UniProtKB - Q96247 (AUX1_ARATH)
Protein
Auxin transporter protein 1
Gene
AUX1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Carrier protein involved in proton-driven auxin influx. Mediates the formation of auxin gradient from developing leaves (site of auxin biosynthesis) to tips by contributing to the loading of auxin in vascular tissues and facilitating acropetal (base to tip) auxin transport within inner tissues of the root apex, and basipetal (tip to base) auxin transport within outer tissues of the root apex. Unloads auxin from the mature phloem to deliver the hormone to the root meristem via the protophloem cell files. Coordinated subcellular localization of AUX1 is regulated by a brefeldin A-sensitive (BFA) vesicle trafficking process. Involved in lateral root formation, trichoblast polarization and root hair elongation. Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins such as benzyladenine (BA). Needed for ammonium-mediated root-growth inhibition. Confers sensitivity to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D, auxin analog), and to polar auxin transport inhibitors such as N-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA).21 Publications
Activity regulationi
Auxin uptake mediated by AUX1 is inhibited by chromosaponin-1 (CSI), 1-naphthoxyacetic acid (1-NOA) and 3-chloro-4-hydroxyphenylacetic acid (CHPAA).2 Publications
GO - Molecular functioni
- amino acid transmembrane transporter activity Source: TAIR
- auxin binding Source: TAIR
- auxin influx transmembrane transporter activity Source: TAIR
- symporter activity Source: UniProtKB-KW
GO - Biological processi
- auxin-activated signaling pathway Source: UniProtKB-KW
- auxin polar transport Source: TAIR
- establishment of planar polarity Source: TAIR
- lateral root formation Source: TAIR
- positive gravitropism Source: TAIR
- response to nematode Source: TAIR
- root cap development Source: TAIR
- root hair cell differentiation Source: TAIR
Keywordsi
Biological process | Amino-acid transport, Auxin signaling pathway, Symport, Transport |
Protein family/group databases
TCDBi | 2.A.18.1.1, the amino acid/auxin permease (aaap) family |
Names & Taxonomyi
Protein namesi | Recommended name: Auxin transporter protein 1Alternative name(s): Auxin influx carrier protein 1 Polar auxin transport inhibitor-resistant protein 1 |
Gene namesi | Name:AUX1 Synonyms:AUX, PIR1, WAV5 Ordered Locus Names:At2g38120 ORF Names:F16M14.5 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G38120 |
TAIRi | locus:2005496, AT2G38120 |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Multi-pass membrane protein 3 Publications
Note: In S2 columella cells, a dynamic cytoplasmic to membrane localization seems to occur during early stage of gravity signal transduction. In roots protophloem cells, asymmetric repartition in the upper plasma membrane.
Endosome
- endosome Source: TAIR
Golgi apparatus
- Golgi apparatus Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- cell surface Source: TAIR
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 59 | CytoplasmicSequence analysisAdd BLAST | 59 | |
Transmembranei | 60 – 77 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 78 – 79 | ExtracellularSequence analysis | 2 | |
Transmembranei | 80 – 100 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 101 – 135 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 136 – 156 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 157 – 172 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 173 – 193 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 194 – 196 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 197 – 217 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 218 – 232 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 233 – 253 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 254 – 266 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 267 – 287 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 288 – 314 | ExtracellularSequence analysisAdd BLAST | 27 | |
Transmembranei | 315 – 335 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 336 – 356 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 357 – 377 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 378 | ExtracellularSequence analysis | 1 | |
Transmembranei | 379 – 399 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 400 – 425 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 426 – 446 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 447 – 485 | ExtracellularSequence analysisAdd BLAST | 39 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 57 | S → F in aux1-120; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 79 | G → R in aux1-110; resistance to 2,4-D. 1 Publication | 1 | |
Mutagenesisi | 178 | G → D in aux1-112; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 215 | S → F in aux1-111; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 238 | G → E in aux1-104; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 247 | G → E in aux1-106; resistance to 2,4-D, agravitropism. 1 Publication | 1 | |
Mutagenesisi | 250 | A → T in aux1-113; partial agravitropism. 1 Publication | 1 | |
Mutagenesisi | 259 | M → I in aux1-114; partial agravitropism. 1 Publication | 1 | |
Mutagenesisi | 262 | P → S in aux1-116; partial agravitropism. 1 Publication | 1 | |
