UniProtKB - Q96A72 (MGN2_HUMAN)
Protein
Protein mago nashi homolog 2
Gene
MAGOHB
Organism
Homo sapiens (Human)
Status
Functioni
Required for pre-mRNA splicing as component of the spliceosome (PubMed:28502770, PubMed:29301961, PubMed:30705154). Plays a redundant role with MAGOH in the exon junction complex and in the nonsense-mediated decay (NMD) pathway (PubMed:23917022).4 Publications
GO - Molecular functioni
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA 3'-end processing Source: Reactome
- mRNA export from nucleus Source: Reactome
- mRNA splicing, via spliceosome Source: UniProtKB
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: HGNC
- RNA export from nucleus Source: Reactome
- RNA splicing Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing, mRNA transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q96A72 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: Protein mago nashi homolog 2 |
Gene namesi | Name:MAGOHB Synonyms:MAGOH2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000111196.9 |
HGNCi | HGNC:25504, MAGOHB |
neXtProti | NX_Q96A72 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Nucleus
- catalytic step 2 spliceosome Source: GO_Central
- exon-exon junction complex Source: HGNC
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- U2-type catalytic step 1 spliceosome Source: UniProtKB
- U2-type precatalytic spliceosome Source: UniProtKB
Other locations
- neuronal cell body Source: Ensembl
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 55110 |
OpenTargetsi | ENSG00000111196 |
PharmGKBi | PA162394899 |
Miscellaneous databases
Pharosi | Q96A72, Tdark |
Polymorphism and mutation databases
BioMutai | MAGOHB |
DMDMi | 74731095 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000174146 | 2 – 148 | Protein mago nashi homolog 2Add BLAST | 147 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q96A72 |
jPOSTi | Q96A72 |
MassIVEi | Q96A72 |
MaxQBi | Q96A72 |
PaxDbi | Q96A72 |
PeptideAtlasi | Q96A72 |
PRIDEi | Q96A72 |
ProteomicsDBi | 75929 |
TopDownProteomicsi | Q96A72 |
PTM databases
iPTMneti | Q96A72 |
PhosphoSitePlusi | Q96A72 |
SwissPalmi | Q96A72 |
Expressioni
Tissue specificityi
Ubiquitous.
Gene expression databases
Bgeei | ENSG00000111196, Expressed in calcaneal tendon and 185 other tissues |
ExpressionAtlasi | Q96A72, baseline and differential |
Genevisiblei | Q96A72, HS |
Organism-specific databases
HPAi | ENSG00000111196, Low tissue specificity |
Interactioni
Subunit structurei
Component of the pre-catalytic, catalytic and post-catalytic spliceosome complexes (PubMed:28502770, PubMed:29301961, PubMed:30705154). Heterodimer with RBM8A. Core component of the mRNA splicing-dependent exon junction complex (EJC); the core complex contains CASC3, EIF4A3, MAGOH or MAGOHB, and RBM8A (PubMed:23917022).
4 PublicationsBinary interactionsi
Q96A72
Protein-protein interaction databases
BioGRIDi | 120420, 101 interactors |
ComplexPortali | CPX-680, Exon junction subcomplex MAGOHB-Y14 CPX-682, Exon junction core complex, MAGOHB variant |
IntActi | Q96A72, 83 interactors |
MINTi | Q96A72 |
STRINGi | 9606.ENSP00000319240 |
Miscellaneous databases
RNActi | Q96A72, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96A72 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the mago nashi family.Curated
Phylogenomic databases
eggNOGi | KOG3392, Eukaryota |
GeneTreei | ENSGT00390000003156 |
HOGENOMi | CLU_109497_1_1_1 |
InParanoidi | Q96A72 |
OMAi | EDDTNWP |
OrthoDBi | 1373136at2759 |
PhylomeDBi | Q96A72 |
TreeFami | TF300128 |
Family and domain databases
CDDi | cd11295, Mago_nashi, 1 hit |
Gene3Di | 3.30.1560.10, 1 hit |
InterProi | View protein in InterPro IPR004023, Mago_nashi IPR036605, Mago_nashi_sf |
PANTHERi | PTHR12638, PTHR12638, 1 hit |
Pfami | View protein in Pfam PF02792, Mago_nashi, 1 hit |
SUPFAMi | SSF89817, SSF89817, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q96A72-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVASDFYLR YYVGHKGKFG HEFLEFEFRP DGKLRYANNS NYKNDVMIRK
60 70 80 90 100
EAYVHKSVME ELKRIIDDSE ITKEDDALWP PPDRVGRQEL EIVIGDEHIS
110 120 130 140
FTTSKIGSLI DVNQSKDPEG LRVFYYLVQD LKCLVFSLIG LHFKIKPI
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H3U9 | F5H3U9_HUMAN | Protein mago nashi homolog 2 | MAGOHB | 44 | Annotation score: | ||
F5H6P7 | F5H6P7_HUMAN | Protein mago nashi homolog 2 | MAGOHB | 102 | Annotation score: | ||
F5H6N1 | F5H6N1_HUMAN | Protein mago nashi homolog 2 | MAGOHB | 90 | Annotation score: | ||
F5H124 | F5H124_HUMAN | Protein mago nashi homolog 2 | MAGOHB | 81 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036431 | 119 | E → K in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF165518 mRNA Translation: AAF86648.1 AK001154 mRNA Translation: BAA91522.1 AK022720 mRNA Translation: BAB14202.1 BC010905 mRNA Translation: AAH10905.1 |
CCDSi | CCDS8628.1 |
RefSeqi | NP_001306914.1, NM_001319985.1 NP_060518.1, NM_018048.4 |
Genome annotation databases
Ensembli | ENST00000320756; ENSP00000319240; ENSG00000111196 |
GeneIDi | 55110 |
KEGGi | hsa:55110 |
UCSCi | uc001qyq.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF165518 mRNA Translation: AAF86648.1 AK001154 mRNA Translation: BAA91522.1 AK022720 mRNA Translation: BAB14202.1 BC010905 mRNA Translation: AAH10905.1 |
CCDSi | CCDS8628.1 |
RefSeqi | NP_001306914.1, NM_001319985.1 NP_060518.1, NM_018048.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5XJC | electron microscopy | 3.60 | v | 1-148 | [»] | |
5YZG | electron microscopy | 4.10 | v | 1-148 | [»] | |
6ICZ | electron microscopy | 3.00 | v | 1-148 | [»] | |
6QDV | electron microscopy | 3.30 | 9 | 5-145 | [»] | |
SMRi | Q96A72 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120420, 101 interactors |
ComplexPortali | CPX-680, Exon junction subcomplex MAGOHB-Y14 CPX-682, Exon junction core complex, MAGOHB variant |
IntActi | Q96A72, 83 interactors |
MINTi | Q96A72 |
STRINGi | 9606.ENSP00000319240 |
PTM databases
iPTMneti | Q96A72 |
PhosphoSitePlusi | Q96A72 |
SwissPalmi | Q96A72 |
Polymorphism and mutation databases
BioMutai | MAGOHB |
DMDMi | 74731095 |
Proteomic databases
EPDi | Q96A72 |
jPOSTi | Q96A72 |
MassIVEi | Q96A72 |
MaxQBi | Q96A72 |
PaxDbi | Q96A72 |
PeptideAtlasi | Q96A72 |
PRIDEi | Q96A72 |
ProteomicsDBi | 75929 |
TopDownProteomicsi | Q96A72 |
Protocols and materials databases
Antibodypediai | 23361, 60 antibodies |
Genome annotation databases
Ensembli | ENST00000320756; ENSP00000319240; ENSG00000111196 |
GeneIDi | 55110 |
KEGGi | hsa:55110 |
UCSCi | uc001qyq.3, human |
Organism-specific databases
CTDi | 55110 |
DisGeNETi | 55110 |
EuPathDBi | HostDB:ENSG00000111196.9 |
GeneCardsi | MAGOHB |
HGNCi | HGNC:25504, MAGOHB |
HPAi | ENSG00000111196, Low tissue specificity |
neXtProti | NX_Q96A72 |
OpenTargetsi | ENSG00000111196 |
PharmGKBi | PA162394899 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3392, Eukaryota |
GeneTreei | ENSGT00390000003156 |
HOGENOMi | CLU_109497_1_1_1 |
InParanoidi | Q96A72 |
OMAi | EDDTNWP |
OrthoDBi | 1373136at2759 |
PhylomeDBi | Q96A72 |
TreeFami | TF300128 |
Enzyme and pathway databases
PathwayCommonsi | Q96A72 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 55110, 36 hits in 847 CRISPR screens |
ChiTaRSi | MAGOHB, human |
GenomeRNAii | 55110 |
Pharosi | Q96A72, Tdark |
PROi | PR:Q96A72 |
RNActi | Q96A72, protein |
Gene expression databases
Bgeei | ENSG00000111196, Expressed in calcaneal tendon and 185 other tissues |
ExpressionAtlasi | Q96A72, baseline and differential |
Genevisiblei | Q96A72, HS |
Family and domain databases
CDDi | cd11295, Mago_nashi, 1 hit |
Gene3Di | 3.30.1560.10, 1 hit |
InterProi | View protein in InterPro IPR004023, Mago_nashi IPR036605, Mago_nashi_sf |
PANTHERi | PTHR12638, PTHR12638, 1 hit |
Pfami | View protein in Pfam PF02792, Mago_nashi, 1 hit |
SUPFAMi | SSF89817, SSF89817, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MGN2_HUMAN | |
Accessioni | Q96A72Primary (citable) accession number: Q96A72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations