMi | CLU_096842_0_0_1 |
InParanoidi | Q96BD8 |
OMAi | IGHHRVI |
PhylomeDBi | Q96BD8 |
TreeFami | TF324442 |
Enzyme and pathway databases
PathwayCommonsi | Q96BD8 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 220134, 448 hits in 854 CRISPR screens |
ChiTaRSi | SKA1, human |
GenomeRNAii | 220134 |
Pharosi | Q96BD8, Tbio |
PROi | PR:Q96BD8 |
RNActi | Q96BD8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000154839, Expressed in ventricular zone and 105 other tissues |
ExpressionAtlasi | Q96BD8, baseline and differential |
Genevisiblei | Q96BD8, HS |
Family and domain databases
Gene3Di | 1.10.10.1890, 1 hit |
InterProi | View protein in InterPro IPR009829, SKA1 IPR042031, SKA1-MBD |
PANTHERi | PTHR28573, PTHR28573, 1 hit |
Pfami | View protein in Pfam PF07160, SKA1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SKA1_HUMAN | |
Accessioni | Q96BD8Primary (citable) accession number: Q96BD8 Secondary accession number(s): B2R9Y6, B4E0P4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations