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Protein

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6

Gene

ST6GALNAC6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-2,6-sialyltransferase involved in the synthesis of alpha-series gangliosides. Has activity toward GD1a, GT1b and GM1b. Has no activity toward glycoproteins. Responsible for the biosynthesis of DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) in normal and malignant kidney. Participates in the synthesis of disialyl Lewis a (Le(a)).2 Publications

Miscellaneous

The carbohydrate antigen disialyl Lewis a, which is at least partly synthesized by ST6GALNAC6, is a normal counterpart of sialyl Lewis a, better known as CA19-9, an antigen widely used as a serum marker for diagnosis of cancers in the digestive track. Disialyl Lewis a is predominantly expressed in non-malignant epithelial cells of the digestive organs, while sialyl Lewis a is preferentially expressed in cancers. Disialyl Lewis a in normal epithelial cells serves as a ligand for immunosuppressive receptors, such as SIGLEC7 and SIGLEC9, expressed on resident monocytes/macrophages and maintains immunological homeostasis of mucosal membranes in digestive organs. Sialyl Lewis a, as well as its positional isomer sialyl Lewis x, serves as a ligand for vascular cell adhesion molecule E-selectin and facilitates hematogenous metastasis through mediating adhesion of circulating cancer cells to vascular endothelium (PubMed:17760270).1 Publication

Kineticsi

  1. KM=0.33 mM for GM1b1 Publication
  2. KM=0.46 mM for sialyl-Lc41 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.99.7 2681
    ReactomeiR-HSA-4085001 Sialic acid metabolism

    Protein family/group databases

    CAZyiGT29 Glycosyltransferase Family 29

    Chemistry databases

    SwissLipidsiSLP:000001366

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (EC:2.4.99.-)
    Alternative name(s):
    GalNAc alpha-2,6-sialyltransferase VI
    ST6GalNAc VI
    Short name:
    ST6GalNAcVI
    Short name:
    hST6GalNAc VI
    Sialyltransferase 7F
    Short name:
    SIAT7-F
    Gene namesi
    Name:ST6GALNAC6
    Synonyms:SIAT7F
    ORF Names:UNQ708/PRO1359
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 9

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000160408.14
    HGNCiHGNC:23364 ST6GALNAC6
    MIMi610135 gene
    neXtProtiNX_Q969X2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 43CytoplasmicSequence analysisAdd BLAST43
    Transmembranei44 – 64Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini65 – 333LumenalSequence analysisAdd BLAST269

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi30815
    OpenTargetsiENSG00000160408
    ENSG00000257524
    PharmGKBiPA134891331

    Polymorphism and mutation databases

    DMDMi74751728

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003147951 – 333Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6Add BLAST333

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi98N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi108 ↔ 256By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ969X2
    PeptideAtlasiQ969X2
    PRIDEiQ969X2
    ProteomicsDBi75868
    75869 [Q969X2-2]

    PTM databases

    iPTMnetiQ969X2
    PhosphoSitePlusiQ969X2

    Expressioni

    Tissue specificityi

    Expressed in kidney, in proximal tubule epithelial cells. Expressed in colon cell lines.2 Publications

    Inductioni

    Down-regulated in renal cancers.

    Gene expression databases

    BgeeiENSG00000160408 Expressed in 167 organ(s), highest expression level in prefrontal cortex
    CleanExiHS_ST6GALNAC6
    ExpressionAtlasiQ969X2 baseline and differential
    GenevisibleiQ969X2 HS

    Organism-specific databases

    HPAiHPA018890

    Interactioni

    Protein-protein interaction databases

    BioGridi119039, 3 interactors
    IntActiQ969X2, 4 interactors
    STRINGi9606.ENSP00000291839

    Structurei

    3D structure databases

    ProteinModelPortaliQ969X2
    SMRiQ969X2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi30 – 33Poly-Arg4
    Compositional biasi112 – 115Poly-Ser4

