UniProtKB - Q95168 (ZO2_CANLF)
Protein
Tight junction protein ZO-2
Gene
TJP2
Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Functioni
Plays a role in tight junctions and adherens junctions.
GO - Molecular functioni
- cell adhesion molecule binding Source: GO_Central
GO - Biological processi
- cell-cell adhesion Source: GO_Central
- cell-cell junction organization Source: GO_Central
- establishment of endothelial intestinal barrier Source: GO_Central
- positive regulation of blood-brain barrier permeability Source: GO_Central
- protein localization to cell-cell junction Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Tight junction protein ZO-2Alternative name(s): Tight junction protein 2 Zona occludens protein 2 Zonula occludens protein 2 |
Gene namesi | Name:TJP2 Synonyms:ZO2 |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- tight junction By similarity
Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.By similarity
Nucleus
- nucleus Source: ARUK-UCL
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- bicellular tight junction Source: GO_Central
- cell surface Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Nucleus, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094542 | 1 – 1174 | Tight junction protein ZO-2Add BLAST | 1174 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 107 | PhosphoserineBy similarity | 1 | |
Modified residuei | 127 | PhosphoserineBy similarity | 1 | |
Modified residuei | 130 | PhosphoserineBy similarity | 1 | |
Modified residuei | 140 | PhosphoserineBy similarity | 1 | |
Modified residuei | 145 | PhosphoserineBy similarity | 1 | |
Modified residuei | 147 | PhosphoserineBy similarity | 1 | |
Modified residuei | 151 | PhosphoserineBy similarity | 1 | |
Modified residuei | 173 | PhosphoserineBy similarity | 1 | |
Modified residuei | 195 | PhosphoserineBy similarity | 1 | |
Modified residuei | 217 | PhosphoserineBy similarity | 1 | |
Modified residuei | 239 | PhosphoserineBy similarity | 1 | |
Modified residuei | 309 | PhosphoserineBy similarity | 1 | |
Modified residuei | 382 | PhosphoserineBy similarity | 1 | |
Modified residuei | 384 | PhosphoserineBy similarity | 1 | |
Modified residuei | 390 | PhosphoserineBy similarity | 1 | |
Modified residuei | 399 | PhosphoserineBy similarity | 1 | |
Modified residuei | 408 | PhosphoserineBy similarity | 1 | |
Modified residuei | 414 | PhosphoserineBy similarity | 1 | |
Modified residuei | 415 | PhosphoserineBy similarity | 1 | |
Modified residuei | 439 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 483 | PhosphoserineBy similarity | 1 | |
Modified residuei | 558 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 686 | PhosphoserineBy similarity | 1 | |
Modified residuei | 886 | PhosphoserineBy similarity | 1 | |
Modified residuei | 889 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 897 | PhosphoserineBy similarity | 1 | |
Modified residuei | 904 | PhosphoserineBy similarity | 1 | |
Modified residuei | 909 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 917 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 950 | PhosphoserineBy similarity | 1 | |
Modified residuei | 962 | PhosphoserineBy similarity | 1 | |
Modified residuei | 970 | PhosphoserineBy similarity | 1 | |
Modified residuei | 990 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1052 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1102 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1131 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1143 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated.
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q95168 |
PRIDEi | Q95168 |
PTM databases
iPTMneti | Q95168 |
Interactioni
Subunit structurei
Homodimer, and heterodimer with ZO1 and UBN1.
Interacts with SCRIB (By similarity).
Interacts with occludin and SAFB. Interaction with SAFB occurs in the nucleus.
Interacts with USP53 (via the C-terminal region) (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsQ95168
With | #Exp. | IntAct |
---|---|---|
Wwtr1 [Q9EPK5] from Mus musculus. | 4 | EBI-8304003,EBI-1211920 |
ZASP [E3Q1N2] from Homo sapiens. | 5 | EBI-8304003,EBI-8310421 |
GO - Molecular functioni
- cell adhesion molecule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 139773, 1 interactor |
IntActi | Q95168, 2 interactors |
MINTi | Q95168 |
