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Protein

Myocardin-related transcription factor A

Gene

MRTFA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex. Activity is also regulated by filamentous actin (F-actin) in the nucleus.By similarity

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actin monomer binding Source: UniProtKB
  • leucine zipper domain binding Source: BHF-UCL
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3899300 SUMOylation of transcription cofactors
R-HSA-5663220 RHO GTPases Activate Formins
SIGNORiQ969V6

Names & Taxonomyi

Protein namesi
Recommended name:
Myocardin-related transcription factor ACurated
Short name:
MRTF-ACurated
Alternative name(s):
MKL/myocardin-like protein 11 Publication
Megakaryoblastic leukemia 1 protein1 Publication
Megakaryocytic acute leukemia proteinBy similarity
Gene namesi
Name:MRTFAImported
Synonyms:KIAA14381 Publication, MALBy similarity, MKL11 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000196588.14
HGNCiHGNC:14334 MRTFA
MIMi606078 gene
neXtProtiNX_Q969V6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving MRTFA may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15 (PubMed:11431691, PubMed:11344311). Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MRTFA chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene (PubMed:11431691, PubMed:11344311).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei3 – 4Breakpoint for translocation to form RBM15-MRTFA1 Publication2

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi57591
MalaCardsiMKL1
OpenTargetsiENSG00000196588
Orphaneti402023 Megakaryoblastic acute myeloid leukemia with t(1;22)(p13;q13)
PharmGKBiPA30827

Chemistry databases

DrugBankiDB08080 LATRUNCULIN B

Polymorphism and mutation databases

BioMutaiMKL1
DMDMi32363202

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001266251 – 931Myocardin-related transcription factor AAdd BLAST931

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineCombined sources1
Modified residuei124PhosphoserineBy similarity1
Modified residuei139PhosphoserineBy similarity1
Modified residuei156PhosphoserineCombined sources1
Modified residuei305PhosphothreonineCombined sources1
Modified residuei310PhosphoserineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei313PhosphothreonineBy similarity1
Modified residuei317PhosphoserineBy similarity1
Modified residuei320PhosphoserineBy similarity1
Modified residuei333PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1
Modified residuei446PhosphoserineBy similarity1
Modified residuei447PhosphothreonineBy similarity1
Modified residuei449PhosphoserineBy similarity1
Modified residuei450PhosphothreonineCombined sources1
Modified residuei454PhosphoserineCombined sources1
Modified residuei456PhosphothreonineBy similarity1
Modified residuei458PhosphoserineBy similarity1
Modified residuei482PhosphoserineBy similarity1
Modified residuei492PhosphoserineBy similarity1
Modified residuei507PhosphoserineBy similarity1
Modified residuei511PhosphoserineBy similarity1
Modified residuei685PhosphoserineBy similarity1
Modified residuei691PhosphoserineBy similarity1
Modified residuei695PhosphoserineBy similarity1
Modified residuei792PhosphoserineBy similarity1
Modified residuei807PhosphoserineBy similarity1
Modified residuei859PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-6 by Erk inhibits binding of globular actin (G-actin), unmasking the nuclear localization signal (NLS) and promoting nuclear import.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ969V6
MaxQBiQ969V6
PaxDbiQ969V6
PeptideAtlasiQ969V6
PRIDEiQ969V6
ProteomicsDBi75854

PTM databases

iPTMnetiQ969V6
PhosphoSitePlusiQ969V6

Expressioni

Tissue specificityi

Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.1 Publication

Gene expression databases

BgeeiENSG00000196588 Expressed in 205 organ(s), highest expression level in blood
CleanExiHS_MAL
HS_MKL1
ExpressionAtlasiQ969V6 baseline and differential
GenevisibleiQ969V6 HS

Organism-specific databases

HPAiHPA030782

Interactioni

Subunit structurei

Interacts with SRF, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF (PubMed:14565952, PubMed:19350017). Interacts (via RPEL repeats) with globular actin (G-actin), thereby regulating its subcellular location and activity of the complex formed with SRF (PubMed:19350017). Either forms a trivalent (by binding three G-actin monomers) or pentavalent (by binding five G-actin monomers) complex with G-actin (By similarity). Forms a nuclear ternary complex with SCAI and SRF, leading to suppress MRTFA-induced SRF transcriptional activity (PubMed:19350017). Interacts with beta-actin (ACTB); interaction with ACTB prevents interaction with SCAI (By similarity). Interacts with MRTFB (PubMed:14565952).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SRFP118312EBI-493122,EBI-493034

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121642, 24 interactors
IntActiQ969V6, 12 interactors
MINTiQ969V6
STRINGi9606.ENSP00000347847

Structurei

Secondary structure

1931
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ969V6
SMRiQ969V6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ969V6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati24 – 49RPEL 1Add BLAST26
Repeati68 – 93RPEL 2Add BLAST26
Domaini347 – 381SAPPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 256Mediates interaction with SCAI and ACTBBy similarityAdd BLAST256
Regioni6 – 23Intervening spacer sequence 1By similarityAdd BLAST18
Regioni50 – 67Intervening spacer sequence 2By similarityAdd BLAST18

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili515 – 563Sequence analysisAdd BLAST49

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi264 – 286Gln-richAdd BLAST23
Compositional biasi564 – 811Pro-richAdd BLAST248

Domaini

The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.By similarity
The RPEL repeats mediate binding to globular actin (G-actin); each RPEL repeat-binding to one G-actin monomer. In addition, each intervening spacer sequence region can bind one G-actin monomer, to reach a pentavalent complex.By similarity

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IFJX Eukaryota
ENOG41101AM LUCA
GeneTreeiENSGT00530000063195
HOGENOMiHOG000038001
HOVERGENiHBG036493
InParanoidiQ969V6
KOiK22525
PhylomeDBiQ969V6
TreeFamiTF326024

Family and domain databases

Gene3Di1.10.720.30, 1 hit
InterProiView protein in InterPro
IPR029992 MRTF-A
IPR004018 RPEL_repeat
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR22793:SF6 PTHR22793:SF6, 2 hits
PfamiView protein in Pfam
PF02755 RPEL, 2 hits
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00707 RPEL, 2 hits
SM00513 SAP, 1 hit
SUPFAMiSSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS51073 RPEL, 2 hits
PS50800 SAP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

Q969V6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPPLKSPAAF HEQRRSLERA RTEDYLKRKI RSRPERSELV RMHILEETSA
60 70 80 90 100
EPSLQAKQLK LKRARLADDL NEKIAQRPGP MELVEKNILP VESSLKEAII
110 120 130 140 150
VGQVNYPKVA DSSSFDEDSS DALSPEQPAS HESQGSVPSP LEARVSEPLL
160 170 180 190 200
SATSASPTQV VSQLPMGRDS REMLFLAEQP PLPPPPLLPP SLTNGTTIPT
210 220 230 240 250
AKSTPTLIKQ SQPKSASEKS QRSKKAKELK PKVKKLKYHQ YIPPDQKQDR
260 270 280 290 300
GAPPMDSSYA KILQQQQLFL QLQILNQQQQ QHHNYQAILP APPKSAGEAL
310 320 330 340 350
GSSGTPPVRS LSTTNSSSSS GAPGPCGLAR QNSTSLTGKP GALPANLDDM
360 370 380 390 400
KVAELKQELK LRSLPVSGTK TELIERLRAY QDQISPVPGA PKAPAATSIL
410 420 430 440 450
HKAGEVVVAF PAARLSTGPA LVAAGLAPAE VVVATVASSG VVKFGSTGST
460 470 480 490 500
PPVSPTPSER SLLSTGDENS TPGDTFGEMV TSPLTQLTLQ ASPLQILVKE
510 520 530 540 550
EGPRAGSCCL SPGGRAELEG RDKDQMLQEK DKQIEALTRM LRQKQQLVER
560 570 580 590 600
LKLQLEQEKR AQQPAPAPAP LGTPVKQENS FSSCQLSQQP LGPAHPFNPS
610 620 630 640 650
LAAPATNHID PCAVAPGPPS VVVKQEALQP EPEPVPAPQL LLGPQGPSLI
660 670 680 690 700
KGVAPPTLIT DSTGTHLVLT VTNKNADSPG LSSGSPQQPS SQPGSPAPAP
710 720 730 740 750
SAQMDLEHPL QPLFGTPTSL LKKEPPGYEE AMSQQPKQQE NGSSSQQMDD
760 770 780 790 800
LFDILIQSGE ISADFKEPPS LPGKEKPSPK TVCGSPLAAQ PSPSAELPQA
810 820 830 840 850
APPPPGSPSL PGRLEDFLES STGLPLLTSG HDGPEPLSLI DDLHSQMLSS
860 870 880 890 900
TAILDHPPSP MDTSELHFVP EPSSTMGLDL ADGHLDSMDW LELSSGGPVL
910 920 930
SLAPLSTTAP SLFSTDFLDG HDLQLHWDSC L
Length:931
Mass (Da):98,919
Last modified:December 1, 2001 - v1
Checksum:i6EDE5E2C56D89609
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
W0Z7M9W0Z7M9_HUMAN
Mkl1 variant 1 Mkl1_S
MRTFA mkl1
966Annotation score:
B0QY84B0QY84_HUMAN
Phosphatase and actin regulator
MRTFA MKL1, hCG_41119
161Annotation score:
B0QY74B0QY74_HUMAN
Phosphatase and actin regulator
MRTFA
73Annotation score:
B0QY83B0QY83_HUMAN
Myocardin-related transcription fac...
MRTFA
881Annotation score:
E7ER32E7ER32_HUMAN
Myocardin-related transcription fac...
MRTFA
798Annotation score:
A0A087X287A0A087X287_HUMAN
Myocardin-related transcription fac...
MRTFA
799Annotation score:
A0A087WU73A0A087WU73_HUMAN
Myocardin-related transcription fac...
MRTFA
882Annotation score:
Q29R68Q29R68_HUMAN
MKL1 protein
MRTFA MKL1
85Annotation score:

Sequence cautioni

The sequence AAK56920 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA92676 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAC38828 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAC38829 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021409648S → G2 PublicationsCorresponds to variant dbSNP:rs878756Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297257 mRNA Translation: CAC38826.1
AF364035 mRNA Translation: AAK56920.1 Different initiation.
CR456522 mRNA Translation: CAG30408.1
AJ297258 mRNA Translation: CAC38827.1
AF368061 mRNA Translation: AAK54721.1
AJ303089 mRNA Translation: CAC38828.1 Different initiation.
AJ303090 mRNA Translation: CAC38829.1 Different initiation.
AB037859 mRNA Translation: BAA92676.2 Different initiation.
AL022238 Genomic DNA No translation available.
AL713710 mRNA Translation: CAD28507.2
CCDSiCCDS14003.1
RefSeqiNP_001269589.1, NM_001282660.1
NP_001269590.1, NM_001282661.1
NP_065882.1, NM_020831.4
XP_005261751.1, XM_005261694.1
UniGeneiHs.654688

Genome annotation databases

EnsembliENST00000355630; ENSP00000347847; ENSG00000196588
ENST00000407029; ENSP00000385835; ENSG00000196588
GeneIDi57591
KEGGihsa:57591
UCSCiuc003ayv.3 human

Keywords - Coding sequence diversityi

Chromosomal rearrangement, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297257 mRNA Translation: CAC38826.1
AF364035 mRNA Translation: AAK56920.1 Different initiation.
CR456522 mRNA Translation: CAG30408.1
AJ297258 mRNA Translation: CAC38827.1
AF368061 mRNA Translation: AAK54721.1
AJ303089 mRNA Translation: CAC38828.1 Different initiation.
AJ303090 mRNA Translation: CAC38829.1 Different initiation.
AB037859 mRNA Translation: BAA92676.2 Different initiation.
AL022238 Genomic DNA No translation available.
AL713710 mRNA Translation: CAD28507.2
CCDSiCCDS14003.1
RefSeqiNP_001269589.1, NM_001282660.1
NP_001269590.1, NM_001282661.1
NP_065882.1, NM_020831.4
XP_005261751.1, XM_005261694.1
UniGeneiHs.654688

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KVUNMR-A336-396[»]
2KW9NMR-A336-396[»]
ProteinModelPortaliQ969V6
SMRiQ969V6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121642, 24 interactors
IntActiQ969V6, 12 interactors
MINTiQ969V6
STRINGi9606.ENSP00000347847

Chemistry databases

DrugBankiDB08080 LATRUNCULIN B

PTM databases

iPTMnetiQ969V6
PhosphoSitePlusiQ969V6

Polymorphism and mutation databases

BioMutaiMKL1
DMDMi32363202

Proteomic databases

EPDiQ969V6
MaxQBiQ969V6
PaxDbiQ969V6
PeptideAtlasiQ969V6
PRIDEiQ969V6
ProteomicsDBi75854

Protocols and materials databases

DNASUi57591
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355630; ENSP00000347847; ENSG00000196588
ENST00000407029; ENSP00000385835; ENSG00000196588
GeneIDi57591
KEGGihsa:57591
UCSCiuc003ayv.3 human

Organism-specific databases

CTDi57591
DisGeNETi57591
EuPathDBiHostDB:ENSG00000196588.14
GeneCardsiMKL1
HGNCiHGNC:14334 MRTFA
HPAiHPA030782
MalaCardsiMKL1
MIMi606078 gene
neXtProtiNX_Q969V6
OpenTargetsiENSG00000196588
Orphaneti402023 Megakaryoblastic acute myeloid leukemia with t(1;22)(p13;q13)
PharmGKBiPA30827
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFJX Eukaryota
ENOG41101AM LUCA
GeneTreeiENSGT00530000063195
HOGENOMiHOG000038001
HOVERGENiHBG036493
InParanoidiQ969V6
KOiK22525
PhylomeDBiQ969V6
TreeFamiTF326024

Enzyme and pathway databases

ReactomeiR-HSA-3899300 SUMOylation of transcription cofactors
R-HSA-5663220 RHO GTPases Activate Formins
SIGNORiQ969V6

Miscellaneous databases

ChiTaRSiMKL1 human
EvolutionaryTraceiQ969V6
GeneWikiiMKL1
GenomeRNAii57591
PROiPR:Q969V6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196588 Expressed in 205 organ(s), highest expression level in blood
CleanExiHS_MAL
HS_MKL1
ExpressionAtlasiQ969V6 baseline and differential
GenevisibleiQ969V6 HS

Family and domain databases

Gene3Di1.10.720.30, 1 hit
InterProiView protein in InterPro
IPR029992 MRTF-A
IPR004018 RPEL_repeat
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR22793:SF6 PTHR22793:SF6, 2 hits
PfamiView protein in Pfam
PF02755 RPEL, 2 hits
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00707 RPEL, 2 hits
SM00513 SAP, 1 hit
SUPFAMiSSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS51073 RPEL, 2 hits
PS50800 SAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMRTFA_HUMAN
AccessioniPrimary (citable) accession number: Q969V6
Secondary accession number(s): Q8TCL1
, Q96SC5, Q96SC6, Q9P2B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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