UniProtKB - Q96AT9 (RPE_HUMAN)
Protein
Ribulose-phosphate 3-epimerase
Gene
RPE
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.1 Publication
Catalytic activityi
- EC:5.1.3.11 Publication
Cofactori
Fe2+1 Publication, Mn2+1 Publication, Zn2+1 Publication, Co2+1 PublicationNote: Binds 1 divalent metal cation per subunit. Active with Fe2+, and probably also with Mn2+, Zn2+ and Co2+.1 Publication
: Carbohydrate degradation Pathwayi
This protein is involved in Carbohydrate degradation.1 PublicationView all proteins of this organism that are known to be involved in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 10 | SubstrateCombined sources1 Publication | 1 | |
Metal bindingi | 35 | Divalent metal cation; via tele nitrogenCombined sources2 Publications | 1 | |
Active sitei | 37 | Proton acceptorCombined sources1 Publication | 1 | |
Metal bindingi | 37 | Divalent metal cationCombined sources2 Publications | 1 | |
Metal bindingi | 70 | Divalent metal cation; via pros nitrogenCombined sources2 Publications | 1 | |
Binding sitei | 70 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 175 | Proton donorCombined sources1 Publication | 1 | |
Metal bindingi | 175 | Divalent metal cationCombined sources2 Publications | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- ribulose-phosphate 3-epimerase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB
- cellular carbohydrate metabolic process Source: GO_Central
- pentose catabolic process Source: GO_Central
- pentose-phosphate shunt Source: UniProtKB
- pentose-phosphate shunt, non-oxidative branch Source: GO_Central
Keywordsi
Molecular function | Isomerase |
Biological process | Carbohydrate metabolism |
Ligand | Cobalt, Iron, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 5.1.3.1, 2681 |
PathwayCommonsi | Q96AT9 |
Reactomei | R-HSA-71336, Pentose phosphate pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Ribulose-phosphate 3-epimerase (EC:5.1.3.11 Publication)Alternative name(s): Ribulose-5-phosphate-3-epimerase |
Gene namesi | Name:RPE ORF Names:HUSSY-17 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000197713.14 |
HGNCi | HGNC:10293, RPE |
MIMi | 180480, gene |
neXtProti | NX_Q96AT9 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | S → A: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 12 | L → A: Reduces enzyme activity by half. 1 Publication | 1 | |
Mutagenesisi | 35 | H → A: Alters protein structure. Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 37 | D → A: Alters protein structure. Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 39 | M → A: Lowers enzyme activity by 10%. 1 Publication | 1 | |
Mutagenesisi | 70 | H → A: Alters protein structure. 1 Publication | 1 | |
Mutagenesisi | 72 | M → A: Reduces enzyme activity by half. 1 Publication | 1 | |
Mutagenesisi | 141 | M → A: No effect on enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 175 | D → A: Alters protein structure. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6120 |
OpenTargetsi | ENSG00000197713 |
PharmGKBi | PA34654 |
Miscellaneous databases
Pharosi | Q96AT9, Tbio |
Chemistry databases
DrugBanki | DB00153, Ergocalciferol |
Polymorphism and mutation databases
BioMutai | RPE |
DMDMi | 34924986 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000171587 | 2 – 228 | Ribulose-phosphate 3-epimeraseAdd BLAST | 227 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q96AT9 |
jPOSTi | Q96AT9 |
MassIVEi | Q96AT9 |
MaxQBi | Q96AT9 |
PaxDbi | Q96AT9 |
PeptideAtlasi | Q96AT9 |
PRIDEi | Q96AT9 |
ProteomicsDBi | 11146 5637 62553 75997 [Q96AT9-1] 75998 [Q96AT9-2] |
TopDownProteomicsi | Q96AT9-1 [Q96AT9-1] Q96AT9-2 [Q96AT9-2] |
PTM databases
iPTMneti | Q96AT9 |
PhosphoSitePlusi | Q96AT9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000197713, Expressed in adrenal tissue and 228 other tissues |
ExpressionAtlasi | Q96AT9, baseline and differential |
Genevisiblei | Q96AT9, HS |
Organism-specific databases
HPAi | ENSG00000197713, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Hide detailsQ96AT9
With | #Exp. | IntAct |
---|---|---|
PLEKHF2 [Q9H8W4] | 3 | EBI-372480,EBI-742388 |
itself | 6 | EBI-372480,EBI-372480 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 112040, 53 interactors |
IntActi | Q96AT9, 6 interactors |
STRINGi | 9606.ENSP00000352401 |
Miscellaneous databases
RNActi | Q96AT9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96AT9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96AT9 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 146 – 149 | Substrate bindingCombined sources1 Publication | 4 | |
Regioni | 175 – 177 | Substrate bindingCombined sources1 Publication | 3 | |
Regioni | 197 – 198 | Substrate bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the ribulose-phosphate 3-epimerase family.Curated
Phylogenomic databases
eggNOGi | KOG3111, Eukaryota |
GeneTreei | ENSGT00390000001447 |
InParanoidi | Q96AT9 |
OMAi | WLQVDGG |
OrthoDBi | 1554029at2759 |
PhylomeDBi | Q96AT9 |
TreeFami | TF300157 |
Family and domain databases
CDDi | cd00429, RPE, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_02227, RPE, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR026019, Ribul_P_3_epim IPR000056, Ribul_P_3_epim-like IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR11749, PTHR11749, 1 hit |
Pfami | View protein in Pfam PF00834, Ribul_P_3_epim, 1 hit |
PIRSFi | PIRSF001461, RPE, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR01163, rpe, 1 hit |
PROSITEi | View protein in PROSITE PS01085, RIBUL_P_3_EPIMER_1, 1 hit PS01086, RIBUL_P_3_EPIMER_2, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96AT9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASGCKIGPS ILNSDLANLG AECLRMLDSG ADYLHLDVMD GHFVPNITFG
60 70 80 90 100
HPVVESLRKQ LGQDPFFDMH MMVSKPEQWV KPMAVAGANQ YTFHLEATEN
110 120 130 140 150
PGALIKDIRE NGMKVGLAIK PGTSVEYLAP WANQIDMALV MTVEPGFGGQ
160 170 180 190 200
KFMEDMMPKV HWLRTQFPSL DIEVDGGVGP DTVHKCAEAG ANMIVSGSAI
210 220
MRSEDPRSVI NLLRNVCSEA AQKRSLDR
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9IZU8 | C9IZU8_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 225 | Annotation score: | ||
C9J9T0 | C9J9T0_HUMAN | Ribulose-phosphate 3-epimerase | RPE hCG_2012337 | 191 | Annotation score: | ||
C9JCL8 | C9JCL8_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 107 | Annotation score: | ||
C9IYE8 | C9IYE8_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 141 | Annotation score: | ||
C9J6A7 | C9J6A7_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 159 | Annotation score: | ||
C9J8S0 | C9J8S0_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 157 | Annotation score: | ||
E7ESZ6 | E7ESZ6_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 147 | Annotation score: | ||
E9PBG9 | E9PBG9_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 94 | Annotation score: | ||
F8WBT4 | F8WBT4_HUMAN | Ribulose-phosphate 3-epimerase | RPE | 60 | Annotation score: |
Sequence cautioni
The sequence BAB71076 differs from that shown. Reason: Frameshift.Curated
The sequence BAC04212 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 180 | P → L in BAC04212 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047117 | 1 – 68 | Missing in isoform 3 and isoform 5. CuratedAdd BLAST | 68 | |
Alternative sequenceiVSP_008317 | 43 – 68 | Missing in isoform 2. 1 PublicationAdd BLAST | 26 | |
