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Entry version 102 (29 Sep 2021)
Sequence version 1 (01 Dec 2001)
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Protein

Protein CARMIL

Gene

carmil

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Serves as the scaffold for the assembly of a complex that links key players in the nucleation and termination of actin filament assembly with a ubiquitous barbed end-directed motor. This complex is composed of at least capping proteins (acpA and acpB), the Arp2/3 complex, type I myosins (myoB and myoC) and carmil. It has at least a modest ability to activate Arp2/3-dependent actin nucleation. CARMIL localizes along with the Arp2/3 complex, myoB, and myoC in the leading edge of cells and it plays a significant role in the structure and function of these actin-rich cellular extensions.

1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • myosin I binding Source: dictyBase

GO - Biological processi

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-DDI-983231, Factors involved in megakaryocyte development and platelet production

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Protein CARMIL
Short name:
dDcarmil
Alternative name(s):
Capping protein, arp2/3 and myosin I linker
Leucine-rich repeat-containing protein p116
Short name:
p116
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:carmil
ORF Names:DDB_G0292386
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDictyostelium discoideum (Slime mold)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri44689 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaAmoebozoaEvoseaEumycetozoaDictyosteliaDictyostelialesDictyosteliaceaeDictyostelium
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002195 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

Dictyostelium discoideum online informatics resource

More...
dictyBasei
DDB_G0292386, carmil

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywords - Cellular componenti

Cell projection

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells exhibit a striking defect in the formation of dorsal, cup-like, macropinocytic structures, a concomitant reduction in the rate of fluid phase pinocytosis, a significant decrease in the efficiency of chemotactic aggregation, and a decrease in cellular F-actin content.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003904021 – 1050Protein CARMILAdd BLAST1050

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q95VZ3

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Down-regulated by Pseudomonas aeruginosa, PAO1 strain and PA14 strain infection.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts (via PXXP domains) with myoB and myoC (via SH3 domain).

Interacts (via N-terminus) with the capping proteins acpA and acpB.

Interacts (via the region between the LRR domain and COOH-terminal proline-rich domain) with the seven member Arp2/3 complex (arpB/Arp2, arpC/Arp3, arcA/p41-arc, arcB/p34-arc, arcC/p21-arc, arcD/p20-arc and arcE/p16-arc).

1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
44689.DDB0185176

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q95VZ3

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati243 – 266LRR 1Add BLAST24
Repeati268 – 291LRR 2Add BLAST24
Repeati329 – 352LRR 3Add BLAST24
Repeati383 – 409LRR 4Add BLAST27
Repeati411 – 436LRR 5Add BLAST26
Repeati442 – 464LRR 6Add BLAST23
Repeati470 – 493LRR 7Add BLAST24
Repeati563 – 590LRR 8Add BLAST28

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 179Required for interaction with capping proteinAdd BLAST179
Regioni851 – 931DisorderedSequence analysisAdd BLAST81
Regioni945 – 1050DisorderedSequence analysisAdd BLAST106

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi959 – 963PXXP motif; important for binding to SH3 domain-containing proteins5
Motifi968 – 971PXXP motif; important for binding to SH3 domain-containing proteins4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi864 – 889Pro residuesSequence analysisAdd BLAST26
Compositional biasi901 – 923Pro residuesSequence analysisAdd BLAST23
Compositional biasi957 – 971Pro residuesSequence analysisAdd BLAST15
Compositional biasi985 – 1001Polar residuesSequence analysisAdd BLAST17
Compositional biasi1011 – 1025Polar residuesSequence analysisAdd BLAST15

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the CARMIL family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG4242, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_003119_3_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q95VZ3

