UniProtKB - Q965Q8 (XYLT_CAEEL)
Protein
Xylosyltransferase sqv-6
Gene
sqv-6
Organism
Caenorhabditis elegans
Status
Functioni
Catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. Required for vulval morphogenesis and zygotic cytokinesis, suggesting that glycosaminoglycans play a central role in vulval morphogenesis.1 Publication
Catalytic activityi
- EC:2.4.2.261 Publication
Cofactori
a divalent metal cationBy similarity
: chondroitin sulfate biosynthesis Pathwayi
This protein is involved in the pathway chondroitin sulfate biosynthesis, which is part of Glycan metabolism.View all proteins of this organism that are known to be involved in the pathway chondroitin sulfate biosynthesis and in Glycan metabolism.
Pathwayi: heparan sulfate biosynthesis
This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 264 | UDP-xyloseBy similarity | 1 | |
Binding sitei | 479 | UDP-xyloseBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- protein xylosyltransferase activity Source: WormBase
GO - Biological processi
- chondroitin sulfate biosynthetic process Source: WormBase
- chondroitin sulfate proteoglycan biosynthetic process Source: GO_Central
- heparan sulfate proteoglycan biosynthetic process Source: GO_Central
- heparin biosynthetic process Source: WormBase
- morphogenesis of an epithelium Source: WormBase
- oviposition Source: WormBase
- reproduction Source: WormBase
- UDP-D-xylose metabolic process Source: WormBase
- vulval development Source: WormBase
Keywordsi
Molecular function | Developmental protein, Glycosyltransferase, Transferase |
Ligand | Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.2.26, 1045 |
Reactomei | R-CEL-1971475, A tetrasaccharide linker sequence is required for GAG synthesis |
SignaLinki | Q965Q8 |
UniPathwayi | UPA00755 UPA00756 |
Protein family/group databases
CAZyi | GT14, Glycosyltransferase Family 14 |
Names & Taxonomyi
Protein namesi | Recommended name: Xylosyltransferase sqv-6 (EC:2.4.2.261 Publication)Alternative name(s): Peptide O-xylosyltransferase Squashed vulva protein 6 |
Gene namesi | Name:sqv-6 ORF Names:Y50D4C.4 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | Y50D4C.4a ; CE35919 ; WBGene00005024 ; sqv-6 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 32 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 33 – 806 | LumenalSequence analysisAdd BLAST | 774 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Worms are infertile because of the failure of the progeny of homozygous mutants to initiate cytokinesis and because of the failure to form an extracellular space between the egg and the eggshell.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191413 | 1 – 806 | Xylosyltransferase sqv-6Add BLAST | 806 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 57 ↔ 85 | By similarity | ||
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 101 ↔ 445 | By similarity | ||
Glycosylationi | 169 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 325 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 464 ↔ 478 | By similarity | ||
Disulfide bondi | 466 ↔ 476 | By similarity | ||
Glycosylationi | 614 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 655 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 719 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 772 ↔ 778 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q965Q8 |
PaxDbi | Q965Q8 |
PeptideAtlasi | Q965Q8 |
PTM databases
iPTMneti | Q965Q8 |
Expressioni
Gene expression databases
Bgeei | WBGene00005024, Expressed in adult organism and 4 other tissues |
ExpressionAtlasi | Q965Q8, baseline and differential |
Interactioni
Protein-protein interaction databases
BioGRIDi | 54695, 2 interactors |
STRINGi | 6239.Y50D4C.4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 109 – 205 | WSCPROSITE-ProRule annotationAdd BLAST | 97 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 293 – 295 | UDP-xylose bindingBy similarity | 3 | |
Regioni | 398 – 399 | UDP-xylose bindingBy similarity | 2 | |
Regioni | 505 – 506 | UDP-xylose bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0799, Eukaryota KOG4157, Eukaryota |
GeneTreei | ENSGT00940000169133 |
HOGENOMi | CLU_012840_1_0_1 |
InParanoidi | Q965Q8 |
OMAi | RMFRIGK |
OrthoDBi | 564384at2759 |
PhylomeDBi | Q965Q8 |
Family and domain databases
