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Protein

Pyruvate dehydrogenase [NADP(+)]

Gene

PFOR

Organism
Cryptosporidium parvum
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May have an important role in respiratory metabolism. Cryptosporidium have a relic mitochondrion with no function in energy metabolism so it is not known if PFOR has a function.1 Publication

Miscellaneous

Arose from gene fusion of pyruvate:ferredoxin oxidoreductase and cytochrome-P450 reductase. Gene fusion has only been found in Euglena and Cryptosporidium.1 Publication

Catalytic activityi

Pyruvate + CoA + NADP+ = acetyl-CoA + CO2 + NADPH.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi719Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi722Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi725Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi729Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi776Iron-sulfur 2 (4Fe-4S)Sequence analysis1
Metal bindingi779Iron-sulfur 2 (4Fe-4S)Sequence analysis1
Metal bindingi782Iron-sulfur 2 (4Fe-4S)Sequence analysis1
Metal bindingi786Iron-sulfur 2 (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1542 – 1553FADBy similarityAdd BLAST12
Nucleotide bindingi1685 – 1695FADBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
Ligand4Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NADP, Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase [NADP(+)] (EC:1.2.1.51)
Alternative name(s):
CpPNO
Pyruvate:NADP(+) oxidoreductase
Gene namesi
Name:PFOR
OrganismiCryptosporidium parvumImported
Taxonomic identifieri5807 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaCryptosporidiidaeCryptosporidium

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2364026

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002155591 – 1934Pyruvate dehydrogenase [NADP(+)]Add BLAST1934

Proteomic databases

PRIDEiQ968X7

Expressioni

Developmental stagei

Both sporozoites and intracellular stages of life cycle.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ968X7
SMRiQ968X7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini710 – 7394Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini767 – 7964Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30
Domaini1288 – 1438Flavodoxin-likePROSITE-ProRule annotationAdd BLAST151
Domaini1501 – 1759FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST259

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
KOG1159 Eukaryota
COG0369 LUCA
COG0674 LUCA
COG1013 LUCA
COG1014 LUCA

