UniProtKB - Q95NM6 (NUCG_CAEEL)
Protein
Endonuclease G, mitochondrial
Gene
cps-6
Organism
Caenorhabditis elegans
Status
Functioni
Endonuclease important for programmed cell death; it mediates apoptotic DNA fragmentation.1 Publication
Miscellaneous
The active site contains 1 hydrated magnesium ion that has only 1 direct interaction with the protein; all other interactions are via water molecules.By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 148 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 180 | Magnesium; catalyticBy similarity | 1 |
GO - Molecular functioni
- endodeoxyribonuclease activity Source: WormBase
- endonuclease activity Source: WormBase
- endoribonuclease activity Source: WormBase
- metal ion binding Source: UniProtKB-KW
- protein homodimerization activity Source: WormBase
- sequence-specific DNA binding Source: WormBase
- single-stranded DNA endodeoxyribonuclease activity Source: GO_Central
GO - Biological processi
- apoptotic DNA fragmentation Source: WormBase
- DNA catabolic process, endonucleolytic Source: WormBase
- RNA catabolic process Source: WormBase
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | Apoptosis |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Endonuclease G, mitochondrial (EC:3.1.30.-)Short name: Endo G Alternative name(s): Ced-3 protease suppressor 6 |
Gene namesi | Name:cps-6 ORF Names:C41D11.8 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C41D11.8 ; CE29488 ; WBGene00000787 ; cps-6 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrial inner membrane Source: GO_Central
- mitochondrion Source: WormBase
Nucleus
- nucleus Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000019920 | ? – 308 | Endonuclease G, mitochondrial |
Proteomic databases
EPDi | Q95NM6 |
PaxDbi | Q95NM6 |
PeptideAtlasi | Q95NM6 |
Expressioni
Gene expression databases
Bgeei | WBGene00000787, Expressed in material anatomical entity and 5 other tissues |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: WormBase
Protein-protein interaction databases
BioGRIDi | 37514, 9 interactors |
IntActi | Q95NM6, 2 interactors |
STRINGi | 6239.C41D11.8 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q95NM6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the DNA/RNA non-specific endonuclease family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3721, Eukaryota |
GeneTreei | ENSGT00940000160987 |
HOGENOMi | CLU_055174_0_1_1 |
InParanoidi | Q95NM6 |
OMAi | YVMPNQV |
OrthoDBi | 933605at2759 |
PhylomeDBi | Q95NM6 |
Family and domain databases
InterProi | View protein in InterPro IPR018524, DNA/RNA_endonuclease_AS IPR001604, DNA/RNA_non-sp_Endonuclease IPR020821, Extracellular_endonuc_su_A IPR040255, Non-specific_endonuclease |
PANTHERi | PTHR13966, PTHR13966, 1 hit |
Pfami | View protein in Pfam PF01223, Endonuclease_NS, 1 hit |
SMARTi | View protein in SMART SM00892, Endonuclease_NS, 1 hit SM00477, NUC, 1 hit |
PROSITEi | View protein in PROSITE PS01070, NUCLEASE_NON_SPEC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q95NM6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIGKVAGTAA IAGISFLAGK YSNDDLPIFR NVQSATNVPM NQIQVSEPMT
60 70 80 90 100
VKPASLNADA MGPSRSAEIM KHGYPGFTNV RTYEDFVLSY DYKTRTAHWV
110 120 130 140 150
CEHLTPERLK HAEGVDRKLC EFKPDITFPQ KFLSQNTDYK CSGFDRGHLA
160 170 180 190 200
AAGNHRKSQL AVDQTFYLSN MSPQVGRGFN RDKWNDLEMH CRRVAKKMIN
210 220 230 240 250
SYIITGPLYL PKLEGDGKKY IKYQVIGDNN VAVPTHFFKV ALFEVTPGKF
260 270 280 290 300
ELESYILPNA VIEDTVEISK FHVPLDAVER SAGLEIFARL DPKSIVKENG
AKKGGLLW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF390558 mRNA Translation: AAK72007.1 FO080509 Genomic DNA Translation: CCD64257.1 |
RefSeqi | NP_491371.1, NM_058970.4 |
Genome annotation databases
EnsemblMetazoai | C41D11.8.1; C41D11.8.1; WBGene00000787 |
GeneIDi | 172045 |
KEGGi | cel:CELE_C41D11.8 |
UCSCi | C41D11.8.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF390558 mRNA Translation: AAK72007.1 FO080509 Genomic DNA Translation: CCD64257.1 |
RefSeqi | NP_491371.1, NM_058970.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3S5B | X-ray | 1.80 | A/B | 63-303 | [»] | |
4QN0 | X-ray | 2.74 | A/B/C/D | 64-302 | [»] | |
5GKC | X-ray | 1.89 | A/B | 63-305 | [»] | |
5GKP | X-ray | 2.30 | A/B | 63-305 | [»] | |
SMRi | Q95NM6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 37514, 9 interactors |
IntActi | Q95NM6, 2 interactors |
STRINGi | 6239.C41D11.8 |
Proteomic databases
EPDi | Q95NM6 |
PaxDbi | Q95NM6 |
PeptideAtlasi | Q95NM6 |
Genome annotation databases
EnsemblMetazoai | C41D11.8.1; C41D11.8.1; WBGene00000787 |
GeneIDi | 172045 |
KEGGi | cel:CELE_C41D11.8 |
UCSCi | C41D11.8.1, c. elegans |
Organism-specific databases
CTDi | 172045 |
WormBasei | C41D11.8 ; CE29488 ; WBGene00000787 ; cps-6 |
Phylogenomic databases
eggNOGi | KOG3721, Eukaryota |
GeneTreei | ENSGT00940000160987 |
HOGENOMi | CLU_055174_0_1_1 |
InParanoidi | Q95NM6 |
OMAi | YVMPNQV |
OrthoDBi | 933605at2759 |
PhylomeDBi | Q95NM6 |
Miscellaneous databases
PROi | PR:Q95NM6 |
Gene expression databases
Bgeei | WBGene00000787, Expressed in material anatomical entity and 5 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR018524, DNA/RNA_endonuclease_AS IPR001604, DNA/RNA_non-sp_Endonuclease IPR020821, Extracellular_endonuc_su_A IPR040255, Non-specific_endonuclease |
PANTHERi | PTHR13966, PTHR13966, 1 hit |
Pfami | View protein in Pfam PF01223, Endonuclease_NS, 1 hit |
SMARTi | View protein in SMART SM00892, Endonuclease_NS, 1 hit SM00477, NUC, 1 hit |
PROSITEi | View protein in PROSITE PS01070, NUCLEASE_NON_SPEC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUCG_CAEEL | |
Accessioni | Q95NM6Primary (citable) accession number: Q95NM6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2002 |
Last sequence update: | December 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families