STRINGi | 9612.ENSCAFP00000002794 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 97 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 291 – 369 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 493 – 574 | PDZ 3PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 588 – 653 | SH3PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 679 – 860 | Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST | 182 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1172 – 1174 | Interaction with SCRIBBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1146 – 1149 | Poly-Glu | 4 |
Sequence similaritiesi
Belongs to the MAGUK family.Curated
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG3580, Eukaryota |
InParanoidi | Q95168 |
OrthoDBi | 1175136at2759 |
Family and domain databases
CDDi | cd12027, SH3_ZO-2, 1 hit |
Gene3Di | 2.30.42.10, 3 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR005417, ZO IPR005419, ZO-2 IPR035598, ZO-2_SH3 |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 3 hits PF07653, SH3_2, 1 hit |
PRINTSi | PR01597, ZONOCCLUDNS PR01599, ZONOCCLUDNS2 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 3 hits |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 3 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 3 hits PS50002, SH3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q95168-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEELIWEQYT VTLQKDSKRG FGIAVSGGRD NPHFENGETS IVISDVLPGG
60 70 80 90 100
PADGLLQEND RVVMVNGTPM EDVLHSFAVQ QLRKSGKIAA IVVKRPRKVQ
110 120 130 140 150
LAPPQGSLPV DEDDRAFEVM DEFDGRSARS GYSERSRRSS HGGRSRSWED
160 170 180 190 200
SPERGRPHER AWSQERERSR GRSLERGLDH DDDYRRPRER SRGRSLERGL
210 220 230 240 250
DHDDDYGRPG ERSHGMSTDR GYDRGYDRGY DRGYDRTYSP EAEYGRRTQP
260 270 280 290 300
DARHAGSRSR SREHLRSRSP SPELRGRPDH AGQPDSDRPI GVLLMKSKAN
310 320 330 340 350
EEYGLRLGSQ IFIKQMTRTA LATKDGNLHE GDIILKINGT VTENMSLTDA
360 370 380 390 400
RKLIEKSRGK LQLVVLRDSK QTLINIPSLN DSDSEIEDIS EIESNRSFSP
410 420 430 440 450
EERRQQYSDY DYHSSNEKLK ERPNSREDMQ NRWSRMGATP TPFKSMGDIA
460 470 480 490 500
SVVGTENSKE PRYQEEPPAP QPKAAPRTFL RPSPEDEAIY GPNTKMVRFK
510 520 530 540 550
KGDSVGLRLA GGNDVGIFVA GIQEGTSAEQ EGLQEGDQIL KVNTQDFRGL
560 570 580 590 600
VREDAVLYLL EIPKGEMVTI LAQSRADVYR DILACGRGDS FFIRSHFECE
610 620 630 640 650
KETPQSLAFS RGEVFRVVDT LYDGKLGHWL AVRIGNELEK GLIPNKSRAE
660 670 680 690 700
QMASVQNAQR DNAGDRADFW RMRGQRSGMK KNLRKSREDL TAAVSVSTKF
710 720 730 740 750
PAYERVLLRE AGFKRPVVLF GPIADIALEK LANELPDLFQ TAKTEPKDAG
760 770 780 790 800
SEKSSGVVRL NTVRQIIEQD KHALLDVTPK AVDLLNYTQW FPIVIFFNPD
810 820 830 840 850
SRQGVKTMRQ RLNPTSNKSS RKLYDQANKL KKTCAHLFTA TINLNSANDS
860 870 880 890 900
WFGSLKDTIQ HQQGEAVWVS EGKMEGMDDD PEDRMSYLTA MGADYLSCDS
910 920 930 940 950
RLISDFEDTD GEGGAYTDNE LDEPAEEPLV SSITRSSEPV QHEESIRKPS
960 970 980 990 1000
PEPRAQMRRA ASRDQLRDSS PPPAFKPEPP KAKTQNREES FDISRSHDYK
1010 1020 1030 1040 1050
SNPSAVAGNE VSGASTRSCP PPIAAKPSFG RSILKPSTPV PSPESEEVGE
1060 1070 1080 1090 1100
GSEEQEGAPK SVLGKVKIFE KMDHKARLQR MQELQEAQNA RIEIAQKHPD
1110 1120 1130 1140 1150
IYAVPIKTHK PDPGLSQHTS SRPPEPQKGP SRLYQDPRGS YGSDAEEEEY
1160 1170
RQQLSEHSKR GYYSQPSRYR DTEL
Isoform 2 (identifier: Q95168-2)
Sequence is not available
Length:–
Mass (Da):–
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27152 mRNA Translation: AAC37332.1 |
RefSeqi | NP_001003204.1, NM_001003204.1 [Q95168-1] |
Genome annotation databases
GeneIDi | 403854 |
KEGGi | cfa:403854 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27152 mRNA Translation: AAC37332.1 |
RefSeqi | NP_001003204.1, NM_001003204.1 [Q95168-1] |
3D structure databases
SMRi | Q95168 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 139773, 1 interactor |
IntActi | Q95168, 2 interactors |
MINTi | Q95168 |
STRINGi | 9612.ENSCAFP00000002794 |
PTM databases
iPTMneti | Q95168 |
Proteomic databases
PaxDbi | Q95168 |
PRIDEi | Q95168 |
Genome annotation databases
GeneIDi | 403854 |
KEGGi | cfa:403854 |
Organism-specific databases
CTDi | 9414 |
Phylogenomic databases
eggNOGi | KOG3580, Eukaryota |
InParanoidi | Q95168 |
OrthoDBi | 1175136at2759 |
Family and domain databases
CDDi | cd12027, SH3_ZO-2, 1 hit |
Gene3Di | 2.30.42.10, 3 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR005417, ZO IPR005419, ZO-2 IPR035598, ZO-2_SH3 |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 3 hits PF07653, SH3_2, 1 hit |
PRINTSi | PR01597, ZONOCCLUDNS PR01599, ZONOCCLUDNS2 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 3 hits |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 3 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 3 hits PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZO2_CANLF | |
Accessioni | Q95168Primary (citable) accession number: Q95168 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families