UniProtKB - Q969P0 (IGSF8_HUMAN)
Protein
Immunoglobulin superfamily member 8
Gene
IGSF8
Organism
Homo sapiens (Human)
Status
Functioni
May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.5 Publications
GO - Biological processi
- cell motility Source: UniProtKB
- nervous system development Source: UniProtKB
- single fertilization Source: UniProtKB
- skeletal muscle tissue development Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q969P0 |
Names & Taxonomyi
Protein namesi | Recommended name: Immunoglobulin superfamily member 8Short name: IgSF8 Alternative name(s): CD81 partner 3 Glu-Trp-Ile EWI motif-containing protein 2 Short name: EWI-2 Keratinocytes-associated transmembrane protein 4 Short name: KCT-4 LIR-D1 Prostaglandin regulatory-like protein Short name: PGRL CD_antigen: CD316 |
Gene namesi | Name:IGSF8 Synonyms:CD81P3, EWI2, KCT4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17813, IGSF8 |
MIMi | 606644, gene |
neXtProti | NX_Q969P0 |
VEuPathDBi | HostDB:ENSG00000162729.13 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass membrane protein
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 579 | ExtracellularSequence analysisAdd BLAST | 552 | |
Transmembranei | 580 – 600 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 601 – 613 | CytoplasmicSequence analysisAdd BLAST | 13 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 93185 |
OpenTargetsi | ENSG00000162729 |
PharmGKBi | PA29767 |
Miscellaneous databases
Pharosi | Q969P0, Tbio |
Genetic variation databases
BioMutai | IGSF8 |
DMDMi | 74762642 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000226247 | 28 – 613 | Immunoglobulin superfamily member 8Add BLAST | 586 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 ↔ 127 | PROSITE-ProRule annotation | ||
Glycosylationi | 50 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 186 ↔ 270 | PROSITE-ProRule annotation | ||
Disulfide bondi | 326 ↔ 406 | PROSITE-ProRule annotation | ||
Glycosylationi | 327 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 462 ↔ 544 | PROSITE-ProRule annotation | ||
Glycosylationi | 463 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 518 | PhosphoserineCombined sources | 1 | |
Lipidationi | 604 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 605 | S-palmitoyl cysteineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | Q969P0 |
jPOSTi | Q969P0 |
MassIVEi | Q969P0 |
MaxQBi | Q969P0 |
PaxDbi | Q969P0 |
PeptideAtlasi | Q969P0 |
PRIDEi | Q969P0 |
ProteomicsDBi | 75806 [Q969P0-1] 75807 [Q969P0-2] 75808 [Q969P0-3] |
2D gel databases
UCD-2DPAGEi | Q969P0 |
PTM databases
GlyConnecti | 1394, 22 N-Linked glycans (2 sites) |
GlyGeni | Q969P0, 3 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q969P0 |
PhosphoSitePlusi | Q969P0 |
SwissPalmi | Q969P0 |
Expressioni
Tissue specificityi
Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.3 Publications
Gene expression databases
Bgeei | ENSG00000162729, Expressed in C1 segment of cervical spinal cord and 182 other tissues |
ExpressionAtlasi | Q969P0, baseline and differential |
Genevisiblei | Q969P0, HS |
Organism-specific databases
HPAi | ENSG00000162729, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Interacts directly with CD82, CD81/tetraspanin-28 and CD9/tetraspanin-29.
Also interacts with integrin alpha-3/beta-1 and integrin alpha-4/beta-1.
