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Protein

Probable delta-aminolevulinic acid dehydratase 2, chloroplastic

Gene

HEMB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as an allosteric activator.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable delta-aminolevulinic acid dehydratase 2, chloroplastic (HEMB2), Delta-aminolevulinic acid dehydratase 1, chloroplastic (HEMB1)
  2. Porphobilinogen deaminase, chloroplastic (HEMC)
  3. Uroporphyrinogen-III synthase, chloroplastic (UROS)
  4. Uroporphyrinogen decarboxylase 2, chloroplastic (HEME2), Uroporphyrinogen decarboxylase (AXX17_At3g15570), Uroporphyrinogen decarboxylase (At2g40490), Uroporphyrinogen decarboxylase (AXX17_At2g37600), Uroporphyrinogen decarboxylase 1, chloroplastic (HEME1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei275Schiff-base intermediate with substrateBy similarity1
Binding sitei285Substrate 1By similarity1
Binding sitei291Substrate 1By similarity1
Metal bindingi307MagnesiumBy similarity1
Active sitei322Schiff-base intermediate with substrateBy similarity1
Binding sitei348Substrate 2By similarity1
Binding sitei387Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processChlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G44318-MONOMER
ReactomeiR-ATH-189451 Heme biosynthesis
R-ATH-6798695 Neutrophil degranulation
UniPathwayiUPA00251; UER00318
UPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Probable delta-aminolevulinic acid dehydratase 2, chloroplastic (EC:4.2.1.24)
Short name:
ALADH2
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB2
Ordered Locus Names:At1g44318
ORF Names:T18F15.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G44318
TAIRilocus:2823624 AT1G44318

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34ChloroplastSequence analysisAdd BLAST34
ChainiPRO_000042267235 – 406Probable delta-aminolevulinic acid dehydratase 2, chloroplasticAdd BLAST372

Proteomic databases

PaxDbiQ94LA4
PRIDEiQ94LA4
ProMEXiQ94LA4

Expressioni

Gene expression databases

ExpressionAtlasiQ94LA4 baseline and differential
GenevisibleiQ94LA4 AT

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G44318.1

Structurei

3D structure databases

ProteinModelPortaliQ94LA4
SMRiQ94LA4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2794 Eukaryota
COG0113 LUCA
HOGENOMiHOG000020323
InParanoidiQ94LA4
KOiK01698
OMAiGFEQKSY
OrthoDBiEOG09360FP1
PhylomeDBiQ94LA4

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR001731 ALAD
IPR013785 Aldolase_TIM
PANTHERiPTHR11458 PTHR11458, 1 hit
PfamiView protein in Pfam
PF00490 ALAD, 1 hit
PRINTSiPR00144 DALDHYDRTASE
SMARTiView protein in SMART
SM01004 ALAD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94LA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSMFRSPC KIPSVKGFEQ KSYVGLKAAS YNVRVNSFKS SEVASQLQKI
60 70 80 90 100
DSTLIWPVNA LEAPPVPSKP ATPLIDQPLQ LSRRARRNRK CPTQRAAFQE
110 120 130 140 150
TNISPANFIY PLFIHEGEVD IPITSMPGRY MLGWRHGLIE EVARALDVGV
160 170 180 190 200
NSVKLYPKVP EALKSPTGEE AFNDNGLIPR TVRLLKDRFP DLVIYTDVNF
210 220 230 240 250
DEYSTTGHGG IVGEDGVILN DETIHQLRKQ AVSQARAGAD VVCTSEMLDG
260 270 280 290 300
RVGAVRAALD AEGFQDVSIM SYSVKYTSSL YGRFRKVQLD KKTYQINPAN
310 320 330 340 350
SREALLEARE DEAEGADILM VKPALPSLDI IRLLKNQTLL PIGACQVSGE
360 370 380 390 400
YSMIKAAGLL KMIDEEKVMM ESLLCIRRAG ADLILTYFAL QAATKLCGEN

KRFSSN
Length:406
Mass (Da):44,878
Last modified:December 1, 2001 - v1
Checksum:i47ED3AD6F1ED0A91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084807 Genomic DNA Translation: AAK43479.1
CP002684 Genomic DNA Translation: AEE32031.1
RefSeqiNP_175085.1, NM_103545.2

Genome annotation databases

EnsemblPlantsiAT1G44318.1; AT1G44318.1; AT1G44318
GeneIDi841025
GrameneiAT1G44318.1; AT1G44318.1; AT1G44318
KEGGiath:AT1G44318

Similar proteinsi

Entry informationi

Entry nameiHEM22_ARATH
AccessioniPrimary (citable) accession number: Q94LA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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