UniProtKB - Q95114 (MFGM_BOVIN)
Lactadherin
MFGE8
Functioni
Contributes to phagocytic removal of apoptotic cells in many tissues. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity).
Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.
By similarity1 PublicationGO - Biological processi
- angiogenesis Source: UniProtKB-KW
- cell adhesion Source: UniProtKB-KW
- single fertilization Source: UniProtKB-KW
Keywordsi
Biological process | Angiogenesis, Cell adhesion, Fertilization |
Names & Taxonomyi
Protein namesi | Recommended name: LactadherinAlternative name(s): BP47 Components 15/16 MFGM MGP57/53 Milk fat globule-EGF factor 8 Short name: MFG-E8 PAS-6/PAS-7 glycoprotein SED1 Sperm surface protein SP47 |
Gene namesi | Name:MFGE8 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Other locations
- Membrane By similarity; Peripheral membrane protein By similarity
- acrosome membrane By similarity; Peripheral membrane protein By similarity
Note: Located in the acrosomal region of zona-pellucida bound sperm.By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- acrosomal membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasmic vesicle, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Add BLAST | 18 | |
ChainiPRO_0000007649 | 19 – 427 | LactadherinAdd BLAST | 409 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 24 ↔ 35 | By similarity | ||
Glycosylationi | 27 | O-linked (Fuc...) serine; in PAS-61 Publication | 1 | |
Disulfide bondi | 29 ↔ 47 | By similarity | ||
Glycosylationi | 34 | O-linked (Fuc...) threonine; in PAS-71 Publication | 1 | |
Disulfide bondi | 49 ↔ 58 | By similarity | ||
Glycosylationi | 59 | N-linked (GlcNAc...) (hybrid) asparagine; in PAS-6 and PAS-71 Publication | 1 | |
Disulfide bondi | 66 ↔ 77 | By similarity | ||
Disulfide bondi | 71 ↔ 94 | By similarity | ||
Disulfide bondi | 96 ↔ 105 | By similarity | ||
Disulfide bondi | 109 ↔ 265 | 1 Publication | ||
Glycosylationi | 227 | N-linked (GlcNAc...) (high mannose) asparagine; in PAS-61 Publication | 1 | |
Disulfide bondi | 252 ↔ 256 | 1 Publication | ||
Disulfide bondi | 270 ↔ 427 | 1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q95114 |
PeptideAtlasi | Q95114 |
PRIDEi | Q95114 |
PTM databases
iPTMneti | Q95114 |
Expressioni
Tissue specificityi
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q95114 |
SMRi | Q95114 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q95114 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 59 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 62 – 106 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 45 | |
Domaini | 109 – 265 | F5/8 type C 1PROSITE-ProRule annotationAdd BLAST | 157 | |
Domaini | 270 – 427 | F5/8 type C 2PROSITE-ProRule annotationAdd BLAST | 158 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 85 – 87 | Cell attachment site | 3 |
Domaini
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502RXUZ, Eukaryota |
InParanoidi | Q95114 |
Family and domain databases
CDDi | cd00057, FA58C, 2 hits |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000421, FA58C IPR008979, Galactose-bd-like_sf |
Pfami | View protein in Pfam PF00008, EGF, 2 hits PF00754, F5_F8_type_C, 2 hits |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00231, FA58C, 2 hits |
SUPFAMi | SSF49785, SSF49785, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01186, EGF_2, 2 hits PS50026, EGF_3, 2 hits PS01285, FA58C_1, 2 hits PS01286, FA58C_2, 2 hits PS50022, FA58C_3, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPCPRLLAAL FCSSGLFAAS GDFCDSSLCL HGGTCLLNED RTPPFYCLCP
60 70 80 90 100
EGFTGLLCNE TEHGPCFPNP CHNDAECQVT DDSHRGDVFI QYICKCPLGY
110 120 130 140 150
VGIHCETTCT SPLGMQTGAI ADSQISASSM HLGFMGLQRW APELARLHQT
160 170 180 190 200
GIVNAWTSGN YDKNPWIQVN LMRKMWVTGV VTQGASRAGS AEYLKTFKVA
210 220 230 240 250
YSTDGRQFQF IQVAGRSGDK IFIGNVNNSG LKINLFDTPL ETQYVRLVPI
260 270 280 290 300
ICHRGCTLRF ELLGCELNGC TEPLGLKDNT IPNKQITASS YYKTWGLSAF
310 320 330 340 350
SWFPYYARLD NQGKFNAWTA QTNSASEWLQ IDLGSQKRVT GIITQGARDF
360 370 380 390 400
GHIQYVAAYR VAYGDDGVTW TEYKDPGASE SKIFPGNMDN NSHKKNIFET
410 420
PFQARFVRIQ PVAWHNRITL RVELLGC
The sequence of this isoform differs from the canonical sequence as follows:
169-221: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | A → F in CAA62997 (PubMed:8856064).Curated | 1 | |
Sequence conflicti | 28 | L → Q in CAA62997 (PubMed:8856064).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001398 | 169 – 221 | Missing in isoform Short. CuratedAdd BLAST | 53 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X91895 mRNA Translation: CAA62997.1 S80643 mRNA Translation: AAB35894.2 Y11719 mRNA Translation: CAA72406.1 |
PIRi | S74211 |
RefSeqi | NP_788783.1, NM_176610.1 |
Genome annotation databases
GeneIDi | 281913 |
KEGGi | bta:281913 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X91895 mRNA Translation: CAA62997.1 S80643 mRNA Translation: AAB35894.2 Y11719 mRNA Translation: CAA72406.1 |
PIRi | S74211 |
RefSeqi | NP_788783.1, NM_176610.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PQS | X-ray | 2.40 | A/B/C/D | 270-427 | [»] | |
3BN6 | X-ray | 1.67 | A | 270-427 | [»] | |
AlphaFoldDBi | Q95114 | |||||
SMRi | Q95114 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | Q95114 |
STRINGi | 9913.ENSBTAP00000004272 |
PTM databases
iPTMneti | Q95114 |
Proteomic databases
PaxDbi | Q95114 |
PeptideAtlasi | Q95114 |
PRIDEi | Q95114 |
Genome annotation databases
GeneIDi | 281913 |
KEGGi | bta:281913 |
Organism-specific databases
CTDi | 4240 |
Phylogenomic databases
eggNOGi | ENOG502RXUZ, Eukaryota |
InParanoidi | Q95114 |
Miscellaneous databases
EvolutionaryTracei | Q95114 |
Family and domain databases
CDDi | cd00057, FA58C, 2 hits |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000421, FA58C IPR008979, Galactose-bd-like_sf |
Pfami | View protein in Pfam PF00008, EGF, 2 hits PF00754, F5_F8_type_C, 2 hits |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00231, FA58C, 2 hits |
SUPFAMi | SSF49785, SSF49785, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01186, EGF_2, 2 hits PS50026, EGF_3, 2 hits PS01285, FA58C_1, 2 hits PS01286, FA58C_2, 2 hits PS50022, FA58C_3, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | MFGM_BOVIN | |
Accessioni | Q95114Primary (citable) accession number: Q95114 Secondary accession number(s): P79344, Q27959 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references