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Protein

Pyruvate dehydrogenase [NADP(+)], mitochondrial

Gene

PNO

Organism
Euglena gracilis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pyruvate dehydrogenase [NADP+] is one of three enzymes participating in respiratory metabolism. The enzyme is also active with 2-oxobutyrate and oxaloacetate. The enzyme is oxygen sensitive.2 Publications
Aquacobalamin reductase is involved in the synthesis of cobalamin coenzymes.2 Publications

Miscellaneous

Arose from gene fusion of pyruvate:ferredoxin oxidoreductase and cytochrome-P450 reductase. Gene fusion has only been found in Euglena and Cryptosporidium.2 Publications
Euglena cells absolutely require thiamine for growth due to the lack of a pyrimidine formation biosynthetic pathway. Thiamine is actively taken up into the cells and is used as a cofactor after being converted to TPP (thiamine pyrophosphate).1 Publication

Catalytic activityi

Pyruvate + CoA + NADP+ = acetyl-CoA + CO2 + NADPH.1 Publication
2 cob(II)alamin + NADP+ + 2 H2O = 2 aquacob(III)alamin + NADPH.1 Publication

Cofactori

Protein has several cofactor binding sites:

Kineticsi

  1. KM=210 µM for pyruvate1 Publication
  2. KM=8 µM for CoA1 Publication
  3. KM=30 µM for NADP+1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi756Iron-sulfur 1 (4Fe-4S)Sequence analysis1
    Metal bindingi759Iron-sulfur 1 (4Fe-4S)Sequence analysis1
    Metal bindingi762Iron-sulfur 1 (4Fe-4S)Sequence analysis1
    Metal bindingi766Iron-sulfur 1 (4Fe-4S)Sequence analysis1
    Metal bindingi811Iron-sulfur 2 (4Fe-4S)Sequence analysis1
    Metal bindingi814Iron-sulfur 2 (4Fe-4S)Sequence analysis1
    Metal bindingi817Iron-sulfur 2 (4Fe-4S)Sequence analysis1
    Metal bindingi821Iron-sulfur 2 (4Fe-4S)Sequence analysis1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi1458 – 1469FADBy similarityAdd BLAST12
    Nucleotide bindingi1585 – 1595FADBy similarityAdd BLAST11

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processElectron transport, Transport
    Ligand4Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NADP, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17049
    BRENDAi1.2.1.51 2197
    SABIO-RKiQ94IN5

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase [NADP(+)], mitochondrial (EC:1.16.1.51 Publication, EC:1.2.1.511 Publication)
    Alternative name(s):
    Aquacobalamin reductase [NADPH]
    EgPNOmt
    Pyruvate:NADP(+) oxidoreductase
    Gene namesi
    Name:PNO
    OrganismiEuglena gracilisImported
    Taxonomic identifieri3039 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaEuglenidaEuglenalesEuglenaceaeEuglena

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 37Mitochondrion1 PublicationAdd BLAST37
    ChainiPRO_000002386738 – 1803Pyruvate dehydrogenase [NADP(+)], mitochondrialAdd BLAST1766

    Proteomic databases

    PRIDEiQ94IN5

    Expressioni

    Inductioni

    Expressed in both aerobic and anaerobic conditions.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ94IN5
    SMRiQ94IN5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini747 – 7764Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
    Domaini802 – 8314Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30
    Domaini1248 – 1391Flavodoxin-likePROSITE-ProRule annotationAdd BLAST144
    Domaini1425 – 1650FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST226

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    KOiK21682

    Family and domain databases

    Gene3Di1.20.990.10, 1 hit
    3.40.50.360, 1 hit
    3.40.50.80, 1 hit
    3.40.50.920, 1 hit
    3.40.920.10, 1 hit
    4.10.780.10, 1 hit
    InterProiView protein in InterPro
    IPR017896 4Fe4S_Fe-S-bd
    IPR017900 4Fe4S_Fe_S_CS
    IPR003097 CysJ-like_FAD-binding
    IPR017927 FAD-bd_FR_type
    IPR001094 Flavdoxin-like
    IPR008254 Flavodoxin/NO_synth
    IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
    IPR029039 Flavoprotein-like_sf
    IPR039261 FNR_nucleotide-bd
    IPR023173 NADPH_Cyt_P450_Rdtase_alpha
    IPR001433 OxRdtase_FAD/NAD-bd
    IPR033412 PFOR_II
    IPR037112 Pyrv-flavodox_OxR_EKR_sf
    IPR019456 Pyrv-flavodox_OxRtase_EKR
    IPR019752 Pyrv/ketoisovalerate_OxRed_cat
    IPR002880 Pyrv_Fd/Flavodoxin_OxRdtase_N
    IPR011895 Pyrv_flavodox_OxRed
    IPR002869 Pyrv_flavodox_OxRed_cen
    IPR017938 Riboflavin_synthase-like_b-brl
    IPR029061 THDP-binding
    IPR011766 TPP_enzyme-bd_C
    IPR009014 Transketo_C/PFOR_II
    PfamiView protein in Pfam
    PF10371 EKR, 1 hit
    PF00667 FAD_binding_1, 1 hit
    PF12838 Fer4_7, 1 hit
    PF00258 Flavodoxin_1, 1 hit
    PF00175 NAD_binding_1, 1 hit
    PF17147 PFOR_II, 1 hit
    PF01558 POR, 1 hit
    PF01855 POR_N, 1 hit
    PF02775 TPP_enzyme_C, 1 hit
    PRINTSiPR00369 FLAVODOXIN
    PR00371 FPNCR
    SMARTiView protein in SMART
    SM00890 EKR, 1 hit
    SUPFAMiSSF52218 SSF52218, 1 hit
    SSF52343 SSF52343, 1 hit
    SSF52518 SSF52518, 2 hits
    SSF52922 SSF52922, 1 hit
    SSF53323 SSF53323, 1 hit
    SSF63380 SSF63380, 1 hit
    TIGRFAMsiTIGR02176 pyruv_ox_red, 1 hit
    PROSITEiView protein in PROSITE
    PS00198 4FE4S_FER_1, 2 hits
    PS51379 4FE4S_FER_2, 2 hits
    PS51384 FAD_FR, 1 hit
    PS50902 FLAVODOXIN_LIKE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q94IN5-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKQSVRPIIS NVLRKEVALY STIIGQDKGK EPTGRTYTSG PKPASHIEVP
    60 70 80 90 100
    HHVTVPATDR TPNPDAQFFQ SVDGSQATSH VAYALSDTAF IYPITPSSVM
    110 120 130 140 150
    GELADVWMAQ GRKNAFGQVV DVREMQSEAG AAGALHGALA AGAIATTFTA
    160 170 180 190 200
    SQGLLLMIPN MYKIAGELMP SVIHVAAREL AGHALSIFGG HADVMAVRQT
    210 220 230 240 250
    GWAMLCSHTV QQSHDMALIS HVATLKSSIP FVHFFDGFRT SHEVNKIKML
    260 270 280 290 300
    PYAELKKLVP PGTMEQHWAR SLNPMHPTIR GTNQSADIYF QNMESANQYY
    310 320 330 340 350
    TDLAEVVQET MDEVAPYIGR HYKIFEYVGA PDAEEVTVLM GSGATTVNEA
