UniProtKB - Q96A61 (TRI52_HUMAN)
Protein
E3 ubiquitin-protein ligase TRIM52
Gene
TRIM52
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase (PubMed:27667714). Positively regulates the NF-kappa-B signaling pathway (PubMed:27667714, PubMed:28073078).2 Publications
(Microbial infection) Exhibits antiviral activity against Japanese encephalitis virus (JEV). Ubiquitinates the viral non-structural protein 2 (NS2A) and targets it for proteasome-mediated degradation.1 Publication
Miscellaneous
Arose via a partial duplication of the TRIM41 gene, followed by independent loss or pseudogenization of TRIM52 in multiple mammalian and primate lineages.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 62 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 43 | |
Zinc fingeri | 222 – 263 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- ubiquitin protein ligase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- defense response to virus Source: UniProtKB
- positive regulation of DNA-binding transcription factor activity Source: GO_Central
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- protein autoubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Antiviral defense, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q96A61 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase TRIM52 (EC:2.3.2.271 Publication)Alternative name(s): RING finger protein 102 Tripartite motif-containing protein 52 |
Gene namesi | Name:TRIM52 Synonyms:RNF102 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19024, TRIM52 |
neXtProti | NX_Q96A61 |
VEuPathDBi | HostDB:ENSG00000183718.5 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nuclear body Source: GO_Central
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 84851 |
OpenTargetsi | ENSG00000183718 |
PharmGKBi | PA134943216 |
Miscellaneous databases
Pharosi | Q96A61, Tdark |
Genetic variation databases
BioMutai | TRIM52 |
DMDMi | 47606198 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056280 | 1 – 297 | E3 ubiquitin-protein ligase TRIM52Add BLAST | 297 |
Post-translational modificationi
Autoubiquitinated (PubMed:27667714). Polyubiquitinated (PubMed:20596523, PubMed:27667714). Undergoes extremely rapid proteolytic degradation by the proteasome (PubMed:31133683).3 Publications
Keywords - PTMi
Ubl conjugationProteomic databases
MassIVEi | Q96A61 |
PaxDbi | Q96A61 |
PeptideAtlasi | Q96A61 |
PRIDEi | Q96A61 |
ProteomicsDBi | 75922 |
PTM databases
iPTMneti | Q96A61 |
Expressioni
Inductioni
Up-regulated by Golgi stress-inducing agents such nigericin, trichostatin, tetoposide, campothecin and brefeldin A (PubMed:31133683). Up-regulated by IL1B/interleukin-1 beta and TNFA/TNF-alpha (PubMed:28073078).2 Publications
Gene expression databases
Bgeei | ENSG00000183718, Expressed in sural nerve and 220 other tissues |
ExpressionAtlasi | Q96A61, baseline and differential |
Genevisiblei | Q96A61, HS |
Organism-specific databases
HPAi | ENSG00000183718, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with Japanese encephalitis virus non-structural protein 2 (NS2A); mediates the ubiquitination of NS2A, targeting it for proteasome-mediated degradation.
1 PublicationProtein-protein interaction databases
BioGRIDi | 124301, 7 interactors |
IntActi | Q96A61, 8 interactors |
STRINGi | 9606.ENSP00000483005 |
Miscellaneous databases
RNActi | Q96A61, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 72 – 167 | Important for rapid proteolytic degradation by the proteasome1 PublicationAdd BLAST | 96 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 51 – 164 | Glu-richAdd BLAST | 114 |
Domaini
The RING-type zinc finger domain is essential for its E3 ubiquitin-protein ligase activity, ability to positively regulate the NF-kappa-B signaling pathway and its antiviral activity.2 Publications
Sequence similaritiesi
Belongs to the TRIM/RBCC family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 62 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 43 | |