Identification of Orthologs from Complete Genome Data

More...
OMAi
FRDEVVW

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q95VZ3

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.30.29.30, 1 hit
3.80.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR041245, CARMIL_PH
IPR001611, Leu-rich_rpt
IPR032675, LRR_dom_sf
IPR011993, PH-like_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF17888, Carm_PH, 1 hit
PF13516, LRR_6, 3 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q95VZ3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEEISPNDR KFITNLLTQK NQESLLLSIG DKISKKKNKK PSKRIILITK
60 70 80 90 100
NRIFFLKPSQ NKVKKDIHLL DIQEIKSSTS NEFTIVAKVD NKQFSYGLIT
110 120 130 140 150
NKTDEIINQI RVTFNHQFFG CPEESTFKCT DIKDSRLVEI EQKDLPCGGF
160 170 180 190 200
VETYQSICDH LGVPPRDDIC WDMTNIISSK NIRSFNIGEI ELPTSAGDTI
210 220 230 240 250
RCLLGALKYN NYFKSFNFNN YTFNKEQFGY LAEVLKCNST VEDLSLNNVG
260 270 280 290 300
LKHDTMPIIA TALSSNKNLA LTAIDISNNQ IEDKGMTAFS SYVASSLRGI
310 320 330 340 350
ASLDVSNTNC NKAGISVLTN ALKKNIKMSS TLSYLNLSGN KMEADGSAGL
360 370 380 390 400
SSFLASPNTL KTLNISNTTP SMETIVGALV IGCAELKTID ISDNKLTKKE
410 420 430 440 450
VPHLVRFIGA SSTLKHFNLS GTKVPVENLK ELVVAITSNI YLQDVVLDLK
460 470 480 490 500
NNDLGIAGAR MLASLATDKL SNVIYLDVSE NDFGDEGVSV ICDGFVGNST
510 520 530 540 550
IKKLILNGNF KQSKTKSRPS AIESVISLLE SECPLETLHM TVGNSKSPLK
560 570 580 590 600
ADILSLIYSL ATNSSLLELD ISGHQMGPKG AIGLGKALQT NKTLHTLIWD
610 620 630 640 650
DNLTTAIGFA GFQVGLERNL TLKNMPTPLN DIIQCHREPK FQQIWKEIDS
660 670 680 690 700
CINRNQSPTR AFEGNGGNSI GATNLSFLAS GQQQGVEKLL NKIKSIGRKV
710 720 730 740 750
TDPNNILIVK DAESTEKVIG GIHLIKESIH ASLEMELNQK LKDFVQVVND
760 770 780 790 800
VINAKKNEMT QQILESMQNT FQSMDGPTIK RLATTIQYGS KDVDEQQIHS
810 820 830 840 850
TLVKGAGAEL SSRAHECFIS ALDIASDYTY EKITIGLDSV FKDLILEESQ
860 870 880 890 900
AQNEASGATP IPDSPVPTRS PQPTSPPITP QPTPTTNVPP VTAPRTGAAA
910 920 930 940 950
PLKPANPPPV STTTTPPVST TPKPTQPVSK FGAKLSANSA VAEAIARNMG
960 970 980 990 1000
GGAPPIRKPV APEPEPEPVT PTKDVTPLKS KPVVAPRSTP TTSTPTKTPV
1010 1020 1030 1040 1050
KKPSGPSVPG SLSDAPESDS AELTHVTASR PHIASKRKPP TRRPRPPTEN
Length:1,050
Mass (Da):113,887
Last modified:December 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD6F18849AA9C692B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti852Q → G AA sequence (PubMed:11425877).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF388524 Genomic DNA Translation: AAK72255.1
AAFI02000190 Genomic DNA Translation: EAL61172.1

NCBI Reference Sequences

More...
RefSeqi
XP_629656.1, XM_629654.1

Genome annotation databases

Ensembl protists genome annotation project

More...
EnsemblProtistsi
EAL61172; EAL61172; DDB_G0292386

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
8628720

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ddi:DDB_G0292386

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF388524 Genomic DNA Translation: AAK72255.1
AAFI02000190 Genomic DNA Translation: EAL61172.1
RefSeqiXP_629656.1, XM_629654.1

3D structure databases

SMRiQ95VZ3
ModBaseiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0185176

Proteomic databases

PaxDbiQ95VZ3

Genome annotation databases

EnsemblProtistsiEAL61172; EAL61172; DDB_G0292386
GeneIDi8628720
KEGGiddi:DDB_G0292386

Organism-specific databases

dictyBaseiDDB_G0292386, carmil

Phylogenomic databases

eggNOGiKOG4242, Eukaryota
HOGENOMiCLU_003119_3_0_1
InParanoidiQ95VZ3
OMAiFRDEVVW
PhylomeDBiQ95VZ3

Enzyme and pathway databases

ReactomeiR-DDI-983231, Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q95VZ3

Family and domain databases

Gene3Di2.30.29.30, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR041245, CARMIL_PH
IPR001611, Leu-rich_rpt
IPR032675, LRR_dom_sf
IPR011993, PH-like_dom_sf
PfamiView protein in Pfam
PF17888, Carm_PH, 1 hit
PF13516, LRR_6, 3 hits

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCARML_DICDI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q95VZ3
Secondary accession number(s): Q54D40
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 1, 2001
Last modified: September 29, 2021
This is version 102 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families
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