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Protein

Cullin-1

Gene

CUL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Regulator of mitotic processes which plays a role during gametogenesis and embryogenesis. Together with SKP1, RBX1 and a F-box protein, it forms a SCF complex. The functional specificity of this complex depends of the type of F-box protein. SCF(UFO) is implicated in floral organ development. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1/ZTL), SCF(ADO2/LKP2), SCF(ADO3/FKF1) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • cell cycle Source: UniProtKB-KW
  • embryo development ending in seed dormancy Source: TAIR
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • jasmonic acid mediated signaling pathway Source: TAIR
  • leaf development Source: TAIR
  • phloem or xylem histogenesis Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of circadian rhythm Source: TAIR
  • response to auxin Source: TAIR
  • response to jasmonic acid Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionDevelopmental protein
Biological processAuxin signaling pathway, Cell cycle, Ethylene signaling pathway, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-1
Gene namesi
Name:CUL1
Ordered Locus Names:At4g02570
ORF Names:T10P11.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G02570
TAIRilocus:2132377 AT4G02570

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi682K → M: No neddylation. 2 Publications1
Mutagenesisi712K → M: No change in neddylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198041 – 738Cullin-1Add BLAST738

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki682Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)1 Publication

Post-translational modificationi

Neddylated (rubylated). Deneddylation occurs upon interaction with the COP9 signalosome (CSN) complex.2 Publications

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ94AH6
PRIDEiQ94AH6

Expressioni

Tissue specificityi

Expressed constitutively in roots, seedlings, stems, leaves and flowers.1 Publication

Developmental stagei

Strong accumulation in embryos (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ94AH6 baseline and differential
GenevisibleiQ94AH6 AT

Interactioni

Subunit structurei

Part of E3 ubiquitin ligase SCF complexes such as SCF(TIR1) and SCF(COI1). SCF(TIR1) is composed of CUL1, SKP1 (SKP1A or SKP1B), RBX1 (RBX1A or RBX1B) and TIR1, while SCF(COI1) is composed of CUL1, SKP1, RBX1 and COI1. A SNF1-related protein kinase (KIN10 and KIN11) can also be part of these SCF complexes. SCF(TIR1) is able to interact with the COP9 signalosome (CSN) complex. Interacts directly with some F-box proteins such as DOR, SKIP14, FBW2/SKIP18, SKIP19/FBL20 and SKIP28/MEE11. Interacts with CAND1.7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi10962, 47 interactors
DIPiDIP-33346N
IntActiQ94AH6, 37 interactors
STRINGi3702.AT4G02570.1

Structurei

3D structure databases

ProteinModelPortaliQ94AH6
SMRiQ94AH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2166 Eukaryota
COG5647 LUCA
HOGENOMiHOG000176712
InParanoidiQ94AH6
KOiK03347
OMAiRENDEGR
OrthoDBiEOG093603S8
PhylomeDBiQ94AH6

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q94AH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERKTIDLEQ GWDYMQTGIT KLKRILEGLN EPAFDSEQYM MLYTTIYNMC
60 70 80 90 100
TQKPPHDYSQ QLYDKYREAF EEYINSTVLP ALREKHDEFM LRELFKRWSN
110 120 130 140 150
HKVMVRWLSR FFYYLDRYFI ARRSLPPLNE VGLTCFRDLV YNELHSKVKQ
160 170 180 190 200
AVIALVDKER EGEQIDRALL KNVLDIYVEI GMGQMERYEE DFESFMLQDT
210 220 230 240 250
SSYYSRKASS WIQEDSCPDY MLKSEECLKK ERERVAHYLH SSSEPKLVEK
260 270 280 290 300
VQHELLVVFA SQLLEKEHSG CRALLRDDKV DDLSRMYRLY HKILRGLEPV
310 320 330 340 350
ANIFKQHVTA EGNALVQQAE DTATNQVANT ASVQEQVLIR KVIELHDKYM
360 370 380 390 400
VYVTECFQNH TLFHKALKEA FEIFCNKTVA GSSSAELLAT FCDNILKKGG
410 420 430 440 450
SEKLSDEAIE DTLEKVVKLL AYISDKDLFA EFYRKKLARR LLFDRSANDD
460 470 480 490 500
HERSILTKLK QQCGGQFTSK MEGMVTDLTL ARENQNSFED YLGSNPAANP
510 520 530 540 550
GIDLTVTVLT TGFWPSYKSF DINLPSEMIK CVEVFKGFYE TKTKHRKLTW
560 570 580 590 600
IYSLGTCHIN GKFDQKAIEL IVSTYQAAVL LLFNTTDKLS YTEILAQLNL
610 620 630 640 650
SHEDLVRLLH SLSCAKYKIL LKEPNTKTVS QNDAFEFNSK FTDRMRRIKI
660 670 680 690 700
PLPPVDERKK VVEDVDKDRR YAIDAAIVRI MKSRKVLGHQ QLVSECVEQL
710 720 730
SRMFKPDIKA IKKRMEDLIT RDYLERDKEN PNMFRYLA
Length:738
Mass (Da):86,302
Last modified:December 1, 2001 - v1
Checksum:i45041357DE8DAD8C
GO

Sequence cautioni

The sequence AAC78267 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80750 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318017 mRNA Translation: CAC85264.1
AC002330 Genomic DNA Translation: AAC78267.1 Sequence problems.
AL161494 Genomic DNA Translation: CAB80750.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE82194.1
CP002687 Genomic DNA Translation: AEE82195.1
CP002687 Genomic DNA Translation: AEE82196.1
CP002687 Genomic DNA Translation: AEE82197.1
AY046030 mRNA Translation: AAK76704.1
AY133878 mRNA Translation: AAM91812.1
AK222216 mRNA Translation: BAD95380.1
PIRiT01092
RefSeqiNP_001031575.1, NM_001036498.3
NP_001031576.1, NM_001036499.1
NP_001190661.1, NM_001203732.1
NP_567243.1, NM_116491.3
UniGeneiAt.24877
At.67849

Genome annotation databases

EnsemblPlantsiAT4G02570.1; AT4G02570.1; AT4G02570
AT4G02570.2; AT4G02570.2; AT4G02570
AT4G02570.3; AT4G02570.3; AT4G02570
AT4G02570.4; AT4G02570.4; AT4G02570
GeneIDi825648
GrameneiAT4G02570.1; AT4G02570.1; AT4G02570
AT4G02570.2; AT4G02570.2; AT4G02570
AT4G02570.3; AT4G02570.3; AT4G02570
AT4G02570.4; AT4G02570.4; AT4G02570
KEGGiath:AT4G02570

Similar proteinsi

Entry informationi

Entry nameiCUL1_ARATH
AccessioniPrimary (citable) accession number: Q94AH6
Secondary accession number(s): O22770, Q56W31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: July 18, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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