UniProtKB - Q96A46 (MFRN2_HUMAN)
Mitoferrin-2
SLC25A28
Functioni
Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (By similarity).
By similarityGO - Molecular functioni
- iron ion transmembrane transporter activity Source: GO_Central
GO - Biological processi
- iron import into the mitochondrion Source: GO_Central
- iron ion homeostasis Source: UniProtKB-KW
Keywordsi
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron |
Enzyme and pathway databases
PathwayCommonsi | Q96A46 |
Reactomei | R-HSA-1362409, Mitochondrial iron-sulfur cluster biogenesis |
SignaLinki | Q96A46 |
Protein family/group databases
TCDBi | 2.A.29.5.8, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitoferrin-2Alternative name(s): Mitochondrial RNA-splicing protein 3/4 homolog Short name: MRS3/4 Short name: hMRS3/4 Mitochondrial iron transporter 2 Solute carrier family 25 member 28 |
Gene namesi | Name:SLC25A28 Synonyms:MFRN2 ORF Names:NPD016 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23472, SLC25A28 |
MIMi | 609767, gene |
neXtProti | NX_Q96A46 |
VEuPathDBi | HostDB:ENSG00000155287 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Isoform 1 and isoform 2 are both localized in the mitochondrion.
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 72 – 91 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Transmembranei | 133 – 152 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Transmembranei | 170 – 189 | Helical; Name=3Sequence analysisAdd BLAST | 20 | |
Transmembranei | 227 – 246 | Helical; Name=4Sequence analysisAdd BLAST | 20 | |
Transmembranei | 261 – 280 | Helical; Name=5Sequence analysisAdd BLAST | 20 | |
Transmembranei | 327 – 346 | Helical; Name=6Sequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Organism-specific databases
DisGeNETi | 81894 |
OpenTargetsi | ENSG00000155287 |
PharmGKBi | PA134867966 |
Miscellaneous databases
Pharosi | Q96A46, Tbio |
Genetic variation databases
BioMutai | SLC25A28 |
DMDMi | 74751734 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235255 | 1 – 364 | Mitoferrin-2Add BLAST | 364 |
Proteomic databases
EPDi | Q96A46 |
jPOSTi | Q96A46 |
MassIVEi | Q96A46 |
MaxQBi | Q96A46 |
PaxDbi | Q96A46 |
PeptideAtlasi | Q96A46 |
PRIDEi | Q96A46 |
ProteomicsDBi | 75908 [Q96A46-1] 75909 [Q96A46-2] 75910 [Q96A46-3] |
PTM databases
iPTMneti | Q96A46 |
PhosphoSitePlusi | Q96A46 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000155287, Expressed in urinary bladder and 227 other tissues |
ExpressionAtlasi | Q96A46, baseline and differential |
Genevisiblei | Q96A46, HS |
Organism-specific databases
HPAi | ENSG00000155287, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 123626, 32 interactors |
IntActi | Q96A46, 4 interactors |
MINTi | Q96A46 |
STRINGi | 9606.ENSP00000359526 |
Miscellaneous databases
RNActi | Q96A46, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 70 – 158 | Solcar 1Add BLAST | 89 | |
Repeati | 168 – 252 | Solcar 2Add BLAST | 85 | |
Repeati | 259 – 352 | Solcar 3Add BLAST | 94 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 28 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 40 – 60 | DisorderedSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0760, Eukaryota |
GeneTreei | ENSGT00940000157049 |
HOGENOMi | CLU_015166_3_1_1 |
InParanoidi | Q96A46 |
OMAi | KVMNPSQ |
OrthoDBi | 1056670at2759 |
PhylomeDBi | Q96A46 |
TreeFami | TF314118 |
Family and domain databases
Gene3Di | 1.50.40.10, 2 hits |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELEGRGAGG VAGGPAAGPG RSPGESALLD GWLQRGVGRG AGGGEAGACR
60 70 80 90 100
PPVRQDPDSG PDYEALPAGA TVTTHMVAGA VAGILEHCVM YPIDCVKTRM
110 120 130 140 150
QSLQPDPAAR YRNVLEALWR IIRTEGLWRP MRGLNVTATG AGPAHALYFA
160 170 180 190 200
CYEKLKKTLS DVIHPGGNSH IANGAAGCVA TLLHDAAMNP AEVVKQRMQM
210 220 230 240 250
YNSPYHRVTD CVRAVWQNEG AGAFYRSYTT QLTMNVPFQA IHFMTYEFLQ
260 270 280 290 300
EHFNPQRRYN PSSHVLSGAC AGAVAAAATT PLDVCKTLLN TQESLALNSH
310 320 330 340 350
ITGHITGMAS AFRTVYQVGG VTAYFRGVQA RVIYQIPSTA IAWSVYEFFK
360
YLITKRQEEW RAGK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX6RFA8 | X6RFA8_HUMAN | Mitoferrin-2 | SLC25A28 | 225 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018413 | 1 – 188 | Missing in isoform 3. 2 PublicationsAdd BLAST | 188 | |
