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Protein

Leucoanthocyanidin dioxygenase

Gene

LDOX

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in anthocyanin and protoanthocyanidin biosynthesis by catalyzing the oxidation of leucoanthocyanidins into anthocyanidins. Possesses low flavonol synthase activity in vitro towards dihydrokaempferol and dihydroquercetin producing kaempferol and quercitin, respectively.5 Publications

Catalytic activityi

A (2R,3S,4S)-leucoanthocyanidin + 2-oxoglutarate + O2 = an anthocyanidin + succinate + CO2 + 2 H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142Substrate2 Publications1
Binding sitei213Substrate2 Publications1
Metal bindingi232Iron; catalytic2 Publications1
Binding sitei233Substrate2 Publications1
Metal bindingi234Iron; catalytic2 Publications1
Metal bindingi288Iron; catalytic2 Publications1
Binding sitei306Substrate2 Publications1
Binding sitei341Substrate2 Publications1

GO - Molecular functioni

  • L-ascorbic acid binding Source: UniProtKB-KW
  • leucocyanidin oxygenase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • anthocyanin-containing compound biosynthetic process Source: TAIR
  • proanthocyanidin biosynthetic process Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: TAIR
  • vacuole organization Source: TAIR

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFlavonoid biosynthesis
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

BioCyciARA:AT4G22880-MONOMER
MetaCyc:AT4G22880-MONOMER
UniPathwayi
UPA00009

Names & Taxonomyi

Protein namesi
Recommended name:
Leucoanthocyanidin dioxygenase (EC:1.14.20.41 Publication)
Short name:
LDOX
Short name:
Leucocyanidin oxygenase
Alternative name(s):
Anthocyanidin synthase
Short name:
ANS
Leucoanthocyanidin hydroxylase
Protein TANNIN DEFICIENT SEED 4
Short name:
TDS4
Protein TRANSPARENT TESTA 11
Short name:
TT11
Protein TRANSPARENT TESTA 17
Short name:
TT17
Protein TRANSPARENT TESTA 18
Short name:
TT18
Gene namesi
Name:LDOX
Ordered Locus Names:At4g22880
ORF Names:F7H19.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G22880
TAIRilocus:2127218 AT4G22880

Pathology & Biotechi

Disruption phenotypei

No accumulation of anthocyanins, accumulation of protoanthocyanidin intermediates and presence of numerous small vacuoles in leaf epidermal cells.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78G → E in tt11-2; no accumulation of anthocyanin. 1 Publication1
Mutagenesisi128K → A: Retains two-third of the original activity. 1 Publication1
Mutagenesisi131N → A or D: Retains two-third of the original activity. 1 Publication1
Mutagenesisi142Y → H: Retains two-third of the original activity. 1 Publication1
Mutagenesisi220C → Y in tt17; no accumulation of anthocyanin. 1 Publication1
Mutagenesisi228G → D in tds4-1; no accumulation of anthocyanin. 2 Publications1
Mutagenesisi230E → Q: Retains one half of the original activity. 1 Publication1
Mutagenesisi341K → N: Retains two-third of the original activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000672991 – 356Leucoanthocyanidin dioxygenaseAdd BLAST356

Proteomic databases

PaxDbiQ96323

PTM databases

iPTMnetiQ96323

Expressioni

Tissue specificityi

Expressed in young seedlings (at protein level).1 Publication

Inductioni

By methyl jasmonate, 6-benzylaminopurine, light and sucrose.3 Publications

Gene expression databases

ExpressionAtlasiQ96323 baseline and differential
GenevisibleiQ96323 AT

Interactioni

Protein-protein interaction databases

BioGridi13676, 1 interactor
STRINGi3702.AT4G22880.1

Structurei

Secondary structure

1356
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ96323
SMRiQ96323
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96323

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 307Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 2172-oxoglutarate binding2 Publications3
Regioni298 – 3002-oxoglutarate binding2 Publications3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0143 Eukaryota
COG3491 LUCA
HOGENOMiHOG000276735
InParanoidiQ96323
KOiK05277
OMAiGTIIVHV
OrthoDBiEOG09360F9L
PhylomeDBiQ96323

Family and domain databases

Gene3Di2.60.120.330, 1 hit
InterProiView protein in InterPro
IPR026992 DIOX_N
IPR027443 IPNS-like
IPR005123 Oxoglu/Fe-dep_dioxygenase
PfamiView protein in Pfam
PF03171 2OG-FeII_Oxy, 1 hit
PF14226 DIOX_N, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

Sequencei

Sequence statusi: Complete.

Q96323-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVAVERVESL AKSGIISIPK EYIRPKEELE SINDVFLEEK KEDGPQVPTI
60 70 80 90 100
DLKNIESDDE KIRENCIEEL KKASLDWGVM HLINHGIPAD LMERVKKAGE
110 120 130 140 150
EFFSLSVEEK EKYANDQATG KIQGYGSKLA NNASGQLEWE DYFFHLAYPE
160 170 180 190 200
EKRDLSIWPK TPSDYIEATS EYAKCLRLLA TKVFKALSVG LGLEPDRLEK
210 220 230 240 250
EVGGLEELLL QMKINYYPKC PQPELALGVE AHTDVSALTF ILHNMVPGLQ
260 270 280 290 300
LFYEGKWVTA KCVPDSIVMH IGDTLEILSN GKYKSILHRG LVNKEKVRIS
310 320 330 340 350
WAVFCEPPKD KIVLKPLPEM VSVESPAKFP PRTFAQHIEH KLFGKEQEEL

VSEKND
Length:356
Mass (Da):40,396
Last modified:February 1, 1997 - v1
Checksum:i1B74AE3A54056201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70478 mRNA Translation: AAB09572.1
AL031018 Genomic DNA Translation: CAA19803.1
AL161558 Genomic DNA Translation: CAB79243.1
CP002687 Genomic DNA Translation: AEE84672.1
CP002687 Genomic DNA Translation: AEE84673.1
CP002687 Genomic DNA Translation: ANM67022.1
AY088203 mRNA Translation: AAM65745.1
PIRiT05119
RefSeqiNP_001031700.1, NM_001036623.1
NP_001320035.1, NM_001341563.1
NP_194019.1, NM_118417.2
UniGeneiAt.2369

Genome annotation databases

EnsemblPlantsiAT4G22880.1; AT4G22880.1; AT4G22880
AT4G22880.2; AT4G22880.2; AT4G22880
AT4G22880.3; AT4G22880.3; AT4G22880
GeneIDi828387
GrameneiAT4G22880.1; AT4G22880.1; AT4G22880
AT4G22880.2; AT4G22880.2; AT4G22880
AT4G22880.3; AT4G22880.3; AT4G22880
KEGGiath:AT4G22880

Similar proteinsi

Entry informationi

Entry nameiLDOX_ARATH
AccessioniPrimary (citable) accession number: Q96323
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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