UniProtKB - Q96AB6 (NTAN1_HUMAN)
Protein N-terminal asparagine amidohydrolase
NTAN1
Functioni
N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.
1 PublicationCatalytic activityi
- EC:3.5.1.1211 Publication
Activity regulationi
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 75 | Essential for catalytic activity | 1 |
GO - Molecular functioni
- protein-N-terminal asparagine amidohydrolase activity Source: UniProtKB
GO - Biological processi
- adult locomotory behavior Source: Ensembl
- memory Source: Ensembl
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BRENDAi | 3.5.1.121, 2681 |
PathwayCommonsi | Q96AB6 |
SignaLinki | Q96AB6 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein N-terminal asparagine amidohydrolase (EC:3.5.1.1211 Publication)Alternative name(s): Protein NH2-terminal asparagine amidohydrolase Short name: PNAA Protein NH2-terminal asparagine deamidase Short name: PNAD Short name: Protein N-terminal Asn amidase Short name: Protein N-terminal asparagine amidase Short name: Protein NTN-amidase |
Gene namesi | Name:NTAN1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29909, NTAN1 |
MIMi | 615367, gene |
neXtProti | NX_Q96AB6 |
VEuPathDBi | HostDB:ENSG00000157045 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | P → G: 3-fold reduction in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 75 | C → A, S or T: Abolishes catalytic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 123803 |
OpenTargetsi | ENSG00000157045 |
PharmGKBi | PA134863573 |
Miscellaneous databases
Pharosi | Q96AB6, Tbio |
Genetic variation databases
BioMutai | NTAN1 |
DMDMi | 37082118 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057971 | 1 – 310 | Protein N-terminal asparagine amidohydrolaseAdd BLAST | 310 |
Proteomic databases
EPDi | Q96AB6 |
jPOSTi | Q96AB6 |
MassIVEi | Q96AB6 |
MaxQBi | Q96AB6 |
PaxDbi | Q96AB6 |
PeptideAtlasi | Q96AB6 |
PRIDEi | Q96AB6 |
ProteomicsDBi | 75948 |
PTM databases
iPTMneti | Q96AB6 |
PhosphoSitePlusi | Q96AB6 |
Expressioni
Gene expression databases
Bgeei | ENSG00000157045, Expressed in testis and 239 other tissues |
ExpressionAtlasi | Q96AB6, baseline and differential |
Genevisiblei | Q96AB6, HS |
Organism-specific databases
HPAi | ENSG00000157045, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
By similarityBinary interactionsi
Q96AB6
With | #Exp. | IntAct |
---|---|---|
MEOX2 [Q6FHY5] | 3 | EBI-12401218,EBI-16439278 |
Protein-protein interaction databases
BioGRIDi | 125835, 10 interactors |
IntActi | Q96AB6, 2 interactors |
STRINGi | 9606.ENSP00000287706 |
Miscellaneous databases
RNActi | Q96AB6, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q96AB6 |
SMRi | Q96AB6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502QSQW, Eukaryota |
GeneTreei | ENSGT00390000016730 |
HOGENOMi | CLU_077981_1_0_1 |
InParanoidi | Q96AB6 |
OMAi | YVQQREM |
OrthoDBi | 804210at2759 |
PhylomeDBi | Q96AB6 |
TreeFami | TF325597 |
Family and domain databases
InterProi | View protein in InterPro IPR026750, NTAN1 |
PANTHERi | PTHR12498, PTHR12498, 1 hit |
Pfami | View protein in Pfam PF14736, N_Asn_amidohyd, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPLLVEGRRV RLPQSAGDLV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ
60 70 80 90 100
QRELAVTSPK DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGTDTKA
110 120 130 140 150
EVPLIMNSIK SFSDHAQCGR LEVHLVGGFS DDRQLSQKLT HQLLSEFDRQ
160 170 180 190 200
EDDIHLVTLC VTELNDREEN ENHFPVIYGI AVNIKTAEIY RASFQDRGPE
210 220 230 240 250
EQLRAARTLA GGPMISIYDA ETEQLRIGPY SWTPFPHVDF WLHQDDKQIL
260 270 280 290 300
ENLSTSPLAE PPHFVEHIRS TLMFLKKHPS PAHTLFSGNK ALLYKKNEDG
310
LWEKISSPGS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087X0T5 | A0A087X0T5_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 205 | Annotation score: | ||
H3BPN7 | H3BPN7_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 146 | Annotation score: | ||
H3BNJ5 | H3BNJ5_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 223 | Annotation score: | ||