4 PublicationsBinary interactionsi
Hide detailsQ969P0
Protein-protein interaction databases
BioGRIDi | 125011, 210 interactors |
IntActi | Q969P0, 28 interactors |
MINTi | Q969P0 |
STRINGi | 9606.ENSP00000357065 |
Miscellaneous databases
RNActi | Q969P0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 149 | Ig-like C2-type 1Add BLAST | 122 | |
Domaini | 162 – 286 | Ig-like C2-type 2Add BLAST | 125 | |
Domaini | 303 – 424 | Ig-like C2-type 3Add BLAST | 122 | |
Domaini | 431 – 560 | Ig-like C2-type 4Add BLAST | 130 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 274 – 276 | EWI motif | 3 |
Domaini
The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.By similarity
The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.By similarity
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QTUT, Eukaryota |
GeneTreei | ENSGT00940000161314 |
HOGENOMi | CLU_005187_2_0_1 |
InParanoidi | Q969P0 |
OMAi | QEHTHLA |
OrthoDBi | 409194at2759 |
PhylomeDBi | Q969P0 |
TreeFami | TF332702 |
Family and domain databases
Gene3Di | 2.60.40.10, 4 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 4 hits |
SUPFAMi | SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q969P0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGALRPTLLP PSLPLLLLLM LGMGCWAREV LVPEGPLYRV AGTAVSISCN
60 70 80 90 100
VTGYEGPAQQ NFEWFLYRPE APDTALGIVS TKDTQFSYAV FKSRVVAGEV
110 120 130 140 150
QVQRLQGDAV VLKIARLQAQ DAGIYECHTP STDTRYLGSY SGKVELRVLP
160 170 180 190 200
DVLQVSAAPP GPRGRQAPTS PPRMTVHEGQ ELALGCLART STQKHTHLAV
210 220 230 240 250
SFGRSVPEAP VGRSTLQEVV GIRSDLAVEA GAPYAERLAA GELRLGKEGT
260 270 280 290 300
DRYRMVVGGA QAGDAGTYHC TAAEWIQDPD GSWAQIAEKR AVLAHVDVQT
310 320 330 340 350
LSSQLAVTVG PGERRIGPGE PLELLCNVSG ALPPAGRHAA YSVGWEMAPA
360 370 380 390 400
GAPGPGRLVA QLDTEGVGSL GPGYEGRHIA MEKVASRTYR LRLEAARPGD
410 420 430 440 450
AGTYRCLAKA YVRGSGTRLR EAASARSRPL PVHVREEGVV LEAVAWLAGG
460 470 480 490 500
TVYRGETASL LCNISVRGGP PGLRLAASWW VERPEDGELS SVPAQLVGGV
510 520 530 540 550
GQDGVAELGV RPGGGPVSVE LVGPRSHRLR LHSLGPEDEG VYHCAPSAWV
560 570 580 590 600
QHADYSWYQA GSARSGPVTV YPYMHALDTL FVPLLVGTGV ALVTGATVLG
610
TITCCFMKRL RKR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9J8Z4 | C9J8Z4_HUMAN | Immunoglobulin superfamily member 8 | IGSF8 | 301 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 303 | S → R in AAM94901 (Ref. 4) Curated | 1 | |
Sequence conflicti | 306 | A → P in AAM94901 (Ref. 4) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017429 | 1 – 241 | Missing in isoform 2. 1 PublicationAdd BLAST | 241 | |
Alternative sequenceiVSP_017430 | 242 – 315 | ELRLG…PGERR → MRGRSWHWAAWRGQAHRSTH TWQCPLGDLCPRHQLGGQLC RKWWESGQTWPWRLELPMLS DWLQGSFVWARKGP in isoform 2. 1 PublicationAdd BLAST | 74 | |
Alternative sequenceiVSP_017431 | 475 – 561 | Missing in isoform 3. 1 PublicationAdd BLAST | 87 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF407274 mRNA Translation: AAL01052.1 AY044845 mRNA Translation: AAK92220.1 AY157579 mRNA Translation: AAO13163.1 AF311906 mRNA Translation: AAM94901.1 AK055843 mRNA Translation: BAB71027.1 BC004108 mRNA Translation: AAH04108.2 BC053881 mRNA Translation: AAH53881.1 |
CCDSi | CCDS1195.1 [Q969P0-1] |
RefSeqi | NP_001193594.1, NM_001206665.2 [Q969P0-1] NP_001307176.1, NM_001320247.1 [Q969P0-1] NP_443100.1, NM_052868.5 [Q969P0-1] XP_016858324.1, XM_017002835.1 [Q969P0-1] XP_016858325.1, XM_017002836.1 [Q969P0-1] XP_016858326.1, XM_017002837.1 [Q969P0-1] |
