UniProtKB - Q96B86 (RGMA_HUMAN)
Protein
Repulsive guidance molecule A
Gene
RGMA
Organism
Homo sapiens (Human)
Status
Functioni
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.2 Publications
GO - Molecular functioni
- coreceptor activity Source: GO_Central
- transferrin receptor binding Source: BHF-UCL
GO - Biological processi
- BMP signaling pathway Source: GO_Central
- negative regulation of axon regeneration Source: InterPro
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Enzyme and pathway databases
PathwayCommonsi | Q96B86 |
Reactomei | R-HSA-373752, Netrin-1 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Repulsive guidance molecule AAlternative name(s): RGM domain family member A |
Gene namesi | Name:RGMA Synonyms:RGM |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30308, RGMA |
MIMi | 607362, gene |
neXtProti | NX_Q96B86 |
VEuPathDBi | HostDB:ENSG00000182175.13 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: LIFEdb
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- anchored component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 56963 |
PharmGKBi | PA128394693 |
Miscellaneous databases
Pharosi | Q96B86, Tbio |
Genetic variation databases
BioMutai | RGMA |
DMDMi | 296452979 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 47 | Sequence analysisAdd BLAST | 47 | |
PropeptideiPRO_0000030385 | 48 – 168 | Removed in mature formBy similarityAdd BLAST | 121 | |
ChainiPRO_0000030386 | 169 – 424 | Repulsive guidance molecule AAdd BLAST | 256 | |
PropeptideiPRO_0000030387 | 425 – 450 | Removed in mature formSequence analysisAdd BLAST | 26 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 114 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 145 ↔ 226 | By similarity | ||
Glycosylationi | 159 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 163 ↔ 315 | By similarity | ||
Glycosylationi | 389 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 424 | GPI-anchor amidated alanineSequence analysis | 1 |
Post-translational modificationi
Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 168 – 169 | Cleavage; by autolysisBy similarity | 2 |
Keywords - PTMi
Autocatalytic cleavage, Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
MassIVEi | Q96B86 |
PaxDbi | Q96B86 |
PeptideAtlasi | Q96B86 |
PRIDEi | Q96B86 |
ProteomicsDBi | 25133 33395 76054 [Q96B86-1] |
PTM databases
GlyGeni | Q96B86, 4 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q96B86 |
PhosphoSitePlusi | Q96B86 |
Expressioni
Gene expression databases
Bgeei | ENSG00000182175, Expressed in lower esophagus and 195 other tissues |
ExpressionAtlasi | Q96B86, baseline and differential |
Genevisiblei | Q96B86, HS |
Organism-specific databases
HPAi | ENSG00000182175, Tissue enhanced (brain, skeletal muscle) |
Interactioni
Subunit structurei
Interacts with NEO1, BMP2 and BMP4.
By similarityBinary interactionsi
Hide detailsQ96B86
With | #Exp. | IntAct |
---|---|---|
TERF2IP [Q9NYB0] | 2 | EBI-722102,EBI-750109 |
Isoform 1 [Q96B86-1]
With | #Exp. | IntAct |
---|---|---|
BMP2 [P12643] | 2 | EBI-16155394,EBI-1029262 |
Isoform 2 [Q96B86-3]
With | #Exp. | IntAct |
---|---|---|
TP53BP2 [Q05BL1] | 3 | EBI-14497860,EBI-11952721 |
GO - Molecular functioni
- transferrin receptor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 121284, 16 interactors |
DIPi | DIP-61606N |
IntActi | Q96B86, 16 interactors |
STRINGi | 9606.ENSP00000452126 |
Miscellaneous databases
RNActi | Q96B86, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96B86 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the repulsive guidance molecule (RGM) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QSTJ, Eukaryota |