Mutagenesisi | 272 | A → V in aux1-2; resistance to 2,4-D, lower growth rate of roots and hypocotyls, partial gravitropism. 1 Publication | 1 | |
Mutagenesisi | 292 | G → E in aux1-117; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 305 | M → R in aux1-102; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 325 | T → M in aux1-103; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 386 | S → N in aux1-118; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 439 | G → R in aux1-105; agravitropism. 1 Publication | 1 | |
Mutagenesisi | 459 | G → D in aux1-7; agravitropism, resistance to ethylene, auxin and 2,4-D. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093841 | 1 – 485 | Auxin transporter protein 1Add BLAST | 485 |
Proteomic databases
PaxDbi | Q96247 |
PRIDEi | Q96247 |
ProteomicsDBi | 240930 |
Expressioni
Tissue specificityi
Expressed in root and shoot apical tissues. In root apex, confined to stele initials, protophloem poles, statolith-containing S2 columella cells, lateral root cap cells (LRC), and in epidermal cells from the distal elongation zone (DEZ) up to central elongation zone (CEZ).3 Publications
Developmental stagei
In seedlings, first expressed in primary root, followed by shoot apical meristem and then in lateral roots. During lateral root formation, expression in primordia starts at a late phase of stage I, before the first periclinal division. In later stages II and III, localized in the apical tip, extending to the elongation zone, and in the vascular cylinder extending to the junction with the primary root.1 Publication
Gene expression databases
ExpressionAtlasi | Q96247, baseline and differential |
Genevisiblei | Q96247, AT |
Interactioni
Binary interactionsi
Q96247
With | #Exp. | IntAct |
---|---|---|
AXR4 [Q9FZ33] | 5 | EBI-16935343,EBI-25514394 |
Protein-protein interaction databases
BioGRIDi | 3734, 39 interactors |
IntActi | Q96247, 36 interactors |
STRINGi | 3702.AT2G38120.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1303, Eukaryota |
HOGENOMi | CLU_027994_2_0_1 |
InParanoidi | Q96247 |
OMAi | SACARQN |
OrthoDBi | 509343at2759 |
PhylomeDBi | Q96247 |
Family and domain databases
InterProi | View protein in InterPro IPR013057, AA_transpt_TM |
Pfami | View protein in Pfam PF01490, Aa_trans, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q96247-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEGVEAIVA NDNGTDQVNG NRTGKDNEEH DGSTGSNLSN FLWHGGSVWD
60 70 80 90 100
AWFSCASNQV AQVLLTLPYS FSQLGMLSGI VLQIFYGLLG SWTAYLISVL
110 120 130 140 150
YVEYRARKEK EGKSFKNHVI QWFEVLDGLL GSYWKALGLA FNCTFLLFGS
160 170 180 190 200
VIQLIACASN IYYINDHLDK RTWTYIFGAC CATTVFIPSF HNYRIWSFLG
210 220 230 240 250
LGMTTYTAWY LAIASIIHGQ AEGVKHSGPT KLVLYFTGAT NILYTFGGHA
260 270 280 290 300
VTVEIMHAMW KPQKFKYIYL MATLYVFTLT IPSAAAVYWA FGDALLDHSN
310 320 330 340 350
AFSLMPKNAW RDAAVILMLI HQFITFGFAC TPLYFVWEKV IGMHDTKSIC
360 370 380 390 400
LRALARLPVV IPIWFLAIIF PFFGPINSAV GALLVSFTVY IIPSLAHMLT
410 420 430 440 450
YRSASARQNA AEKPPFFMPS WTAMYVLNAF VVVWVLIVGF GFGGWASVTN
460 470 480
FVRQVDTFGL FAKCYQCKPA AAAAHAPVSA LHHRL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8B1Q8 | A0A1P8B1Q8_ARATH | Transmembrane amino acid transporte... | AUX1 AtAUX1, AUXIN RESISTANT 1, MAP1, MODIFIER OF ARF7, NPH4 PHENOTYPES 1 | 409 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X98772 Genomic DNA Translation: CAA67308.1 AC003028 Genomic DNA Translation: AAC27161.1 CP002685 Genomic DNA Translation: AEC09491.1 AY054488 mRNA Translation: AAK96679.1 AY093300 mRNA Translation: AAM13299.1 AY087092 mRNA Translation: AAM64652.1 |
PIRi | T01244 |
RefSeqi | NP_565882.1, NM_129368.3 |
Genome annotation databases
EnsemblPlantsi | AT2G38120.1; AT2G38120.1; AT2G38120 |
GeneIDi | 818390 |
Gramenei | AT2G38120.1; AT2G38120.1; AT2G38120 |
KEGGi | ath:AT2G38120 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X98772 Genomic DNA Translation: CAA67308.1 AC003028 Genomic DNA Translation: AAC27161.1 CP002685 Genomic DNA Translation: AEC09491.1 AY054488 mRNA Translation: AAK96679.1 AY093300 mRNA Translation: AAM13299.1 AY087092 mRNA Translation: AAM64652.1 |
PIRi | T01244 |
RefSeqi | NP_565882.1, NM_129368.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 3734, 39 interactors |
IntActi | Q96247, 36 interactors |
STRINGi | 3702.AT2G38120.1 |
Protein family/group databases
TCDBi | 2.A.18.1.1, the amino acid/auxin permease (aaap) family |
Proteomic databases
PaxDbi | Q96247 |
PRIDEi | Q96247 |
ProteomicsDBi | 240930 |
Genome annotation databases
EnsemblPlantsi | AT2G38120.1; AT2G38120.1; AT2G38120 |
GeneIDi | 818390 |
Gramenei | AT2G38120.1; AT2G38120.1; AT2G38120 |
KEGGi | ath:AT2G38120 |
Organism-specific databases
Araporti | AT2G38120 |
TAIRi | locus:2005496, AT2G38120 |
Phylogenomic databases
eggNOGi | KOG1303, Eukaryota |
HOGENOMi | CLU_027994_2_0_1 |
InParanoidi | Q96247 |
OMAi | SACARQN |
OrthoDBi | 509343at2759 |
PhylomeDBi | Q96247 |
Miscellaneous databases
PROi | PR:Q96247 |
Gene expression databases
ExpressionAtlasi | Q96247, baseline and differential |
Genevisiblei | Q96247, AT |
Family and domain databases
InterProi | View protein in InterPro IPR013057, AA_transpt_TM |
Pfami | View protein in Pfam PF01490, Aa_trans, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AUX1_ARATH | |
Accessioni | Q96247Primary (citable) accession number: Q96247 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families