Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent Clp protease proteolytic subunit 4, chloroplastic

Gene

CLPP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). Essential protein required for chloroplast development and integrity (PubMed:16705403, PubMed:17241447). Essential for Embryogenesis (PubMed:23548781).By similarity3 Publications

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei158NucleophileBy similarity1
Active sitei183By similarity1

GO - Molecular functioni

GO - Biological processi

  • chloroplast organization Source: TAIR
  • regulation of timing of transition from vegetative to reproductive phase Source: TAIR

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS14.010

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit 4, chloroplastic1 Publication (EC:3.4.21.92)
Alternative name(s):
Endopeptidase ClpP41 Publication
Short name:
nClpP4
nClpP3
Gene namesi
Name:CLPP41 Publication
Synonyms:NCLPP3, NCLPP4
Ordered Locus Names:At5g45390Imported
ORF Names:MFC19.5Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G45390
TAIRilocus:2163538 AT5G45390

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo lethal when homozygous.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 65ChloroplastCombined sourcesAdd BLAST65
ChainiPRO_000030897966 – 292ATP-dependent Clp protease proteolytic subunit 4, chloroplasticAdd BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66N-acetylserineCombined sources1

Post-translational modificationi

Ubiquitinated by CHIP.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PaxDbiQ94B60
PRIDEiQ94B60

PTM databases

iPTMnetiQ94B60

Expressioni

Tissue specificityi

Mostly expressed in leaves. Also detected in stems, and to a lower extent, in roots (at protein level).1 Publication

Inductioni

Repressed in darkness. Levels decrease in leaves during aging (at protein level). Slightly and transiently repressed by high light stress (at protein level).3 Publications

Gene expression databases

ExpressionAtlasiQ94B60 baseline and differential
GenevisibleiQ94B60 AT

Interactioni

Subunit structurei

Component of the chloroplastic Clp protease core complex which consist of at least 16 proteins: CLPP4 (3 copies), CLPP5 (3 copies), CLPR4 (2 copies), ClpP1 (1 copy), CLPP6 (1 copy), CLPR2 (1 copy), CLPT1 (1 copy), CLPT2 (1 copy) and 3 copies of CLPP3 and/or CLPR1 and/or CLPR3 (PubMed:11278690, PubMed:14593120, PubMed:16766689, PubMed:16980539). Interacts with CHIP (PubMed:17241447). The core complex is organized in two heptameric rings, one containing CLPP3,4,5,6 in a 1:2:3:1 ratio and the other CLPP1 and CLPR1,2,3,4 in a 3:1:1:1:1 ratio (PubMed:21712416).6 Publications

Protein-protein interaction databases

BioGridi19824, 7 interactors
IntActiQ94B60, 2 interactors
STRINGi3702.AT5G45390.1

Structurei

3D structure databases

ProteinModelPortaliQ94B60
SMRiQ94B60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0840 Eukaryota
COG0740 LUCA
HOGENOMiHOG000285833
KOiK01358
OMAiANKRNVI
OrthoDBiEOG09360JU9
PhylomeDBiQ94B60

Family and domain databases

CDDicd07017 S14_ClpP_2, 1 hit
HAMAPiMF_00444 ClpP, 1 hit
InterProiView protein in InterPro
IPR001907 ClpP
IPR029045 ClpP/crotonase-like_dom_sf
IPR023562 ClpP/TepA
IPR033135 ClpP_His_AS
PANTHERiPTHR10381 PTHR10381, 1 hit
PfamiView protein in Pfam
PF00574 CLP_protease, 1 hit
PRINTSiPR00127 CLPPROTEASEP
SUPFAMiSSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00382 CLP_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94B60-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGTLSLSSSL KPSLVSSRLN SSSSASSSSF PKPNNLYLKP TKLISPPLRT
60 70 80 90 100
TSPSPLRFAN ASIEMSQTQE SAIRGAESDV MGLLLRERIV FLGSSIDDFV
110 120 130 140 150
ADAIMSQLLL LDAKDPKKDI KLFINSPGGS LSATMAIYDV VQLVRADVST
160 170 180 190 200
IALGIAASTA SIILGAGTKG KRFAMPNTRI MIHQPLGGAS GQAIDVEIQA
210 220 230 240 250
KEVMHNKNNV TSIIAGCTSR SFEQVLKDID RDRYMSPIEA VEYGLIDGVI
260 270 280 290
DGDSIIPLEP VPDRVKPRVN YEEISKDPMK FLTPEIPDDE IY
Length:292
Mass (Da):31,498
Last modified:December 1, 2001 - v1
Checksum:i227B514CF1C1CE4C
GO

