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UniProtKB - Q96533 (ADHX_ARATH)
Protein
Alcohol dehydrogenase class-3
Gene
ADH2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Plays a central role in formaldehyde detoxification.
1 PublicationCatalytic activityi
- EC:1.1.1.12 Publications
- EC:1.1.1.12 Publications
- EC:1.1.1.2842 Publications
- EC:1.1.1.2842 Publications
Cofactori
Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication
Kineticsi
kcat is 1351 min(-1) with S-hydroxymethylglutathione as substrate (at pH 8 and 25 degrees Celsius). kcat is 6 min(-1) with farnesol as substrate (at pH 7.5 and 25 degrees Celsius). kcat is 324 min(-1) with cinnamylalcohol as substrate (at pH 7.5 and 25 degrees Celsius). kcat is 126 min(-1) with farnesol as substrate (at pH 10 and 25 degrees Celsius). kcat is 1200 min(-1) with geraniol as substrate (at pH 10 and 25 degrees Celsius). kcat is 1220 min(-1) with cinnamylalcohol as substrate (at pH 10 and 25 degrees Celsius). kcat is 335 min(-1) with 12-Hydroxydodecanoic acid as substrate (at pH 10 and 25 degrees Celsius).1 Publication
- KM=1.4 µM for S-(hydroxymethyl)glutathione1 Publication
- KM=7 µM for S-hydroxymethylglutathione (at pH 8 and 25 degrees Celsius)1 Publication
- KM=7.7 µM for farnesol (at pH 7.5 and 25 degrees Celsius)1 Publication
- KM=22000 µM for cinnamylalcohol (at pH 7.5 and 25 degrees Celsius)1 Publication
- KM=3 µM for farnesol (at pH 10 and 25 degrees Celsius)1 Publication
- KM=800 µM for geraniol (at pH 10 and 25 degrees Celsius)1 Publication
- KM=3500 µM for cinnamylalcohol (at pH 10 and 25 degrees Celsius)1 Publication
- KM=4700 µM for 12-Hydroxydodecanoic acid (at pH 10 and 25 degrees Celsius)1 Publication
- Vmax=1.22 µmol/min/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | Zinc 1; catalyticCombined sources1 Publication | 1 | |
Binding sitei | 48 | NADCombined sources1 Publication | 1 | |
Binding sitei | 49 | SubstrateBy similarity | 1 | |
Metal bindingi | 69 | Zinc 1; catalyticCombined sources1 Publication | 1 | |
Binding sitei | 69 | SubstrateBy similarity | 1 | |
Metal bindingi | 70 | Zinc 1Combined sources1 Publication | 1 | |
Metal bindingi | 99 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 102 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 105 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 113 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 177 | Zinc 1; catalyticCombined sources1 Publication | 1 | |
Binding sitei | 226 | NADCombined sources1 Publication | 1 | |
Binding sitei | 231 | NADCombined sources1 Publication | 1 | |
Binding sitei | 272 | NAD; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 372 | NADCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 202 – 207 | NADCombined sources1 Publication | 6 | |
Nucleotide bindingi | 295 – 297 | NADCombined sources1 Publication | 3 | |
Nucleotide bindingi | 320 – 322 | NADCombined sources1 Publication | 3 |
GO - Molecular functioni
- alcohol dehydrogenase activity, zinc-dependent Source: GO_Central
- S-(hydroxymethyl)glutathione dehydrogenase activity Source: GO_Central
- S-(hydroxymethyl)glutathione dehydrogenase NAD activity Source: UniProtKB-EC
- S-(hydroxymethyl)glutathione dehydrogenase NADP activity Source: UniProtKB-EC
- zinc ion binding Source: GO_Central
GO - Biological processi
- ethanol oxidation Source: InterPro
- formaldehyde catabolic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Enzyme and pathway databases
BioCyci | ARA:AT5G43940-MONOMER |
SABIO-RKi | Q96533 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ADH2 Synonyms:ADHIII, FDH1 Ordered Locus Names:At5g43940 ORF Names:MRH10.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G43940 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000160771 | 2 – 379 | Alcohol dehydrogenase class-3Add BLAST | 378 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q96533 |
PRIDEi | Q96533 |
ProteomicsDBi | 244654 [Q96533-1] |
PTM databases
iPTMneti | Q96533 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Inductioni
Down-regulated by wounding and activated by salicylic acid (SA).1 Publication
Gene expression databases
ExpressionAtlasi | Q96533, baseline and differential |
Genevisiblei | Q96533, AT |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 19667, 4 interactors |
STRINGi | 3702.AT5G43940.2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96533 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0022, Eukaryota |
HOGENOMi | CLU_026673_14_0_1 |
InParanoidi | Q96533 |
OMAi | CIRCVVN |
PhylomeDBi | Q96533 |
Family and domain databases
CDDi | cd08300, alcohol_DH_class_III, 1 hit |
InterProi | View protein in InterPro IPR014183, ADH_3 IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02818, adh_III_F_hyde, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96533-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATQGQVITC KAAVAYEPNK PLVIEDVQVA PPQAGEVRIK ILYTALCHTD
60 70 80 90 100
AYTWSGKDPE GLFPCILGHE AAGIVESVGE GVTEVQAGDH VIPCYQAECR
110 120 130 140 150
ECKFCKSGKT NLCGKVRSAT GVGIMMNDRK SRFSVNGKPI YHFMGTSTFS
160 170 180 190 200
QYTVVHDVSV AKIDPTAPLD KVCLLGCGVP TGLGAVWNTA KVEPGSNVAI
210 220 230 240 250
FGLGTVGLAV AEGAKTAGAS RIIGIDIDSK KYETAKKFGV NEFVNPKDHD
260 270 280 290 300
KPIQEVIVDL TDGGVDYSFE CIGNVSVMRA ALECCHKGWG TSVIVGVAAS
310 320 330 340 350
GQEISTRPFQ LVTGRVWKGT AFGGFKSRTQ VPWLVEKYMN KEIKVDEYIT
360 370
HNLTLGEINK AFDLLHEGTC LRCVLDTSK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4K7D6 | F4K7D6_ARATH | S-(hydroxymethyl)glutathione dehydr... | HOT5 ADH2, ALCOHOL DEHYDROGENASE 2, ATGSNOR1, FALDH, GSNOR | 391 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | E → G in AAB06322 (PubMed:9215914).Curated | 1 | |
Sequence conflicti | 354 | T → S in CAA57973 (PubMed:8944774).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63931 Genomic DNA Translation: AAB06322.1 X82647 mRNA Translation: CAA57973.1 AB006703 Genomic DNA Translation: BAB09054.1 CP002688 Genomic DNA Translation: AED95034.1 AY039601 mRNA Translation: AAK62656.1 BT010169 mRNA Translation: AAQ22638.1 AK226412 mRNA Translation: BAE98557.1 AY087250 mRNA Translation: AAM64806.1 |
PIRi | S71244 |
RefSeqi | NP_199207.1, NM_123761.4 [Q96533-1] |
Genome annotation databases
EnsemblPlantsi | AT5G43940.1; AT5G43940.1; AT5G43940 [Q96533-1] |
GeneIDi | 834417 |
Gramenei | AT5G43940.1; AT5G43940.1; AT5G43940 [Q96533-1] |
KEGGi | ath:AT5G43940 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63931 Genomic DNA Translation: AAB06322.1 X82647 mRNA Translation: CAA57973.1 AB006703 Genomic DNA Translation: BAB09054.1 CP002688 Genomic DNA Translation: AED95034.1 AY039601 mRNA Translation: AAK62656.1 BT010169 mRNA Translation: AAQ22638.1 AK226412 mRNA Translation: BAE98557.1 AY087250 mRNA Translation: AAM64806.1 |
PIRi | S71244 |
RefSeqi | NP_199207.1, NM_123761.4 [Q96533-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3UKO | X-ray | 1.40 | A/B | 2-379 | [»] | |
4GL4 | X-ray | 1.80 | A/B | 2-379 | [»] | |
4JJI | X-ray | 1.80 | A/B | 2-379 | [»] | |
4L0Q | X-ray | 1.95 | A/B | 2-379 | [»] | |
SMRi | Q96533 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 19667, 4 interactors |
STRINGi | 3702.AT5G43940.2 |
PTM databases
iPTMneti | Q96533 |
Proteomic databases
PaxDbi | Q96533 |
PRIDEi | Q96533 |
ProteomicsDBi | 244654 [Q96533-1] |
Genome annotation databases
EnsemblPlantsi | AT5G43940.1; AT5G43940.1; AT5G43940 [Q96533-1] |
GeneIDi | 834417 |
Gramenei | AT5G43940.1; AT5G43940.1; AT5G43940 [Q96533-1] |
KEGGi | ath:AT5G43940 |
Organism-specific databases
Araporti | AT5G43940 |
Phylogenomic databases
eggNOGi | KOG0022, Eukaryota |
HOGENOMi | CLU_026673_14_0_1 |
InParanoidi | Q96533 |
OMAi | CIRCVVN |
PhylomeDBi | Q96533 |
Enzyme and pathway databases
BioCyci | ARA:AT5G43940-MONOMER |
SABIO-RKi | Q96533 |
Miscellaneous databases
PROi | PR:Q96533 |
Gene expression databases
ExpressionAtlasi | Q96533, baseline and differential |
Genevisiblei | Q96533, AT |
Family and domain databases
CDDi | cd08300, alcohol_DH_class_III, 1 hit |
InterProi | View protein in InterPro IPR014183, ADH_3 IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02818, adh_III_F_hyde, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ADHX_ARATH | |
Accessioni | Q96533Primary (citable) accession number: Q96533 Secondary accession number(s): Q0WWE1, Q43384, Q9FND2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | March 27, 2002 | |
Last modified: | February 23, 2022 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families