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Protein

Ribulose-phosphate 3-epimerase

Gene

RPE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.1 Publication

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.1 Publication

Cofactori

Fe2+1 Publication, Mn2+1 Publication, Zn2+1 Publication, Co2+1 PublicationNote: Binds 1 divalent metal cation per subunit. Active with Fe2+, and probably also with Mn2+, Zn2+ and Co2+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Substrate1
Metal bindingi35Divalent metal cation1
Active sitei37Proton acceptorCurated1
Metal bindingi37Divalent metal cation1
Metal bindingi70Divalent metal cation1
Binding sitei70Substrate1
Active sitei175Proton donorCurated1
Metal bindingi175Divalent metal cation1
Binding sitei177Substrate; via amide nitrogen1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • ribulose-phosphate 3-epimerase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processCarbohydrate metabolism
LigandCobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi5.1.3.1 2681
ReactomeiR-HSA-71336 Pentose phosphate pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase (EC:5.1.3.1)
Alternative name(s):
Ribulose-5-phosphate-3-epimerase
Gene namesi
Name:RPE
ORF Names:HUSSY-17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000197713.14
HGNCiHGNC:10293 RPE
MIMi180480 gene
neXtProtiNX_Q96AT9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10S → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi12L → A: Reduces enzyme activity by half. 1 Publication1
Mutagenesisi35H → A: Alters protein structure. Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi37D → A: Alters protein structure. Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi39M → A: Lowers enzyme activity by 10%. 1 Publication1
Mutagenesisi70H → A: Alters protein structure. 1 Publication1
Mutagenesisi72M → A: Reduces enzyme activity by half. 1 Publication1
Mutagenesisi141M → A: No effect on enzyme activity. 1 Publication1
Mutagenesisi175D → A: Alters protein structure. 1 Publication1

Organism-specific databases

DisGeNETi6120
OpenTargetsiENSG00000197713
PharmGKBiPA34654

Polymorphism and mutation databases

DMDMi34924986

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001715872 – 228Ribulose-phosphate 3-epimeraseAdd BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96AT9
MaxQBiQ96AT9
PaxDbiQ96AT9
PeptideAtlasiQ96AT9
PRIDEiQ96AT9
ProteomicsDBi75997
75998 [Q96AT9-2]
TopDownProteomicsiQ96AT9-1 [Q96AT9-1]
Q96AT9-2 [Q96AT9-2]

PTM databases

iPTMnetiQ96AT9
PhosphoSitePlusiQ96AT9

Expressioni

Gene expression databases

BgeeiENSG00000197713 Expressed in 215 organ(s), highest expression level in adrenal tissue
CleanExiHS_RPE
ExpressionAtlasiQ96AT9 baseline and differential
GenevisibleiQ96AT9 HS

Organism-specific databases

HPAiHPA036498
HPA036499

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-372480,EBI-372480

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112040, 51 interactors
IntActiQ96AT9, 4 interactors
STRINGi9606.ENSP00000352401

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96AT9
SMRiQ96AT9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96AT9

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni146 – 149Substrate binding4
Regioni197 – 198Substrate binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3111 Eukaryota
COG0036 LUCA
GeneTreeiENSGT00390000001447
HOGENOMiHOG000259349
HOVERGENiHBG044821
InParanoidiQ96AT9
KOiK01783
OMAiCHLMIED
OrthoDBiEOG091G0JMQ
PhylomeDBiQ96AT9
TreeFamiTF300157

