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Protein

DNA (cytosine-5)-methyltransferase CMT3

Gene

CMT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the CpXpG methylation and in gene silencing. Methylates preferentially transposon-related sequences. Functionally redundant to DRM1/DRM2 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.4 Publications

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei460PROSITE-ProRule annotation1

GO - Molecular functioni

  • chromatin binding Source: InterPro
  • DNA (cytosine-5-)-methyltransferase activity Source: TAIR
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • chromatin silencing Source: TAIR
  • DNA methylation Source: TAIR
  • DNA methylation on cytosine within a CNG sequence Source: TAIR
  • histone H3-K9 methylation Source: TAIR
  • negative regulation of gene expression, epigenetic Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
  • zygote asymmetric cytokinesis in embryo sac Source: TAIR

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Methyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.37 399

Protein family/group databases

REBASEi4853 M.AthCMT3

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase CMT3 (EC:2.1.1.37)
Alternative name(s):
Chromomethylase 3
Protein CHROMOMETHYLASE 3
Gene namesi
Name:CMT3
Ordered Locus Names:At1g69770
ORF Names:T6C23.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G69770
TAIRilocus:2205015 AT1G69770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi279G → E: Loss of activity. 1 Publication1
Mutagenesisi382F → A: Loss of binding to H3. 1 Publication1
Mutagenesisi456G → D: Loss of activity. 1 Publication1
Mutagenesisi465G → D: Loss of activity. 1 Publication1
Mutagenesisi470R → K in cmt3-10; partial loss of activity. 1 Publication1
Mutagenesisi541G → E in cmt3-6; loss of activity. 1 Publication1
Mutagenesisi542L → F: Loss of activity. 1 Publication1
Mutagenesisi683R → K: Loss of activity. 1 Publication1
Mutagenesisi724G → E in cmt3-4; loss of activity. 1 Publication1
Mutagenesisi733L → F: Loss of activity. 1 Publication1
Mutagenesisi748P → L: Loss of activity. 1 Publication1
Mutagenesisi763S → F in cmt3-8; partial loss of activity. 1 Publication1
Mutagenesisi769R → K in cmt3-3; loss of activity. 1 Publication1
Mutagenesisi807G → R: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002466931 – 839DNA (cytosine-5)-methyltransferase CMT3Add BLAST839

Proteomic databases

PaxDbiQ94F88
PRIDEiQ94F88

Expressioni

Gene expression databases

ExpressionAtlasiQ94F88 baseline and differential
GenevisibleiQ94F88 AT

Interactioni

Subunit structurei

Homodimer. Interacts with HP1 and, through its chromodomain, with the N-terminal tail of histone H3 doubly methylated at 'Lys-9' and 'Lys-27'.2 Publications

Protein-protein interaction databases

BioGridi28534, 3 interactors
DIPiDIP-60719N
STRINGi3702.AT1G69770.1

Structurei

3D structure databases

ProteinModelPortaliQ94F88
SMRiQ94F88
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 227BAHPROSITE-ProRule annotationAdd BLAST120
Domaini269 – 813SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST545
Domaini382 – 447ChromoPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH5K Eukaryota
COG0270 LUCA
HOGENOMiHOG000082844
InParanoidiQ94F88
KOiK00558
OMAiYDHRPLE
OrthoDBiEOG0936054M
PhylomeDBiQ94F88

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR001025 BAH_dom
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR023780 Chromo_domain
IPR023779 Chromodomain_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01426 BAH, 1 hit
PF00385 Chromo, 1 hit
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SMARTiView protein in SMART
SM00439 BAH, 1 hit
SM00298 CHROMO, 1 hit
SUPFAMiSSF53335 SSF53335, 4 hits
SSF54160 SSF54160, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS51038 BAH, 1 hit
PS00094 C5_MTASE_1, 1 hit
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

Q94F88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKRKRPAT KDDTTKSIPK PKKRAPKRAK TVKEEPVTVV EEGEKHVARF
60 70 80 90 100
LDEPIPESEA KSTWPDRYKP IEVQPPKASS RKKTKDDEKV EIIRARCHYR
110 120 130 140 150
RAIVDERQIY ELNDDAYVQS GEGKDPFICK IIEMFEGANG KLYFTARWFY
160 170 180 190 200
RPSDTVMKEF EILIKKKRVF FSEIQDTNEL GLLEKKLNIL MIPLNENTKE
210 220 230 240 250
TIPATENCDF FCDMNYFLPY DTFEAIQQET MMAISESSTI SSDTDIREGA
260 270 280 290 300
AAISEIGECS QETEGHKKAT LLDLYSGCGA MSTGLCMGAQ LSGLNLVTKW
310 320 330 340 350
AVDMNAHACK SLQHNHPETN VRNMTAEDFL FLLKEWEKLC IHFSLRNSPN
360 370 380 390 400
SEEYANLHGL NNVEDNEDVS EESENEDDGE VFTVDKIVGI SFGVPKKLLK
410 420 430 440 450
RGLYLKVRWL NYDDSHDTWE PIEGLSNCRG KIEEFVKLGY KSGILPLPGG
460 470 480 490 500
VDVVCGGPPC QGISGHNRFR NLLDPLEDQK NKQLLVYMNI VEYLKPKFVL
510 520 530 540 550
MENVVDMLKM AKGYLARFAV GRLLQMNYQV RNGMMAAGAY GLAQFRLRFF
560 570 580 590 600
LWGALPSEII PQFPLPTHDL VHRGNIVKEF QGNIVAYDEG HTVKLADKLL
610 620 630 640 650
LKDVISDLPA VANSEKRDEI TYDKDPTTPF QKFIRLRKDE ASGSQSKSKS
660 670 680 690 700
KKHVLYDHHP LNLNINDYER VCQVPKRKGA NFRDFPGVIV GPGNVVKLEE
710 720 730 740 750
GKERVKLESG KTLVPDYALT YVDGKSCKPF GRLWWDEIVP TVVTRAEPHN
760 770 780 790 800
QVIIHPEQNR VLSIRENARL QGFPDDYKLF GPPKQKYIQV GNAVAVPVAK
810 820 830
ALGYALGTAF QGLAVGKDPL LTLPEGFAFM KPTLPSELA
Length:839
Mass (Da):94,905
Last modified:July 25, 2006 - v2
Checksum:iB1B4D5F7AE0A3AAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138A → V in AAK71870 (PubMed:11349138).Curated1
Sequence conflicti165K → N in AAK71870 (PubMed:11349138).Curated1
Sequence conflicti166K → E in AAK69756 (PubMed:11459824).Curated1
Sequence conflicti266 – 268HKK → QKE in AAK71870 (PubMed:11349138).Curated3
Sequence conflicti433E → G in AAK71870 (PubMed:11349138).Curated1
Sequence conflicti821 – 822LT → II in AAK71870 (PubMed:11349138).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013289 Genomic DNA Translation: AAG52543.1
CP002684 Genomic DNA Translation: AEE34973.1
AF383170 Genomic DNA Translation: AAK69756.1
AF364174 Genomic DNA Translation: AAK71870.1
PIRiG96719
RefSeqiNP_177135.1, NM_105645.4
UniGeneiAt.35376

Genome annotation databases

EnsemblPlantsiAT1G69770.1; AT1G69770.1; AT1G69770
GeneIDi843313
GrameneiAT1G69770.1; AT1G69770.1; AT1G69770
KEGGiath:AT1G69770

Similar proteinsi

Entry informationi

Entry nameiCMT3_ARATH
AccessioniPrimary (citable) accession number: Q94F88
Secondary accession number(s): Q94FN4, Q9C9L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: April 25, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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