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Protein

7-methylguanosine phosphate-specific 5'-nucleotidase

Gene

NT5C3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate (PubMed:23223233, PubMed:24603684). The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids (PubMed:23223233). Also has weak activity for CMP (PubMed:23223233, PubMed:24603684). UMP and purine nucleotides are poor substrates (PubMed:23223233).2 Publications

Catalytic activityi

N7-methyl-GMP + H2O = N7-methyl-guanosine + phosphate.1 Publication
CMP + H2O = cytidine + phosphate.1 Publication
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.1 Publication

Kineticsi

kcat is 0.24 sec(-1) with m7GMP. kcat is 7 sec(-1) with CMP. kcat is 0.07 sec(-1) with GMP. kcat is 0.04 sec(-1) with AMP. kcat is 6.2 sec(-1) with UMP.
  1. KM=7.8 µM for m7GMP (at 37 degrees Celsius)1 Publication
  2. KM=8 µM for m7GMP (at 37 degrees Celsius)1 Publication
  3. KM=79 µM for CMP (at 37 degrees Celsius)2 Publications
  4. KM=355 µM for GMP (at 37 degrees Celsius)1 Publication
  5. KM=456 µM for AMP (at 37 degrees Celsius)1 Publication
  6. KM=439 µM for UMP (at 37 degrees Celsius)1 Publication
  1. Vmax=0.41 µmol/min/mg enzyme with m7GMP as substrate1 Publication
  2. Vmax=12 µmol/min/mg enzyme with CMP as substrate1 Publication
  3. Vmax=0.13 µmol/min/mg enzyme with GMP as substrate1 Publication
  4. Vmax=0.07 µmol/min/mg enzyme with AMP as substrate1 Publication
  5. Vmax=10.7 µmol/min/mg enzyme with UMP as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei41NucleophileBy similarity1
Metal bindingi41MagnesiumBy similarity1
Active sitei43Proton donorBy similarity1
Metal bindingi43Magnesium; via carbonyl oxygenBy similarity1
Binding sitei88CMPBy similarity1
Binding sitei88N(7)-methyl-GMPBy similarity1
Binding sitei205SubstrateBy similarity1
Metal bindingi230MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-429958 mRNA decay by 3' to 5' exoribonuclease
SABIO-RKiQ969T7

Names & Taxonomyi

Protein namesi
Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidase1 Publication (EC:3.1.3.911 Publication)
Short name:
7-methylguanosine nucleotidase
Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like protein1 Publication (EC:3.1.3.51 Publication)
Short name:
cN-III-like protein
N(7)-methylguanylate 5'-phosphatase
Gene namesi
Name:NT5C3B
Synonyms:NT5C3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000141698.16
HGNCiHGNC:28300 NT5C3B
neXtProtiNX_Q969T7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000141698

Polymorphism and mutation databases

BioMutaiNT5C3B
DMDMi476007845

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289481 – 3007-methylguanosine phosphate-specific 5'-nucleotidaseAdd BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei256N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ969T7
MaxQBiQ969T7
PaxDbiQ969T7
PeptideAtlasiQ969T7
PRIDEiQ969T7
ProteomicsDBi75844

PTM databases

DEPODiQ969T7
iPTMnetiQ969T7
PhosphoSitePlusiQ969T7

Expressioni

Gene expression databases

BgeeiENSG00000141698 Expressed in 201 organ(s), highest expression level in testis
CleanExiHS_NT5C3L
ExpressionAtlasiQ969T7 baseline and differential

Organism-specific databases

HPAiHPA030786
HPA057095

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MLH1P406923EBI-2932564,EBI-744248

Protein-protein interaction databases

BioGridi125410, 4 interactors
IntActiQ969T7, 1 interactor
STRINGi9606.ENSP00000389948

Structurei

3D structure databases

ProteinModelPortaliQ969T7
SMRiQ969T7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 157Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the pyrimidine 5'-nucleotidase family.Curated

Phylogenomic databases

eggNOGiKOG3128 Eukaryota
ENOG410ZQJ8 LUCA
GeneTreeiENSGT00390000012959
HOGENOMiHOG000244931
HOVERGENiHBG059750
KOiK01081
OMAiKAHDLLC
OrthoDBiEOG091G0BCN
TreeFamiTF314663