Family and domain databases

CDDicd00429 RPE, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR026019 Ribul_P_3_epim
IPR000056 Ribul_P_3_epim-like
IPR011060 RibuloseP-bd_barrel
PANTHERiPTHR11749 PTHR11749, 1 hit
PfamiView protein in Pfam
PF00834 Ribul_P_3_epim, 1 hit
PIRSFiPIRSF001461 RPE, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR01163 rpe, 1 hit
PROSITEiView protein in PROSITE
PS01085 RIBUL_P_3_EPIMER_1, 1 hit
PS01086 RIBUL_P_3_EPIMER_2, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96AT9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASGCKIGPS ILNSDLANLG AECLRMLDSG ADYLHLDVMD GHFVPNITFG
60 70 80 90 100
HPVVESLRKQ LGQDPFFDMH MMVSKPEQWV KPMAVAGANQ YTFHLEATEN
110 120 130 140 150
PGALIKDIRE NGMKVGLAIK PGTSVEYLAP WANQIDMALV MTVEPGFGGQ
160 170 180 190 200
KFMEDMMPKV HWLRTQFPSL DIEVDGGVGP DTVHKCAEAG ANMIVSGSAI
210 220
MRSEDPRSVI NLLRNVCSEA AQKRSLDR
Length:228
Mass (Da):24,928
Last modified:December 1, 2001 - v1
Checksum:i447130018AC52331
GO
Isoform 2 (identifier: Q96AT9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-68: Missing.
     114-114: K → KSCSVTQAEVQWHSQGPLQ

Note: No experimental confirmation available.
Show »
Length:220
Mass (Da):23,924
Checksum:i626FAFD9426AE406
GO
Isoform 3 (identifier: Q96AT9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.
     114-114: K → KSCSVTQAEVQWHSQGPLQ

Note: Gene prediction based on EST data.
Show »
Length:178
Mass (Da):19,537
Checksum:iC2D4353C7FFAEC41
GO
Isoform 4 (identifier: Q96AT9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-188: Missing.

Note: No experimental confirmation available.
Show »
Length:199
Mass (Da):21,739
Checksum:i88D6C3598F28A1E1
GO
Isoform 5 (identifier: Q96AT9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Note: Gene prediction based on EST data.
Show »
Length:160
Mass (Da):17,570
Checksum:i9B6890E5E78B7D35
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9IZU8C9IZU8_HUMAN
Ribulose-phosphate 3-epimerase
RPE
225Annotation score:
C9J9T0C9J9T0_HUMAN
Ribulose-5-phosphate-3-epimerase, i...
RPE hCG_2012337
191Annotation score:
C9IYE8C9IYE8_HUMAN
Ribulose-phosphate 3-epimerase
RPE
141Annotation score:
C9JCL8C9JCL8_HUMAN
Ribulose-phosphate 3-epimerase
RPE
107Annotation score:
C9J6A7C9J6A7_HUMAN
Ribulose-phosphate 3-epimerase
RPE
159Annotation score:
C9J8S0C9J8S0_HUMAN
Ribulose-phosphate 3-epimerase
RPE
157Annotation score:
E7ESZ6E7ESZ6_HUMAN
Ribulose-phosphate 3-epimerase
RPE
147Annotation score:
E9PBG9E9PBG9_HUMAN
Ribulose-phosphate 3-epimerase
RPE
94Annotation score:
F8WBT4F8WBT4_HUMAN
Ribulose-phosphate 3-epimerase
RPE
60Annotation score:

