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UniProtKB - Q94AG6 (SAMC1_ARATH)
Protein
S-adenosylmethionine carrier 1, chloroplastic/mitochondrial
Gene
SAMC1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Transporter involved in exchange reactions through membranes. Has a low uniporter activity. Specifically mediates the transport of S-adenosylmethionine (SAM) and its closest analogs. Probably involved in the uptake of SAM in exchange for S-adenosylhomocysteine (SAHC), which is produced from SAM in the mitochondrial matrix and plastidial stroma by methyltransferase activities.
2 PublicationsActivity regulationi
Inhibited strongly by tannic acid, bromocresol purple, mercuric chloride, mersalyl, p-hydroxymercuribenzoate, S-adenosylhomocysteine, S-adenosylcysteine and adenosylornithine, and to a lesser extent by N-ethylmaleimide, bathophenanthroline and pyridoxal-5'-P.1 Publication
Kineticsi
- KM=95 µM for the SAM/SAM exchange1 Publication
- Vmax=1.2 mmol/min/g enzyme1 Publication
GO - Molecular functioni
GO - Biological processi
- chloroplast organization Source: TAIR
- S-adenosyl-L-methionine transport Source: TAIR
Keywordsi
Biological process | Transport |
Ligand | S-adenosyl-L-methionine |
Protein family/group databases
TCDBi | 2.A.29.18.2, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: S-adenosylmethionine carrier 1, chloroplastic/mitochondrialAlternative name(s): S-adenosylmethionine transporter 1 Short name: AtSAMT1 |
Gene namesi | Name:SAMC1 Synonyms:SAMT1 Ordered Locus Names:At4g39460 ORF Names:F23K16.90 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G39460 |
TAIRi | locus:2122452, AT4G39460 |
Subcellular locationi
Chloroplast
- chloroplast Source: TAIR
- chloroplast envelope Source: TAIR
- chloroplast membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial inner membrane Source: GO_Central
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- plastid Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 97 – 117 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 132 – 152 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 230 – 250 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 285 – 305 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Chloroplast, Membrane, Mitochondrion, PlastidPathology & Biotechi
Disruption phenotypei
Severely growth-retarded phenotype and reduced chlorophyll and plastoquinone contents. Unable to germinate when homozygous.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 38 | Chloroplast and mitochondrion1 PublicationAdd BLAST | 38 | |
ChainiPRO_0000424771 | 39 – 325 | S-adenosylmethionine carrier 1, chloroplastic/mitochondrialAdd BLAST | 287 |
Proteomic databases
PaxDbi | Q94AG6 |
PRIDEi | Q94AG6 |
ProteomicsDBi | 232731 |
Expressioni
Tissue specificityi
Expressed in seedlings, cotyledons, leaves and flowers. Lower levels of expression in stems and roots. Not detected in senescent leaves, petals and pollen grains.2 Publications
Gene expression databases
ExpressionAtlasi | Q94AG6, baseline and differential |
Genevisiblei | Q94AG6, AT |
Interactioni
Protein-protein interaction databases
IntActi | Q94AG6, 1 interactor |
STRINGi | 3702.AT4G39460.2 |
Structurei
3D structure databases
AlphaFoldDBi | Q94AG6 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 52 – 124 | Solcar 1Add BLAST | 73 | |
Repeati | 133 – 215 | Solcar 2Add BLAST | 83 | |
Repeati | 228 – 310 | Solcar 3Add BLAST | 83 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0768, Eukaryota |
HOGENOMi | CLU_015166_3_0_1 |
InParanoidi | Q94AG6 |
OMAi | PRVGWIS |
