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Protein

5'-AMP-activated protein kinase catalytic subunit alpha-2

Gene

aak-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals (PubMed:15574588). Role in motility and response to oxidative stress (PubMed:18408008, PubMed:24763318). Involved in the establishment of germline stem cell (GSC) quiescence during dauer development (PubMed:15574588, PubMed:20110331). Plays a role in axon regrowth after axotomy in PLM neurons (PubMed:24431434). Plays a role in the maintenance of glycogen stores which are necessary for resistance to hyperosmotic stress (PubMed:26439621). Plays a role in the regulation of flp-7 secretion from ASI neurons (PubMed:28128367). Keeps the CREB-regulated transcription coactivator 1 homolog crtc-1 inactive which in turn inhibits flp-7 secretion (PubMed:28128367). Following serotonin signaling, derepresses crtc-1 which stimulates flp-7 secretion and subsequent body fat loss (PubMed:28128367).7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by phosphorylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116ATPPROSITE-ProRule annotation1
Active sitei210Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi93 – 101ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • AMP-activated protein kinase activity Source: WormBase
  • ATP binding Source: UniProtKB-KW
  • histone serine kinase activity Source: GO_Central

GO - Biological processi

  • cellular response to glucose starvation Source: GO_Central
  • determination of adult lifespan Source: WormBase
  • intracellular signal transduction Source: GO_Central
  • negative regulation of protein secretion Source: UniProtKB
  • negative regulation of TOR signaling Source: GO_Central
  • peptidyl-serine phosphorylation Source: WormBase
  • positive regulation of dauer larval development Source: WormBase
  • positive regulation of protein secretion Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1632852 Macroautophagy
R-CEL-163680 AMPK inhibits chREBP transcriptional activation activity
R-CEL-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-CEL-5628897 TP53 Regulates Metabolic Genes
SignaLinkiQ95ZQ4

Names & Taxonomyi

Protein namesi
Recommended name:
5'-AMP-activated protein kinase catalytic subunit alpha-2 (EC:2.7.11.1)
Short name:
AMPK subunit alpha-2
Gene namesi
Name:aak-2
ORF Names:T01C8.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiT01C8.1a ; CE31222 ; WBGene00020142 ; aak-2
T01C8.1b ; CE31223 ; WBGene00020142 ; aak-2
T01C8.1c ; CE07458 ; WBGene00020142 ; aak-2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Shortened life-span (PubMed:15574588, PubMed:18408008). Temperature-dependent hypersensitivity to stress, slow body bending, abnormal modulation of head oscillation (PubMed:15574588, PubMed:18408008). Reduced survival following oxidative stress induced by the superoxide paraquat (PubMed:24763318). Reduced survival as a result of hyperosmotic stress induced by NaCl (PubMed:26439621). Double knockout with aak-1 results in reduced glycogen accumulation (PubMed:26439621).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000855991 – 6265'-AMP-activated protein kinase catalytic subunit alpha-2Add BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei243Phosphothreonine; by par-42 Publications1

Post-translational modificationi

Phosphorylated on Thr-243 in response to oxidative stress and during dauer development.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ95ZQ4
PaxDbiQ95ZQ4
PeptideAtlasiQ95ZQ4
PRIDEiQ95ZQ4

PTM databases

iPTMnetiQ95ZQ4

Expressioni

Tissue specificityi

Expressed in the pharynx, the ventral cord, neurons including the hermaphrodite-specific neuron, body wall muscles, the vulva, the excretory canal, and weakly in the intestine.1 Publication

Gene expression databases

BgeeiWBGene00020142

Interactioni

Subunit structurei

Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer that binds four cAMP molecules.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
aakb-2Q9NAH74EBI-326514,EBI-2417663

Protein-protein interaction databases

BioGridi46610, 8 interactors
DIPiDIP-25170N
IntActiQ95ZQ4, 10 interactors
STRINGi6239.T01C8.1b

Structurei

3D structure databases

ProteinModelPortaliQ95ZQ4
SMRiQ95ZQ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 339Protein kinasePROSITE-ProRule annotationAdd BLAST253

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0586 Eukaryota
ENOG410XNQ0 LUCA
GeneTreeiENSGT00900000140868
HOGENOMiHOG000233016
InParanoidiQ95ZQ4
KOiK07198
OMAiRWHFGIR
OrthoDBiEOG091G03TG
PhylomeDBiQ95ZQ4

Family and domain databases

InterProiView protein in InterPro
IPR032270 AMPK_C
IPR028375 KA1/Ssp2_C
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF16579 AdenylateSensor, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF103243 SSF103243, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b1 Publication (identifier: Q95ZQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFSHQDRDRD RKEDGGGDGT EMKSKSRSQP SGLNRVKNLS RKLSAKSRKE
60 70 80 90 100
RKDRDSTDNS SKMSSPGGET STKQQQELKA QIKIGHYILK ETLGVGTFGK
110 120 130 140 150
VKVGIHETTQ YKVAVKILNR QKIKSLDVVG KIRREIQNLS LFRHPHIIRL
160 170 180 190 200
YQVISTPSDI FMIMEHVSGG ELFDYIVKHG RLKTAEARRF FQQIISGVDY
210 220 230 240 250
CHRHMVVHRD LKPENLLLDE QNNVKIADFG LSNIMTDGDF LRTSCGSPNY
260 270 280 290 300
AAPEVISGKL YAGPEVDVWS CGVILYALLC GTLPFDDEHV PSLFRKIKSG
310 320 330 340 350
VFPTPDFLER PIVNLLHHML CVDPMKRATI KDVIAHEWFQ KDLPNYLFPP
360 370 380 390 400
INESEASIVD IEAVREVTEF QRYHVAEEEV TSALLGDDPH HHLSIAYNLI
410 420 430 440 450
VDNKRIADET AKLSIEEFYQ VTPNKGPGPV HRHPERIAAS VSSKITPTLD
460 470 480 490 500
NTEASGANRN KRAKWHLGIR SQSRPEDIMF EVFRAMKQLD MEWKVLNPYH
510 520 530 540 550
VIVRRKPDAP AADPPKMSLQ LYQVDQRSYL LDFKSLADEE SGSASASSSR
560 570 580 590 600
HASMSMPQKP AGIRGTRTSS MPQAMSMEAS IEKMEVHDFS DMSCDVTPPP
610 620
SPGGAKLSQT MQFFEICAAL IGTLAR
Note: No experimental confirmation available.Curated
Length:626
Mass (Da):70,443
Last modified:October 1, 2002 - v2
Checksum:iD1401DB1286D8624
GO
Isoform a1 Publication (identifier: Q95ZQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: Missing.

Show »
Length:624
Mass (Da):70,168
Checksum:i3BF9F9610BA95DB7
GO
Isoform c1 Publication (identifier: Q95ZQ4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.
     370-371: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:562
Mass (Da):63,103
Checksum:iAA34A96C5BBCAFBD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0519401 – 62Missing in isoform c. 1 PublicationAdd BLAST62
Alternative sequenceiVSP_051941370 – 371Missing in isoform a and isoform c. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY347273 mRNA Translation: AAR06928.1
FO081053 Genomic DNA Translation: CCD68833.1
FO081053 Genomic DNA Translation: CCD68834.1
FO081053 Genomic DNA Translation: CCD68835.1
PIRiT29858
RefSeqiNP_001024868.1, NM_001029697.3 [Q95ZQ4-3]
NP_510710.2, NM_078309.4 [Q95ZQ4-1]
NP_510711.2, NM_078310.5 [Q95ZQ4-2]
UniGeneiCel.17479

Genome annotation databases

EnsemblMetazoaiT01C8.1b; T01C8.1b; WBGene00020142 [Q95ZQ4-1]
GeneIDi181727
KEGGicel:CELE_T01C8.1
UCSCiT01C8.1c.1 c. elegans [Q95ZQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAAPK2_CAEEL
AccessioniPrimary (citable) accession number: Q95ZQ4
Secondary accession number(s): Q22068, Q86FL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2002
Last modified: July 18, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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