Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nicalin

Gene

NCLN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.By similarity

GO - Biological processi

  • protein stabilization Source: UniProtKB
  • regulation of protein complex assembly Source: UniProtKB
  • regulation of protein complex stability Source: UniProtKB
  • regulation of signal transduction Source: InterPro

Protein family/group databases

MEROPSiM28.978

Names & Taxonomyi

Protein namesi
Recommended name:
Nicalin
Alternative name(s):
Nicastrin-like protein
Gene namesi
Name:NCLN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000125912.10
HGNCiHGNC:26923 NCLN
MIMi609156 gene
neXtProtiNX_Q969V3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini43 – 522LumenalSequence analysisAdd BLAST480
Transmembranei523 – 543HelicalSequence analysisAdd BLAST21
Topological domaini544 – 563CytoplasmicSequence analysisAdd BLAST20

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000125912
PharmGKBiPA134898417

Polymorphism and mutation databases

BioMutaiNCLN
DMDMi68052797

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Sequence analysisAdd BLAST42
ChainiPRO_000001968743 – 563NicalinAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi428N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ969V3
MaxQBiQ969V3
PaxDbiQ969V3
PeptideAtlasiQ969V3
PRIDEiQ969V3
ProteomicsDBi75850
75851 [Q969V3-2]

PTM databases

GlyConnecti1566
iPTMnetiQ969V3
PhosphoSitePlusiQ969V3
SwissPalmiQ969V3

Expressioni

Tissue specificityi

Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.1 Publication

Gene expression databases

BgeeiENSG00000125912 Expressed in 160 organ(s), highest expression level in mucosa of transverse colon
ExpressionAtlasiQ969V3 baseline and differential
GenevisibleiQ969V3 HS

Organism-specific databases

HPAiHPA006625

Interactioni

Subunit structurei

Forms a complex with NOMO2 and TMEM147, resulting in a stabilization of the 3 proteins, which are otherwise quickly degraded by the proteasome. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP5-9P263713EBI-10281480,EBI-3958099

Protein-protein interaction databases

BioGridi121253, 33 interactors
IntActiQ969V3, 11 interactors
STRINGi9606.ENSP00000246117

Structurei

3D structure databases

ProteinModelPortaliQ969V3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nicastrin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2526 Eukaryota
ENOG410XQZK LUCA
GeneTreeiENSGT00500000044945
HOVERGENiHBG054932
InParanoidiQ969V3
OMAiRHCVLTR
OrthoDBiEOG091G041D
PhylomeDBiQ969V3
TreeFamiTF105849

Family and domain databases

InterProiView protein in InterPro
IPR016574 Nicalin
IPR007484 Peptidase_M28
PANTHERiPTHR31826 PTHR31826, 1 hit
PfamiView protein in Pfam
PF04389 Peptidase_M28, 1 hit
PIRSFiPIRSF011018 Nicalin, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q969V3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLEEAGEVLE NMLKASCLPL GFIVFLPAVL LLVAPPLPAA DAAHEFTVYR
60 70 80 90 100
MQQYDLQGQP YGTRNAVLNT EARTMAAEVL SRRCVLMRLL DFSYEQYQKA
110 120 130 140 150
LRQSAGAVVI ILPRAMAAVP QDVVRQFMEI EPEMLAMETA VPVYFAVEDE
160 170 180 190 200
ALLSIYKQTQ AASASQGSAS AAEVLLRTAT ANGFQMVTSG VQSKAVSDWL
210 220 230 240 250
IASVEGRLTG LGGEDLPTIV IVAHYDAFGV APWLSLGADS NGSGVSVLLE
260 270 280 290 300
LARLFSRLYT YKRTHAAYNL LFFASGGGKF NYQGTKRWLE DNLDHTDSSL
310 320 330 340 350
LQDNVAFVLC LDTVGRGSSL HLHVSKPPRE GTLQHAFLRE LETVAAHQFP
360 370 380 390 400
EVRFSMVHKR INLAEDVLAW EHERFAIRRL PAFTLSHLES HRDGQRSSIM
410 420 430 440 450
DVRSRVDSKT LTRNTRIIAE ALTRVIYNLT EKGTPPDMPV FTEQMQIQQE
460 470 480 490 500
QLDSVMDWLT NQPRAAQLVD KDSTFLSTLE HHLSRYLKDV KQHHVKADKR
510 520 530 540 550
DPEFVFYDQL KQVMNAYRVK PAVFDLLLAV GIAAYLGMAY VAVQHFSLLY
560
KTVQRLLVKA KTQ
Length:563
Mass (Da):62,974
Last modified:October 25, 2004 - v2
Checksum:i5E74EDF6ABA6F44F
GO
Isoform 2 (identifier: Q969V3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     446-446: Missing.

