UniProtKB - Q969G6 (RIFK_HUMAN)
Riboflavin kinase
RFK
Functioni
Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
1 PublicationCatalytic activityi
Cofactori
: FMN biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).1 Publication This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 15 | ATP; via amide nitrogenCombined sources2 Publications | 1 | |
Binding sitei | 21 | ATP; via amide nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 27 | MagnesiumCombined sources1 Publication | 1 | |
Binding sitei | 27 | ATP; via amide nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 29 | MagnesiumCombined sources1 Publication | 1 | |
Binding sitei | 29 | ATPCombined sources2 Publications | 1 | |
Active sitei | 79 | NucleophileBy similarity | 1 | |
Binding sitei | 82 | ATP; via amide nitrogen and carbonyl oxygenCombined sources2 Publications | 1 | |
Binding sitei | 84 | ATP; via carbonyl oxygenCombined sources2 Publications | 1 | |
Binding sitei | 91 | ATPCombined sources2 Publications | 1 | |
Binding sitei | 104 | FMNCombined sources2 Publications | 1 | |
Binding sitei | 107 | FMN; via amide nitrogen and carbonyl oxygenCombined sources2 Publications | 1 | |
Binding sitei | 109 | FMN; via amide nitrogenCombined sources2 Publications | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- riboflavin kinase activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: MGI
- FMN biosynthetic process Source: GO_Central
- positive regulation of NAD(P)H oxidase activity Source: MGI
- reactive oxygen species metabolic process Source: Ensembl
- riboflavin biosynthetic process Source: UniProtKB
- riboflavin metabolic process Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 2.7.1.26, 2681 |
PathwayCommonsi | Q969G6 |
Reactomei | R-HSA-196843, Vitamin B2 (riboflavin) metabolism |
SignaLinki | Q969G6 |
UniPathwayi | UPA00276;UER00406 |
Names & Taxonomyi
Protein namesi | Recommended name: Riboflavin kinase1 Publication (EC:2.7.1.261 Publication)Alternative name(s): ATP:riboflavin 5'-phosphotransferase Flavokinase |
Gene namesi | Name:RFK |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30324, RFK |
MIMi | 613010, gene |
neXtProti | NX_Q969G6 |
VEuPathDBi | HostDB:ENSG00000135002 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 79 | E → Q: Loss of riboflavin kinase activity. No effect on TNFRSF1A- and CYBA-binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55312 |
OpenTargetsi | ENSG00000135002 |
PharmGKBi | PA134916697 |
Miscellaneous databases
Pharosi | Q969G6, Tbio |
Chemistry databases
DrugBanki | DB03247, Flavin mononucleotide DB00140, Riboflavin |
DrugCentrali | Q969G6 |
Genetic variation databases
BioMutai | RFK |
DMDMi | 209572667 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194148 | 1 – 155 | Riboflavin kinaseAdd BLAST | 155 |
Proteomic databases
EPDi | Q969G6 |
jPOSTi | Q969G6 |
MassIVEi | Q969G6 |
MaxQBi | Q969G6 |
PaxDbi | Q969G6 |
PeptideAtlasi | Q969G6 |
PRIDEi | Q969G6 |
ProteomicsDBi | 75757 |
PTM databases
GlyGeni | Q969G6, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q969G6 |
PhosphoSitePlusi | Q969G6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000135002, Expressed in colonic mucosa and 255 other tissues |
ExpressionAtlasi | Q969G6, baseline and differential |
Genevisiblei | Q969G6, HS |
Organism-specific databases
HPAi | ENSG00000135002, Low tissue specificity |
Interactioni
Subunit structurei
Monomer. Directly interacts with TNFRSF1A death domain. TNFRSF1A-binding may be supported by TRADD. In the absence of TNFRSF1A, interacts with TRADD. Independently of TNFRSF1A, interacts with the NADPH oxidase subunit CYBA.
2 PublicationsBinary interactionsi
Q969G6
With | #Exp. | IntAct |
---|---|---|
TNFRSF1A [P19438] | 4 | EBI-716872,EBI-299451 |
TNFRSF1A - isoform 1 [P19438-1] | 2 | EBI-716872,EBI-15795644 |
Protein-protein interaction databases
BioGRIDi | 120594, 17 interactors |
DIPi | DIP-60454N |
IntActi | Q969G6, 5 interactors |
STRINGi | 9606.ENSP00000365926 |
Miscellaneous databases
RNActi | Q969G6, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q969G6 |
SMRi | Q969G6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q969G6 |
Family & Domainsi
Phylogenomic databases
eggNOGi | KOG3110, Eukaryota |
GeneTreei | ENSGT00390000015537 |
HOGENOMi | CLU_048437_3_3_1 |
InParanoidi | Q969G6 |
OMAi | EVYKMVL |
OrthoDBi | 1534271at2759 |
PhylomeDBi | Q969G6 |
TreeFami | TF313786 |
Family and domain databases
Gene3Di | 2.40.30.30, 1 hit |
InterProi | View protein in InterPro IPR023468, Riboflavin_kinase IPR015865, Riboflavin_kinase_bac/euk IPR023465, Riboflavin_kinase_dom_sf |
PANTHERi | PTHR22749, PTHR22749, 1 hit |
Pfami | View protein in Pfam PF01687, Flavokinase, 1 hit |
SMARTi | View protein in SMART SM00904, Flavokinase, 1 hit |
SUPFAMi | SSF82114, SSF82114, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW
60 70 80 90 100
ASVGSGDVHK MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV
110 120 130 140 150
GYLRPEKNFD SLESLISAIQ GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK
IMNGH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH7C4G0 | H7C4G0_HUMAN | Riboflavin kinase | RFK | 120 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | N → S in BAA92033 (PubMed:14702039).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002011 mRNA Translation: BAA92033.1 Different initiation. AL391868 Genomic DNA No translation available. BC007069 mRNA Translation: AAH07069.1 Different initiation. |
CCDSi | CCDS35044.2 |
RefSeqi | NP_060809.3, NM_018339.5 |
Genome annotation databases
Ensembli | ENST00000376736.6; ENSP00000365926.1; ENSG00000135002.12 |
GeneIDi | 55312 |
KEGGi | hsa:55312 |
MANE-Selecti | ENST00000376736.6; ENSP00000365926.1; NM_018339.6; NP_060809.3 |
UCSCi | uc004akd.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002011 mRNA Translation: BAA92033.1 Different initiation. AL391868 Genomic DNA No translation available. BC007069 mRNA Translation: AAH07069.1 Different initiation. |
CCDSi | CCDS35044.2 |
RefSeqi | NP_060809.3, NM_018339.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NB0 | X-ray | 1.70 | A | 2-148 | [»] | |
1NB9 | X-ray | 1.70 | A | 2-148 | [»] | |
1P4M | X-ray | 1.80 | A | 2-148 | [»] | |
1Q9S | X-ray | 2.42 | A | 1-148 | [»] | |
AlphaFoldDBi | Q969G6 | |||||
SMRi | Q969G6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120594, 17 interactors |
DIPi | DIP-60454N |
IntActi | Q969G6, 5 interactors |
STRINGi | 9606.ENSP00000365926 |
Chemistry databases
DrugBanki | DB03247, Flavin mononucleotide DB00140, Riboflavin |
DrugCentrali | Q969G6 |
PTM databases
GlyGeni | Q969G6, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q969G6 |
PhosphoSitePlusi | Q969G6 |
Genetic variation databases
BioMutai | RFK |
DMDMi | 209572667 |
Proteomic databases
EPDi | Q969G6 |
jPOSTi | Q969G6 |
MassIVEi | Q969G6 |
MaxQBi | Q969G6 |
PaxDbi | Q969G6 |
PeptideAtlasi | Q969G6 |
PRIDEi | Q969G6 |
ProteomicsDBi | 75757 |
Protocols and materials databases
Antibodypediai | 27259, 155 antibodies from 24 providers |
DNASUi | 55312 |
Genome annotation databases
Ensembli | ENST00000376736.6; ENSP00000365926.1; ENSG00000135002.12 |
GeneIDi | 55312 |
KEGGi | hsa:55312 |
MANE-Selecti | ENST00000376736.6; ENSP00000365926.1; NM_018339.6; NP_060809.3 |
UCSCi | uc004akd.3, human |
Organism-specific databases
CTDi | 55312 |
DisGeNETi | 55312 |
GeneCardsi | RFK |
HGNCi | HGNC:30324, RFK |
HPAi | ENSG00000135002, Low tissue specificity |
MIMi | 613010, gene |
neXtProti | NX_Q969G6 |
OpenTargetsi | ENSG00000135002 |
PharmGKBi | PA134916697 |
VEuPathDBi | HostDB:ENSG00000135002 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3110, Eukaryota |
GeneTreei | ENSGT00390000015537 |
HOGENOMi | CLU_048437_3_3_1 |
InParanoidi | Q969G6 |
OMAi | EVYKMVL |
OrthoDBi | 1534271at2759 |
PhylomeDBi | Q969G6 |
TreeFami | TF313786 |
Enzyme and pathway databases
UniPathwayi | UPA00276;UER00406 |
BRENDAi | 2.7.1.26, 2681 |
PathwayCommonsi | Q969G6 |
Reactomei | R-HSA-196843, Vitamin B2 (riboflavin) metabolism |
SignaLinki | Q969G6 |
Miscellaneous databases
BioGRID-ORCSi | 55312, 257 hits in 1045 CRISPR screens |
ChiTaRSi | RFK, human |
EvolutionaryTracei | Q969G6 |
GenomeRNAii | 55312 |
Pharosi | Q969G6, Tbio |
PROi | PR:Q969G6 |
RNActi | Q969G6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000135002, Expressed in colonic mucosa and 255 other tissues |
ExpressionAtlasi | Q969G6, baseline and differential |
Genevisiblei | Q969G6, HS |
Family and domain databases
Gene3Di | 2.40.30.30, 1 hit |
InterProi | View protein in InterPro IPR023468, Riboflavin_kinase IPR015865, Riboflavin_kinase_bac/euk IPR023465, Riboflavin_kinase_dom_sf |
PANTHERi | PTHR22749, PTHR22749, 1 hit |
Pfami | View protein in Pfam PF01687, Flavokinase, 1 hit |
SMARTi | View protein in SMART SM00904, Flavokinase, 1 hit |
SUPFAMi | SSF82114, SSF82114, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RIFK_HUMAN | |
Accessioni | Q969G6Primary (citable) accession number: Q969G6 Secondary accession number(s): Q5JSG9, Q9NUT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | October 14, 2008 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references