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2692 Eukaryota
    ENOG410XT8P LUCA
    GeneTreeiENSGT00550000074444
    HOGENOMiHOG000293316
    HOVERGENiHBG058710
    InParanoidiQ969X2
    KOiK03376
    OMAiPNMTAYV
    OrthoDBiEOG091G0H9S
    PhylomeDBiQ969X2
    TreeFamiTF323961

    Family and domain databases

    Gene3Di3.90.1480.20, 1 hit
    InterProiView protein in InterPro
    IPR001675 Glyco_trans_29
    IPR038578 GT29-like_sf
    IPR012163 Sialyl_trans
    PfamiView protein in Pfam
    PF00777 Glyco_transf_29, 1 hit
    PIRSFiPIRSF005557 Sialyl_trans, 1 hit

    Sequences (3+)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q969X2-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MACSRPPSQC EPTSLPPGPP AGRRHLPLSR RRREMSSNKE QRSAVFVILF
    60 70 80 90 100
    ALITILILYS SNSANEVFHY GSLRGRSRRP VNLKKWSITD GYVPILGNKT
    110 120 130 140 150
    LPSRCHQCVI VSSSSHLLGT KLGPEIERAE CTIRMNDAPT TGYSADVGNK
    160 170 180 190 200
    TTYRVVAHSS VFRVLRRPQE FVNRTPETVF IFWGPPSKMQ KPQGSLVRVI
    210 220 230 240 250
    QRAGLVFPNM EAYAVSPGRM RQFDDLFRGE TGKDREKSHS WLSTGWFTMV
    260 270 280 290 300
    IAVELCDHVH VYGMVPPNYC SQRPRLQRMP YHYYEPKGPD ECVTYIQNEH
    310 320 330
    SRKGNHHRFI TEKRVFSSWA QLYGITFSHP SWT
    Length:333
    Mass (Da):38,068
    Last modified:December 1, 2001 - v1
    Checksum:i5DB6FFA7D7A707C0
    GO
    Isoform 2 (identifier: Q969X2-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-34: Missing.

    Show »
    Length:299
    Mass (Da):34,291
    Checksum:iF9DAFF04008A0C5F
    GO
    Isoform 3 (identifier: Q969X2-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         272-333: QRPRLQRMPY...GITFSHPSWT → GPASSACPTT...AGVSPSQSGP

    Show »
    Length:374
    Mass (Da):40,781
    Checksum:i04DCE408DBED314E
    GO

    Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    X6RH99X6RH99_HUMAN
    Alpha-N-acetylgalactosaminide alpha...
    ST6GALNAC6
    200Annotation score:
    F6SGE3F6SGE3_HUMAN
    Alpha-N-acetylgalactosaminide alpha...
    ST6GALNAC6
    86Annotation score:

    Sequence cautioni

    The sequence EAW87712 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti73L → M in BC036102 (PubMed:15489334).Curated1
    Sequence conflicti174R → Q in BC036102 (PubMed:15489334).Curated1
    Sequence conflicti228R → Q in BC036102 (PubMed:15489334).Curated1
    Sequence conflicti249M → T in BAB14715 (PubMed:12975309).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0303591 – 34Missing in isoform 2. 5 PublicationsAdd BLAST34
    Alternative sequenceiVSP_055722272 – 333QRPRL…HPSWT → GPASSACPTTTTSPRGRTNV SPTSRMSTVARATTTASSPR KGSSHRGPSCMASPSPTPPG PRPPSLWDLRRVRGEAASAQ PLGQGPSSGQSRLAGVSPSQ SGP in isoform 3. 1 PublicationAdd BLAST62