InterProi | View protein in InterPro IPR003406, Glyco_trans_14 IPR002889, WSC_carb-bd IPR043538, XYLT |
PANTHERi | PTHR46025, PTHR46025, 1 hit |
Pfami | View protein in Pfam PF02485, Branch, 1 hit PF01822, WSC, 1 hit |
SMARTi | View protein in SMART SM00321, WSC, 1 hit |
PROSITEi | View protein in PROSITE PS51212, WSC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q965Q8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLFNGTTKYR DYAIVISLFF LLNVYLLYNT AQHTQVGNSK HISSDSGEKT
60 70 80 90 100
SNPLPSCEIT DDLAKSAISR AITPSCKAKL QLEACQLKNG TFTINFPENQ
110 120 130 140 150
CPNHDSRLID QRIGCFLDKK EARVLTEFEY KLPKSNGKAT CRKHCYKAGF
160 170 180 190 200
LYFGLEFGHE CFCGNDVSNA TAVDDVECRA YKCPGNENSE EFCGGFNAVE
210 220 230 240 250
IFRTGFRSKV NHRKPTYLPP SSDSIKNPVK ILFLLQLNGR NERQVKRFLK
260 270 280 290 300
SIYLPHHYYY IHVDARQNYM FSEMQKVADF LDNIHITERR FSTIWGGASL
310 320 330 340 350
LQMFLQVIRD SMKIEKFKDW DYIINFSESD FPILPISDFE RLITVNNGKS
360 370 380 390 400
FLASHGYNTG KFIQKQGFEY VFSECDNRMF RIGKREFPQN LRIDGGSDWV
410 420 430 440 450
GIHRNLAEFS ISDEELPRKL RKTYESILLP LESFYHTLAF NSEFCDDLLM
460 470 480 490 500
SNLRLTNWYR KQGCRCASLK PIVDWCGCSP LVFREETMKK FELQKAISKP
510 520 530 540 550
TYFARKFDSM VDIDSIEAAE MQSISPEKLQ LNHPTYHFAF ANIFKTGIDE
560 570 580 590 600
QKLHFESLAN FALKSTETRA KFRKVLRIDA LRAHHNALIE IVMKIETTDG
610 620 630 640 650
ATFEFLIHRL SHVNLTENEE KLVEHGYLLR AVSFGTKFEW KEELCREYMG
660 670 680 690 700
FVTDNDTLHT RLQWHPTEHV KKVGDKTSPE MIFKYRKGDE LIEQTVVKPY
710 720 730 740 750
DSVFGGQFDS WNVGKKLSNL TTCSNFFVDI ISPSSPDDAP PLATLHFPVY
760 770 780 790 800
TDQNAHCHVD YLRQFFKIAD FCTSGDACKE KIWSTSYPDP KSDIFVGYDE
DTQTLI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0K3AYD4 | A0A0K3AYD4_CAEEL | Protein xylosyltransferase | sqv-6 CELE_Y50D4C.4, Y50D4C.4 | 428 | Annotation score: | ||
A0A0K3AT40 | A0A0K3AT40_CAEEL | Xylosyltransferase sqv-6 | sqv-6 CELE_Y50D4C.4, Y50D4C.4 | 126 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY241928 mRNA Translation: AAO85277.1 AJ496235 mRNA Translation: CAD42732.1 FO081564 Genomic DNA Translation: CCD72442.1 |
RefSeqi | NP_503359.2, NM_070958.3 |
Genome annotation databases
EnsemblMetazoai | Y50D4C.4a.1; Y50D4C.4a.1; WBGene00005024 Y50D4C.4a.2; Y50D4C.4a.2; WBGene00005024 |
GeneIDi | 190099 |
KEGGi | cel:CELE_Y50D4C.4 |
UCSCi | Y50D4C.4, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY241928 mRNA Translation: AAO85277.1 AJ496235 mRNA Translation: CAD42732.1 FO081564 Genomic DNA Translation: CCD72442.1 |
RefSeqi | NP_503359.2, NM_070958.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 54695, 2 interactors |
STRINGi | 6239.Y50D4C.4 |
Protein family/group databases
CAZyi | GT14, Glycosyltransferase Family 14 |
PTM databases
iPTMneti | Q965Q8 |
Proteomic databases
EPDi | Q965Q8 |
PaxDbi | Q965Q8 |
PeptideAtlasi | Q965Q8 |
Genome annotation databases
EnsemblMetazoai | Y50D4C.4a.1; Y50D4C.4a.1; WBGene00005024 Y50D4C.4a.2; Y50D4C.4a.2; WBGene00005024 |
GeneIDi | 190099 |
KEGGi | cel:CELE_Y50D4C.4 |
UCSCi | Y50D4C.4, c. elegans |
Organism-specific databases
CTDi | 190099 |
WormBasei | Y50D4C.4a ; CE35919 ; WBGene00005024 ; sqv-6 |
Phylogenomic databases
eggNOGi | KOG0799, Eukaryota KOG4157, Eukaryota |
GeneTreei | ENSGT00940000169133 |
HOGENOMi | CLU_012840_1_0_1 |
InParanoidi | Q965Q8 |
OMAi | RMFRIGK |
OrthoDBi | 564384at2759 |
PhylomeDBi | Q965Q8 |
Enzyme and pathway databases
UniPathwayi | UPA00755 UPA00756 |
BRENDAi | 2.4.2.26, 1045 |
Reactomei | R-CEL-1971475, A tetrasaccharide linker sequence is required for GAG synthesis |
SignaLinki | Q965Q8 |
Miscellaneous databases
PROi | PR:Q965Q8 |
Gene expression databases
Bgeei | WBGene00005024, Expressed in adult organism and 4 other tissues |
ExpressionAtlasi | Q965Q8, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR003406, Glyco_trans_14 IPR002889, WSC_carb-bd IPR043538, XYLT |
PANTHERi | PTHR46025, PTHR46025, 1 hit |
Pfami | View protein in Pfam PF02485, Branch, 1 hit PF01822, WSC, 1 hit |
SMARTi | View protein in SMART SM00321, WSC, 1 hit |
PROSITEi | View protein in PROSITE PS51212, WSC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XYLT_CAEEL | |
Accessioni | Q965Q8Primary (citable) accession number: Q965Q8 Secondary accession number(s): Q867S3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | March 1, 2004 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families