Alternative sequenceiVSP_008318 | 114 | K → KSCSVTQAEVQWHSQGPLQ in isoform 2 and isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_055265 | 160 – 188 | Missing in isoform 4. 1 PublicationAdd BLAST | 29 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000354506; ENSP00000346501; ENSG00000197713 [Q96AT9-4] ENST00000359429; ENSP00000352401; ENSG00000197713 [Q96AT9-1] ENST00000411934; ENSP00000389411; ENSG00000197713 [Q96AT9-5] ENST00000429921; ENSP00000401838; ENSG00000197713 [Q96AT9-3] ENST00000436630; ENSP00000403808; ENSG00000197713 [Q96AT9-3] ENST00000438204; ENSP00000402061; ENSG00000197713 [Q96AT9-5] ENST00000454822; ENSP00000394455; ENSG00000197713 [Q96AT9-3] |
GeneIDi | 6120 |
KEGGi | hsa:6120 |
UCSCi | uc002vdn.5, human [Q96AT9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3OVP | X-ray | 1.70 | A/B | 1-228 | [»] | |
3OVQ | X-ray | 2.00 | A/B | 1-228 | [»] | |
3OVR | X-ray | 1.95 | A/B | 1-228 | [»] | |
3QC3 | X-ray | 2.20 | A/B | 1-224 | [»] | |
SMRi | Q96AT9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112040, 53 interactors |
IntActi | Q96AT9, 6 interactors |
STRINGi | 9606.ENSP00000352401 |
Chemistry databases
DrugBanki | DB00153, Ergocalciferol |
PTM databases
iPTMneti | Q96AT9 |
PhosphoSitePlusi | Q96AT9 |
Polymorphism and mutation databases
BioMutai | RPE |
DMDMi | 34924986 |
Proteomic databases
EPDi | Q96AT9 |
jPOSTi | Q96AT9 |
MassIVEi | Q96AT9 |
MaxQBi | Q96AT9 |
PaxDbi | Q96AT9 |
PeptideAtlasi | Q96AT9 |
PRIDEi | Q96AT9 |
ProteomicsDBi | 11146 5637 62553 75997 [Q96AT9-1] 75998 [Q96AT9-2] |
TopDownProteomicsi | Q96AT9-1 [Q96AT9-1] Q96AT9-2 [Q96AT9-2] |
Protocols and materials databases
Antibodypediai | 34203, 121 antibodies |
DNASUi | 6120 |
Genome annotation databases
Organism-specific databases
CTDi | 6120 |
DisGeNETi | 6120 |
EuPathDBi | HostDB:ENSG00000197713.14 |
GeneCardsi | RPE |
HGNCi | HGNC:10293, RPE |
HPAi | ENSG00000197713, Low tissue specificity |
MIMi | 180480, gene |
neXtProti | NX_Q96AT9 |
OpenTargetsi | ENSG00000197713 |
PharmGKBi | PA34654 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3111, Eukaryota |
GeneTreei | ENSGT00390000001447 |
InParanoidi | Q96AT9 |
OMAi | WLQVDGG |
OrthoDBi | 1554029at2759 |
PhylomeDBi | Q96AT9 |
TreeFami | TF300157 |
Enzyme and pathway databases
BRENDAi | 5.1.3.1, 2681 |
PathwayCommonsi | Q96AT9 |
Reactomei | R-HSA-71336, Pentose phosphate pathway |
Miscellaneous databases
BioGRID-ORCSi | 6120, 578 hits in 849 CRISPR screens |
ChiTaRSi | RPE, human |
EvolutionaryTracei | Q96AT9 |
GeneWikii | RPE_(gene) |
GenomeRNAii | 6120 |
Pharosi | Q96AT9, Tbio |
PROi | PR:Q96AT9 |
RNActi | Q96AT9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197713, Expressed in adrenal tissue and 228 other tissues |
ExpressionAtlasi | Q96AT9, baseline and differential |
Genevisiblei | Q96AT9, HS |
Family and domain databases
CDDi | cd00429, RPE, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_02227, RPE, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR026019, Ribul_P_3_epim IPR000056, Ribul_P_3_epim-like IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR11749, PTHR11749, 1 hit |
Pfami | View protein in Pfam PF00834, Ribul_P_3_epim, 1 hit |
PIRSFi | PIRSF001461, RPE, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR01163, rpe, 1 hit |
PROSITEi | View protein in PROSITE PS01085, RIBUL_P_3_EPIMER_1, 1 hit PS01086, RIBUL_P_3_EPIMER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPE_HUMAN | |
Accessioni | Q96AT9Primary (citable) accession number: Q96AT9 Secondary accession number(s): A8K4S0 Q9BSB5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM