Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase I, mitochondrial

Gene

TOP1MT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity1 Publication

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Cofactori

Ca2+1 Publication, Mg2+1 PublicationNote: Divalent metal ions (calcium or magnesium).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei559O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Enzyme and pathway databases

BRENDAi5.99.1.2 2681

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase I, mitochondrial (EC:5.99.1.2)
Short name:
TOP1mt
Gene namesi
Name:TOP1MT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000184428.12
HGNCiHGNC:29787 TOP1MT
MIMi606387 gene
neXtProtiNX_Q969P6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi116447
OpenTargetsiENSG00000184428
PharmGKBiPA134922772

Chemistry databases

ChEMBLiCHEMBL2362989
DrugBankiDB00762 Irinotecan
DB01030 Topotecan

Polymorphism and mutation databases

BioMutaiTOP1MT
DMDMi20140694

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50Mitochondrion1 PublicationAdd BLAST50
ChainiPRO_000003479751 – 601DNA topoisomerase I, mitochondrialAdd BLAST551

Proteomic databases

EPDiQ969P6
MaxQBiQ969P6
PaxDbiQ969P6
PeptideAtlasiQ969P6
PRIDEiQ969P6
ProteomicsDBi75812

PTM databases

iPTMnetiQ969P6
PhosphoSitePlusiQ969P6
SwissPalmiQ969P6

Expressioni

Tissue specificityi

Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.1 Publication

Gene expression databases

BgeeiENSG00000184428 Expressed in 157 organ(s), highest expression level in testis
CleanExiHS_TOP1MT
ExpressionAtlasiQ969P6 baseline and differential
GenevisibleiQ969P6 HS

Organism-specific databases

HPAiHPA001915
HPA021542

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei152Interaction with DNABy similarity1
Sitei200Interaction with DNABy similarity1
Sitei248Interaction with DNABy similarity1
Sitei279Interaction with DNABy similarity1
Sitei337Interaction with DNABy similarity1
Sitei368Interaction with DNABy similarity1
Sitei410Interaction with DNABy similarity1
Sitei468Interaction with DNABy similarity1
Sitei486Interaction with DNABy similarity1

Protein-protein interaction databases

BioGridi125510, 12 interactors
IntActiQ969P6, 11 interactors
STRINGi9606.ENSP00000328835

Structurei

3D structure databases

ProteinModelPortaliQ969P6
SMRiQ969P6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni261 – 262Interaction with DNABy similarity2
Regioni324 – 329Interaction with DNABy similarity6
Regioni421 – 423Interaction with DNABy similarity3

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0981 Eukaryota
COG3569 LUCA
GeneTreeiENSGT00390000016347
HOGENOMiHOG000105469
HOVERGENiHBG007988
InParanoidiQ969P6
KOiK03163
OMAiVINCSRD
OrthoDBiEOG091G02KT
PhylomeDBiQ969P6
TreeFamiTF105281

Family and domain databases

CDDicd00659 Topo_IB_C, 1 hit
Gene3Di1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 2 hits
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013034 DNA_topo_DNA_db_N_dom1
IPR013030 DNA_topo_DNA_db_N_dom2
IPR001631 TopoI
IPR018521 TopoI_AS
IPR025834 TopoI_C_dom
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR014727 TopoI_cat_a/b-sub_euk
IPR013500 TopoI_cat_euk
IPR008336 TopoI_DNA-bd_euk
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR013499 TopoI_euk
PANTHERiPTHR10290 PTHR10290, 1 hit
PfamiView protein in Pfam
PF14370 Topo_C_assoc, 1 hit
PF01028 Topoisom_I, 1 hit
PF02919 Topoisom_I_N, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SMARTiView protein in SMART
SM00435 TOPEUc, 1 hit
SUPFAMiSSF56349 SSF56349, 2 hits
SSF56741 SSF56741, 1 hit
PROSITEiView protein in PROSITE
PS00176 TOPOISOMERASE_I_EUK, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q969P6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRVVRLLRLR AALTLLGEVP RRPASRGVPG SRRTQKGSGA RWEKEKHEDG
60 70 80 90 100
VKWRQLEHKG PYFAPPYEPL PDGVRFFYEG RPVRLSVAAE EVATFYGRML
110 120 130 140 150
DHEYTTKEVF RKNFFNDWRK EMAVEEREVI KSLDKCDFTE IHRYFVDKAA
160 170 180 190 200
ARKVLSREEK QKLKEEAEKL QQEFGYCILD GHQEKIGNFK IEPPGLFRGR
210 220 230 240 250
GDHPKMGMLK RRITPEDVVI NCSRDSKIPE PPAGHQWKEV RSDNTVTWLA
260 270 280 290 300
AWTESVQNSI KYIMLNPCSK LKGETAWQKF ETARRLRGFV DEIRSQYRAD
310 320 330 340 350
WKSREMKTRQ RAVALYFIDK LALRAGNEKE DGEAADTVGC CSLRVEHVQL
360 370 380 390 400
HPEADGCQHV VEFDFLGKDC IRYYNRVPVE KPVYKNLQLF MENKDPRDDL
410 420 430 440 450
FDRLTTTSLN KHLQELMDGL TAKVFRTYNA SITLQEQLRA LTRAEDSIAA
460 470 480 490 500
KILSYNRANR VVAILCNHQR ATPSTFEKSM QNLQTKIQAK KEQVAEARAE
510 520 530 540 550
LRRARAEHKA QGDGKSRSVL EKKRRLLEKL QEQLAQLSVQ ATDKEENKQV
560 570 580 590 600
ALGTSKLNYL DPRISIAWCK RFRVPVEKIY SKTQRERFAW ALAMAGEDFE

