UniProtKB - Q96291 (BAS1A_ARATH)
Protein
2-Cys peroxiredoxin BAS1, chloroplastic
Gene
BAS1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf.1 Publication
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin CDSP32.1 Publication
Catalytic activityi
- EC:1.11.1.241 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 119 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- peroxidase activity Source: UniProtKB
- peroxiredoxin activity Source: TAIR
- thioredoxin peroxidase activity Source: GO_Central
GO - Biological processi
- cell redox homeostasis Source: TAIR
- defense response to bacterium Source: TAIR
- response to cold Source: TAIR
- response to oxidative stress Source: GO_Central
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BioCyci | ARA:AT3G11630-MONOMER |
BRENDAi | 1.11.1.15, 399 |
Protein family/group databases
PeroxiBasei | 4358, At2CysPrx01 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-Cys peroxiredoxin BAS1, chloroplastic (EC:1.11.1.241 Publication)Short name: 2-Cys Prx A Short name: 2-Cys peroxiredoxin A Alternative name(s): Thiol-specific antioxidant protein A Thioredoxin-dependent peroxiredoxin BAS1Curated |
Gene namesi | Name:BAS1 Ordered Locus Names:At3g11630 ORF Names:F24K9.28, T19F11.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G11630 |
TAIRi | locus:2080707, AT3G11630 |
Subcellular locationi
Chloroplast
- chloroplast 1 Publication
Chloroplast
- chloroplast Source: TAIR
- chloroplast envelope Source: TAIR
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: TAIR
Extracellular region or secreted
- apoplast Source: TAIR
Other locations
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 65 | ChloroplastBy similarityAdd BLAST | 65 | |
ChainiPRO_0000023784 | 66 – 266 | 2-Cys peroxiredoxin BAS1, chloroplasticAdd BLAST | 201 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 119 | Interchain (with C-241); in linked formBy similarity | ||
Disulfide bondi | 241 | Interchain (with C-119); in linked formBy similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | Q96291 |
PRIDEi | Q96291 |
ProteomicsDBi | 241127 |
PTM databases
iPTMneti | Q96291 |
MetOSitei | Q96291 |
Expressioni
Inductioni
Down-regulated under highly reduced cellular thiol pool conditions. Down-regulated by ascorbate. Slightly induced by oxidative stress.1 Publication
Gene expression databases
ExpressionAtlasi | Q96291, baseline and differential |
Genevisiblei | Q96291, AT |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 5669, 11 interactors |
IntActi | Q96291, 8 interactors |
MINTi | Q96291 |
STRINGi | 3702.AT3G11630.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q96291 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 73 – 232 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 160 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Transit peptidePhylogenomic databases
eggNOGi | KOG0852, Eukaryota |
HOGENOMi | CLU_042529_21_0_1 |
InParanoidi | Q96291 |
OMAi | WWPKDFT |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q96291 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q96291-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASVASSTTL ISSPSSRVFP AKSSLSSPSV SFLRTLSSPS ASASLRSGFA
60 70 80 90 100
RRSSLSSTSR RSFAVKAQAD DLPLVGNKAP DFEAEAVFDQ EFIKVKLSDY
110 120 130 140 150
IGKKYVILFF YPLDFTFVCP TEITAFSDRH SEFEKLNTEV LGVSVDSVFS
160 170 180 190 200
HLAWVQTDRK SGGLGDLNYP LISDVTKSIS KSFGVLIHDQ GIALRGLFII
210 220 230 240 250
DKEGVIQHST INNLGIGRSV DETMRTLQAL QYIQENPDEV CPAGWKPGEK
260
