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Entry version 170 (23 Feb 2022)
Sequence version 2 (01 Jul 1997)
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Protein

E3 ubiquitin-protein ligase ariadne-1

Gene

ari-1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Atypical E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 Ubc10 (PubMed:10880484, PubMed:21900267, PubMed:29689197).

Controls the subcellular localization and morphology of muscle nuclei (myonuclei) by regulating the protein levels and distribution of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex (PubMed:29689197).

Functions by mediating the monoubiquitination of the LINC complex subunit koi leading to its subsequent proteasomal degradation (PubMed:29689197).

Appears to function, at least partially redundantly, with the RBR E3 ligase family member park in nuclear localization and morphology (PubMed:29689197).

Likely to function in metamorphosis by regulating the proteins levels of EcR isoform A (ECR-A) and its heterodimeric partner usp, via the ubiquitination and subsequent degradation of ECR-A (PubMed:21900267).

3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi133Zinc 1PROSITE-ProRule annotation1
Metal bindingi136Zinc 1PROSITE-ProRule annotation1
Metal bindingi150Zinc 2PROSITE-ProRule annotation1
Metal bindingi152Zinc 2; via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi155Zinc 1PROSITE-ProRule annotation1
Metal bindingi158Zinc 1PROSITE-ProRule annotation1
Metal bindingi178Zinc 2PROSITE-ProRule annotation1
Metal bindingi183Zinc 2PROSITE-ProRule annotation1
Metal bindingi223Zinc 3PROSITE-ProRule annotation1
Metal bindingi228Zinc 3PROSITE-ProRule annotation1
Metal bindingi244Zinc 3PROSITE-ProRule annotation1
Metal bindingi246Zinc 3PROSITE-ProRule annotation1
Metal bindingi251Zinc 4PROSITE-ProRule annotation1
Metal bindingi254Zinc 4PROSITE-ProRule annotation1
Metal bindingi259Zinc 4; via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi264Zinc 4PROSITE-ProRule annotation1
Metal bindingi291Zinc 5PROSITE-ProRule annotation1
Metal bindingi294Zinc 5PROSITE-ProRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei304PROSITE-ProRule annotation1
Metal bindingi309Zinc 5PROSITE-ProRule annotation1
Metal bindingi314Zinc 5PROSITE-ProRule annotation1
Metal bindingi319Zinc 6PROSITE-ProRule annotation1
Metal bindingi322Zinc 6PROSITE-ProRule annotation1
Metal bindingi329Zinc 6; via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi336Zinc 6PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri133 – 183RING-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri203 – 264IBR-typePROSITE-ProRule annotationAdd BLAST62
Zinc fingeri291 – 322RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST32

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-DME-1169408, ISG15 antiviral mechanism

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...
SignaLinki
Q94981

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
E3 ubiquitin-protein ligase ariadne-11 Publication (EC:2.3.2.312 Publications)
Alternative name(s):
Protein ariadne-11 Publication
RING-type E3 ubiquitin transferase ariadne-1Curated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ari-11 PublicationImported
Synonyms:ari, ari-1a1 Publication
ORF Names:CG5659Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome X

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0017418, ari-1

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
VectorBase:FBgn0017418

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Pharate pupae lethal. Larval muscles exhibit an increase in nuclear clustering.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi136C → Y in ari-1a; Loss of catalytic activity. Late pupal lethal and adult escapers have a reduced lifespan and thinner bristles. Nuclei in larval muscles (myonuclei) are displaced from the cell membrane, exhibit clustering and morphological defects, likely due to the mislocalization of the LINC complex. Reduced degradation of the LINC complex member koi. 1 Publication1
Mutagenesisi150C → Y in ari1-2; lethal phenotype and loss of interaction with Ubc10. 1 Publication1
Mutagenesisi187V → E in ari-1b; Loss of catalytic activity. Late pupal lethal and adult escapers have a reduced lifespan and thinner bristles. Nuclei in larval muscles (myonuclei) are displaced from the cell membrane, exhibit clustering and morphological defects, likely due to the mislocalization of the LINC complex; when associated with P-332. 1 Publication1
Mutagenesisi223C → S in ari-1d; late pupal lethal and adult escapers have a reduced lifespan and thinner bristles. Nuclei in larval muscles (myonuclei) are displaced from the cell membrane, exhibit clustering and morphological defects. 1 Publication1
Mutagenesisi304C → S: Loss of catalytic activity and impaired autoinhibition; when associated with A-377; A-378 and A-450. 1 Publication1
Mutagenesisi309C → Y in ari1-3; lethal phenotype and no loss of interaction with Ubc10. 1 Publication1
Mutagenesisi332S → P in ari-1b; Loss of catalytic activity. Late pupal lethal and adult escapers have a reduced lifespan and thinner bristles. Nuclei in larval muscles (myonuclei) are displaced from the cell membrane, exhibit clustering and morphological defects, likely due to the mislocalization of the LINC complex; when associated with E-187. 1 Publication1
Mutagenesisi377F → A: Impairs autoinhibition; when associated with A-378 and A-450. Loss of activity and impaired autoinhibition; when associated with A-378; A-450 and S-304. 1 Publication1
Mutagenesisi378E → A: Impairs autoinhibition; when associated with A-377 and A-450. Loss of activity and impaired autoinhibition; when associated with A-377; A-450 and S-304. 1 Publication1
Mutagenesisi450E → A: Impairs autoinhibition; when associated with A-377 and A-378. Loss of activity and impaired autoinhibition; when associated with A-377; A-378 and S-304. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000557541 – 503E3 ubiquitin-protein ligase ariadne-1Add BLAST503

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q94981

PRoteomics IDEntifications database

More...
PRIDEi
Q94981

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Widely expressed, with prominent levels in the nervous system and female gonads.2 Publications

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed in all tissues throughout development, with maximum levels reached during metamorphosis and maintained in the adult.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
FBgn0017418, Expressed in embryo and 34 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q94981, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q94981, DM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Can form homodimers (PubMed:10880484, PubMed:29689197).