H3BMX8 | H3BMX8_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 18 | Annotation score: | ||
H3BU50 | H3BU50_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 45 | Annotation score: | ||
H3BR97 | H3BR97_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 27 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | E → D in AAP97215 (Ref. 1) Curated | 1 | |
Sequence conflicti | 14 | Q → R in AAP97215 (Ref. 1) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051244 | 283 | H → N. Corresponds to variant dbSNP:rs1136001Ensembl. | 1 | |
Natural variantiVAR_051245 | 287 | S → P. Corresponds to variant dbSNP:rs1135999Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092440 mRNA Translation: AAP97215.1 BC017336 mRNA Translation: AAH17336.1 |
CCDSi | CCDS10558.1 |
RefSeqi | NP_001257695.1, NM_001270766.1 NP_001257696.1, NM_001270767.1 NP_775745.1, NM_173474.3 |
Genome annotation databases
Ensembli | ENST00000287706.8; ENSP00000287706.3; ENSG00000157045.9 ENST00000620176.4; ENSP00000484380.1; ENSG00000275779.4 |
GeneIDi | 123803 |
KEGGi | hsa:123803 |
MANE-Selecti | ENST00000287706.8; ENSP00000287706.3; NM_173474.4; NP_775745.1 |
UCSCi | uc002ddd.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092440 mRNA Translation: AAP97215.1 BC017336 mRNA Translation: AAH17336.1 |
CCDSi | CCDS10558.1 |
RefSeqi | NP_001257695.1, NM_001270766.1 NP_001257696.1, NM_001270767.1 NP_775745.1, NM_173474.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6A0E | X-ray | 1.95 | A/B | 1-310 | [»] | |
6A0F | X-ray | 2.38 | A/B | 1-310 | [»] | |
6A0H | X-ray | 3.19 | A/B | 1-310 | [»] | |
6A0I | X-ray | 2.00 | A/B | 1-310 | [»] | |
AlphaFoldDBi | Q96AB6 | |||||
SMRi | Q96AB6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125835, 10 interactors |
IntActi | Q96AB6, 2 interactors |
STRINGi | 9606.ENSP00000287706 |
PTM databases
iPTMneti | Q96AB6 |
PhosphoSitePlusi | Q96AB6 |
Genetic variation databases
BioMutai | NTAN1 |
DMDMi | 37082118 |
Proteomic databases
EPDi | Q96AB6 |
jPOSTi | Q96AB6 |
MassIVEi | Q96AB6 |
MaxQBi | Q96AB6 |
PaxDbi | Q96AB6 |
PeptideAtlasi | Q96AB6 |
PRIDEi | Q96AB6 |
ProteomicsDBi | 75948 |
Protocols and materials databases
Antibodypediai | 24973, 97 antibodies from 19 providers |
DNASUi | 123803 |
Genome annotation databases
Ensembli | ENST00000287706.8; ENSP00000287706.3; ENSG00000157045.9 ENST00000620176.4; ENSP00000484380.1; ENSG00000275779.4 |
GeneIDi | 123803 |
KEGGi | hsa:123803 |
MANE-Selecti | ENST00000287706.8; ENSP00000287706.3; NM_173474.4; NP_775745.1 |
UCSCi | uc002ddd.5, human |
Organism-specific databases
CTDi | 123803 |
DisGeNETi | 123803 |
GeneCardsi | NTAN1 |
HGNCi | HGNC:29909, NTAN1 |
HPAi | ENSG00000157045, Low tissue specificity |
MIMi | 615367, gene |
neXtProti | NX_Q96AB6 |
OpenTargetsi | ENSG00000157045 |
PharmGKBi | PA134863573 |
VEuPathDBi | HostDB:ENSG00000157045 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSQW, Eukaryota |
GeneTreei | ENSGT00390000016730 |
HOGENOMi | CLU_077981_1_0_1 |
InParanoidi | Q96AB6 |
OMAi | YVQQREM |
OrthoDBi | 804210at2759 |
PhylomeDBi | Q96AB6 |
TreeFami | TF325597 |
Enzyme and pathway databases
BRENDAi | 3.5.1.121, 2681 |
PathwayCommonsi | Q96AB6 |
SignaLinki | Q96AB6 |
Miscellaneous databases
BioGRID-ORCSi | 123803, 10 hits in 1078 CRISPR screens |
ChiTaRSi | NTAN1, human |
GenomeRNAii | 123803 |
Pharosi | Q96AB6, Tbio |
PROi | PR:Q96AB6 |
RNActi | Q96AB6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000157045, Expressed in testis and 239 other tissues |
ExpressionAtlasi | Q96AB6, baseline and differential |
Genevisiblei | Q96AB6, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026750, NTAN1 |
PANTHERi | PTHR12498, PTHR12498, 1 hit |
Pfami | View protein in Pfam PF14736, N_Asn_amidohyd, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NTAN1_HUMAN | |
Accessioni | Q96AB6Primary (citable) accession number: Q96AB6 Secondary accession number(s): Q7Z4Z0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references