HOGENOMi | CLU_032775_1_1_1 |
InParanoidi | Q96B86 |
OrthoDBi | 1300661at2759 |
PhylomeDBi | Q96B86 |
TreeFami | TF329836 |
Family and domain databases
Gene3Di | 3.40.1000.10, 1 hit |
InterProi | View protein in InterPro IPR016123, Mog1/PsbP_a/b/a-sand IPR040287, RGM IPR009496, RGM_C IPR010536, RGM_N IPR033607, RGMA |
PANTHERi | PTHR31428, PTHR31428, 1 hit PTHR31428:SF4, PTHR31428:SF4, 1 hit |
Pfami | View protein in Pfam PF06534, RGM_C, 1 hit PF06535, RGM_N, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96B86-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQPPRERLVV TGRAGWMGMG RGAGRSALGF WPTLAFLLCS FPAATSPCKI
60 70 80 90 100
LKCNSEFWSA TSGSHAPASD DTPEFCAALR SYALCTRRTA RTCRGDLAYH
110 120 130 140 150
SAVHGIEDLM SQHNCSKDGP TSQPRLRTLP PAGDSQERSD SPEICHYEKS
160 170 180 190 200
FHKHSATPNY THCGLFGDPH LRTFTDRFQT CKVQGAWPLI DNNYLNVQVT
210 220 230 240 250
NTPVLPGSAA TATSKLTIIF KNFQECVDQK VYQAEMDELP AAFVDGSKNG
260 270 280 290 300
GDKHGANSLK ITEKVSGQHV EIQAKYIGTT IVVRQVGRYL TFAVRMPEEV
310 320 330 340 350
VNAVEDWDSQ GLYLCLRGCP LNQQIDFQAF HTNAEGTGAR RLAAASPAPT
360 370 380 390 400
APETFPYETA VAKCKEKLPV EDLYYQACVF DLLTTGDVNF TLAAYYALED
410 420 430 440 450
VKMLHSNKDK LHLYDRTRDL PGRAAAGLPL APRPLLGALV PLLALLPVFC
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MTQ4 | A0A0A0MTQ4_HUMAN | Repulsive guidance molecule A | RGMA | 458 | Annotation score: | ||
F5H7G2 | F5H7G2_HUMAN | Repulsive guidance molecule A | RGMA | 341 | Annotation score: | ||
G3V4C2 | G3V4C2_HUMAN | Repulsive guidance molecule A | RGMA | 90 | Annotation score: | ||
G3V545 | G3V545_HUMAN | Repulsive guidance molecule A | RGMA | 46 | Annotation score: | ||
G3V3Z3 | G3V3Z3_HUMAN | Repulsive guidance molecule A | RGMA | 61 | Annotation score: | ||
A0A0G2JL92 | A0A0G2JL92_HUMAN | Repulsive guidance molecule A | RGMA | 10 | Annotation score: |
Sequence cautioni
The sequence AAH15886 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAB98207 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 199 | V → A in BAC11285 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 314 | L → P in BAH13014 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_062144 | 415 | D → E5 PublicationsCorresponds to variant dbSNP:rs4238485Ensembl. | 1 | |
Natural variantiVAR_060105 | 431 | A → V. Corresponds to variant dbSNP:rs4778078Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054070 | 1 – 16 | Missing in isoform 2. 3 PublicationsAdd BLAST | 16 | |
Alternative sequenceiVSP_054071 | 1 – 4 | MQPP → MGGLGPRRAGTS in isoform 3. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL390083 mRNA Translation: CAB98207.1 Different initiation. AL136826 mRNA Translation: CAB66760.1 AK074910 mRNA Translation: BAC11285.1 AK074966 mRNA Translation: BAC11321.1 AK074980 mRNA Translation: BAC11330.1 AK299363 mRNA Translation: BAH13014.1 AC087641 Genomic DNA No translation available. CH471101 Genomic DNA Translation: EAX02167.1 BC015886 mRNA Translation: AAH15886.2 Different initiation. BC140838 mRNA Translation: AAI40839.1 BC151132 mRNA Translation: AAI51133.1 |
CCDSi | CCDS45357.1 [Q96B86-1] CCDS53973.1 [Q96B86-3] CCDS53974.1 [Q96B86-4] |
RefSeqi | NP_001159755.1, NM_001166283.1 NP_001159758.1, NM_001166286.1 NP_001159759.1, NM_001166287.1 NP_001159760.1, NM_001166288.1 NP_001159761.1, NM_001166289.1 NP_064596.2, NM_020211.2 |
Genome annotation databases
Ensembli | ENST00000329082; ENSP00000330005; ENSG00000182175 ENST00000425933; ENSP00000404442; ENSG00000182175 ENST00000542321; ENSP00000440025; ENSG00000182175 ENST00000543599; ENSP00000442498; ENSG00000182175 |
GeneIDi | 56963 |
KEGGi | hsa:56963 |
