RFK

Functionⁱ

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.1 Publication

Catalytic activityⁱ

ATP + riboflavin = ADP + FMN.

Cofactorⁱ

Zn²⁺, Mg²⁺Note: Zinc or magnesium.

Pathwayⁱ: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).
Proteins known to be involved in this subpathway in this organism are:

Riboflavin kinase (RFK)

This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	15	ATP; via amide nitrogen	1
Binding siteⁱ	21	ATP; via amide nitrogen	1
Metal bindingⁱ	27	Magnesium	1
Binding siteⁱ	27	ATP; via amide nitrogen	1
Binding siteⁱ	29	ATP	1
Active siteⁱ	79	NucleophileBy similarity	1
Binding siteⁱ	82	ATP; via amide nitrogen and carbonyl oxygen	1
Binding siteⁱ	84	ATP; via carbonyl oxygen	1
Binding siteⁱ	91	ATP	1
Binding siteⁱ	104	FMN	1
Binding siteⁱ	107	FMN; via amide nitrogen and carbonyl oxygen	1
Binding siteⁱ	109	FMN; via amide nitrogen	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW
riboflavin kinase activity Source: Reactome

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 19641494
FMN biosynthetic process Source: UniProtKB-UniPathway
positive regulation of NAD(P)H oxidase activity
Source: MGI
Inferred from mutant phenotypeⁱ
- 19641494
reactive oxygen species metabolic process Source: Ensembl
riboflavin biosynthetic process
Source: UniProtKB
Non-traceable author statementⁱ
- 12623014
riboflavin metabolic process Source: Reactome

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Ligand	ATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS05938-MONOMER
BRENDAⁱ	2.7.1.26 2681
Reactomeⁱ	R-HSA-196843 Vitamin B2 (riboflavin) metabolism
UniPathwayⁱ	UPA00276; UER00406

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Riboflavin kinase (EC:2.7.1.26) Alternative name(s): ATP:riboflavin 5'-phosphotransferase Flavokinase
Gene namesⁱ	Name:RFK
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 9

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000135002.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:30324 RFK
MIMⁱ	613010 gene
neXtProtⁱ	NX_Q969G6

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	79	E → Q: Loss of kinase activity. No effect on TNFRSF1A- and CYBA-binding. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	55312
Open Targets More...OpenTargetsⁱ	ENSG00000135002
PharmGKBⁱ	PA134916697

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB00140 Riboflavin DB03247 Riboflavin Monophosphate

DrugBankⁱ

DB00140 Riboflavin
DB03247 Riboflavin Monophosphate

Polymorphism and mutation databases

BioMutaⁱ	RFK
DMDMⁱ	209572667

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000194148	1 – 155	Riboflavin kinaseAdd BLAST		155

Proteomic databases

EPDⁱ	Q969G6
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q969G6
PaxDbⁱ	Q969G6
PeptideAtlas More...PeptideAtlasⁱ	Q969G6
PRIDEⁱ	Q969G6
ProteomicsDBⁱ	75757

PTM databases

iPTMnetⁱ	Q969G6
PhosphoSitePlusⁱ	Q969G6

Expressionⁱ

Tissue specificityⁱ

Detected in brain, placenta and urinary bladder.

Gene expression databases

Bgeeⁱ	ENSG00000135002
CleanExⁱ	HS_RFK
ExpressionAtlasⁱ	Q969G6 baseline and differential
Genevisibleⁱ	Q969G6 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB033887 HPA023259 HPA057163

HPAⁱ

CAB033887
HPA023259
HPA057163

Interactionⁱ

Subunit structureⁱ

Monomer. Directly interacts with TNFRSF1A death domain. TNFRSF1A-binding may be supported by TRADD. In the absence of TNFRSF1A, interacts with TRADD. Independently of TNFRSF1A, interacts with the NADPH oxidase subunit CYBA.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
TNFRSF1A	P19438	4	EBI-716872,EBI-299451
TNFRSF1A	P19438-1	2	EBI-716872,EBI-15795644

Show more details

Protein-protein interaction databases

BioGridⁱ	120594, 8 interactors
Database of interacting proteins More...DIPⁱ	DIP-60454N
IntActⁱ	Q969G6, 5 interactors
STRINGⁱ	9606.ENSP00000365926

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	5 – 11	Combined sources	7
Beta strandⁱ	16 – 18	Combined sources	3
Helixⁱ	20 – 23	Combined sources	4
Helixⁱ	32 – 36	Combined sources	5
Beta strandⁱ	44 – 53	Combined sources	10
Beta strandⁱ	59 – 67	Combined sources	9
Beta strandⁱ	69 – 74	Combined sources	6
Beta strandⁱ	76 – 84	Combined sources	9
Beta strandⁱ	93 – 104	Combined sources	12
Helixⁱ	112 – 129	Combined sources	18
Helixⁱ	133 – 136	Combined sources	4
Helixⁱ	137 – 140	Combined sources	4
Helixⁱ	142 – 146	Combined sources	5

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1NB0	X-ray	1.70	A	2-148	[»]
	1NB9	X-ray	1.70	A	2-148	[»]
	1P4M	X-ray	1.80	A	2-148	[»]
	1Q9S	X-ray	2.42	A	1-148	[»]
ProteinModelPortalⁱ	Q969G6
SMRⁱ	Q969G6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q969G6

EvolutionaryTraceⁱ

Q969G6

Family & Domainsⁱ

Phylogenomic databases

eggNOGⁱ	KOG3110 Eukaryota COG0196 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000015537
HOGENOMⁱ	HOG000260803
HOVERGENⁱ	HBG049989
InParanoidⁱ	Q969G6
KEGG Orthology (KO) More...KOⁱ	K00861
OMAⁱ	GKDLGCP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0Z4E
PhylomeDBⁱ	Q969G6
TreeFamⁱ	TF313786

Family and domain databases

Gene3Dⁱ	2.40.30.30, 1 hit
InterProⁱ	View protein in InterPro IPR023468 Riboflavin_kinase IPR015865 Riboflavin_kinase_bac/euk IPR023465 Riboflavin_kinase_dom_sf
PANTHERⁱ	PTHR22749 PTHR22749, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01687 Flavokinase, 1 hit
SMARTⁱ	View protein in SMART SM00904 Flavokinase, 1 hit
SUPFAMⁱ	SSF82114 SSF82114, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q969G6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW 
        60         70         80         90        100
ASVGSGDVHK MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV 
       110        120        130        140        150
GYLRPEKNFD SLESLISAIQ GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK 

IMNGH

Length:155

Mass (Da):17,623

Last modified:October 14, 2008 - v2

Checksum:ⁱ3E038E487E164EBA

GO

Sequence cautionⁱ

The sequence AAH07069 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence BAA92033 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	96	N → S in BAA92033 (PubMed:14702039).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK002011 mRNA Translation: BAA92033.1 Different initiation. AL391868 Genomic DNA No translation available. BC007069 mRNA Translation: AAH07069.1 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS35044.2
NCBI Reference Sequences More...RefSeqⁱ	NP_060809.3, NM_018339.5
UniGeneⁱ	Hs.37558

Genome annotation databases

Ensemblⁱ	ENST00000376736; ENSP00000365926; ENSG00000135002
GeneIDⁱ	55312
KEGGⁱ	hsa:55312
UCSC genome browser More...UCSCⁱ	uc004akd.3 human

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q969G6	RFK isoform 1		PANTR		155
Riboflavin kinase, isoform CRA_a		HUMAN		162
Riboflavin kinase		PANPA		162
UPI0008F50F3B		GORGO		95
Uncharacterized protein		SAIBB		162
+89

Protein	Similar proteins		Species	Score	Length
Q969G6	RFK isoform 1		PANTR		155
Riboflavin kinase, isoform CRA_a		HUMAN		162
Riboflavin kinase		PANPA		162
UPI0008F50F3B		GORGO		95
UPI00006D5F63		MACMU		162
+135

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK002011 mRNA Translation: BAA92033.1 Different initiation. AL391868 Genomic DNA No translation available. BC007069 mRNA Translation: AAH07069.1 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS35044.2
NCBI Reference Sequences More...RefSeqⁱ	NP_060809.3, NM_018339.5
UniGeneⁱ	Hs.37558