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit
3.40.50.920, 1 hit
3.40.920.10, 1 hit
4.10.780.10, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR003097 CysJ-like_FAD-binding
IPR017927 FAD-bd_FR_type
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR033412 PFOR_II
IPR037112 Pyrv-flavodox_OxR_EKR_sf
IPR019456 Pyrv-flavodox_OxRtase_EKR
IPR019752 Pyrv/ketoisovalerate_OxRed_cat
IPR002880 Pyrv_Fd/Flavodoxin_OxRdtase_N
IPR011895 Pyrv_flavodox_OxRed
IPR002869 Pyrv_flavodox_OxRed_cen
IPR017938 Riboflavin_synthase-like_b-brl
IPR029061 THDP-binding
IPR011766 TPP_enzyme-bd_C
IPR009014 Transketo_C/PFOR_II
PfamiView protein in Pfam
PF10371 EKR, 1 hit
PF00667 FAD_binding_1, 1 hit
PF12838 Fer4_7, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PF17147 PFOR_II, 1 hit
PF01558 POR, 1 hit
PF01855 POR_N, 1 hit
PF02775 TPP_enzyme_C, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SMARTiView protein in SMART
SM00890 EKR, 1 hit
SUPFAMiSSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF52518 SSF52518, 3 hits
SSF52922 SSF52922, 1 hit
SSF53323 SSF53323, 2 hits
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR02176 pyruv_ox_red, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q968X7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGEKEIVDGC VAACHIAYAC SEVAFTYPIT PSSTISEVAD SWMSRGRRNI
60 70 80 90 100
FDQVVSVVEM QSEMGSAGAL HGSLSVGCST TTFTASQGLL LMIPNMYKIA
110 120 130 140 150
GELWPCVFHV TARAIATSSL SIFGDHNDIM AARQTGWAFL GAMTVQEVMD
160 170 180 190 200
LALVSHVSTF ECSVPFVNFF DGFRTSHELQ KIDMISYETI KKIFPYEKLK
210 220 230 240 250
EFRERALNPT HPTLRGTATS SDVYFQLAEA RNKYYESTPD IVQSVMDRLA
260 270 280 290 300
KLIGRSYHLF DYYGHPDAEF LIVVMGSGGL TIEEMIDYLM EKSNEKVGMI
310 320 330 340 350
KVRLFRPWSI DAFVKKIPKT TKRITVLERC KESGSLGEPL CLDVSTSIMR
360 370 380 390 400
SELSSNNILV LGGRYGLASK EFTPGMALAV WENMISENPI NNFSVGIDDD
410 420 430 440 450
VTFKSLFVRQ PRLDLLTSET KQCLFWGLGS DGTVSANKNA IKIIGESTDL
460 470 480 490 500
QVQGYFAYDA KKAGGATMSH LRFGPKPIKS AYLLQRCDYV AVHHPSYVHK
510 520 530 540 550
FDVLENIKQG GCFVLNCPWS TLEELNHELP SKIKHQIASR DVKFYVIDAQ
560 570 580 590 600
RIAQESNLGR RINNILMVVF FSLTNIIPLD LAIKLVKEAI KKTYGKKGDA
610 620 630 640 650
VVNSNWKAVD LTLESLIQIS YDKSQWISKD KCGEKSLPAT AVETGNKDQE
660 670 680 690 700
ITKSTVLKQK PEHDVNQFVK DILGPVNALK GDELPVSMFE PTGTVPLGTT
710 720 730 740 750
AYEKRGIAMS IPIVDMNKCT QCNYCSIVCP HAAIRPFLLD EAEFKNAPET
760 770 780 790 800
MHIPKAKGGQ EFSSYYYRIQ VTPLDCTGCE LCVHACPDDA LHMEGLQKME
810 820 830 840 850
AVEKTHWDYL IGLPNKAEKF DRTTVKGSQF QQPLLEFSAA CEGCGETPYV
860 870 880 890 900
KLLTQLFGER MVIANATGCS SIWGASYPSV PYTKNQKGYG PAWGNSLFED
910 920 930 940 950
NAEYGLGMVV GYRQRRDRFR ELVSNEILKD ITEEEEFLKD DNASVQGRNE
960 970 980 990 1000
IITKYDHLKD YLRSWLKNIR NGEACQSLFE EISKLLEDNL INSNNFAQVL
1010 1020 1030 1040 1050
KKDRIELLEK LYDSRDLIPK ISHWIVGGDG WAYDIGYAGL DHVLSFGEDV
1060 1070 1080 1090 1100
NIIILDTEVY SNTGGQASKS TPFGAIAKFA QSGNLRQKKD IGSIAMEYGS
1110 1120 1130 1140 1150
VYVASVALGA NYSQTIKSLL EAEKYPGTSL IVAYSTCIEH GYTKYNLQQE
1160 1170 1180 1190 1200
SVKLAVESGY WPLYRYNPEL VRTEVVDNLT TIVSSGFTLD SKKVKVDIEN
1210 1220 1230 1240 1250
FLKRENRFLQ LIRSNPELAS MAKDKLKAHS DKRFQKMKDM SENVTVTALK
1260 1270 1280 1290 1300
DQIKKLKDQL ISIQNASKTG ELAASGLINA DLFIEQEMHV LYGTETGNSE
1310 1320 1330 1340 1350
EVAQYIQSQL VSRGYSSSSL NLDDLDIDEF LNPDKFSTVI IVTSTSGQGE
1360 1370 1380 1390 1400
FPGSSGILYE ALLKKHLENQ DDKFCSFMRF GIFGLGDSNY VFFNEAAKKW
1410 1420 1430 1440 1450
DKLLLDCGAV RIGAVGMGDD QSEEKYETEL IEWLPDYLQL INAPEPKHDE
1460 1470 1480 1490 1500
KSEIPKATTF KVTILDSCRN DILNESTGTL CEKLDENNNI GNSHYKPIIP
1510 1520 1530 1540 1550
PNSVLLPVIE NKRITNQDYD KDVRHIVFKL IGDGGDTPSL SYCLGDSLAL
1560 1570 1580 1590 1600
YGQNPVNEAI KAIEMFGYNP YSLLRLSINE ENEANNTNKV NQRYSSLFGY
1610 1620 1630 1640 1650
DITVLQLFVE CLDLWGKPNR KFFQEFYRYC SNPEEKIQAK KWAQNEGKKL
1660 1670 1680 1690 1700
IEEFSSKTGT YLDVFKMFES ARPTLAQLLD IVPFIKSRSY SIASCNKFVN
1710 1720 1730 1740 1750
GEKIELCVGI VDWKLESGEI RYGQCTGFLN RLPILDSESK IDSIPRLPSN
1760 1770 1780 1790 1800
IKASAFNLPF DYRSPVIMAC MGTGIAPFRA FVQNKKYIRD VLKEEIGPVI
1810 1820 1830 1840 1850
LYFGCRYYDN DYLYREELEN YVKEGVITSL NIAFSRDPKG YKTSNCENIR
1860 1870 1880 1890 1900
YAQKMYVQHL MLENSQEIYE NMIEKCGYFY LCGTKQVPID IRKAIIQIII
1910 1920 1930
KHSSTTEQVT SEEDANSILN SIQIMGRYNV EAWS
Length:1,934
Mass (Da):217,557
Last modified:December 1, 2001 - v1
Checksum:iBCDB56F4B2BA3D60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208233 Genomic DNA Translation: AAK48421.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208233 Genomic DNA Translation: AAK48421.1

3D structure databases

ProteinModelPortaliQ968X7
SMRiQ968X7
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL2364026

Proteomic databases

PRIDEiQ968X7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
KOG1159 Eukaryota
COG0369 LUCA
COG0674 LUCA
COG1013 LUCA
COG1014 LUCA

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit
3.40.50.920, 1 hit
3.40.920.10, 1 hit
4.10.780.10, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR003097 CysJ-like_FAD-binding
IPR017927 FAD-bd_FR_type
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR033412 PFOR_II
IPR037112 Pyrv-flavodox_OxR_EKR_sf
IPR019456 Pyrv-flavodox_OxRtase_EKR
IPR019752 Pyrv/ketoisovalerate_OxRed_cat
IPR002880 Pyrv_Fd/Flavodoxin_OxRdtase_N
IPR011895 Pyrv_flavodox_OxRed
IPR002869 Pyrv_flavodox_OxRed_cen
IPR017938 Riboflavin_synthase-like_b-brl
IPR029061 THDP-binding
IPR011766 TPP_enzyme-bd_C
IPR009014 Transketo_C/PFOR_II
PfamiView protein in Pfam
PF10371 EKR, 1 hit
PF00667 FAD_binding_1, 1 hit
PF12838 Fer4_7, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PF17147 PFOR_II, 1 hit
PF01558 POR, 1 hit
PF01855 POR_N, 1 hit
PF02775 TPP_enzyme_C, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SMARTiView protein in SMART
SM00890 EKR, 1 hit
SUPFAMiSSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF52518 SSF52518, 3 hits
SSF52922 SSF52922, 1 hit
SSF53323 SSF53323, 2 hits
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR02176 pyruv_ox_red, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPNO_CRYPV
AccessioniPrimary (citable) accession number: Q968X7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: December 1, 2001
Last modified: October 10, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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