UniProtKB - Q96AB6 (NTAN1_HUMAN)
Protein
Protein N-terminal asparagine amidohydrolase
Gene
NTAN1
Organism
Homo sapiens (Human)
Status
Functioni
N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.1 Publication
Catalytic activityi
- EC:3.5.1.1211 Publication
Activity regulationi
Inhibited by micromolar concentrations of copper and zinc ions.1 Publication
pH dependencei
Optimum pH is 6.0-7.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 75 | Essential for catalytic activity | 1 |
GO - Molecular functioni
- protein-N-terminal asparagine amidohydrolase activity Source: UniProtKB
GO - Biological processi
- adult locomotory behavior Source: Ensembl
- memory Source: Ensembl
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000157045-MONOMER |
PathwayCommonsi | Q96AB6 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein N-terminal asparagine amidohydrolase (EC:3.5.1.1211 Publication)Alternative name(s): Protein NH2-terminal asparagine amidohydrolase Short name: PNAA Protein NH2-terminal asparagine deamidase Short name: PNAD Short name: Protein N-terminal Asn amidase Short name: Protein N-terminal asparagine amidase Short name: Protein NTN-amidase |
Gene namesi | Name:NTAN1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000157045.8 |
HGNCi | HGNC:29909, NTAN1 |
MIMi | 615367, gene |
neXtProti | NX_Q96AB6 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | P → G: 3-fold reduction in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 75 | C → A, S or T: Abolishes catalytic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 123803 |
OpenTargetsi | ENSG00000157045 |
PharmGKBi | PA134863573 |
Miscellaneous databases
Pharosi | Q96AB6, Tbio |
Polymorphism and mutation databases
BioMutai | NTAN1 |
DMDMi | 37082118 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057971 | 1 – 310 | Protein N-terminal asparagine amidohydrolaseAdd BLAST | 310 |
Proteomic databases
EPDi | Q96AB6 |
jPOSTi | Q96AB6 |
MassIVEi | Q96AB6 |
MaxQBi | Q96AB6 |
PaxDbi | Q96AB6 |
PeptideAtlasi | Q96AB6 |
PRIDEi | Q96AB6 |
ProteomicsDBi | 75948 |
PTM databases
iPTMneti | Q96AB6 |
PhosphoSitePlusi | Q96AB6 |
Expressioni
Gene expression databases
Bgeei | ENSG00000157045, Expressed in testis and 239 other tissues |
ExpressionAtlasi | Q96AB6, baseline and differential |
Genevisiblei | Q96AB6, HS |
Organism-specific databases
HPAi | ENSG00000157045, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
By similarityBinary interactionsi
Q96AB6
With | #Exp. | IntAct |
---|---|---|
MEOX2 [Q6FHY5] | 3 | EBI-12401218,EBI-16439278 |
Protein-protein interaction databases
BioGRIDi | 125835, 3 interactors |
IntActi | Q96AB6, 2 interactors |
STRINGi | 9606.ENSP00000287706 |
Miscellaneous databases
RNActi | Q96AB6, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96AB6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502QSQW, Eukaryota |
GeneTreei | ENSGT00390000016730 |
HOGENOMi | CLU_077981_1_0_1 |
InParanoidi | Q96AB6 |
OMAi | VCLAHCD |
OrthoDBi | 804210at2759 |
PhylomeDBi | Q96AB6 |
TreeFami | TF325597 |
Family and domain databases
InterProi | View protein in InterPro IPR026750, NTAN1 |
PANTHERi | PTHR12498, PTHR12498, 1 hit |
Pfami | View protein in Pfam PF14736, N_Asn_amidohyd, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
Q96AB6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPLLVEGRRV RLPQSAGDLV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ
60 70 80 90 100
QRELAVTSPK DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGTDTKA
110 120 130 140 150
EVPLIMNSIK SFSDHAQCGR LEVHLVGGFS DDRQLSQKLT HQLLSEFDRQ
160 170 180 190 200
EDDIHLVTLC VTELNDREEN ENHFPVIYGI AVNIKTAEIY RASFQDRGPE
210 220 230 240 250
EQLRAARTLA GGPMISIYDA ETEQLRIGPY SWTPFPHVDF WLHQDDKQIL
260 270 280 290 300
ENLSTSPLAE PPHFVEHIRS TLMFLKKHPS PAHTLFSGNK ALLYKKNEDG
310
LWEKISSPGS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087X0T5 | A0A087X0T5_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 205 | Annotation score: | ||