UCSCi | uc002bsq.3, human [Q96B86-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL390083 mRNA Translation: CAB98207.1 Different initiation. AL136826 mRNA Translation: CAB66760.1 AK074910 mRNA Translation: BAC11285.1 AK074966 mRNA Translation: BAC11321.1 AK074980 mRNA Translation: BAC11330.1 AK299363 mRNA Translation: BAH13014.1 AC087641 Genomic DNA No translation available. CH471101 Genomic DNA Translation: EAX02167.1 BC015886 mRNA Translation: AAH15886.2 Different initiation. BC140838 mRNA Translation: AAI40839.1 BC151132 mRNA Translation: AAI51133.1 |
CCDSi | CCDS45357.1 [Q96B86-1] CCDS53973.1 [Q96B86-3] CCDS53974.1 [Q96B86-4] |
RefSeqi | NP_001159755.1, NM_001166283.1 NP_001159758.1, NM_001166286.1 NP_001159759.1, NM_001166287.1 NP_001159760.1, NM_001166288.1 NP_001159761.1, NM_001166289.1 NP_064596.2, NM_020211.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4UHY | X-ray | 3.20 | C | 46-139 | [»] | |
6Z3G | X-ray | 2.78 | B | 54-139 | [»] | |
SMRi | Q96B86 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121284, 16 interactors |
DIPi | DIP-61606N |
IntActi | Q96B86, 16 interactors |
STRINGi | 9606.ENSP00000452126 |
PTM databases
GlyGeni | Q96B86, 4 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q96B86 |
PhosphoSitePlusi | Q96B86 |
Genetic variation databases
BioMutai | RGMA |
DMDMi | 296452979 |
Proteomic databases
MassIVEi | Q96B86 |
PaxDbi | Q96B86 |
PeptideAtlasi | Q96B86 |
PRIDEi | Q96B86 |
ProteomicsDBi | 25133 33395 76054 [Q96B86-1] |
Protocols and materials databases
ABCDi | Q96B86, 1 sequenced antibody |
Antibodypediai | 43886, 249 antibodies |
DNASUi | 56963 |
Genome annotation databases
Ensembli | ENST00000329082; ENSP00000330005; ENSG00000182175 ENST00000425933; ENSP00000404442; ENSG00000182175 ENST00000542321; ENSP00000440025; ENSG00000182175 ENST00000543599; ENSP00000442498; ENSG00000182175 |
GeneIDi | 56963 |
KEGGi | hsa:56963 |
UCSCi | uc002bsq.3, human [Q96B86-1] |
Organism-specific databases
CTDi | 56963 |
DisGeNETi | 56963 |
GeneCardsi | RGMA |
HGNCi | HGNC:30308, RGMA |
HPAi | ENSG00000182175, Tissue enhanced (brain, skeletal muscle) |
MIMi | 607362, gene |
neXtProti | NX_Q96B86 |
PharmGKBi | PA128394693 |
VEuPathDBi | HostDB:ENSG00000182175.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSTJ, Eukaryota |
HOGENOMi | CLU_032775_1_1_1 |
InParanoidi | Q96B86 |
OrthoDBi | 1300661at2759 |
PhylomeDBi | Q96B86 |
TreeFami | TF329836 |
Enzyme and pathway databases
PathwayCommonsi | Q96B86 |
Reactomei | R-HSA-373752, Netrin-1 signaling |
Miscellaneous databases
BioGRID-ORCSi | 56963, 6 hits in 870 CRISPR screens |
ChiTaRSi | RGMA, human |
GeneWikii | RGMA |
GenomeRNAii | 56963 |
Pharosi | Q96B86, Tbio |
PROi | PR:Q96B86 |
RNActi | Q96B86, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182175, Expressed in lower esophagus and 195 other tissues |
ExpressionAtlasi | Q96B86, baseline and differential |
Genevisiblei | Q96B86, HS |
Family and domain databases
Gene3Di | 3.40.1000.10, 1 hit |
InterProi | View protein in InterPro IPR016123, Mog1/PsbP_a/b/a-sand IPR040287, RGM IPR009496, RGM_C IPR010536, RGM_N IPR033607, RGMA |
PANTHERi | PTHR31428, PTHR31428, 1 hit PTHR31428:SF4, PTHR31428:SF4, 1 hit |
Pfami | View protein in Pfam PF06534, RGM_C, 1 hit PF06535, RGM_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RGMA_HUMAN | |
Accessioni | Q96B86Primary (citable) accession number: Q96B86 Secondary accession number(s): B2RTW1 Q9NPM3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | May 18, 2010 | |
Last modified: | February 10, 2021 | |
This is version 141 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families