Genome annotation databases
Ensembli | ENST00000314485; ENSP00000316664; ENSG00000162729 [Q969P0-1] ENST00000368086; ENSP00000357065; ENSG00000162729 [Q969P0-1] ENST00000614243; ENSP00000477565; ENSG00000162729 [Q969P0-1] |
GeneIDi | 93185 |
KEGGi | hsa:93185 |
UCSCi | uc001fuz.4, human [Q969P0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF407274 mRNA Translation: AAL01052.1 AY044845 mRNA Translation: AAK92220.1 AY157579 mRNA Translation: AAO13163.1 AF311906 mRNA Translation: AAM94901.1 AK055843 mRNA Translation: BAB71027.1 BC004108 mRNA Translation: AAH04108.2 BC053881 mRNA Translation: AAH53881.1 |
CCDSi | CCDS1195.1 [Q969P0-1] |
RefSeqi | NP_001193594.1, NM_001206665.2 [Q969P0-1] NP_001307176.1, NM_001320247.1 [Q969P0-1] NP_443100.1, NM_052868.5 [Q969P0-1] XP_016858324.1, XM_017002835.1 [Q969P0-1] XP_016858325.1, XM_017002836.1 [Q969P0-1] XP_016858326.1, XM_017002837.1 [Q969P0-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 125011, 210 interactors |
IntActi | Q969P0, 28 interactors |
MINTi | Q969P0 |
STRINGi | 9606.ENSP00000357065 |
PTM databases
GlyConnecti | 1394, 22 N-Linked glycans (2 sites) |
GlyGeni | Q969P0, 3 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q969P0 |
PhosphoSitePlusi | Q969P0 |
SwissPalmi | Q969P0 |
Genetic variation databases
BioMutai | IGSF8 |
DMDMi | 74762642 |
2D gel databases
UCD-2DPAGEi | Q969P0 |
Proteomic databases
EPDi | Q969P0 |
jPOSTi | Q969P0 |
MassIVEi | Q969P0 |
MaxQBi | Q969P0 |
PaxDbi | Q969P0 |
PeptideAtlasi | Q969P0 |
PRIDEi | Q969P0 |
ProteomicsDBi | 75806 [Q969P0-1] 75807 [Q969P0-2] 75808 [Q969P0-3] |
Protocols and materials databases
Antibodypediai | 2186, 249 antibodies |
Genome annotation databases
Ensembli | ENST00000314485; ENSP00000316664; ENSG00000162729 [Q969P0-1] ENST00000368086; ENSP00000357065; ENSG00000162729 [Q969P0-1] ENST00000614243; ENSP00000477565; ENSG00000162729 [Q969P0-1] |
GeneIDi | 93185 |
KEGGi | hsa:93185 |
UCSCi | uc001fuz.4, human [Q969P0-1] |
Organism-specific databases
CTDi | 93185 |
DisGeNETi | 93185 |
GeneCardsi | IGSF8 |
HGNCi | HGNC:17813, IGSF8 |
HPAi | ENSG00000162729, Tissue enhanced (brain) |
MIMi | 606644, gene |
neXtProti | NX_Q969P0 |
OpenTargetsi | ENSG00000162729 |
PharmGKBi | PA29767 |
VEuPathDBi | HostDB:ENSG00000162729.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTUT, Eukaryota |
GeneTreei | ENSGT00940000161314 |
HOGENOMi | CLU_005187_2_0_1 |
InParanoidi | Q969P0 |
OMAi | QEHTHLA |
OrthoDBi | 409194at2759 |
PhylomeDBi | Q969P0 |
TreeFami | TF332702 |
Enzyme and pathway databases
PathwayCommonsi | Q969P0 |
Miscellaneous databases
BioGRID-ORCSi | 93185, 4 hits in 877 CRISPR screens |
ChiTaRSi | IGSF8, human |
GeneWikii | IGSF8 |
GenomeRNAii | 93185 |
Pharosi | Q969P0, Tbio |
PROi | PR:Q969P0 |
RNActi | Q969P0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000162729, Expressed in C1 segment of cervical spinal cord and 182 other tissues |
ExpressionAtlasi | Q969P0, baseline and differential |
Genevisiblei | Q969P0, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 4 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 4 hits |
SUPFAMi | SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IGSF8_HUMAN | |
Accessioni | Q969P0Primary (citable) accession number: Q969P0 Secondary accession number(s): Q8NG09, Q96DP4, Q9BTG9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 7, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot