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UniProtKB - Q96562 (CHS2_HORVU)
Protein
Chalcone synthase 2
Gene
CHS2
Organism
Hordeum vulgare (Barley)
Status
Functioni
The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin. Substrate preference is feruloyl-CoA = caffeoyl-CoA >> cinnamoyl-CoA.
Catalytic activityi
- 4-coumaroyl-CoA + 2 H+ + 3 malonyl-CoA = 2',4,4',6'-tetrahydroxychalcone + 3 CO2 + 4 CoAPROSITE-ProRule annotationEC:2.3.1.74PROSITE-ProRule annotation
: flavonoid biosynthesis Pathwayi
This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 166 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- chalcone synthase activity Source: UniProtKB-EC
- naringenin-chalcone synthase activity Source: UniProtKB-EC
GO - Biological processi
- flavonoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Flavonoid biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00154 |
Names & Taxonomyi
Protein namesi | Recommended name: Chalcone synthase 2 (EC:2.3.1.74)Alternative name(s): Naringenin-chalcone synthase 2 |
Gene namesi | Name:CHS2 |
Organismi | Hordeum vulgare (Barley) |
Taxonomic identifieri | 4513 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Pooideae › Triticodae › Triticeae › Hordeinae › Hordeum |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215980 | 1 – 399 | Chalcone synthase 2Add BLAST | 399 |
Expressioni
Inductioni
Strong in response to inoculation with fungi.
Gene expression databases
ExpressionAtlasi | Q96562, baseline and differential |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR012328, Chalcone/stilbene_synt_C IPR001099, Chalcone/stilbene_synt_N IPR018088, Chalcone/stilbene_synthase_AS IPR011141, Polyketide_synthase_type-III IPR016039, Thiolase-like |
PANTHERi | PTHR11877, PTHR11877, 1 hit |
Pfami | View protein in Pfam PF02797, Chal_sti_synt_C, 1 hit PF00195, Chal_sti_synt_N, 1 hit |
PIRSFi | PIRSF000451, PKS_III, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
PROSITEi | View protein in PROSITE PS00441, CHALCONE_SYNTH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q96562-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAVRLKEVR MAQRAEGLAT VLAIGTAVPA NCVYQATYPD YYFRVTKSEH
60 70 80 90 100
LADLKEKFQR MCDKSMIRKR HMHLTEEILI KNPKICAHME TSLDARHAIA
110 120 130 140 150
LVEVPKLGQG AAEKAIKEWG QPLSKITHLV FCTTSGVDMP GADYQLTKLL
160 170 180 190 200
GLSPTVKRLM MYQQGCFGGA TVLRLAKDIA ENNRGARVLV VCSEITAMAF
210 220 230 240 250
RGPCKSHLDS LVGHALFGDG AAAAIIGADP DQLDEQPVFQ LVSASQTILP
260 270 280 290 300
ESEGAIDGHL TEAGLTIHLL KDVPGLISEN IEQALEDAFE PLGIHNWNSI
310 320 330 340 350
FWIAHPGGPA ILDRVEDRVG LDKKRMRASR EVLSEYGNMS SASVLFVLDV
360 370 380 390
MRKSSAKDGL ATTGEGKDWG VLFGFGPGLT VETLVLHSVP VPVPTAASA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y09233 mRNA Translation: CAA70435.1 |
PIRi | T04485 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y09233 mRNA Translation: CAA70435.1 |
PIRi | T04485 |
3D structure databases
AlphaFoldDBi | Q96562 |
SMRi | Q96562 |
ModBasei | Search... |
Genome annotation databases
Enzyme and pathway databases
UniPathwayi | UPA00154 |
Gene expression databases
ExpressionAtlasi | Q96562, baseline and differential |
Family and domain databases
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR012328, Chalcone/stilbene_synt_C IPR001099, Chalcone/stilbene_synt_N IPR018088, Chalcone/stilbene_synthase_AS IPR011141, Polyketide_synthase_type-III IPR016039, Thiolase-like |
PANTHERi | PTHR11877, PTHR11877, 1 hit |
Pfami | View protein in Pfam PF02797, Chal_sti_synt_C, 1 hit PF00195, Chal_sti_synt_N, 1 hit |
PIRSFi | PIRSF000451, PKS_III, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
PROSITEi | View protein in PROSITE PS00441, CHALCONE_SYNTH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHS2_HORVU | |
Accessioni | Q96562Primary (citable) accession number: Q96562 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | February 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families