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1NB0	X-ray	1.70	A	2-148	[»]
	1NB9	X-ray	1.70	A	2-148	[»]
	1P4M	X-ray	1.80	A	2-148	[»]
	1Q9S	X-ray	2.42	A	1-148	[»]
ProteinModelPortalⁱ	Q969G6
SMRⁱ	Q969G6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	120594, 8 interactors
Database of interacting proteins More...DIPⁱ	DIP-60454N
IntActⁱ	Q969G6, 5 interactors
STRINGⁱ	9606.ENSP00000365926

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB00140 Riboflavin DB03247 Riboflavin Monophosphate

DrugBankⁱ

DB00140 Riboflavin
DB03247 Riboflavin Monophosphate

PTM databases

iPTMnetⁱ	Q969G6
PhosphoSitePlusⁱ	Q969G6

Polymorphism and mutation databases

BioMutaⁱ	RFK
DMDMⁱ	209572667

Proteomic databases

EPDⁱ	Q969G6
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q969G6
PaxDbⁱ	Q969G6
PeptideAtlas More...PeptideAtlasⁱ	Q969G6
PRIDEⁱ	Q969G6
ProteomicsDBⁱ	75757

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	55312
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000376736; ENSP00000365926; ENSG00000135002
GeneIDⁱ	55312
KEGGⁱ	hsa:55312
UCSC genome browser More...UCSCⁱ	uc004akd.3 human

Organism-specific databases

CTDⁱ	55312
DisGeNET More...DisGeNETⁱ	55312
EuPathDBⁱ	HostDB:ENSG00000135002.11
GeneCardsⁱ	RFK
H-InvDBⁱ	HIX0169330
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:30324 RFK
Human Protein Atlas More...HPAⁱ	CAB033887 HPA023259 HPA057163
MIMⁱ	613010 gene
neXtProtⁱ	NX_Q969G6
Open Targets More...OpenTargetsⁱ	ENSG00000135002
PharmGKBⁱ	PA134916697
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3110 Eukaryota COG0196 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000015537
HOGENOMⁱ	HOG000260803
HOVERGENⁱ	HBG049989
InParanoidⁱ	Q969G6
KEGG Orthology (KO) More...KOⁱ	K00861
OMAⁱ	GKDLGCP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0Z4E
PhylomeDBⁱ	Q969G6
TreeFamⁱ	TF313786

Enzyme and pathway databases

UniPathwayⁱ	UPA00276; UER00406
BioCycⁱ	MetaCyc:HS05938-MONOMER
BRENDAⁱ	2.7.1.26 2681
Reactomeⁱ	R-HSA-196843 Vitamin B2 (riboflavin) metabolism

Miscellaneous databases

ChiTaRSⁱ	RFK human
EvolutionaryTraceⁱ	Q969G6
GenomeRNAiⁱ	55312
Protein Ontology More...PROⁱ	PR:Q969G6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000135002
CleanExⁱ	HS_RFK
ExpressionAtlasⁱ	Q969G6 baseline and differential
Genevisibleⁱ	Q969G6 HS

Family and domain databases

Gene3Dⁱ	2.40.30.30, 1 hit
InterProⁱ	View protein in InterPro IPR023468 Riboflavin_kinase IPR015865 Riboflavin_kinase_bac/euk IPR023465 Riboflavin_kinase_dom_sf
PANTHERⁱ	PTHR22749 PTHR22749, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01687 Flavokinase, 1 hit
SMARTⁱ	View protein in SMART SM00904 Flavokinase, 1 hit
SUPFAMⁱ	SSF82114 SSF82114, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RIFK_HUMAN
Accessionⁱ	Q969G6Primary (citable) accession number: Q969G6 Secondary accession number(s): Q5JSG9, Q9NUT7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
	Last sequence update:	October 14, 2008
	Last modified:	June 20, 2018
	This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

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Help

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Annotation systems

Supporting data

UniProtKB - Q969G6 (RIFK_HUMAN)

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Riboflavin kinase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: FMN biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: FMN biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