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UniProtKB - Q969H4 (CNKR1_HUMAN)
Protein
Connector enhancer of kinase suppressor of ras 1
Gene
CNKSR1
Organism
Homo sapiens (Human)
Status
Functioni
May function as an adapter protein or regulator of Ras signaling pathways.
GO - Molecular functioni
- protein-macromolecule adaptor activity Source: MGI
GO - Biological processi
- Ras protein signal transduction Source: MGI
- Rho protein signal transduction Source: MGI
- transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc
Enzyme and pathway databases
PathwayCommonsi | Q969H4 |
Reactomei | R-HSA-5674135, MAP2K and MAPK activation R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants R-HSA-6802948, Signaling by high-kinase activity BRAF mutants R-HSA-6802952, Signaling by BRAF and RAF1 fusions R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF R-HSA-9649948, Signaling downstream of RAS mutants R-HSA-9656223, Signaling by RAF1 mutants |
SignaLinki | Q969H4 |
SIGNORi | Q969H4 |
Names & Taxonomyi
Protein namesi | Recommended name: Connector enhancer of kinase suppressor of ras 1Short name: Connector enhancer of KSR 1 Alternative name(s): CNK homolog protein 1 Short name: CNK1 Short name: hCNK1 Connector enhancer of KSR-like |
Gene namesi | Name:CNKSR1 Synonyms:CNK1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19700, CNKSR1 |
MIMi | 603272, gene |
neXtProti | NX_Q969H4 |
VEuPathDBi | HostDB:ENSG00000142675 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- Membrane By similarity; Peripheral membrane protein By similarity
Plasma Membrane
- plasma membrane Source: ProtInc
Other locations
- cell cortex Source: MGI
- cell-cell junction Source: ProtInc
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 493 | W → A: No interaction with Rho. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10256 |
OpenTargetsi | ENSG00000142675 |
PharmGKBi | PA134901167 |
Miscellaneous databases
Pharosi | Q969H4, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4296242 |
Genetic variation databases
BioMutai | CNKSR1 |
DMDMi | 50400606 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089969 | 1 – 720 | Connector enhancer of kinase suppressor of ras 1Add BLAST | 720 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 307 | PhosphoserineCombined sources | 1 | |
Modified residuei | 314 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated on tyrosine.
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q969H4 |
MassIVEi | Q969H4 |
MaxQBi | Q969H4 |
PaxDbi | Q969H4 |
PeptideAtlasi | Q969H4 |
PRIDEi | Q969H4 |
ProteomicsDBi | 75763 [Q969H4-1] 75764 [Q969H4-2] |
PTM databases
iPTMneti | Q969H4 |
PhosphoSitePlusi | Q969H4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000142675, Expressed in muscle of leg and 220 other tissues |
ExpressionAtlasi | Q969H4, baseline and differential |
Genevisiblei | Q969H4, HS |
Organism-specific databases
HPAi | ENSG00000142675, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Interacts with RHO and RALGDS.
1 PublicationBinary interactionsi
Q969H4
Protein-protein interaction databases
BioGRIDi | 115550, 25 interactors |
CORUMi | Q969H4 |
IntActi | Q969H4, 16 interactors |
MINTi | Q969H4 |
STRINGi | 9606.ENSP00000363371 |
Miscellaneous databases
RNActi | Q969H4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q969H4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q969H4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 70 | SAMPROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 78 – 164 | CRICPROSITE-ProRule annotationAdd BLAST | 87 | |
Domaini | 196 – 285 | PDZPROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 403 – 502 | PHPROSITE-ProRule annotationAdd BLAST | 100 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 285 – 390 | DisorderedSequence analysisAdd BLAST | 106 | |
Regioni | 504 – 573 | DisorderedSequence analysisAdd BLAST | 70 | |
