UniProtKB - Q95M17 (CHIA_BOVIN)
Protein
Acidic mammalian chitinase
Gene
CHIA
Organism
Bos taurus (Bovine)
Status
Functioni
Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding (By similarity).By similarity1 Publication
Catalytic activityi
- Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. EC:3.2.1.14
pH dependencei
Optimum pH is 5.6-7.6.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 140 | Proton donorPROSITE-ProRule annotation | 1 | |
Binding sitei | 141 | ChitooligosaccharideBy similarity | 1 | |
Binding sitei | 360 | ChitooligosaccharideBy similarity | 1 |
GO - Molecular functioni
- chitinase activity Source: UniProtKB
- chitin binding Source: GO_Central
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- chitin catabolic process Source: UniProtKB
- immune system process Source: UniProtKB-KW
- polysaccharide catabolic process Source: UniProtKB-KW
- positive regulation of chemokine secretion Source: UniProtKB
- production of molecular mediator involved in inflammatory response Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Apoptosis, Carbohydrate metabolism, Chitin degradation, Immunity, Inflammatory response, Polysaccharide degradation |
Ligand | Chitin-binding |
Protein family/group databases
CAZyi | CBM14 Carbohydrate-Binding Module Family 14 GH18 Glycoside Hydrolase Family 18 |
Names & Taxonomyi
Protein namesi | Recommended name: Acidic mammalian chitinase (EC:3.2.1.14)Short name: AMCase Alternative name(s): Chitin-binding protein b04 Short name: CBPb04 |
Gene namesi | Name:CHIA |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- extracellular region Source: GO_Central
- extracellular space Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | 1 PublicationAdd BLAST | 21 | |
ChainiPRO_0000011943 | 22 – 472 | Acidic mammalian chitinaseAdd BLAST | 451 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 26 ↔ 51 | PROSITE-ProRule annotation | ||
Disulfide bondi | 49 ↔ 394 | PROSITE-ProRule annotation | ||
Disulfide bondi | 307 ↔ 372 | PROSITE-ProRule annotation | ||
Glycosylationi | 395 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 409 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 415 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 456 ↔ 469 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q95M17 |
PRIDEi | Q95M17 |
Expressioni
Tissue specificityi
Detected in liver and in serum.1 Publication
Interactioni
Subunit structurei
Interacts with EGFR.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000000324 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 423 – 472 | Chitin-binding type-2PROSITE-ProRule annotationAdd BLAST | 50 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 70 – 71 | Chitooligosaccharide bindingBy similarity | 2 | |
Regioni | 97 – 100 | Chitooligosaccharide bindingBy similarity | 4 | |
Regioni | 210 – 213 | Chitooligosaccharide bindingBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 417 – 420 | Poly-Ser | 4 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2806 Eukaryota COG3325 LUCA |
HOGENOMi | HOG000111109 |
InParanoidi | Q95M17 |
KOi | K01183 |
OrthoDBi | 826687at2759 |
Family and domain databases
Gene3Di | 3.10.50.10, 1 hit |
InterProi | View protein in InterPro IPR002557 Chitin-bd_dom IPR036508 Chitin-bd_dom_sf IPR011583 Chitinase_II IPR029070 Chitinase_insertion_sf IPR001223 Glyco_hydro18_cat IPR001579 Glyco_hydro_18_chit_AS IPR017853 Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF01607 CBM_14, 1 hit PF00704 Glyco_hydro_18, 1 hit |
SMARTi | View protein in SMART SM00494 ChtBD2, 1 hit SM00636 Glyco_18, 1 hit |
SUPFAMi | SSF51445 SSF51445, 1 hit SSF54556 SSF54556, 1 hit SSF57625 SSF57625, 1 hit |
PROSITEi | View protein in PROSITE PS50940 CHIT_BIND_II, 1 hit PS01095 CHITINASE_18, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q95M17-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKLIFLTGL AFLLNAQLGS AYQLVCYFSN WAQYRPGLGS FKPDNIDPCL
60 70 80 90 100
CTHLIYAFAG MSNSEITTIE WNDVALYSSF NDLKKKNSQL KILLAIGGWN
110 120 130 140 150
FGTAPFTAMV ATPENRKTFI SSVIKFLHQY GFDGLDFDWE YPGFRGSPSQ
160 170 180 190 200
DKHLFTVLVQ ETREAFEQEA KQTNKPRLLV TAAVAAGISN IQAGYEIPQL
210 220 230 240 250
SQYLDFIHVM TYDFHGSWEG YTGENSPLYK YPTDTGSNTY LNVEYAMNYW
260 270 280 290 300
KKNGAPAEKL IIGFPAYGHN FILRDASNNG IGAPTSGAGP AGPYTREAGF
310 320 330 340 350
WAYYEICAFL KDGATEAWDD SQNVPYAYKG TEWVGYDNVN SFRIKAQWLK
360 370 380 390 400
ENNFGGAMVW AIDLDDFTGT FCNQGKFPLI NTLKDALGLK SATCNASTQS
410 420 430 440 450
SEPNSSPGNE SGSGNKSSSS EGRGYCAGKA DGLYPVADNR NAFWNCVNGI
460 470
TYKQNCLTGL VFDTSCHCCN WA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 144 | F → S in AAI02932 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB051629 mRNA Translation: BAB71805.1 BC102931 mRNA Translation: AAI02932.1 |
RefSeqi | NP_777124.1, NM_174699.2 |
Genome annotation databases
GeneIDi | 282645 |
KEGGi | bta:282645 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB051629 mRNA Translation: BAB71805.1 BC102931 mRNA Translation: AAI02932.1 |
RefSeqi | NP_777124.1, NM_174699.2 |
3D structure databases
SMRi | Q95M17 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000000324 |
Protein family/group databases
CAZyi | CBM14 Carbohydrate-Binding Module Family 14 GH18 Glycoside Hydrolase Family 18 |
Proteomic databases
PaxDbi | Q95M17 |
PRIDEi | Q95M17 |
Genome annotation databases
GeneIDi | 282645 |
KEGGi | bta:282645 |
Organism-specific databases
CTDi | 27159 |
Phylogenomic databases
eggNOGi | KOG2806 Eukaryota COG3325 LUCA |
HOGENOMi | HOG000111109 |
InParanoidi | Q95M17 |
KOi | K01183 |
OrthoDBi | 826687at2759 |
Family and domain databases
Gene3Di | 3.10.50.10, 1 hit |
InterProi | View protein in InterPro IPR002557 Chitin-bd_dom IPR036508 Chitin-bd_dom_sf IPR011583 Chitinase_II IPR029070 Chitinase_insertion_sf IPR001223 Glyco_hydro18_cat IPR001579 Glyco_hydro_18_chit_AS IPR017853 Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF01607 CBM_14, 1 hit PF00704 Glyco_hydro_18, 1 hit |
SMARTi | View protein in SMART SM00494 ChtBD2, 1 hit SM00636 Glyco_18, 1 hit |
SUPFAMi | SSF51445 SSF51445, 1 hit SSF54556 SSF54556, 1 hit SSF57625 SSF57625, 1 hit |
PROSITEi | View protein in PROSITE PS50940 CHIT_BIND_II, 1 hit PS01095 CHITINASE_18, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHIA_BOVIN | |
Accessioni | Q95M17Primary (citable) accession number: Q95M17 Secondary accession number(s): Q3SZE1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | September 18, 2019 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families