Sequence cautioni

The sequence BAB71076 differs from that shown. Reason: Frameshift at position 69.Curated
The sequence BAC04212 differs from that shown. Reason: Frameshift at position 42.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180P → L in BAC04212 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0471171 – 68Missing in isoform 3 and isoform 5. CuratedAdd BLAST68
Alternative sequenceiVSP_00831743 – 68Missing in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_008318114K → KSCSVTQAEVQWHSQGPLQ in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_055265160 – 188Missing in isoform 4. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056028 mRNA Translation: BAB71076.1 Frameshift.
AK093658 mRNA Translation: BAC04212.1 Frameshift.
AK291035 mRNA Translation: BAF83724.1
AK303184 mRNA Translation: BAG64278.1
AC007038 Genomic DNA Translation: AAX93087.1
CH471063 Genomic DNA Translation: EAW70473.1
CH471063 Genomic DNA Translation: EAW70474.1
BC005148 mRNA Translation: AAH05148.2
BC016764 mRNA Translation: AAH16764.1
BC072401 mRNA Translation: AAH72401.1
AJ224326 mRNA Translation: CAA11895.1
CCDSiCCDS2388.1 [Q96AT9-1]
CCDS42810.1 [Q96AT9-3]
CCDS63107.1 [Q96AT9-4]
CCDS63108.1 [Q96AT9-5]
RefSeqiNP_001265211.1, NM_001278282.1 [Q96AT9-3]
NP_001265212.1, NM_001278283.1 [Q96AT9-3]
NP_001265214.1, NM_001278285.1 [Q96AT9-4]
NP_001265215.1, NM_001278286.1 [Q96AT9-5]
NP_001265217.1, NM_001278288.1 [Q96AT9-5]
NP_001265218.1, NM_001278289.1
NP_001305855.1, NM_001318926.1
NP_001305856.1, NM_001318927.1
NP_001305857.1, NM_001318928.1
NP_001305858.1, NM_001318929.1
NP_001305859.1, NM_001318930.1 [Q96AT9-5]
NP_001305860.1, NM_001318931.1 [Q96AT9-5]
NP_008847.1, NM_006916.2 [Q96AT9-3]
NP_954699.1, NM_199229.2 [Q96AT9-1]
XP_006712740.1, XM_006712677.3 [Q96AT9-5]
UniGeneiHs.282260
Hs.591638
Hs.734255

Genome annotation databases

EnsembliENST00000354506; ENSP00000346501; ENSG00000197713 [Q96AT9-4]
ENST00000359429; ENSP00000352401; ENSG00000197713 [Q96AT9-1]
ENST00000411934; ENSP00000389411; ENSG00000197713 [Q96AT9-5]
ENST00000429921; ENSP00000401838; ENSG00000197713 [Q96AT9-3]
ENST00000436630; ENSP00000403808; ENSG00000197713 [Q96AT9-3]
ENST00000438204; ENSP00000402061; ENSG00000197713 [Q96AT9-5]
ENST00000454822; ENSP00000394455; ENSG00000197713 [Q96AT9-3]
GeneIDi6120
KEGGihsa:6120
UCSCiuc002vdn.5 human [Q96AT9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056028 mRNA Translation: BAB71076.1 Frameshift.
AK093658 mRNA Translation: BAC04212.1 Frameshift.
AK291035 mRNA Translation: BAF83724.1
AK303184 mRNA Translation: BAG64278.1
AC007038 Genomic DNA Translation: AAX93087.1
CH471063 Genomic DNA Translation: EAW70473.1
CH471063 Genomic DNA Translation: EAW70474.1
BC005148 mRNA Translation: AAH05148.2
BC016764 mRNA Translation: AAH16764.1
BC072401 mRNA Translation: AAH72401.1
AJ224326 mRNA Translation: CAA11895.1
CCDSiCCDS2388.1 [Q96AT9-1]
CCDS42810.1 [Q96AT9-3]
CCDS63107.1 [Q96AT9-4]
CCDS63108.1 [Q96AT9-5]
RefSeqiNP_001265211.1, NM_001278282.1 [Q96AT9-3]
NP_001265212.1, NM_001278283.1 [Q96AT9-3]
NP_001265214.1, NM_001278285.1 [Q96AT9-4]
NP_001265215.1, NM_001278286.1 [Q96AT9-5]
NP_001265217.1, NM_001278288.1 [Q96AT9-5]
NP_001265218.1, NM_001278289.1
NP_001305855.1, NM_001318926.1
NP_001305856.1, NM_001318927.1
NP_001305857.1, NM_001318928.1
NP_001305858.1, NM_001318929.1
NP_001305859.1, NM_001318930.1 [Q96AT9-5]
NP_001305860.1, NM_001318931.1 [Q96AT9-5]
NP_008847.1, NM_006916.2 [Q96AT9-3]
NP_954699.1, NM_199229.2 [Q96AT9-1]
XP_006712740.1, XM_006712677.3 [Q96AT9-5]
UniGeneiHs.282260
Hs.591638
Hs.734255

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OVPX-ray1.70A/B1-228[»]
3OVQX-ray2.00A/B1-228[»]
3OVRX-ray1.95A/B1-228[»]
3QC3X-ray2.20A/B1-224[»]
ProteinModelPortaliQ96AT9
SMRiQ96AT9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112040, 51 interactors
IntActiQ96AT9, 4 interactors
STRINGi9606.ENSP00000352401

PTM databases

iPTMnetiQ96AT9
PhosphoSitePlusiQ96AT9

Polymorphism and mutation databases

DMDMi34924986

Proteomic databases

EPDiQ96AT9
MaxQBiQ96AT9
PaxDbiQ96AT9
PeptideAtlasiQ96AT9
PRIDEiQ96AT9
ProteomicsDBi75997
75998 [Q96AT9-2]
TopDownProteomicsiQ96AT9-1 [Q96AT9-1]
Q96AT9-2 [Q96AT9-2]

Protocols and materials databases

DNASUi6120
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354506; ENSP00000346501; ENSG00000197713 [Q96AT9-4]
ENST00000359429; ENSP00000352401; ENSG00000197713 [Q96AT9-1]
ENST00000411934; ENSP00000389411; ENSG00000197713 [Q96AT9-5]
ENST00000429921; ENSP00000401838; ENSG00000197713 [Q96AT9-3]
ENST00000436630; ENSP00000403808; ENSG00000197713 [Q96AT9-3]
ENST00000438204; ENSP00000402061; ENSG00000197713 [Q96AT9-5]
ENST00000454822; ENSP00000394455; ENSG00000197713 [Q96AT9-3]
GeneIDi6120
KEGGihsa:6120
UCSCiuc002vdn.5 human [Q96AT9-1]

Organism-specific databases

CTDi6120
DisGeNETi6120
EuPathDBiHostDB:ENSG00000197713.14
GeneCardsiRPE
H-InvDBiHIX0190555
HGNCiHGNC:10293 RPE
HPAiHPA036498
HPA036499
MIMi180480 gene
neXtProtiNX_Q96AT9
OpenTargetsiENSG00000197713
PharmGKBiPA34654
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3111 Eukaryota
COG0036 LUCA
GeneTreeiENSGT00390000001447
HOGENOMiHOG000259349
HOVERGENiHBG044821
InParanoidiQ96AT9
KOiK01783
OMAiCHLMIED
OrthoDBiEOG091G0JMQ
PhylomeDBiQ96AT9
TreeFamiTF300157

Enzyme and pathway databases

BRENDAi5.1.3.1 2681
ReactomeiR-HSA-71336 Pentose phosphate pathway

Miscellaneous databases

EvolutionaryTraceiQ96AT9
GeneWikiiRPE_(gene)
GenomeRNAii6120
PROiPR:Q96AT9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197713 Expressed in 215 organ(s), highest expression level in adrenal tissue
CleanExiHS_RPE
ExpressionAtlasiQ96AT9 baseline and differential
GenevisibleiQ96AT9 HS

Family and domain databases

CDDicd00429 RPE, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR026019 Ribul_P_3_epim
IPR000056 Ribul_P_3_epim-like
IPR011060 RibuloseP-bd_barrel
PANTHERiPTHR11749 PTHR11749, 1 hit
PfamiView protein in Pfam
PF00834 Ribul_P_3_epim, 1 hit
PIRSFiPIRSF001461 RPE, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR01163 rpe, 1 hit
PROSITEiView protein in PROSITE
PS01085 RIBUL_P_3_EPIMER_1, 1 hit
PS01086 RIBUL_P_3_EPIMER_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPE_HUMAN
AccessioniPrimary (citable) accession number: Q96AT9
Secondary accession number(s): A8K4S0
, B4E016, C9JPQ7, O43767, Q53TV9, Q8N215, Q96N34, Q9BSB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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