Family and domain databases

CDDicd07504 HAD_5NT, 1 hit
Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006434 Pyrimidine_nucleotidase_eu
PfamiView protein in Pfam
PF05822 UMPH-1, 1 hit
SFLDiSFLDG01128 C1.4:_5'-Nucleotidase_Like, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01544 HAD-SF-IE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q969T7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA
60 70 80 90 100
YNGKRCPSSY NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP
110 120 130 140 150
HMVEWWTKAH NLLCQQKIQK FQIAQVVRES NAMLREGYKT FFNTLYHNNI
160 170 180 190 200
PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFNEDG FLQGFKGQLI
210 220 230 240 250
HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG VPGVQNILKI
260 270 280 290 300
GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP
Length:300
Mass (Da):34,389
Last modified:April 3, 2013 - v4
Checksum:i3E221583153381F8
GO
Isoform 2 (identifier: Q969T7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:292
Mass (Da):33,528
Checksum:i8ACC59D5F25E629A
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J758C9J758_HUMAN
7-methylguanosine phosphate-specifi...
NT5C3B
227Annotation score:
E5RH64E5RH64_HUMAN
7-methylguanosine phosphate-specifi...
NT5C3B
200Annotation score:
U3KQB1U3KQB1_HUMAN
7-methylguanosine phosphate-specifi...
NT5C3B
105Annotation score:
E5RJR6E5RJR6_HUMAN
7-methylguanosine phosphate-specifi...
NT5C3B
57Annotation score:
C9IZA4C9IZA4_HUMAN
7-methylguanosine phosphate-specifi...
NT5C3B
74Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_042582209A → V2 PublicationsCorresponds to variant dbSNP:rs1046403Ensembl.1
Natural variantiVAR_042583213S → C2 PublicationsCorresponds to variant dbSNP:rs1046404Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0462971 – 8Missing in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091172 Genomic DNA No translation available.
CH471152 Genomic DNA Translation: EAW60771.1
BC013742 mRNA Translation: AAH13742.2
BC014132 mRNA Translation: AAH14132.2
BC016971 mRNA Translation: AAH16971.2
BC067788 mRNA Translation: AAH67788.1
CCDSiCCDS11410.2 [Q969T7-1]
RefSeqiNP_443167.4, NM_052935.4 [Q969T7-1]
UniGeneiHs.237536

Genome annotation databases

EnsembliENST00000435506; ENSP00000389948; ENSG00000141698 [Q969T7-1]
GeneIDi115024
KEGGihsa:115024
UCSCiuc021txo.2 human [Q969T7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091172 Genomic DNA No translation available.
CH471152 Genomic DNA Translation: EAW60771.1
BC013742 mRNA Translation: AAH13742.2
BC014132 mRNA Translation: AAH14132.2
BC016971 mRNA Translation: AAH16971.2
BC067788 mRNA Translation: AAH67788.1
CCDSiCCDS11410.2 [Q969T7-1]
RefSeqiNP_443167.4, NM_052935.4 [Q969T7-1]
UniGeneiHs.237536

3D structure databases

ProteinModelPortaliQ969T7
SMRiQ969T7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125410, 4 interactors
IntActiQ969T7, 1 interactor
STRINGi9606.ENSP00000389948

PTM databases

DEPODiQ969T7
iPTMnetiQ969T7
PhosphoSitePlusiQ969T7

Polymorphism and mutation databases

BioMutaiNT5C3B
DMDMi476007845

Proteomic databases

EPDiQ969T7
MaxQBiQ969T7
PaxDbiQ969T7
PeptideAtlasiQ969T7
PRIDEiQ969T7
ProteomicsDBi75844

Protocols and materials databases

DNASUi115024
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000435506; ENSP00000389948; ENSG00000141698 [Q969T7-1]
GeneIDi115024
KEGGihsa:115024
UCSCiuc021txo.2 human [Q969T7-1]

Organism-specific databases

CTDi115024
EuPathDBiHostDB:ENSG00000141698.16
GeneCardsiNT5C3B
HGNCiHGNC:28300 NT5C3B
HPAiHPA030786
HPA057095
neXtProtiNX_Q969T7
OpenTargetsiENSG00000141698
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3128 Eukaryota
ENOG410ZQJ8 LUCA
GeneTreeiENSGT00390000012959
HOGENOMiHOG000244931
HOVERGENiHBG059750
KOiK01081
OMAiKAHDLLC
OrthoDBiEOG091G0BCN
TreeFamiTF314663

Enzyme and pathway databases

ReactomeiR-HSA-429958 mRNA decay by 3' to 5' exoribonuclease
SABIO-RKiQ969T7

Miscellaneous databases

GenomeRNAii115024
PROiPR:Q969T7

Gene expression databases

BgeeiENSG00000141698 Expressed in 201 organ(s), highest expression level in testis
CleanExiHS_NT5C3L
ExpressionAtlasiQ969T7 baseline and differential

Family and domain databases

CDDicd07504 HAD_5NT, 1 hit
Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006434 Pyrimidine_nucleotidase_eu
PfamiView protein in Pfam
PF05822 UMPH-1, 1 hit
SFLDiSFLDG01128 C1.4:_5'-Nucleotidase_Like, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01544 HAD-SF-IE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5NT3B_HUMAN
AccessioniPrimary (citable) accession number: Q969T7
Secondary accession number(s): A8MWB9, C9JKC4, Q7L3B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 3, 2013
Last modified: November 7, 2018
This is version 139 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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