OrthoDBi | 1538959at2759 |
PhylomeDBi | Q94AG6 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q94AG6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPLTLSVDV KSSSATSHDV SKRVMQSSQL KINKGFFASV NTQEDKPFDF
60 70 80 90 100
FRTLFEGFIA GGTAGVVVET ALYPIDTIKT RLQAARGGGK IVLKGLYSGL
110 120 130 140 150
AGNIAGVLPA SALFVGVYEP TKQKLLKTFP DHLSAVAHLT AGAIGGLAAS
160 170 180 190 200
LIRVPTEVVK QRMQTGQFTS APSAVRMIAS KEGFRGLYAG YRSFLLRDLP
210 220 230 240 250
FDAIQFCIYE QLCLGYKKAA RRELSDPENA LIGAFAGALT GAVTTPLDVI
260 270 280 290 300
KTRLMVQGSA KQYQGIVDCV QTIVREEGAP ALLKGIGPRV LWIGIGGSIF
310 320
FGVLESTKRT LAQRRPNTVK ETKEE
Sequence cautioni
The sequence CAB44681 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80609 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ627908 mRNA Translation: CAF29517.1 AM260490 mRNA Translation: CAJ91123.1 AL078620 Genomic DNA Translation: CAB44681.1 Sequence problems. AL161595 Genomic DNA Translation: CAB80609.1 Sequence problems. CP002687 Genomic DNA Translation: AEE87074.1 CP002687 Genomic DNA Translation: AEE87075.1 CP002687 Genomic DNA Translation: ANM66368.1 AY046052 mRNA Translation: AAK76726.1 AY079349 mRNA Translation: AAL85080.1 |
PIRi | T09362 |
RefSeqi | NP_001190968.1, NM_001204039.1 NP_001328265.1, NM_001342543.1 NP_568060.1, NM_120106.3 |
Genome annotation databases
EnsemblPlantsi | AT4G39460.1; AT4G39460.1; AT4G39460 AT4G39460.2; AT4G39460.2; AT4G39460 AT4G39460.3; AT4G39460.3; AT4G39460 |
GeneIDi | 830101 |
Gramenei | AT4G39460.1; AT4G39460.1; AT4G39460 AT4G39460.2; AT4G39460.2; AT4G39460 AT4G39460.3; AT4G39460.3; AT4G39460 |
KEGGi | ath:AT4G39460 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ627908 mRNA Translation: CAF29517.1 AM260490 mRNA Translation: CAJ91123.1 AL078620 Genomic DNA Translation: CAB44681.1 Sequence problems. AL161595 Genomic DNA Translation: CAB80609.1 Sequence problems. CP002687 Genomic DNA Translation: AEE87074.1 CP002687 Genomic DNA Translation: AEE87075.1 CP002687 Genomic DNA Translation: ANM66368.1 AY046052 mRNA Translation: AAK76726.1 AY079349 mRNA Translation: AAL85080.1 |
PIRi | T09362 |
RefSeqi | NP_001190968.1, NM_001204039.1 NP_001328265.1, NM_001342543.1 NP_568060.1, NM_120106.3 |
3D structure databases
AlphaFoldDBi | Q94AG6 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q94AG6, 1 interactor |
STRINGi | 3702.AT4G39460.2 |
Protein family/group databases
TCDBi | 2.A.29.18.2, the mitochondrial carrier (mc) family |
Proteomic databases
PaxDbi | Q94AG6 |
PRIDEi | Q94AG6 |
ProteomicsDBi | 232731 |
Genome annotation databases
EnsemblPlantsi | AT4G39460.1; AT4G39460.1; AT4G39460 AT4G39460.2; AT4G39460.2; AT4G39460 AT4G39460.3; AT4G39460.3; AT4G39460 |
GeneIDi | 830101 |
Gramenei | AT4G39460.1; AT4G39460.1; AT4G39460 AT4G39460.2; AT4G39460.2; AT4G39460 AT4G39460.3; AT4G39460.3; AT4G39460 |
KEGGi | ath:AT4G39460 |
Organism-specific databases
Araporti | AT4G39460 |
TAIRi | locus:2122452, AT4G39460 |
Phylogenomic databases
eggNOGi | KOG0768, Eukaryota |
HOGENOMi | CLU_015166_3_0_1 |
InParanoidi | Q94AG6 |
OMAi | PRVGWIS |
OrthoDBi | 1538959at2759 |
PhylomeDBi | Q94AG6 |
Miscellaneous databases
PROi | PR:Q94AG6 |
Gene expression databases
ExpressionAtlasi | Q94AG6, baseline and differential |
Genevisiblei | Q94AG6, AT |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SAMC1_ARATH | |
Accessioni | Q94AG6Primary (citable) accession number: Q94AG6 Secondary accession number(s): Q9SVB2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 11, 2013 |
Last sequence update: | December 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families