Sequence cautioni

The sequence BAA82068 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB09167 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA04393 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 3MGT → TRP in CAA04393 (Ref. 2) Curated3
Sequence conflicti57R → T in CAA04393 (Ref. 2) Curated1
Sequence conflicti171K → N in CAA04393 (Ref. 2) Curated1
Sequence conflicti193A → V in AAM65254 (Ref. 6) Curated1
Sequence conflicti279M → I in AAM65254 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022329 mRNA Translation: BAA82068.1 Different initiation.
AJ000930 mRNA Translation: CAA04393.1 Different initiation.
AB018113 Genomic DNA Translation: BAB09167.1 Sequence problems.
CP002688 Genomic DNA Translation: AED95243.1
AY042832 mRNA Translation: AAK68772.1
BT006321 mRNA Translation: AAP13429.1
AY087717 mRNA Translation: AAM65254.1
PIRiT52452
RefSeqiNP_568644.1, NM_123907.4
UniGeneiAt.387
At.67124

Genome annotation databases

EnsemblPlantsiAT5G45390.1; AT5G45390.1; AT5G45390
GeneIDi834575
GrameneiAT5G45390.1; AT5G45390.1; AT5G45390
KEGGiath:AT5G45390

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022329 mRNA Translation: BAA82068.1 Different initiation.
AJ000930 mRNA Translation: CAA04393.1 Different initiation.
AB018113 Genomic DNA Translation: BAB09167.1 Sequence problems.
CP002688 Genomic DNA Translation: AED95243.1
AY042832 mRNA Translation: AAK68772.1
BT006321 mRNA Translation: AAP13429.1
AY087717 mRNA Translation: AAM65254.1
PIRiT52452
RefSeqiNP_568644.1, NM_123907.4
UniGeneiAt.387
At.67124

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R91model-A1-292[»]
ProteinModelPortaliQ94B60
SMRiQ94B60
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19824, 7 interactors
IntActiQ94B60, 2 interactors
STRINGi3702.AT5G45390.1

Protein family/group databases

MEROPSiS14.010

PTM databases

iPTMnetiQ94B60

Proteomic databases

PaxDbiQ94B60
PRIDEiQ94B60

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G45390.1; AT5G45390.1; AT5G45390
GeneIDi834575
GrameneiAT5G45390.1; AT5G45390.1; AT5G45390
KEGGiath:AT5G45390

Organism-specific databases

AraportiAT5G45390
TAIRilocus:2163538 AT5G45390

Phylogenomic databases

eggNOGiKOG0840 Eukaryota
COG0740 LUCA
HOGENOMiHOG000285833
KOiK01358
OMAiANKRNVI
OrthoDBiEOG09360JU9
PhylomeDBiQ94B60

Miscellaneous databases

PROiPR:Q94B60

Gene expression databases

ExpressionAtlasiQ94B60 baseline and differential
GenevisibleiQ94B60 AT

Family and domain databases

CDDicd07017 S14_ClpP_2, 1 hit
HAMAPiMF_00444 ClpP, 1 hit
InterProiView protein in InterPro
IPR001907 ClpP
IPR029045 ClpP/crotonase-like_dom_sf
IPR023562 ClpP/TepA
IPR033135 ClpP_His_AS
PANTHERiPTHR10381 PTHR10381, 1 hit
PfamiView protein in Pfam
PF00574 CLP_protease, 1 hit
PRINTSiPR00127 CLPPROTEASEP
SUPFAMiSSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00382 CLP_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLPP4_ARATH
AccessioniPrimary (citable) accession number: Q94B60
Secondary accession number(s): Q8LAN0
, Q9FHJ7, Q9SXJ5, Q9ZS78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again