H3BNJ5 | H3BNJ5_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 223 | Annotation score: | ||
H3BPN7 | H3BPN7_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 146 | Annotation score: | ||
H3BMX8 | H3BMX8_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 18 | Annotation score: | ||
H3BR97 | H3BR97_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 27 | Annotation score: | ||
H3BU50 | H3BU50_HUMAN | Protein N-terminal asparagine amido... | NTAN1 | 45 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | E → D in AAP97215 (Ref. 1) Curated | 1 | |
Sequence conflicti | 14 | Q → R in AAP97215 (Ref. 1) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051244 | 283 | H → N. Corresponds to variant dbSNP:rs1136001Ensembl. | 1 | |
Natural variantiVAR_051245 | 287 | S → P. Corresponds to variant dbSNP:rs1135999Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092440 mRNA Translation: AAP97215.1 BC017336 mRNA Translation: AAH17336.1 |
CCDSi | CCDS10558.1 |
RefSeqi | NP_001257695.1, NM_001270766.1 NP_001257696.1, NM_001270767.1 NP_775745.1, NM_173474.3 |
Genome annotation databases
Ensembli | ENST00000287706; ENSP00000287706; ENSG00000157045 ENST00000620176; ENSP00000484380; ENSG00000275779 |
GeneIDi | 123803 |
KEGGi | hsa:123803 |
UCSCi | uc002ddd.5, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092440 mRNA Translation: AAP97215.1 BC017336 mRNA Translation: AAH17336.1 |
CCDSi | CCDS10558.1 |
RefSeqi | NP_001257695.1, NM_001270766.1 NP_001257696.1, NM_001270767.1 NP_775745.1, NM_173474.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6A0E | X-ray | 1.95 | A/B | 1-310 | [»] | |
6A0F | X-ray | 2.38 | A/B | 1-310 | [»] | |
6A0H | X-ray | 3.19 | A/B | 1-310 | [»] | |
6A0I | X-ray | 2.00 | A/B | 1-310 | [»] | |
SMRi | Q96AB6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125835, 3 interactors |
IntActi | Q96AB6, 2 interactors |
STRINGi | 9606.ENSP00000287706 |
PTM databases
iPTMneti | Q96AB6 |
PhosphoSitePlusi | Q96AB6 |
Polymorphism and mutation databases
BioMutai | NTAN1 |
DMDMi | 37082118 |
Proteomic databases
EPDi | Q96AB6 |
jPOSTi | Q96AB6 |
MassIVEi | Q96AB6 |
MaxQBi | Q96AB6 |
PaxDbi | Q96AB6 |
PeptideAtlasi | Q96AB6 |
PRIDEi | Q96AB6 |
ProteomicsDBi | 75948 |
Protocols and materials databases
Antibodypediai | 24973, 89 antibodies |
DNASUi | 123803 |
Genome annotation databases
Ensembli | ENST00000287706; ENSP00000287706; ENSG00000157045 ENST00000620176; ENSP00000484380; ENSG00000275779 |
GeneIDi | 123803 |
KEGGi | hsa:123803 |
UCSCi | uc002ddd.5, human |
Organism-specific databases
CTDi | 123803 |
DisGeNETi | 123803 |
EuPathDBi | HostDB:ENSG00000157045.8 |
GeneCardsi | NTAN1 |
HGNCi | HGNC:29909, NTAN1 |
HPAi | ENSG00000157045, Low tissue specificity |
MIMi | 615367, gene |
neXtProti | NX_Q96AB6 |
OpenTargetsi | ENSG00000157045 |
PharmGKBi | PA134863573 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSQW, Eukaryota |
GeneTreei | ENSGT00390000016730 |
HOGENOMi | CLU_077981_1_0_1 |
InParanoidi | Q96AB6 |
OMAi | VCLAHCD |
OrthoDBi | 804210at2759 |
PhylomeDBi | Q96AB6 |
TreeFami | TF325597 |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000157045-MONOMER |
PathwayCommonsi | Q96AB6 |
Miscellaneous databases
BioGRID-ORCSi | 123803, 6 hits in 846 CRISPR screens |
ChiTaRSi | NTAN1, human |
GenomeRNAii | 123803 |
Pharosi | Q96AB6, Tbio |
PROi | PR:Q96AB6 |
RNActi | Q96AB6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000157045, Expressed in testis and 239 other tissues |
ExpressionAtlasi | Q96AB6, baseline and differential |
Genevisiblei | Q96AB6, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026750, NTAN1 |
PANTHERi | PTHR12498, PTHR12498, 1 hit |
Pfami | View protein in Pfam PF14736, N_Asn_amidohyd, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NTAN1_HUMAN | |
Accessioni | Q96AB6Primary (citable) accession number: Q96AB6 Secondary accession number(s): Q7Z4Z0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations