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Protein

Structural polyprotein

Gene
N/A
Organism
Drosophila x virus (isolate Chung/1996) (DXV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Capsid protein VP2 self assembles to form an icosahedral capsid with a T=13 symmetry, about 70 nm in diameter, and consisting of 260 VP2 trimers. The capsid encapsulates the genomic dsRNA. VP2 is also involved in attachment and entry into the host cell (By similarity).By similarity
The precursor of VP2 plays an important role in capsid assembly. First, pre-VP2 and VP2 oligomers assemble to form a procapsid. Then, the pre-VP2 intermediates may be processed into VP2 proteins by proteolytic cleavage mediated by VP4 to obtain the mature virion. The final capsid is composed of pentamers and hexamers but VP2 has a natural tendency to assemble into all-pentameric structures. Therefore pre-VP2 may be required to allow formation of the hexameric structures (By similarity).By similarity
Protease VP4 is a serine protease that cleaves the polyprotein into its final products. Pre-VP2 is first partially cleaved, and may be completely processed by VP4 upon capsid maturation.PROSITE-ProRule annotation
Capsid protein VP3 plays a key role in virion assembly by providing a scaffold for the capsid made of VP2. May self-assemble to form a T=4-like icosahedral inner-capsid composed of at least 180 trimers. Plays a role in genomic RNA packaging by recruiting VP1 into the capsid and interacting with the dsRNA genome segments to form a ribonucleoprotein complex. Additionally, the interaction of the VP3 C-terminal tail with VP1 removes the inherent structural blockade of the polymerase active site. Thus, VP3 can also function as a transcriptional activator (By similarity).By similarity
Structural peptide 1 is a small peptide derived from pre-VP2 C-terminus. It destabilizes and perforates cell membranes, suggesting a role during entry (By similarity).By similarity
Structural peptide 2 is a small peptide derived from pre-VP2 C-terminus. It is not essential for the virus viability, but viral growth is affected when missing (By similarity).By similarity
Structural peptide 3 is a small peptide derived from pre-VP2 C-terminus. It is not essential for the virus viability, but viral growth is affected when missing (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23Divalent metal cation; shared with trimeric partnersBy similarity1
Active sitei627NucleophilePROSITE-ProRule annotation1
Active sitei670PROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandMetal-binding

Protein family/group databases

MEROPSiS50.003

Names & Taxonomyi

Protein namesi
Recommended name:
Structural polyprotein
Short name:
PP
Cleaved into the following 7 chains:
Precursor of VP2
Short name:
pre-VP2
Structural peptide 1
Short name:
p1
Structural peptide 2
Short name:
p2
Structural peptide 3
Short name:
p3
Alternative name(s):
Non-structural protein VP4
Short name:
NS
OrganismiDrosophila x virus (isolate Chung/1996) (DXV)
Taxonomic identifieri654931 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesBirnaviridaeEntomobirnavirus
Virus hostiDrosophila melanogaster (Fruit fly) [TaxID: 7227]
Proteomesi
  • UP000000515 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003783761 – 1032Structural polyproteinAdd BLAST1032
ChainiPRO_00003783771 – 500Precursor of VP2Add BLAST500
ChainiPRO_00003783781 – 431Capsid protein VP2Add BLAST431
PeptideiPRO_0000378379431 – 474Structural peptide 1By similarityAdd BLAST44
PeptideiPRO_0000378380475 – 483Structural peptide 2By similarity9
PeptideiPRO_0000378381484 – 500Structural peptide 3By similarityAdd BLAST17
ChainiPRO_0000378382501 – 723Protease VP4Add BLAST223
ChainiPRO_0000378383724 – 1032Capsid protein VP3Add BLAST309

Post-translational modificationi

Specific enzymatic cleavages yield mature proteins. The capsid assembly seems to be regulated by polyprotein processing. The protease VP4 cleaves itself off the polyprotein, thus releasing pre-VP2 and VP3 within the infected cell. During capsid assembly, the C-terminus of pre-VP2 is further processed by VP4, giving rise to VP2, the external capsid protein and three small peptides that all stay closely associated with the capsid (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei431 – 432Cleavage; by protease VP4By similarity2
Sitei474 – 475Cleavage; by protease VP4By similarity2
Sitei483 – 484Cleavage; by protease VP4By similarity2
Sitei500 – 501Cleavage; by protease VP4By similarity2
Sitei723 – 724Cleavage; by protease VP4By similarity2

Proteomic databases

PRIDEiQ96724

Interactioni

Subunit structurei

Capsid protein VP2 is a homotrimer. A central divalent metal stabilizes the VP2 trimer (By similarity). Capsid protein VP3 is a homodimer. Capsid protein VP3 interacts (via C-terminus) with VP1 in the cytoplasm. Capsid VP3 interacts with VP2 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ96724
SMRiQ96724
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini501 – 723Peptidase S50PROSITE-ProRule annotationAdd BLAST223

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1021 – 1030Interaction with VP1 proteinBy similarity10

Phylogenomic databases

OrthoDBiVOG0900008M

Family and domain databases

Gene3Di2.60.120.20, 2 hits
InterProiView protein in InterPro
IPR002662 Birna_VP2
IPR002663 Birna_VP3
IPR025775 Birna_VP4_Prtase_dom
IPR029053 Viral_coat
PfamiView protein in Pfam
PF01766 Birna_VP2, 1 hit
PF01767 Birna_VP3, 1 hit
PF01768 Birna_VP4, 1 hit
PROSITEiView protein in PROSITE
PS51548 BIRNAVIRUS_VP4_PRO, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTTNEYLKT LLNPAQFISD IPDDIMIRHV NSAQTITYNL KSGASGTGLI
60 70 80 90 100
VVYPNTPSSI SGFHYIWDSA TSNWVFDQYI YTAQELKDSY DYGRLISGSL
110 120 130 140 150
SIKSSTLPAG VYALNGTFNA VWFQGTLSEV SDYSYDRILS ITSNPLDKVG
160 170 180 190 200
NVLVGDGIEV LSLPQGFNNP YVRLGDKSPS TLSSPTHITN TSQNLATGGA
210 220 230 240 250
YMIPVTTVPG QGFHNKEFSI NVDSVGPVDI LWSGQMTMQD EWTVTANYQP
260 270 280 290 300
LNISGTLIAN SQRTLTWSNT GVSNGSHYMN MNNLNVSLFH ENPPPEPVAA
310 320 330 340 350
IKININYGNN TNGDSSFSVD SSFTINVIGG ATIGVNSPTV GVGYQGVAEG
360 370 380 390 400
TAITISGINN YELVPNPDLQ KNLPMTYGTC DPHDLTYIKY ILSNREQLGL
410 420 430 440 450
RSVMTLADYN RMKMYMHVLT NYHVDEREAS SFDFWQLLKQ IKNVAVPLAA
460 470 480 490 500
TLAPQFAPII GAADGLANAI LGDSASGRPV GNSASGMPIS MSRRLRNAYS
510 520 530 540 550
ADSPLGEEHW LPNENENFNK FDIIYDVSHS SMALFPVIMM EHDKVIPSDP
560 570 580 590 600
EELYIAVSLT ESLRKQIPNL NDMPYYEMGG HRVYNSVSSN VRSGNFLRSD
610 620 630 640 650
YILLPCYQLL EGRLASSTSP NKVTGTSHQL AIYAADDLLK SGVLGKAPFA
660 670 680 690 700
AFTGSVVGSS VGEVFGINLK LQLTDSLGIP LLGNSPGLVQ VKTLTSLDKK
710 720 730 740 750
IKDMGDVKRR TPKQTLPHWT AGSASMNPFM NTNPFLEELD QPIPSNAAKP
760 770 780 790 800
ISEETRDLFL SDGQTIPSSQ EKIATIHEYL LEHKELEEAM FSLISQGRGR
810 820 830 840 850
SLINMVVKSA LNIETQSREV TGERRQRLER KLRNLENQGI YVDESKIMSR
860 870 880 890 900
GRISKEDTEL AMRIARKNQK DAKLRRIYSN NASIQESYTV DDFVSYWMEQ
910 920 930 940 950
ESLPTGIQIA MWLKGDDWSQ PIPPRVQRRH YDSYIMMLGP SPTQEQADAV
960 970 980 990 1000
KDLVDDIYDR NQGKGPSQEQ ARELSHAVRR LISHSLVNQP ATAPRVPPRR
1010 1020 1030
IVSAQTAQTD PPGRRAALDR LRRVRGEDND IV
Length:1,032
Mass (Da):114,028
Last modified:February 1, 1997 - v1
Checksum:i44F5CB82FE191CFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60650 Genomic RNA Translation: AAB16798.1
RefSeqiNP_690836.1, NC_004177.1

Genome annotation databases

GeneIDi993338
KEGGivg:993338

Similar proteinsi

Entry informationi

Entry nameiPOLS_DXV96
AccessioniPrimary (citable) accession number: Q96724
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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