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Protein

SUMO-conjugating enzyme UBC9

Gene

ubc-9

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like protein smo-1 from the aos-1-uba-2 E1 complex and catalyzes its covalent attachment to other proteins with the help of an E3 ligase such as gei-17. Required to sumoylate the ETS transcription factor lin-1 and the Polycomb protein sop-2. Required for embryonic development, fertility, vulval morphogenesis and inhibition of vulval cell fates.6 Publications

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • RNA polymerase II transcription factor binding Source: WormBase
  • SAM domain binding Source: WormBase
  • SUMO conjugating enzyme activity Source: WormBase
  • SUMO transferase activity Source: WormBase

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-3065678 SUMO is transferred from E1 to E2 (UBE2I, UBC9)
R-CEL-3108214 SUMOylation of DNA damage response and repair proteins
R-CEL-3232118 SUMOylation of transcription factors
R-CEL-3899300 SUMOylation of transcription cofactors
R-CEL-4085377 SUMOylation of SUMOylation proteins
R-CEL-4090294 SUMOylation of intracellular receptors
R-CEL-4551638 SUMOylation of chromatin organization proteins
R-CEL-4570464 SUMOylation of RNA binding proteins
R-CEL-4615885 SUMOylation of DNA replication proteins
R-CEL-5696395 Formation of Incision Complex in GG-NER
R-CEL-8866904 Negative regulation of activity of TFAP2 (AP-2) family transcription factors
UniPathwayi
UPA00886

Protein family/group databases

MoonDBiQ95017 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzyme UBC9 (EC:2.3.2.-)
Alternative name(s):
RING-type E3 SUMO transferase UBC9
SUMO-protein ligase
Ubiquitin carrier protein 9
Ubiquitin-conjugating enzyme E2 9
Ubiquitin-protein ligase 9
Gene namesi
Name:ubc-9
ORF Names:F29B9.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF29B9.6 ; CE09784 ; WBGene00006706 ; ubc-9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in sterility, embryonic lethality and sensitivity to radiation (PubMed:14711411). RNAi-mediated knockdown causes a number of defects during the first embryonic mitotic division including loss of smo-1 from metaphase chromosomes, chromosome misalignment at metaphase, diminished spindle pole separation, slow chromosome segregation, decreased distance between chromosomes afer anaphase onset and increased air-2 levels in the spindle midzone (PubMed:25475837).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825171 – 166SUMO-conjugating enzyme UBC9Add BLAST166

Proteomic databases

EPDiQ95017
PaxDbiQ95017
PeptideAtlasiQ95017
PRIDEiQ95017

Expressioni

Gene expression databases

BgeeiWBGene00006706 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Interactioni

Subunit structurei

Interacts with brd-1 and rad-51 (PubMed:14711411). Interacts with smo-1 and sop-2 (Ref. 1, PubMed:15107848). Interacts with bet-1 (via BROMO domain 2) (PubMed:24349540).3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi533085, 8 interactors
DIPiDIP-25778N
IntActiQ95017, 10 interactors
MINTiQ95017
STRINGi6239.F29B9.6.1

Structurei

3D structure databases

ProteinModelPortaliQ95017
SMRiQ95017
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0424 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00550000075088
HOGENOMiHOG000233454
InParanoidiQ95017
KOiK10577
OMAiKCKFTPP
OrthoDBiEOG091G0NAK
PhylomeDBiQ95017

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR027230 Ubc9
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR43927 PTHR43927, 1 hit
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q95017-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGIAAGRLA EERKHWRKDH PFGFIAKPVK NADGTLNLFN WECAIPGRKD
60 70 80 90 100
TIWEGGLYRI RMLFKDDFPS TPPKCKFEPP LFHPNVYPSG TVCLSLLDEN
110 120 130 140 150
KDWKPSISIK QLLIGIQDLL NHPNIEDPAQ AEAYQIYCQN RAEYEKRVKK
160
EAVKYAAELV QKQMLE
Length:166
Mass (Da):19,115
Last modified:February 1, 1997 - v1
Checksum:iE5A5AFA773DE286C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106565 mRNA Translation: AAC97374.1
FO081255 Genomic DNA Translation: CCD70229.1
PIRiT29929
RefSeqiNP_001023158.1, NM_001027987.3
UniGeneiCel.38991

Genome annotation databases

EnsemblMetazoaiF29B9.6.1; F29B9.6.1; WBGene00006706
F29B9.6.2; F29B9.6.2; WBGene00006706
GeneIDi3565767
KEGGicel:CELE_F29B9.6
UCSCiF29B9.6.1 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106565 mRNA Translation: AAC97374.1
FO081255 Genomic DNA Translation: CCD70229.1
PIRiT29929
RefSeqiNP_001023158.1, NM_001027987.3
UniGeneiCel.38991

3D structure databases

ProteinModelPortaliQ95017
SMRiQ95017
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi533085, 8 interactors
DIPiDIP-25778N
IntActiQ95017, 10 interactors
MINTiQ95017
STRINGi6239.F29B9.6.1

Protein family/group databases

MoonDBiQ95017 Predicted

Proteomic databases

EPDiQ95017
PaxDbiQ95017
PeptideAtlasiQ95017
PRIDEiQ95017

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF29B9.6.1; F29B9.6.1; WBGene00006706
F29B9.6.2; F29B9.6.2; WBGene00006706
GeneIDi3565767
KEGGicel:CELE_F29B9.6
UCSCiF29B9.6.1 c. elegans

Organism-specific databases

CTDi3565767
WormBaseiF29B9.6 ; CE09784 ; WBGene00006706 ; ubc-9

Phylogenomic databases

eggNOGiKOG0424 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00550000075088
HOGENOMiHOG000233454
InParanoidiQ95017
KOiK10577
OMAiKCKFTPP
OrthoDBiEOG091G0NAK
PhylomeDBiQ95017

Enzyme and pathway databases

UniPathwayi
UPA00886

ReactomeiR-CEL-3065678 SUMO is transferred from E1 to E2 (UBE2I, UBC9)
R-CEL-3108214 SUMOylation of DNA damage response and repair proteins
R-CEL-3232118 SUMOylation of transcription factors
R-CEL-3899300 SUMOylation of transcription cofactors
R-CEL-4085377 SUMOylation of SUMOylation proteins
R-CEL-4090294 SUMOylation of intracellular receptors
R-CEL-4551638 SUMOylation of chromatin organization proteins
R-CEL-4570464 SUMOylation of RNA binding proteins
R-CEL-4615885 SUMOylation of DNA replication proteins
R-CEL-5696395 Formation of Incision Complex in GG-NER
R-CEL-8866904 Negative regulation of activity of TFAP2 (AP-2) family transcription factors

Miscellaneous databases

PROiPR:Q95017

Gene expression databases

BgeeiWBGene00006706 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR027230 Ubc9
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR43927 PTHR43927, 1 hit
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBC9_CAEEL
AccessioniPrimary (citable) accession number: Q95017
Secondary accession number(s): Q9GYI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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