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ507293 mRNA Translation: CAD45373.1
    AB035173 mRNA Translation: BAA87035.1
    AY358672 mRNA Translation: AAQ89035.1
    AK023900 mRNA Translation: BAB14715.1
    AK057100 mRNA Translation: BAG51863.1
    CR457318 mRNA Translation: CAG33599.1
    AL157935 Genomic DNA No translation available.
    CH471090 Genomic DNA Translation: EAW87711.1
    CH471090 Genomic DNA Translation: EAW87712.1 Sequence problems.
    CH471090 Genomic DNA Translation: EAW87714.1
    BC006564 mRNA Translation: AAH06564.1
    BC007802 mRNA Translation: AAH07802.1
    BC016299 mRNA Translation: AAH16299.1
    BC036102 mRNA No translation available.
    CCDSiCCDS6882.1 [Q969X2-1]
    CCDS69668.1 [Q969X2-2]
    CCDS69669.1 [Q969X2-3]
    RefSeqiNP_001273928.1, NM_001286999.1 [Q969X2-3]
    NP_001273929.1, NM_001287000.1 [Q969X2-2]
    NP_001273930.1, NM_001287001.1 [Q969X2-2]
    NP_001273931.1, NM_001287002.1 [Q969X2-2]
    NP_001273932.1, NM_001287003.1
    NP_038471.2, NM_013443.4 [Q969X2-1]
    XP_011516912.1, XM_011518610.2 [Q969X2-1]
    XP_011516913.1, XM_011518611.2 [Q969X2-2]
    XP_016870148.1, XM_017014659.1 [Q969X2-3]
    XP_016870154.1, XM_017014665.1 [Q969X2-2]
    XP_016870155.1, XM_017014666.1 [Q969X2-2]
    XP_016870156.1, XM_017014667.1 [Q969X2-2]
    UniGeneiHs.109672

    Genome annotation databases

    EnsembliENST00000291839; ENSP00000291839; ENSG00000160408 [Q969X2-1]
    ENST00000373141; ENSP00000362234; ENSG00000160408 [Q969X2-2]
    ENST00000373142; ENSP00000362235; ENSG00000160408 [Q969X2-3]
    ENST00000373144; ENSP00000362237; ENSG00000160408 [Q969X2-2]
    ENST00000373146; ENSP00000362239; ENSG00000160408 [Q969X2-1]
    ENST00000622357; ENSP00000477575; ENSG00000160408 [Q969X2-2]
    GeneIDi30815
    KEGGihsa:30815
    UCSCiuc004bsn.3 human [Q969X2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    ST6GalNAc VI

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ507293 mRNA Translation: CAD45373.1
    AB035173 mRNA Translation: BAA87035.1
    AY358672 mRNA Translation: AAQ89035.1
    AK023900 mRNA Translation: BAB14715.1
    AK057100 mRNA Translation: BAG51863.1
    CR457318 mRNA Translation: CAG33599.1
    AL157935 Genomic DNA No translation available.
    CH471090 Genomic DNA Translation: EAW87711.1
    CH471090 Genomic DNA Translation: EAW87712.1 Sequence problems.
    CH471090 Genomic DNA Translation: EAW87714.1
    BC006564 mRNA Translation: AAH06564.1
    BC007802 mRNA Translation: AAH07802.1
    BC016299 mRNA Translation: AAH16299.1
    BC036102 mRNA No translation available.
    CCDSiCCDS6882.1 [Q969X2-1]
    CCDS69668.1 [Q969X2-2]
    CCDS69669.1 [Q969X2-3]
    RefSeqiNP_001273928.1, NM_001286999.1 [Q969X2-3]
    NP_001273929.1, NM_001287000.1 [Q969X2-2]
    NP_001273930.1, NM_001287001.1 [Q969X2-2]
    NP_001273931.1, NM_001287002.1 [Q969X2-2]
    NP_001273932.1, NM_001287003.1
    NP_038471.2, NM_013443.4 [Q969X2-1]
    XP_011516912.1, XM_011518610.2 [Q969X2-1]
    XP_011516913.1, XM_011518611.2 [Q969X2-2]
    XP_016870148.1, XM_017014659.1 [Q969X2-3]
    XP_016870154.1, XM_017014665.1 [Q969X2-2]
    XP_016870155.1, XM_017014666.1 [Q969X2-2]
    XP_016870156.1, XM_017014667.1 [Q969X2-2]
    UniGeneiHs.109672

    3D structure databases

    ProteinModelPortaliQ969X2
    SMRiQ969X2
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi119039, 3 interactors
    IntActiQ969X2, 4 interactors
    STRINGi9606.ENSP00000291839

    Chemistry databases

    SwissLipidsiSLP:000001366

    Protein family/group databases

    CAZyiGT29 Glycosyltransferase Family 29

    PTM databases

    iPTMnetiQ969X2
    PhosphoSitePlusiQ969X2

    Polymorphism and mutation databases

    DMDMi74751728

    Proteomic databases

    PaxDbiQ969X2
    PeptideAtlasiQ969X2
    PRIDEiQ969X2
    ProteomicsDBi75868
    75869 [Q969X2-2]

    Protocols and materials databases

    DNASUi30815
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000291839; ENSP00000291839; ENSG00000160408 [Q969X2-1]
    ENST00000373141; ENSP00000362234; ENSG00000160408 [Q969X2-2]
    ENST00000373142; ENSP00000362235; ENSG00000160408 [Q969X2-3]
    ENST00000373144; ENSP00000362237; ENSG00000160408 [Q969X2-2]
    ENST00000373146; ENSP00000362239; ENSG00000160408 [Q969X2-1]
    ENST00000622357; ENSP00000477575; ENSG00000160408 [Q969X2-2]
    GeneIDi30815
    KEGGihsa:30815
    UCSCiuc004bsn.3 human [Q969X2-1]

    Organism-specific databases

    CTDi30815
    DisGeNETi30815
    EuPathDBiHostDB:ENSG00000160408.14
    GeneCardsiST6GALNAC6
    H-InvDBiHIX0008413
    HGNCiHGNC:23364 ST6GALNAC6
    HPAiHPA018890
    MIMi610135 gene
    neXtProtiNX_Q969X2
    OpenTargetsiENSG00000160408
    ENSG00000257524
    PharmGKBiPA134891331
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2692 Eukaryota
    ENOG410XT8P LUCA
    GeneTreeiENSGT00550000074444
    HOGENOMiHOG000293316
    HOVERGENiHBG058710
    InParanoidiQ969X2
    KOiK03376
    OMAiPNMTAYV
    OrthoDBiEOG091G0H9S
    PhylomeDBiQ969X2
    TreeFamiTF323961

    Enzyme and pathway databases

    BRENDAi2.4.99.7 2681
    ReactomeiR-HSA-4085001 Sialic acid metabolism

    Miscellaneous databases

    ChiTaRSiST6GALNAC6 human
    GeneWikiiST6GALNAC6
    GenomeRNAii30815
    PROiPR:Q969X2
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000160408 Expressed in 167 organ(s), highest expression level in prefrontal cortex
    CleanExiHS_ST6GALNAC6
    ExpressionAtlasiQ969X2 baseline and differential
    GenevisibleiQ969X2 HS

    Family and domain databases

    Gene3Di3.90.1480.20, 1 hit
    InterProiView protein in InterPro
    IPR001675 Glyco_trans_29
    IPR038578 GT29-like_sf
    IPR012163 Sialyl_trans
    PfamiView protein in Pfam
    PF00777 Glyco_transf_29, 1 hit
    PIRSFiPIRSF005557 Sialyl_trans, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSIA7F_HUMAN
    AccessioniPrimary (citable) accession number: Q969X2
    Secondary accession number(s): B3KQ01
    , Q5T9C4, Q5T9C5, Q9H8A2, Q9ULB8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: December 1, 2001
    Last modified: November 7, 2018
    This is version 134 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
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