Endonuclease G, mitochondrial

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactorⁱ

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• endodeoxyribonuclease activity Source: WormBase <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Mechanisms of AIF-mediated apoptotic DNA degradation in Caenorhabditis elegans."
    Wang X., Yang C., Chai J., Shi Y., Xue D.
    Science 298:1587-1592(2002) [PubMed] [Europe PMC] [Abstract]
  • "CRN-1, a Caenorhabditis elegans FEN-1 homologue, cooperates with CPS-6/EndoG to promote apoptotic DNA degradation."
    Parrish J.Z., Yang C., Shen B., Xue D.
    EMBO J. 22:3451-3460(2003) [PubMed] [Europe PMC] [Abstract]
  • "Production and characterization of recombinant protein preparations of Endonuclease G-homologs from yeast, C. elegans and humans."
    Kieper J., Lauber C., Gimadutdinow O., Urbanska A., Cymerman I., Ghosh M., Szczesny B., Meiss G.
    Protein Expr. Purif. 73:99-106(2010) [PubMed] [Europe PMC] [Abstract]
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]
• endonuclease activity Source: WormBaseInferred from direct assayⁱ
  • Ref.1

    "Mitochondrial endonuclease G is important for apoptosis in C. elegans."
    Parrish J., Li L., Klotz K., Ledwich D., Wang X., Xue D.
    Nature 412:90-94(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
• endoribonuclease activity Source: WormBaseInferred from direct assayⁱ
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]
• metal ion binding Source: UniProtKB-KW
• protein homodimerization activity Source: WormBase <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]
• sequence-specific DNA binding Source: WormBaseInferred from direct assayⁱ
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]
• single-stranded DNA endodeoxyribonuclease activity Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• apoptotic DNA fragmentation Source: WormBaseInferred from direct assayⁱ
  • Ref.1

    "Mitochondrial endonuclease G is important for apoptosis in C. elegans."
    Parrish J., Li L., Klotz K., Ledwich D., Wang X., Xue D.
    Nature 412:90-94(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
• apoptotic process Source: WormBase <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • Ref.1

    "Mitochondrial endonuclease G is important for apoptosis in C. elegans."
    Parrish J., Li L., Klotz K., Ledwich D., Wang X., Xue D.
    Nature 412:90-94(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]
• DNA catabolic process, endonucleolytic Source: WormBaseInferred from direct assayⁱ
  • "Mechanisms of AIF-mediated apoptotic DNA degradation in Caenorhabditis elegans."
    Wang X., Yang C., Chai J., Shi Y., Xue D.
    Science 298:1587-1592(2002) [PubMed] [Europe PMC] [Abstract]
  • "CRN-1, a Caenorhabditis elegans FEN-1 homologue, cooperates with CPS-6/EndoG to promote apoptotic DNA degradation."
    Parrish J.Z., Yang C., Shen B., Xue D.
    EMBO J. 22:3451-3460(2003) [PubMed] [Europe PMC] [Abstract]
  • "Production and characterization of recombinant protein preparations of Endonuclease G-homologs from yeast, C. elegans and humans."
    Kieper J., Lauber C., Gimadutdinow O., Urbanska A., Cymerman I., Ghosh M., Szczesny B., Meiss G.
    Protein Expr. Purif. 73:99-106(2010) [PubMed] [Europe PMC] [Abstract]
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]
• RNA catabolic process Source: WormBaseInferred from direct assayⁱ
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

GO - Molecular functionⁱ

• protein homodimerization activity Source: WormBaseInferred from physical interactionⁱ
  • "Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans."
    Lin J.L., Nakagawa A., Lin C.L., Hsiao Y.Y., Yang W.Z., Wang Y.T., Doudeva L.G., Skeen-Gaar R.R., Xue D., Yuan H.S.
    J. Biol. Chem. 287:7110-7120(2012) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MIGKVAGTAA IAGISFLAGK YSNDDLPIFR NVQSATNVPM NQIQVSEPMT 
        60         70         80         90        100
VKPASLNADA MGPSRSAEIM KHGYPGFTNV RTYEDFVLSY DYKTRTAHWV 
       110        120        130        140        150
CEHLTPERLK HAEGVDRKLC EFKPDITFPQ KFLSQNTDYK CSGFDRGHLA 
       160        170        180        190        200
AAGNHRKSQL AVDQTFYLSN MSPQVGRGFN RDKWNDLEMH CRRVAKKMIN 
       210        220        230        240        250
SYIITGPLYL PKLEGDGKKY IKYQVIGDNN VAVPTHFFKV ALFEVTPGKF 
       260        270        280        290        300
ELESYILPNA VIEDTVEISK FHVPLDAVER SAGLEIFARL DPKSIVKENG 

AKKGGLLW                                               

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. SIMILARITY comments
   Index of protein domains and families
2. Caenorhabditis elegans
   Caenorhabditis elegans: entries, gene names and cross-references to WormBase
3. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references