    360 370 380 390 400
    VDLLVKRGKK VGAVLVHLYR PWSTKAFEKV LPKTVKRIAA LDRCKEVTAL
    410 420 430 440 450
    GEPLYLDVSA TLNLFPERQN VKVIGGRYGL GSKDFIPEHA LAIYANLASE
    460 470 480 490 500
    NPIQRFTVGI TDDVTGTSVP FVNERVDTLP EGTRQCVFWG IGSDGTVGAN
    510 520 530 540 550
    RSAVRIIGDN SDLMVQAYFQ FDAFKSGGVT SSHLRFGPKP ITAQYLVTNA
    560 570 580 590 600
    DYIACHFQEY VKRFDMLDAI REGGTFVLNS RWTTEDMEKE IPADFRRNVA
    610 620 630 640 650
    QKKVRFYNVD ARKICDSFGL GKRINMLMQA CFFKLSGVLP LAEAQRLLNE
    660 670 680 690 700
    SIVHEYGKKG GKVVEMNQAV VNAVFAGDLP QEVQVPAAWA NAVDTSTRTP
    710 720 730 740 750
    TGIEFVDKIM RPLMDFKGDQ LPVSVMTPGG TFPVGTTQYA KRAIAAFIPQ
    760 770 780 790 800
    WIPANCTQCN YCSYVCPHAT IRPFVLTDQE VQLAPESFVT RKAKGDYQGM
    810 820 830 840 850
    NFRIQVAPED CTGCQVCVET CPDDALEMTD AFTATPVQRT NWEFAIKVPN
    860 870 880 890 900
    RGTMTDRYSL KGSQFQQPLL EFSGACEGCG ETPYVKLLTQ LFGERTVIAN
    910 920 930 940 950
    ATGCSSIWGG TAGLAPYTTN AKGQGPAWGN SLFEDNAEFG FGIAVANAQK
    960 970 980 990 1000
    RSRVRDCILQ AVEKKVADEG LTTLLAQWLQ DWNTGDKTLK YQDQIIAGLA
    1010 1020 1030 1040 1050
    QQRSKDPLLE QIYGMKDMLP NISQWIIGGD GWANDIGFGG LDHVLASGQN
    1060 1070 1080 1090 1100
    LNVLVLDTEM YSNTGGQASK STHMASVAKF ALGGKRTNKK NLTEMAMSYG
    1110 1120 1130 1140 1150
    NVYVATVSHG NMAQCVKAFV EAESYDGPSL IVGYAPCIEH GLRAGMARMV
    1160 1170 1180 1190 1200
    QESEAAIATG YWPLYRFDPR LATEGKNPFQ LDSKRIKGNL QEYLDRQNRY
    1210 1220 1230 1240 1250
    VNLKKNNPKG ADLLKSQMAD NITARFNRYR RMLEGPNTKA AAPSGNHVTI
    1260 1270 1280 1290 1300
    LYGSETGNSE GLAKELATDF ERREYSVAVQ ALDDIDVADL ENMGFVVIAV
    1310 1320 1330 1340 1350
    STCGQGQFPR NSQLFWRELQ RDKPEGWLKN LKYTVFGLGD STYYFYCHTA
    1360 1370 1380 1390 1400
    KQIDARLAAL GAQRVVPIGF GDDGDEDMFH TGFNNWIPSV WNELKTKTPE
    1410 1420 1430 1440 1450
    EALFTPSIAV QLTPNATPQD FHFAKSTPVL SITGAERITP ADHTRNFVTI
    1460 1470 1480 1490 1500
    RWKTDLSYQV GDSLGVFPEN TRSVVEEFLQ YYGLNPKDVI TIENKGSREL
    1510 1520 1530 1540 1550
    PHCMAVGDLF TKVLDILGKP NNRFYKTLSY FAVDKAEKER LLKIAEMGPE
    1560 1570 1580 1590 1600
    YSNILSEMYH YADIFHMFPS ARPTLQYLIE MIPNIKPRYY SISSAPIHTP
    1610 1620 1630 1640 1650
    GEVHSLVLID TWITLSGKHR TGLTCTMLEH LQAGQVVDGC IHPTAMEFPD
    1660 1670 1680 1690 1700
    HEKPVVMCAM GSGLAPFVAF LRERSTLRKQ GKKTGNMALY FGNRYEKTEF
    1710 1720 1730 1740 1750
    LMKEELKGHI NDGLLTLRCA FSRDDPKKKV YVQDLIKMDE KMMYDYLVVQ
    1760 1770 1780 1790 1800
    KGSMYCCGSR SFIKPVQESL KHCFMKAGGL TAEQAENEVI DMFTTGRYNI

    EAW
    Length:1,803
    Mass (Da):199,821
    Last modified:December 1, 2001 - v1
    Checksum:i5AFB6E3869CADCC6
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti50P → S AA sequence (PubMed:1910287).Curated1
    Sequence conflicti52H → A AA sequence (PubMed:1910287).Curated1
    Sequence conflicti53V → K AA sequence (PubMed:1910287).Curated1
    Sequence conflicti598 – 599NV → KL in BAB12024 (PubMed:11023353).Curated2
    Sequence conflicti1256T → E AA sequence (PubMed:1910287).Curated1
    Sequence conflicti1558M → T in BAB12024 (PubMed:11023353).Curated1
    Sequence conflicti1673 – 1674ER → DG in BAB12024 (PubMed:11023353).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB021127 mRNA Translation: BAB12024.1
    AJ278425 mRNA Translation: CAC37628.1
    PIRiS36876

    Genome annotation databases

    KEGGiag:BAB12024

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB021127 mRNA Translation: BAB12024.1
    AJ278425 mRNA Translation: CAC37628.1
    PIRiS36876

    3D structure databases

    ProteinModelPortaliQ94IN5
    SMRiQ94IN5
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PRIDEiQ94IN5

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:BAB12024

    Phylogenomic databases

    KOiK21682

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17049
    BRENDAi1.2.1.51 2197
    SABIO-RKiQ94IN5

    Family and domain databases

    Gene3Di1.20.990.10, 1 hit
    3.40.50.360, 1 hit
    3.40.50.80, 1 hit
    3.40.50.920, 1 hit
    3.40.920.10, 1 hit
    4.10.780.10, 1 hit
    InterProiView protein in InterPro
    IPR017896 4Fe4S_Fe-S-bd
    IPR017900 4Fe4S_Fe_S_CS
    IPR003097 CysJ-like_FAD-binding
    IPR017927 FAD-bd_FR_type
    IPR001094 Flavdoxin-like
    IPR008254 Flavodoxin/NO_synth
    IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
    IPR029039 Flavoprotein-like_sf
    IPR039261 FNR_nucleotide-bd
    IPR023173 NADPH_Cyt_P450_Rdtase_alpha
    IPR001433 OxRdtase_FAD/NAD-bd
    IPR033412 PFOR_II
    IPR037112 Pyrv-flavodox_OxR_EKR_sf
    IPR019456 Pyrv-flavodox_OxRtase_EKR
    IPR019752 Pyrv/ketoisovalerate_OxRed_cat
    IPR002880 Pyrv_Fd/Flavodoxin_OxRdtase_N
    IPR011895 Pyrv_flavodox_OxRed
    IPR002869 Pyrv_flavodox_OxRed_cen
    IPR017938 Riboflavin_synthase-like_b-brl
    IPR029061 THDP-binding
    IPR011766 TPP_enzyme-bd_C
    IPR009014 Transketo_C/PFOR_II
    PfamiView protein in Pfam
    PF10371 EKR, 1 hit
    PF00667 FAD_binding_1, 1 hit
    PF12838 Fer4_7, 1 hit
    PF00258 Flavodoxin_1, 1 hit
    PF00175 NAD_binding_1, 1 hit
    PF17147 PFOR_II, 1 hit
    PF01558 POR, 1 hit
    PF01855 POR_N, 1 hit
    PF02775 TPP_enzyme_C, 1 hit
    PRINTSiPR00369 FLAVODOXIN
    PR00371 FPNCR
    SMARTiView protein in SMART
    SM00890 EKR, 1 hit
    SUPFAMiSSF52218 SSF52218, 1 hit
    SSF52343 SSF52343, 1 hit
    SSF52518 SSF52518, 2 hits
    SSF52922 SSF52922, 1 hit
    SSF53323 SSF53323, 1 hit
    SSF63380 SSF63380, 1 hit
    TIGRFAMsiTIGR02176 pyruv_ox_red, 1 hit
    PROSITEiView protein in PROSITE
    PS00198 4FE4S_FER_1, 2 hits
    PS51379 4FE4S_FER_2, 2 hits
    PS51384 FAD_FR, 1 hit
    PS50902 FLAVODOXIN_LIKE, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPNO_EUGGR
    AccessioniPrimary (citable) accession number: Q94IN5
    Secondary accession number(s): Q9FZP8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
    Last sequence update: December 1, 2001
    Last modified: October 10, 2018
    This is version 107 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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