Alternative sequenceiVSP_018414 | 1 – 187 | Missing in isoform 2. 1 PublicationAdd BLAST | 187 | |
Alternative sequenceiVSP_018415 | 189 – 192 | NPAE → MALL in isoform 3. 2 Publications | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327402 mRNA Translation: AAK49519.1 AF327403 mRNA Translation: AAK49520.1 AJ303077 mRNA Translation: CAC27996.1 AJ303078 mRNA Translation: CAC27997.1 AF267854 mRNA Translation: AAG44723.1 AL831943 mRNA Translation: CAH10774.1 AL353719 Genomic DNA No translation available. BC047312 mRNA Translation: AAH47312.1 Different initiation. BC058937 mRNA Translation: AAH58937.1 BC076399 mRNA Translation: AAH76399.1 BC094821 mRNA Translation: AAH94821.1 |
CCDSi | CCDS41559.1 [Q96A46-1] |
RefSeqi | NP_112489.3, NM_031212.3 [Q96A46-1] |
Genome annotation databases
Ensembli | ENST00000370495.6; ENSP00000359526.4; ENSG00000155287.11 |
GeneIDi | 81894 |
KEGGi | hsa:81894 |
MANE-Selecti | ENST00000370495.6; ENSP00000359526.4; NM_031212.4; NP_112489.3 |
UCSCi | uc001kpx.3, human [Q96A46-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327402 mRNA Translation: AAK49519.1 AF327403 mRNA Translation: AAK49520.1 AJ303077 mRNA Translation: CAC27996.1 AJ303078 mRNA Translation: CAC27997.1 AF267854 mRNA Translation: AAG44723.1 AL831943 mRNA Translation: CAH10774.1 AL353719 Genomic DNA No translation available. BC047312 mRNA Translation: AAH47312.1 Different initiation. BC058937 mRNA Translation: AAH58937.1 BC076399 mRNA Translation: AAH76399.1 BC094821 mRNA Translation: AAH94821.1 |
CCDSi | CCDS41559.1 [Q96A46-1] |
RefSeqi | NP_112489.3, NM_031212.3 [Q96A46-1] |
3D structure databases
AlphaFoldDBi | Q96A46 |
SMRi | Q96A46 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123626, 32 interactors |
IntActi | Q96A46, 4 interactors |
MINTi | Q96A46 |
STRINGi | 9606.ENSP00000359526 |
Protein family/group databases
TCDBi | 2.A.29.5.8, the mitochondrial carrier (mc) family |
PTM databases
iPTMneti | Q96A46 |
PhosphoSitePlusi | Q96A46 |
Genetic variation databases
BioMutai | SLC25A28 |
DMDMi | 74751734 |
Proteomic databases
EPDi | Q96A46 |
jPOSTi | Q96A46 |
MassIVEi | Q96A46 |
MaxQBi | Q96A46 |
PaxDbi | Q96A46 |
PeptideAtlasi | Q96A46 |
PRIDEi | Q96A46 |
ProteomicsDBi | 75908 [Q96A46-1] 75909 [Q96A46-2] 75910 [Q96A46-3] |
Protocols and materials databases
Antibodypediai | 31086, 139 antibodies from 19 providers |
DNASUi | 81894 |
Genome annotation databases
Ensembli | ENST00000370495.6; ENSP00000359526.4; ENSG00000155287.11 |
GeneIDi | 81894 |
KEGGi | hsa:81894 |
MANE-Selecti | ENST00000370495.6; ENSP00000359526.4; NM_031212.4; NP_112489.3 |
UCSCi | uc001kpx.3, human [Q96A46-1] |
Organism-specific databases
CTDi | 81894 |
DisGeNETi | 81894 |
GeneCardsi | SLC25A28 |
HGNCi | HGNC:23472, SLC25A28 |
HPAi | ENSG00000155287, Low tissue specificity |
MIMi | 609767, gene |
neXtProti | NX_Q96A46 |
OpenTargetsi | ENSG00000155287 |
PharmGKBi | PA134867966 |
VEuPathDBi | HostDB:ENSG00000155287 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0760, Eukaryota |
GeneTreei | ENSGT00940000157049 |
HOGENOMi | CLU_015166_3_1_1 |
InParanoidi | Q96A46 |
OMAi | KVMNPSQ |
OrthoDBi | 1056670at2759 |
PhylomeDBi | Q96A46 |
TreeFami | TF314118 |
Enzyme and pathway databases
PathwayCommonsi | Q96A46 |
Reactomei | R-HSA-1362409, Mitochondrial iron-sulfur cluster biogenesis |
SignaLinki | Q96A46 |
Miscellaneous databases
BioGRID-ORCSi | 81894, 157 hits in 1082 CRISPR screens |
ChiTaRSi | SLC25A28, human |
GenomeRNAii | 81894 |
Pharosi | Q96A46, Tbio |
PROi | PR:Q96A46 |
RNActi | Q96A46, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000155287, Expressed in urinary bladder and 227 other tissues |
ExpressionAtlasi | Q96A46, baseline and differential |
Genevisiblei | Q96A46, HS |
Family and domain databases
Gene3Di | 1.50.40.10, 2 hits |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | MFRN2_HUMAN | |
Accessioni | Q96A46Primary (citable) accession number: Q96A46 Secondary accession number(s): Q4VBZ0 Q9H2J3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families