Regioni | 676 – 720 | DisorderedSequence analysisAdd BLAST | 45 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 615 – 646 | Sequence analysisAdd BLAST | 32 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 298 – 312 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 325 – 339 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 347 – 361 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 535 – 572 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 679 – 695 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Belongs to the CNKSR family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1738, Eukaryota |
GeneTreei | ENSGT00940000159599 |
HOGENOMi | CLU_013414_1_0_1 |
InParanoidi | Q969H4 |
OMAi | WRLNSYD |
PhylomeDBi | Q969H4 |
TreeFami | TF326495 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 2.30.29.30, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR017874, CRIC_domain IPR001478, PDZ IPR036034, PDZ_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR001660, SAM IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF10534, CRIC_ras_sig, 1 hit PF00169, PH, 1 hit PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS51290, CRIC, 1 hit PS50106, PDZ, 1 hit PS50003, PH_DOMAIN, 1 hit PS50105, SAM_DOMAIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q969H4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEPVETWTPG KVATWLRGLD DSLQDYPFED WQLPGKNLLQ LCPQSLEALA
60 70 80 90 100
VRSLGHQELI LGGVEQLQAL SSRLQTENLQ SLTEGLLGAT HDFQSIVQGC
110 120 130 140 150
LGDCAKTPID VLCAAVELLH EADALLFWLS RYLFSHLNDF SACQEIRDLL
160 170 180 190 200
EELSQVLHED GPAAEKEGTV LRICSHVAGI CHNILVCCPK ELLEQKAVLE
210 220 230 240 250
QVQLDSPLGL EIHTTSNCQH FVSQVDTQVP TDSRLQIQPG DEVVQINEQV
260 270 280 290 300
VVREERDMVG WPRKNMVREL LREPAGLSLV LKKIPIPETP PQTPPQVLDS
310 320 330 340 350
PHQRSPSLSL APLSPRAPSE DVFAFDLSSN PSPGPSPAWT DSASLGPEPL
360 370 380 390 400
PIPPEPPAIL PAGVAGTPGL PESPDKSPVG RKKSKGLATR LSRRRVSCRE
410 420 430 440 450
LGRPDCDGWL LLRKAPGGFM GPRWRRRWFV LKGHTLYWYR QPQDEKAEGL
460 470 480 490 500
INVSNYSLES GHDQKKKYVF QLTHDVYKPF IFAADTLTDL SMWVRHLITC
510 520 530 540 550
ISKYQSPGRA PPPREEDCYS ETEAEDPDDE AGSHSASPSP AQAGSPLHGD
560 570 580 590 600
TSPAATPTQR SPRTSFGSLT DSSEEALEGM VRGLRQGGVS LLGQPQPLTQ
610 620 630 640 650
EQWRSSFMRR NRDPQLNERV HRVRALQSTL KAKLQELQVL EEVLGDPELT
660 670 680 690 700
GEKFRQWKEQ NRELYSEGLG AWGVAQAEGS SHILTSDSTE QSPHSLPSDP
710 720
EEHSHLCPLT SESSLRPPDL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V160 | G3V160_HUMAN | Connector enhancer of kinase suppre... | CNKSR1 hCG_40691 | 455 | Annotation score: | ||
B4DXN4 | B4DXN4_HUMAN | Connector enhancer of kinase suppre... | CNKSR1 | 312 | Annotation score: | ||
E9PS82 | E9PS82_HUMAN | Connector enhancer of kinase suppre... | CNKSR1 | 229 | Annotation score: | ||
H0YDP5 | H0YDP5_HUMAN | Connector enhancer of kinase suppre... | CNKSR1 | 78 | Annotation score: | ||
E9PIE0 | E9PIE0_HUMAN | Connector enhancer of kinase suppre... | CNKSR1 | 63 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 694 | H → N in AAC80558 (PubMed:9814705).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057790 | 662 | R → W. Corresponds to variant dbSNP:rs17163640Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010886 | 253 – 259 | Missing in isoform 2. 1 Publication | 7 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF100153 mRNA Translation: AAC80558.1 BT006900 mRNA Translation: AAP35546.1 AL355877 Genomic DNA No translation available. AL391650 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07842.1 BC011604 mRNA Translation: AAH11604.1 BC012797 mRNA Translation: AAH12797.1 |
CCDSi | CCDS276.1 [Q969H4-2] CCDS72732.1 [Q969H4-1] |
RefSeqi | NP_001284576.1, NM_001297647.1 [Q969H4-1] NP_001284577.1, NM_001297648.1 NP_006305.2, NM_006314.2 [Q969H4-2] |
Genome annotation databases
Ensembli | ENST00000361530; ENSP00000354609; ENSG00000142675 [Q969H4-2] ENST00000374253; ENSP00000363371; ENSG00000142675 |