SMKPDPKLSK EYFSAI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 83 | E → K in CAA63909 (Ref. 1) Curated | 1 | |
Sequence conflicti | 83 | E → K in CAA71503 (Ref. 1) Curated | 1 | |
Sequence conflicti | 101 | I → N (Ref. 1) Curated | 1 | |
Sequence conflicti | 101 | I → N (PubMed:9263459).Curated | 1 | |
Sequence conflicti | 122 | Missing in CAA66484 (Ref. 1) Curated | 1 | |
Sequence conflicti | 174 – 175 | DV → YF in AAM64537 (Ref. 7) Curated | 2 | |
Sequence conflicti | 192 | I → IGI (PubMed:9263459).Curated | 1 | |
Sequence conflicti | 218 | R → Q in AAM64537 (Ref. 7) Curated | 1 | |
Sequence conflicti | 233 – 235 | IQE → TG in CAA63909 (Ref. 1) Curated | 3 | |
Sequence conflicti | 233 – 235 | IQE → TG in CAA71503 (Ref. 1) Curated | 3 | |
Sequence conflicti | 247 | P → S in CAA63909 (Ref. 1) Curated | 1 | |
Sequence conflicti | 247 | P → S in CAA71503 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94218 mRNA Translation: CAA63909.1 Y10478 mRNA Translation: CAA71503.1 X97910 Genomic DNA Translation: CAA66484.2 AC008153 Genomic DNA Translation: AAG51430.1 AC009918 Genomic DNA Translation: AAF02131.1 CP002686 Genomic DNA Translation: AEE75077.1 AF324996 mRNA Translation: AAG40348.1 AF419578 mRNA Translation: AAL31910.1 AY079107 mRNA Translation: AAL84991.1 AY086974 mRNA Translation: AAM64537.1 |
RefSeqi | NP_187769.1, NM_111995.3 |
Genome annotation databases
EnsemblPlantsi | AT3G11630.1; AT3G11630.1; AT3G11630 |
GeneIDi | 820335 |
Gramenei | AT3G11630.1; AT3G11630.1; AT3G11630 |
KEGGi | ath:AT3G11630 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94218 mRNA Translation: CAA63909.1 Y10478 mRNA Translation: CAA71503.1 X97910 Genomic DNA Translation: CAA66484.2 AC008153 Genomic DNA Translation: AAG51430.1 AC009918 Genomic DNA Translation: AAF02131.1 CP002686 Genomic DNA Translation: AEE75077.1 AF324996 mRNA Translation: AAG40348.1 AF419578 mRNA Translation: AAL31910.1 AY079107 mRNA Translation: AAL84991.1 AY086974 mRNA Translation: AAM64537.1 |
RefSeqi | NP_187769.1, NM_111995.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ZTE | X-ray | 2.60 | A/B/C/D/E/F/G/H/I/J | 73-266 | [»] | |
SMRi | Q96291 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 5669, 11 interactors |
IntActi | Q96291, 8 interactors |
MINTi | Q96291 |
STRINGi | 3702.AT3G11630.1 |
Protein family/group databases
PeroxiBasei | 4358, At2CysPrx01 |
PTM databases
iPTMneti | Q96291 |
MetOSitei | Q96291 |
Proteomic databases
PaxDbi | Q96291 |
PRIDEi | Q96291 |
ProteomicsDBi | 241127 |
Genome annotation databases
EnsemblPlantsi | AT3G11630.1; AT3G11630.1; AT3G11630 |
GeneIDi | 820335 |
Gramenei | AT3G11630.1; AT3G11630.1; AT3G11630 |
KEGGi | ath:AT3G11630 |
Organism-specific databases
Araporti | AT3G11630 |
TAIRi | locus:2080707, AT3G11630 |
Phylogenomic databases
eggNOGi | KOG0852, Eukaryota |
HOGENOMi | CLU_042529_21_0_1 |
InParanoidi | Q96291 |
OMAi | WWPKDFT |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q96291 |
Enzyme and pathway databases
BioCyci | ARA:AT3G11630-MONOMER |
BRENDAi | 1.11.1.15, 399 |
Miscellaneous databases
PROi | PR:Q96291 |
Gene expression databases
ExpressionAtlasi | Q96291, baseline and differential |
Genevisiblei | Q96291, AT |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAS1A_ARATH | |
Accessioni | Q96291Primary (citable) accession number: Q96291 Secondary accession number(s): P92938, Q8L5U1, Q9S7Y0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 11, 2001 | |
Last modified: | February 10, 2021 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families