Show »
Length:562
Mass (Da):62,846
Checksum:iC66C473B8BF9DA6D
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EMW4K7EMW4_HUMAN
Nicalin homolog (Zebrafish), isofor...
NCLN hCG_23630
489Annotation score:
K7ENM2K7ENM2_HUMAN
Nicalin
NCLN
328Annotation score:
K7ELZ9K7ELZ9_HUMAN
Nicalin
NCLN
282Annotation score:
K7EN96K7EN96_HUMAN
Nicalin
NCLN
72Annotation score:
A0A0C4DGP7A0A0C4DGP7_HUMAN
Nicalin
NCLN
350Annotation score:
K7EQ66K7EQ66_HUMAN
Nicalin
NCLN
110Annotation score:

Sequence cautioni

The sequence AAH03076 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH25926 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB66502 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19P → L in CAG38597 (Ref. 6) Curated1
Sequence conflicti124V → A in BAD18812 (PubMed:14702039).Curated1
Sequence conflicti207R → W in CAG38597 (Ref. 6) Curated1
Sequence conflicti387H → L in AAH03076 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050276214E → D. Corresponds to variant dbSNP:rs11671067Ensembl.1
Natural variantiVAR_022552551K → R2 PublicationsCorresponds to variant dbSNP:rs2288949Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013851446Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172848 mRNA Translation: BAD18812.1
AC005331 Genomic DNA Translation: AAC27667.1
AC011547 Genomic DNA No translation available.
CH471139 Genomic DNA Translation: EAW69330.1
CH471139 Genomic DNA Translation: EAW69333.1
BC003076 mRNA Translation: AAH03076.2 Different initiation.
BC007275 mRNA Translation: AAH07275.1
BC008920 mRNA Translation: AAH08920.2
BC010064 mRNA Translation: AAH10064.2
BC013283 mRNA Translation: AAH13283.2
BC019091 mRNA Translation: AAH19091.2
BC025926 mRNA Translation: AAH25926.1 Different initiation.
AL136567 mRNA Translation: CAB66502.1 Different initiation.
CR533566 mRNA Translation: CAG38597.1
AL365369 mRNA Translation: CAB96945.1
CCDSiCCDS32869.1 [Q969V3-1]
RefSeqiNP_001308392.1, NM_001321463.1 [Q969V3-2]
NP_064555.2, NM_020170.3 [Q969V3-1]
UniGeneiHs.657032

Genome annotation databases

EnsembliENST00000246117; ENSP00000246117; ENSG00000125912 [Q969V3-1]
GeneIDi56926
KEGGihsa:56926
UCSCiuc002lxi.4 human [Q969V3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172848 mRNA Translation: BAD18812.1
AC005331 Genomic DNA Translation: AAC27667.1
AC011547 Genomic DNA No translation available.
CH471139 Genomic DNA Translation: EAW69330.1
CH471139 Genomic DNA Translation: EAW69333.1
BC003076 mRNA Translation: AAH03076.2 Different initiation.
BC007275 mRNA Translation: AAH07275.1
BC008920 mRNA Translation: AAH08920.2
BC010064 mRNA Translation: AAH10064.2
BC013283 mRNA Translation: AAH13283.2
BC019091 mRNA Translation: AAH19091.2
BC025926 mRNA Translation: AAH25926.1 Different initiation.
AL136567 mRNA Translation: CAB66502.1 Different initiation.
CR533566 mRNA Translation: CAG38597.1
AL365369 mRNA Translation: CAB96945.1
CCDSiCCDS32869.1 [Q969V3-1]
RefSeqiNP_001308392.1, NM_001321463.1 [Q969V3-2]
NP_064555.2, NM_020170.3 [Q969V3-1]
UniGeneiHs.657032

3D structure databases

ProteinModelPortaliQ969V3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121253, 33 interactors
IntActiQ969V3, 11 interactors
STRINGi9606.ENSP00000246117

Protein family/group databases

MEROPSiM28.978

PTM databases

GlyConnecti1566
iPTMnetiQ969V3
PhosphoSitePlusiQ969V3
SwissPalmiQ969V3

Polymorphism and mutation databases

BioMutaiNCLN
DMDMi68052797

Proteomic databases

EPDiQ969V3
MaxQBiQ969V3
PaxDbiQ969V3
PeptideAtlasiQ969V3
PRIDEiQ969V3
ProteomicsDBi75850
75851 [Q969V3-2]

Protocols and materials databases

DNASUi56926
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000246117; ENSP00000246117; ENSG00000125912 [Q969V3-1]
GeneIDi56926
KEGGihsa:56926
UCSCiuc002lxi.4 human [Q969V3-1]

Organism-specific databases

CTDi56926
EuPathDBiHostDB:ENSG00000125912.10
GeneCardsiNCLN
HGNCiHGNC:26923 NCLN
HPAiHPA006625
MIMi609156 gene
neXtProtiNX_Q969V3
OpenTargetsiENSG00000125912
PharmGKBiPA134898417
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2526 Eukaryota
ENOG410XQZK LUCA
GeneTreeiENSGT00500000044945
HOVERGENiHBG054932
InParanoidiQ969V3
OMAiRHCVLTR
OrthoDBiEOG091G041D
PhylomeDBiQ969V3
TreeFamiTF105849

Miscellaneous databases

ChiTaRSiNCLN human
GeneWikiiNCLN
GenomeRNAii56926
PROiPR:Q969V3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125912 Expressed in 160 organ(s), highest expression level in mucosa of transverse colon
ExpressionAtlasiQ969V3 baseline and differential
GenevisibleiQ969V3 HS

Family and domain databases

InterProiView protein in InterPro
IPR016574 Nicalin
IPR007484 Peptidase_M28
PANTHERiPTHR31826 PTHR31826, 1 hit
PfamiView protein in Pfam
PF04389 Peptidase_M28, 1 hit
PIRSFiPIRSF011018 Nicalin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCLN_HUMAN
AccessioniPrimary (citable) accession number: Q969V3
Secondary accession number(s): D6W613
, O75252, Q6FI60, Q6ZMB7, Q8TAT7, Q96H48, Q96IS7, Q9BQH9, Q9BTX4, Q9NPP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 25, 2004
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again