GeneIDi | 10256 |
KEGGi | hsa:10256 |
MANE-Selecti | ENST00000361530.11; ENSP00000354609.6; NM_006314.3; NP_006305.2 [Q969H4-2] |
UCSCi | uc001blm.5, human [Q969H4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF100153 mRNA Translation: AAC80558.1 BT006900 mRNA Translation: AAP35546.1 AL355877 Genomic DNA No translation available. AL391650 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07842.1 BC011604 mRNA Translation: AAH11604.1 BC012797 mRNA Translation: AAH12797.1 |
CCDSi | CCDS276.1 [Q969H4-2] CCDS72732.1 [Q969H4-1] |
RefSeqi | NP_001284576.1, NM_001297647.1 [Q969H4-1] NP_001284577.1, NM_001297648.1 NP_006305.2, NM_006314.2 [Q969H4-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WWV | NMR | - | A | 1-78 | [»] | |
SMRi | Q969H4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115550, 25 interactors |
CORUMi | Q969H4 |
IntActi | Q969H4, 16 interactors |
MINTi | Q969H4 |
STRINGi | 9606.ENSP00000363371 |
Chemistry databases
ChEMBLi | CHEMBL4296242 |
PTM databases
iPTMneti | Q969H4 |
PhosphoSitePlusi | Q969H4 |
Genetic variation databases
BioMutai | CNKSR1 |
DMDMi | 50400606 |
Proteomic databases
jPOSTi | Q969H4 |
MassIVEi | Q969H4 |
MaxQBi | Q969H4 |
PaxDbi | Q969H4 |
PeptideAtlasi | Q969H4 |
PRIDEi | Q969H4 |
ProteomicsDBi | 75763 [Q969H4-1] 75764 [Q969H4-2] |
Protocols and materials databases
Antibodypediai | 30569, 153 antibodies from 26 providers |
DNASUi | 10256 |
Genome annotation databases
Ensembli | ENST00000361530; ENSP00000354609; ENSG00000142675 [Q969H4-2] ENST00000374253; ENSP00000363371; ENSG00000142675 |
GeneIDi | 10256 |
KEGGi | hsa:10256 |
MANE-Selecti | ENST00000361530.11; ENSP00000354609.6; NM_006314.3; NP_006305.2 [Q969H4-2] |
UCSCi | uc001blm.5, human [Q969H4-1] |
Organism-specific databases
CTDi | 10256 |
DisGeNETi | 10256 |
GeneCardsi | CNKSR1 |
HGNCi | HGNC:19700, CNKSR1 |
HPAi | ENSG00000142675, Tissue enhanced (skeletal) |
MIMi | 603272, gene |
neXtProti | NX_Q969H4 |
OpenTargetsi | ENSG00000142675 |
PharmGKBi | PA134901167 |
VEuPathDBi | HostDB:ENSG00000142675 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1738, Eukaryota |
GeneTreei | ENSGT00940000159599 |
HOGENOMi | CLU_013414_1_0_1 |
InParanoidi | Q969H4 |
OMAi | WRLNSYD |
PhylomeDBi | Q969H4 |
TreeFami | TF326495 |
Enzyme and pathway databases
PathwayCommonsi | Q969H4 |
Reactomei | R-HSA-5674135, MAP2K and MAPK activation R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants R-HSA-6802948, Signaling by high-kinase activity BRAF mutants R-HSA-6802952, Signaling by BRAF and RAF1 fusions R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF R-HSA-9649948, Signaling downstream of RAS mutants R-HSA-9656223, Signaling by RAF1 mutants |
SignaLinki | Q969H4 |
SIGNORi | Q969H4 |
Miscellaneous databases
BioGRID-ORCSi | 10256, 8 hits in 1032 CRISPR screens |
ChiTaRSi | CNKSR1, human |
EvolutionaryTracei | Q969H4 |
GeneWikii | CNKSR1 |
GenomeRNAii | 10256 |
Pharosi | Q969H4, Tbio |
PROi | PR:Q969H4 |
RNActi | Q969H4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000142675, Expressed in muscle of leg and 220 other tissues |
ExpressionAtlasi | Q969H4, baseline and differential |
Genevisiblei | Q969H4, HS |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 2.30.29.30, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR017874, CRIC_domain IPR001478, PDZ IPR036034, PDZ_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR001660, SAM IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF10534, CRIC_ras_sig, 1 hit PF00169, PH, 1 hit PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS51290, CRIC, 1 hit PS50106, PDZ, 1 hit PS50003, PH_DOMAIN, 1 hit PS50105, SAM_DOMAIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CNKR1_HUMAN | |
Accessioni | Q969H4Primary (citable) accession number: Q969H4 Secondary accession number(s): B1AMW9, O95381 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families