Zinc fingeri | 222 – 263 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000154294 |
HOGENOMi | CLU_013137_6_2_1 |
InParanoidi | Q96A61 |
OMAi | SIHSKAY |
OrthoDBi | 423686at2759 |
PhylomeDBi | Q96A61 |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00643, zf-B_box, 1 hit |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q96A61-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGYATTPSP MQTLQEEAVC AICLDYFKDP VSISCGHNFC RGCVTQLWSK
60 70 80 90 100
EDEEDQNEEE DEWEEEEDEE AVGAMDGWDG SIREVLYRGN ADEELFQDQD
110 120 130 140 150
DDELWLGDSG ITNWDNVDYM WDEEEEEEEE DQDYYLGGLR PDLRIDVYRE
160 170 180 190 200
EEILEAYDED EDEELYPDIH PPPSLPLPGQ FTCPQCRKSF TRRSFRPNLQ
210 220 230 240 250
LANMVQIIRQ MCPTPYRGNR SNDQGMCFKH QEALKLFCEV DKEAICVVCR
260 270 280 290
ESRSHKQHSV LPLEEVVQEY QEIKLETTLV GILQIEQESI HSKAYNQ
Sequence cautioni
The sequence BAD92480 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 130 | Missing in BAD92480 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060452 | 272 – 286 | EIKLE…ILQIE → VREMRECGGMKGVER in isoform 2. Add BLAST | 15 | |
Alternative sequenceiVSP_060453 | 287 – 297 | Missing in isoform 2. Add BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JX896146 mRNA Translation: AGA17664.1 AK054802 mRNA Translation: BAB70809.1 AB209243 mRNA Translation: BAD92480.1 Different initiation. AC008443 Genomic DNA No translation available. CH471165 Genomic DNA Translation: EAW53704.1 BC007372 mRNA Translation: AAH07372.1 |
CCDSi | CCDS4467.1 [Q96A61-1] |
RefSeqi | NP_116154.1, NM_032765.3 [Q96A61-1] |
Genome annotation databases
Ensembli | ENST00000611618; ENSP00000483005; ENSG00000183718 [Q96A61-1] |
GeneIDi | 84851 |
KEGGi | hsa:84851 |
UCSCi | uc003mnp.4, human [Q96A61-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JX896146 mRNA Translation: AGA17664.1 AK054802 mRNA Translation: BAB70809.1 AB209243 mRNA Translation: BAD92480.1 Different initiation. AC008443 Genomic DNA No translation available. CH471165 Genomic DNA Translation: EAW53704.1 BC007372 mRNA Translation: AAH07372.1 |
CCDSi | CCDS4467.1 [Q96A61-1] |
RefSeqi | NP_116154.1, NM_032765.3 [Q96A61-1] |
3D structure databases
SMRi | Q96A61 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 124301, 7 interactors |
IntActi | Q96A61, 8 interactors |
STRINGi | 9606.ENSP00000483005 |
PTM databases
iPTMneti | Q96A61 |
Genetic variation databases
BioMutai | TRIM52 |
DMDMi | 47606198 |
Proteomic databases
MassIVEi | Q96A61 |
PaxDbi | Q96A61 |
PeptideAtlasi | Q96A61 |
PRIDEi | Q96A61 |
ProteomicsDBi | 75922 |
Protocols and materials databases
Antibodypediai | 29763, 45 antibodies |
DNASUi | 84851 |
Genome annotation databases
Ensembli | ENST00000611618; ENSP00000483005; ENSG00000183718 [Q96A61-1] |
GeneIDi | 84851 |
KEGGi | hsa:84851 |
UCSCi | uc003mnp.4, human [Q96A61-1] |
Organism-specific databases
CTDi | 84851 |
DisGeNETi | 84851 |
GeneCardsi | TRIM52 |
HGNCi | HGNC:19024, TRIM52 |
HPAi | ENSG00000183718, Low tissue specificity |
neXtProti | NX_Q96A61 |
OpenTargetsi | ENSG00000183718 |
PharmGKBi | PA134943216 |
VEuPathDBi | HostDB:ENSG00000183718.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000154294 |
HOGENOMi | CLU_013137_6_2_1 |
InParanoidi | Q96A61 |
OMAi | SIHSKAY |
OrthoDBi | 423686at2759 |
PhylomeDBi | Q96A61 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q96A61 |
Miscellaneous databases
BioGRID-ORCSi | 84851, 20 hits in 870 CRISPR screens |
ChiTaRSi | TRIM52, human |
GenomeRNAii | 84851 |
Pharosi | Q96A61, Tdark |
PROi | PR:Q96A61 |
RNActi | Q96A61, protein |
Gene expression databases
Bgeei | ENSG00000183718, Expressed in sural nerve and 220 other tissues |
ExpressionAtlasi | Q96A61, baseline and differential |
Genevisiblei | Q96A61, HS |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00643, zf-B_box, 1 hit |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI52_HUMAN | |
Accessioni | Q96A61Primary (citable) accession number: Q96A61 Secondary accession number(s): L0CQ38, Q59G66 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM