UniProtKB - Q96577 (Q96577_SOLLC)
Protein
Pectinesterase
Gene
LePME1
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Functioni
Acts in the modification of cell walls via demethylesterification of cell wall pectin.UniRule annotation
Catalytic activityi
- [(1→4)-α-D-galacturonosyl methyl ester](n) + n H2O = [(1→4)-α-D-galacturonosyl](n) + n H+ + n methanolUniRule annotationARBA annotationEC:3.1.1.11UniRule annotationARBA annotation
: pectin degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.UniRule annotationARBA annotationProteins known to be involved in the 5 steps of the subpathway in this organism are:
- Pectinesterase, Pectinesterase (101261415), Pectinesterase, Pectinesterase (101244818), Pectinesterase 2.1 (PME2.1), Pectinesterase (101246061), Pectinesterase, Pectinesterase (PMEU1), Pectinesterase 2.2 (PME2.2), Pectinesterase, Pectinesterase (101248642), Pectinesterase (101258375), Pectinesterase (LePME1), Pectinesterase 3 (PME3), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase 1 (PME1.9), Pectinesterase (101255775), Pectinesterase, Pectinesterase (101260091), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (101260282), Pectinesterase (101244677), Pectinesterase, Pectinesterase, Pectinesterase (101261269), Pectinesterase, Pectinesterase (101248083), Pectinesterase, Pectinesterase, Pectinesterase (101260787), Pectinesterase, Pectinesterase (101253120), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (101260795), Methionine aminopeptidase, Pectinesterase, Pectinesterase, Pectinesterase/pectinesterase inhibitor U1 (PMEU1), Pectinesterase (101268391), Pectinesterase (101247796), Pectinesterase (101260585), Pectinesterase (101260941), Pectinesterase (101247960), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (101266668), Pectinesterase, Pectinesterase (101262904), Pectinesterase (101261266), Pectinesterase, Pectinesterase (101268168), Pectinesterase (101244375), Pectinesterase, Pectinesterase, Pectinesterase (101261743), Pectinesterase (101257682), Pectinesterase, Pectinesterase (101245713), Pectinesterase (109119119), Pectinesterase, Pectinesterase (101264501), Pectinesterase, Pectinesterase (101266973), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase
- Pectate lyase (778293), Probable pectate lyase P18 (9612), Pectate lyase, Pectate lyase (101257960), Pectate lyase, Pectate lyase, Pectate lyase (101257697), Pectate lyase (101246909), Probable pectate lyase P59 (LAT59), Probable pectate lyase P56 (LAT56), Pectate lyase, Pectate lyase (101260096), Pectate lyase, Pectate lyase (101250914), Pectate lyase (101249735), Pectate lyase (101264933), Pectate lyase (101263458), Pectate lyase, Pectate lyase (101252082), Pectate lyase (101254639), Pectate lyase, Pectate lyase, Pectate lyase (101253459), Pectate lyase (101260911)
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 275 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- aspartyl esterase activity Source: UniProtKB-UniRule
- enzyme inhibitor activity Source: InterPro
- pectinesterase activity Source: UniProtKB-EC
GO - Biological processi
- cell wall modification Source: UniProtKB-UniRule
- pectin catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aspartyl esteraseUniRule annotationARBA annotation, Hydrolase |
Biological process | Cell wall biogenesis/degradationUniRule annotationARBA annotation |
Enzyme and pathway databases
UniPathwayi | UPA00545;UER00823 |
Names & Taxonomyi
Protein namesi | Recommended name: PectinesteraseUniRule annotationARBA annotation (EC:3.1.1.11UniRule annotationARBA annotation) |
Gene namesi | Name:LePME1Imported |
Organismi | Solanum lycopersicum (Tomato) (Lycopersicon esculentum)Imported |
Taxonomic identifieri | 4081 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Solanum subgen. Lycopersicon |
Subcellular locationi
Cell wall
- cell wall UniRule annotationARBA annotation
Cell Wall
- cell wall Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Keywords - Cellular componenti
Cell wallUniRule annotationARBA annotation, SecretedFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 77 | PMEIInterPro annotationAdd BLAST | 71 | |
Domaini | 127 – 422 | PectinesteraseInterPro annotationAdd BLAST | 296 |
Sequence similaritiesi
In the C-terminal section; belongs to the pectinesterase family.ARBA annotation
In the N-terminal section; belongs to the PMEI family.ARBA annotation
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit |
SUPFAMi | SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit |
i Sequence
Sequence statusi: Fragment.
Q96577-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
NDIRQHGALT DCLELLDQSV DLASDSIAAI DKRSRSEHAN SESWLSGVLT
60 70 80 90 100
NHVTCLDELD SFTKAMINGT NLEELISRAK VALAMLASLT TQDEDVFMTG
110 120 130 140 150
LGKMPSWVSS MDRKLMESSG KDIIANRVVA QDGTGKYRTL AEAVAAAPNR
160 170 180 190 200
SKKRYVIYVK RGIYKENVEV SSNKMNLMIV GDGMYATTIT GSLNVVDGST
210 220 230 240 250
TFRSATLAAV GQGFILQDIC IQNTAGPAKD QAVALRVGAD MSVINRCRID
260 270 280 290 300
AYQDTLYAHS QRQFYRDSYV TGTVDFIFGN AAVVFQKCQL VARKPGKYQQ
310 320 330 340 350
NMVTAQGTTD PNQATGTSIQ FCNIIASSDL EPVLKEFPTY LGRPWKEYSR
360 370 380 390 400
TVVMESYLGG LINPAGWAEW DGDFALKTLY YGEFMNNGPG AGTSKRVKWP
410 420 430
GYHVITDPAK AMPFTVAKLI QGGSWLRSTG VAYVDGLYD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | Imported | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70677 Genomic DNA Translation: AAB38794.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70677 Genomic DNA Translation: AAB38794.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Enzyme and pathway databases
UniPathwayi | UPA00545;UER00823 |
Gene expression databases
ExpressionAtlasi | Q96577, baseline and differential |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit |
SUPFAMi | SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q96577_SOLLC | |
Accessioni | Q96577Primary (citable) accession number: Q96577 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | February 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |