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Protein

Pectinesterase 2.2

Gene

PME2.2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Pectinesterase may play a role in cell wall metabolism during fruit growth and development prior to ripening and may be required for preparing cell walls for softening by polygalacturonase during fruit ripening.

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (101260834), Pectinesterase, Pectinesterase (101247960), Pectinesterase (101261266), Pectinesterase, Pectinesterase (101245743), Pectinesterase, Pectinesterase (544016), Pectinesterase (101260795), Pectinesterase (101247796), Pectinesterase (101262904), Pectinesterase (101266668), Pectinesterase, Pectinesterase (101257682), Pectinesterase (101244375), Pectinesterase (101261415), Pectinesterase, Pectinesterase (101260091), Pectinesterase (101268487), Pectinesterase, Pectinesterase (104645850), Pectinesterase (101246061), Pectinesterase (101260941), Pectinesterase (101260787), Pectinesterase (101255817), Pectinesterase (101245115), Uncharacterized protein, Pectinesterase (101253120), Pectinesterase (101268168), Pectinesterase (109119119), Pectinesterase (101264501), Pectinesterase (101245713), Pectinesterase, Pectinesterase (101260197), Pectinesterase (101245951), Pectinesterase, Pectinesterase (101244818), Pectinesterase (101260471), Pectinesterase, Pectinesterase (101244677), Pectinesterase (101248083), Pectinesterase (101266973), Pectinesterase (101248642), Pectinesterase (LePME1), Pectinesterase, Pectinesterase, Pectinesterase (101255775), Pectinesterase (101258375), Pectinesterase (101253971), Pectinesterase (101261743), Pectinesterase (101263475), Pectinesterase (PMEU1), Pectinesterase 1 (PME1.9), Pectinesterase 2.1 (PME2.1), Pectinesterase 2.2 (PME2.2), Pectinesterase 3 (PME3), Pectinesterase/pectinesterase inhibitor U1 (PMEU1)
  2. Pectate lyase (101263458), Pectate lyase (101260096), Pectate lyase, Pectate lyase (101267397), Pectate lyase (101264933), Pectate lyase (101246909), Pectate lyase, Pectate lyase, Pectate lyase (101254639), Pectate lyase (778293), Pectate lyase (101253459), Pectate lyase (101244298), Pectate lyase (101257960), Pectate lyase, Pectate lyase (101257697), Pectate lyase (101260911), Pectate lyase (101250914), Pectate lyase (101254471), Pectate lyase, Pectate lyase, Pectate lyase (101252082), Pectate lyase (101249735), Probable pectate lyase P18 (9612), Probable pectate lyase P56 (LAT56), Probable pectate lyase P59 (LAT59)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei312SubstrateBy similarity1
Binding sitei342SubstrateBy similarity1
Sitei364Transition state stabilizerBy similarity1
Active sitei365Proton donorPROSITE-ProRule annotation1
Active sitei386NucleophilePROSITE-ProRule annotation1
Binding sitei454SubstrateBy similarity1
Binding sitei456SubstrateBy similarity1

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: GO_Central
  • pectinesterase inhibitor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation, Fruit ripening

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 2.2 (EC:3.1.1.11)
Short name:
PE 2.2
Alternative name(s):
Pectin methylesterase 2.2
Gene namesi
Name:PME2.2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000023490? – 233Sequence analysis
Signal peptidei1 – ?Sequence analysis
ChainiPRO_0000023491234 – 550Pectinesterase 2.2Add BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi179N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi331 ↔ 358By similarity
Disulfide bondi399 ↔ 433By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ96575

Expressioni

Gene expression databases

ExpressionAtlasiQ96575 baseline and differential

Structurei

3D structure databases

ProteinModelPortaliQ96575
SMRiQ96575
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ96575

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
IPR018040 Pectinesterase_Tyr_AS
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00800 PECTINESTERASE_1, 1 hit
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96575-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATPQQPLLT KTHKQNSIIS FKILTFVVTL FVALFLVVFL VAPYQFEIKH
60 70 80 90 100
SNLCKTAQDS QLCLSYVSDL MSNEIVTTDS DGLSILMKFL VNYVHQMNNA
110 120 130 140 150
IPVVSKMKNQ INDIRQEGAL TDCLELLDQS VDLVSDSIAA IDKRTHSEHA
160 170 180 190 200
NAQSWLSGVL TNHVTCLDEL DSFTKAMING TNLDELISRA KVALAMLASV
210 220 230 240 250
TTPNDDVLRP GLGKMPSWVS SRDRKLMESS GKDIGANAVV AKDGTGKYRT
260 270 280 290 300
LAEAVAAAPD KSKTRYVIYV KRGIYKENVE VSSRKMKLMI VGDGMHATII
310 320 330 340 350
TGNLNVVDGS TTFHSATLAA VGKGFILQDI CIQNTAGPAK HQAVALRVGA
360 370 380 390 400
DKSVINRCRI DAYQDTLYAH SQRQFYRDSY VTGTIDFIFG NAAVVFQKCK
410 420 430 440 450
LVARKPGKYQ QNMVTAQGRT DPNQATGTSI QFCNIIASSD LEPVLKEFPT
460 470 480 490 500
YLGRPWKKYS RTVVMESYLG GLINPAGWAE WDGDFALKTL YYGEFMNNGP
510 520 530 540 550
GAGTSKRVKW PGYHCITDPA EAMPFTVAKL IQGGSWLRST GVAYVDGLYD
Length:550
Mass (Da):60,425
Last modified:February 1, 1997 - v1
Checksum:iBF95179FAD94ED51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70675 Genomic DNA Translation: AAB38792.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70675 Genomic DNA Translation: AAB38792.1

3D structure databases

ProteinModelPortaliQ96575
SMRiQ96575
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ96575

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiQ96575

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

Gene expression databases

ExpressionAtlasiQ96575 baseline and differential

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
IPR018040 Pectinesterase_Tyr_AS
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00800 PECTINESTERASE_1, 1 hit
PS00503 PECTINESTERASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPME22_SOLLC
AccessioniPrimary (citable) accession number: Q96575
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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