UniProtKB - Q96A44 (SPSB4_HUMAN)
SPRY domain-containing SOCS box protein 4
SPSB4
Functioni
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- ubiquitin ligase-substrate adaptor activity Source: UniProtKB
GO - Biological processi
- intracellular signal transduction Source: InterPro
- positive regulation of protein polyubiquitination Source: UniProtKB
- post-translational protein modification Source: Reactome
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein ubiquitination Source: UniProtKB
- regulation of circadian rhythm Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Biological process | Biological rhythms, Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q96A44 |
Reactomei | R-HSA-8951664, Neddylation R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: SPRY domain-containing SOCS box protein 4Short name: SSB-4 |
Gene namesi | Name:SPSB4 Synonyms:SSB4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30630, SPSB4 |
MIMi | 611660, gene |
neXtProti | NX_Q96A44 |
VEuPathDBi | HostDB:ENSG00000175093.4 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Other locations
- SCF ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 92369 |
OpenTargetsi | ENSG00000175093 |
PharmGKBi | PA142670874 |
Miscellaneous databases
Pharosi | Q96A44, Tdark |
Genetic variation databases
BioMutai | SPSB4 |
DMDMi | 74731085 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000238477 | 1 – 273 | SPRY domain-containing SOCS box protein 4Add BLAST | 273 |
Proteomic databases
MassIVEi | Q96A44 |
PaxDbi | Q96A44 |
PeptideAtlasi | Q96A44 |
PRIDEi | Q96A44 |
ProteomicsDBi | 75907 |
PTM databases
iPTMneti | Q96A44 |
PhosphoSitePlusi | Q96A44 |
Expressioni
Gene expression databases
Bgeei | ENSG00000175093, Expressed in testis and 122 other tissues |
ExpressionAtlasi | Q96A44, baseline and differential |
Genevisiblei | Q96A44, HS |
Organism-specific databases
HPAi | ENSG00000175093, Tissue enriched (pancreas) |
Interactioni
Subunit structurei
Component of the probable ECS(SPSB4) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB4 (PubMed:15601820).
Interacts with CUL5; RNF7; ELOB and ELOC (PubMed:15601820).
Interacts with MET (PubMed:15713673).
Interacts (via B30.2/SPRY domain) with PAWR; this interaction occurs in association with the Elongin BC complex (PubMed:20561531).
Interacts with NOS2 (PubMed:21199876).
Interacts with EPHB2 (PubMed:28931592).
5 PublicationsBinary interactionsi
Hide detailsQ96A44
With | #Exp. | IntAct |
---|---|---|
ELOC [Q15369] | 2 | EBI-2323233,EBI-301231 |
VHL - isoform 2 [P40337-2] | 3 | EBI-2323233,EBI-12157263 |
Protein-protein interaction databases
BioGRIDi | 124941, 11 interactors |
ELMi | Q96A44 |
IntActi | Q96A44, 7 interactors |
STRINGi | 9606.ENSP00000311609 |
Miscellaneous databases
RNActi | Q96A44, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q96A44 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q96A44 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 233 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 200 | |
Domaini | 234 – 273 | SOCS boxPROSITE-ProRule annotationAdd BLAST | 40 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3953, Eukaryota |
GeneTreei | ENSGT01010000222472 |
HOGENOMi | CLU_046756_0_1_1 |
InParanoidi | Q96A44 |
OMAi | DLCRRCI |
OrthoDBi | 938259at2759 |
PhylomeDBi | Q96A44 |
TreeFami | TF312822 |
Family and domain databases
Gene3Di | 2.60.120.920, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR001496, SOCS_box IPR036036, SOCS_box-like_dom_sf IPR003877, SPRY_dom |
Pfami | View protein in Pfam PF07525, SOCS_box, 1 hit PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00969, SOCS_box, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF158235, SSF158235, 1 hit SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50225, SOCS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGQKLSGSLK SVEVREPALR PAKRELRGAE PGRPARLDQL LDMPAAGLAV
60 70 80 90 100
QLRHAWNPED RSLNVFVKDD DRLTFHRHPV AQSTDGIRGK VGHARGLHAW
110 120 130 140 150
QINWPARQRG THAVVGVATA RAPLHSVGYT ALVGSDAESW GWDLGRSRLY
160 170 180 190 200
HDGKNQPGVA YPAFLGPDEA FALPDSLLVV LDMDEGTLSF IVDGQYLGVA
210 220 230 240 250
FRGLKGKKLY PVVSAVWGHC EVTMRYINGL DPEPLPLMDL CRRSIRSALG
260 270
RQRLQDISSL PLPQSLKNYL QYQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0Y8T2 | H0Y8T2_HUMAN | SPRY domain-containing SOCS box pro... | SPSB4 | 90 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF403029 mRNA Translation: AAL57348.1 AK056367 mRNA Translation: BAB71165.1 BC008324 mRNA Translation: AAH08324.1 |
CCDSi | CCDS3115.1 |
RefSeqi | NP_543138.1, NM_080862.2 |
Genome annotation databases
Ensembli | ENST00000310546; ENSP00000311609; ENSG00000175093 |
GeneIDi | 92369 |
KEGGi | hsa:92369 |
UCSCi | uc003ett.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF403029 mRNA Translation: AAL57348.1 AK056367 mRNA Translation: BAB71165.1 BC008324 mRNA Translation: AAH08324.1 |
CCDSi | CCDS3115.1 |
RefSeqi | NP_543138.1, NM_080862.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2V24 | X-ray | 2.20 | A | 33-233 | [»] | |
6DN7 | X-ray | 1.40 | A/C | 28-233 | [»] | |
6DN8 | X-ray | 1.75 | A/C/E | 28-233 | [»] | |
SMRi | Q96A44 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124941, 11 interactors |
ELMi | Q96A44 |
IntActi | Q96A44, 7 interactors |
STRINGi | 9606.ENSP00000311609 |
PTM databases
iPTMneti | Q96A44 |
PhosphoSitePlusi | Q96A44 |
Genetic variation databases
BioMutai | SPSB4 |
DMDMi | 74731085 |
Proteomic databases
MassIVEi | Q96A44 |
PaxDbi | Q96A44 |
PeptideAtlasi | Q96A44 |
PRIDEi | Q96A44 |
ProteomicsDBi | 75907 |
Protocols and materials databases
Antibodypediai | 77081, 11 antibodies |
DNASUi | 92369 |
Genome annotation databases
Ensembli | ENST00000310546; ENSP00000311609; ENSG00000175093 |
GeneIDi | 92369 |
KEGGi | hsa:92369 |
UCSCi | uc003ett.4, human |
Organism-specific databases
CTDi | 92369 |
DisGeNETi | 92369 |
GeneCardsi | SPSB4 |
HGNCi | HGNC:30630, SPSB4 |
HPAi | ENSG00000175093, Tissue enriched (pancreas) |
MIMi | 611660, gene |
neXtProti | NX_Q96A44 |
OpenTargetsi | ENSG00000175093 |
PharmGKBi | PA142670874 |
VEuPathDBi | HostDB:ENSG00000175093.4 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3953, Eukaryota |
GeneTreei | ENSGT01010000222472 |
HOGENOMi | CLU_046756_0_1_1 |
InParanoidi | Q96A44 |
OMAi | DLCRRCI |
OrthoDBi | 938259at2759 |
PhylomeDBi | Q96A44 |
TreeFami | TF312822 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q96A44 |
Reactomei | R-HSA-8951664, Neddylation R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 92369, 7 hits in 982 CRISPR screens |
ChiTaRSi | SPSB4, human |
EvolutionaryTracei | Q96A44 |
GenomeRNAii | 92369 |
Pharosi | Q96A44, Tdark |
PROi | PR:Q96A44 |
RNActi | Q96A44, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000175093, Expressed in testis and 122 other tissues |
ExpressionAtlasi | Q96A44, baseline and differential |
Genevisiblei | Q96A44, HS |
Family and domain databases
Gene3Di | 2.60.120.920, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR001496, SOCS_box IPR036036, SOCS_box-like_dom_sf IPR003877, SPRY_dom |
Pfami | View protein in Pfam PF07525, SOCS_box, 1 hit PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00969, SOCS_box, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF158235, SSF158235, 1 hit SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50225, SOCS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPSB4_HUMAN | |
Accessioni | Q96A44Primary (citable) accession number: Q96A44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families