F
Length:601
Mass (Da):69,872
Last modified:December 1, 2001 - v1
Checksum:iA012248D4250C675
GO
Isoform 2 (identifier: Q969P6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: Missing.

Note: No experimental confirmation available.
Show »
Length:503
Mass (Da):58,645
Checksum:i7E0138EE2931B903
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RFS0E5RFS0_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
221Annotation score:
E5RIC7E5RIC7_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
376Annotation score:
E5RJ95E5RJ95_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
201Annotation score:
H0YAR3H0YAR3_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
111Annotation score:
E5RGE7E5RGE7_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
134Annotation score:
H0YBR3H0YBR3_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
49Annotation score:
E5RGR2E5RGR2_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
167Annotation score:
H0YC03H0YC03_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
123Annotation score:
E5RJ33E5RJ33_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
33Annotation score:
E5RGR4E5RGR4_HUMAN
DNA topoisomerase I, mitochondrial
TOP1MT
164Annotation score:
There is more potential isoformShow all

Sequence cautioni

The sequence AAH44646 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116N → S in BAH14751 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052593256V → I1 PublicationCorresponds to variant dbSNP:rs11544484Ensembl.1
Natural variantiVAR_021863525R → W1 PublicationCorresponds to variant dbSNP:rs2293925Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0447831 – 98Missing in isoform 2. 1 PublicationAdd BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF349031
, AF349018, AF349019, AF349020, AF349021, AF349022, AF349023, AF349024, AF349025, AF349026, AF349027, AF349028, AF349029, AF349030 Genomic DNA Translation: AAL10791.1
AF349017 mRNA Translation: AAL05624.1
AK316380 mRNA Translation: BAH14751.1
AC087793 Genomic DNA No translation available.
BC044646 mRNA Translation: AAH44646.1 Different initiation.
BC052285 mRNA Translation: AAH52285.1
BC071914 mRNA Translation: AAH71914.1
CCDSiCCDS59115.1 [Q969P6-2]
CCDS6400.1 [Q969P6-1]
RefSeqiNP_001245375.1, NM_001258446.1 [Q969P6-2]
NP_001245376.1, NM_001258447.1 [Q969P6-2]
NP_443195.1, NM_052963.2 [Q969P6-1]
UniGeneiHs.528574
Hs.734085

Genome annotation databases

EnsembliENST00000329245; ENSP00000328835; ENSG00000184428 [Q969P6-1]
ENST00000519148; ENSP00000429169; ENSG00000184428 [Q969P6-2]
ENST00000521193; ENSP00000428369; ENSG00000184428 [Q969P6-2]
ENST00000523676; ENSP00000429181; ENSG00000184428 [Q969P6-2]
GeneIDi116447
KEGGihsa:116447
UCSCiuc003yxz.6 human [Q969P6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF349031
, AF349018, AF349019, AF349020, AF349021, AF349022, AF349023, AF349024, AF349025, AF349026, AF349027, AF349028, AF349029, AF349030 Genomic DNA Translation: AAL10791.1
AF349017 mRNA Translation: AAL05624.1
AK316380 mRNA Translation: BAH14751.1
AC087793 Genomic DNA No translation available.
BC044646 mRNA Translation: AAH44646.1 Different initiation.
BC052285 mRNA Translation: AAH52285.1
BC071914 mRNA Translation: AAH71914.1
CCDSiCCDS59115.1 [Q969P6-2]
CCDS6400.1 [Q969P6-1]
RefSeqiNP_001245375.1, NM_001258446.1 [Q969P6-2]
NP_001245376.1, NM_001258447.1 [Q969P6-2]
NP_443195.1, NM_052963.2 [Q969P6-1]
UniGeneiHs.528574
Hs.734085

3D structure databases

ProteinModelPortaliQ969P6
SMRiQ969P6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125510, 12 interactors
IntActiQ969P6, 11 interactors
STRINGi9606.ENSP00000328835

Chemistry databases

ChEMBLiCHEMBL2362989
DrugBankiDB00762 Irinotecan
DB01030 Topotecan

PTM databases

iPTMnetiQ969P6
PhosphoSitePlusiQ969P6
SwissPalmiQ969P6

Polymorphism and mutation databases

BioMutaiTOP1MT
DMDMi20140694

Proteomic databases

EPDiQ969P6
MaxQBiQ969P6
PaxDbiQ969P6
PeptideAtlasiQ969P6
PRIDEiQ969P6
ProteomicsDBi75812

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329245; ENSP00000328835; ENSG00000184428 [Q969P6-1]
ENST00000519148; ENSP00000429169; ENSG00000184428 [Q969P6-2]
ENST00000521193; ENSP00000428369; ENSG00000184428 [Q969P6-2]
ENST00000523676; ENSP00000429181; ENSG00000184428 [Q969P6-2]
GeneIDi116447
KEGGihsa:116447
UCSCiuc003yxz.6 human [Q969P6-1]

Organism-specific databases

CTDi116447
DisGeNETi116447
EuPathDBiHostDB:ENSG00000184428.12
GeneCardsiTOP1MT
HGNCiHGNC:29787 TOP1MT
HPAiHPA001915
HPA021542
MIMi606387 gene
neXtProtiNX_Q969P6
OpenTargetsiENSG00000184428
PharmGKBiPA134922772
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0981 Eukaryota
COG3569 LUCA
GeneTreeiENSGT00390000016347
HOGENOMiHOG000105469
HOVERGENiHBG007988
InParanoidiQ969P6
KOiK03163
OMAiVINCSRD
OrthoDBiEOG091G02KT
PhylomeDBiQ969P6
TreeFamiTF105281

Enzyme and pathway databases

BRENDAi5.99.1.2 2681

Miscellaneous databases

ChiTaRSiTOP1MT human
GenomeRNAii116447
PROiPR:Q969P6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184428 Expressed in 157 organ(s), highest expression level in testis
CleanExiHS_TOP1MT
ExpressionAtlasiQ969P6 baseline and differential
GenevisibleiQ969P6 HS

Family and domain databases

CDDicd00659 Topo_IB_C, 1 hit
Gene3Di1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 2 hits
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013034 DNA_topo_DNA_db_N_dom1
IPR013030 DNA_topo_DNA_db_N_dom2
IPR001631 TopoI
IPR018521 TopoI_AS
IPR025834 TopoI_C_dom
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR014727 TopoI_cat_a/b-sub_euk
IPR013500 TopoI_cat_euk
IPR008336 TopoI_DNA-bd_euk
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR013499 TopoI_euk
PANTHERiPTHR10290 PTHR10290, 1 hit
PfamiView protein in Pfam
PF14370 Topo_C_assoc, 1 hit
PF01028 Topoisom_I, 1 hit
PF02919 Topoisom_I_N, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SMARTiView protein in SMART
SM00435 TOPEUc, 1 hit
SUPFAMiSSF56349 SSF56349, 2 hits
SSF56741 SSF56741, 1 hit
PROSITEiView protein in PROSITE
PS00176 TOPOISOMERASE_I_EUK, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTOP1M_HUMAN
AccessioniPrimary (citable) accession number: Q969P6
Secondary accession number(s): B7ZAR5
, E7ES89, Q86ST4, Q86V82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again