Interacts (via RING-type 1 zinc finger) with Ubc10 (PubMed:10880484, PubMed:29689197).

Interacts with the LINC complex member koi (PubMed:29689197).

Interacts with park (PubMed:29689197).

Interacts with ari-2 (PubMed:29689197). Specifically interacts with isoform ECR-A of EcR (PubMed:21900267).

3 Publications

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
59116, 13 interactors

Database of interacting proteins

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DIPi
DIP-17812N

Protein interaction database and analysis system

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IntActi
Q94981, 1 interactor

STRING: functional protein association networks

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STRINGi
7227.FBpp0297199

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q94981

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 40DisorderedSequence analysisAdd BLAST40
Regioni129 – 340TRIAD supradomainPROSITE-ProRule annotationAdd BLAST212
Regioni133 – 201Important for interaction with Ubc101 PublicationAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili341 – 361Sequence analysisAdd BLAST21

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Members of the RBR family are atypical E3 ligases. They interact with E2 conjugating enzymes such as Ubc10 and function like HECT-type E3 enzymes: they bind E2s via the first RING-type zinc finger, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved active site Cys residue in the second RING-type zinc finger. The active site probably forms a thioester intermediate with ubiquitin taken from the active-site cysteine of the E2 before ultimately transferring it to a Lys residue on the substrate.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the RBR family. Ariadne subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 183RING-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri203 – 264IBR-typePROSITE-ProRule annotationAdd BLAST62
Zinc fingeri291 – 322RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1815, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00940000155744

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_009823_4_2_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q94981

Identification of Orthologs from Complete Genome Data

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OMAi
DVDFAME

Database of Orthologous Groups

More...
OrthoDBi
469819at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q94981

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.30.40.10, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR045840, Ariadne
IPR031127, E3_UB_ligase_RBR
IPR002867, IBR_dom
IPR044066, TRIAD_supradom
IPR018957, Znf_C3HC4_RING-type
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD

The PANTHER Classification System

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PANTHERi
PTHR11685, PTHR11685, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF19422, Ariadne, 1 hit
PF01485, IBR, 2 hits
PF00097, zf-C3HC4, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00647, IBR, 2 hits

PROSITE; a protein domain and family database

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PROSITEi
View protein in PROSITE
PS51873, TRIAD, 1 hit
PS50089, ZF_RING_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q94981-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSDNDNDFC DNVDSGNVSS GDDGDDDFGM EVDLPSSADR QMDQDDYQYK
60 70 80 90 100
VLTTDEIVQH QREIIDEANL LLKLPTPTTR ILLNHFKWDK EKLLEKYFDD
110 120 130 140 150
NTDEFFKCAH VINPFNATEA IKQKTSRSQC EECEICFSQL PPDSMAGLEC
160 170 180 190 200
GHRFCMPCWH EYLSTKIVAE GLGQTISCAA HGCDILVDDV TVANLVTDAR
210 220 230 240 250
VRVKYQQLIT NSFVECNQLL RWCPSVDCTY AVKVPYAEPR RVHCKCGHVF
260 270 280 290 300
CFACGENWHD PVKCRWLKKW IKKCDDDSET SNWIAANTKE CPRCSVTIEK
310 320 330 340 350
DGGCNHMVCK NQNCKNEFCW VCLGSWEPHG SSWYNCNRYD EDEAKTARDA
360 370 380 390 400
QEKLRSSLAR YLHYYNRYMN HMQSMKFENK LYASVKQKME EMQQHNMSWI
410 420 430 440 450
EVQFLKKAVD ILCQCRQTLM YTYVFAYYLK KNNQSMIFED NQKDLESATE
460 470 480 490 500
MLSEYLERDI TSENLADIKQ KVQDKYRYCE KRCSVLLKHV HEGYDKEWWE

YTE
Length:503
Mass (Da):58,932
Last modified:July 1, 1997 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0AECCE256CF5EC00
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A4V4Q7A4V4Q7_DROME
RBR-type E3 ubiquitin transferase
ari-1 AAS65398.1, ari, Ari-1, ari-1a, ARI1_DROME
503Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X98309 Genomic DNA Translation: CAA66953.1
X98310 mRNA Translation: CAA66954.1
AE014298 Genomic DNA Translation: AAN09462.1

NCBI Reference Sequences

More...
RefSeqi
NP_001245736.1, NM_001258807.3
NP_001259671.1, NM_001272742.2
NP_001259672.1, NM_001272743.1
NP_523399.1, NM_078675.4
NP_728145.1, NM_167610.4
NP_996500.1, NM_206777.2

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0089506; FBpp0088499; FBgn0017418
FBtr0089507; FBpp0088500; FBgn0017418
FBtr0089508; FBpp0089023; FBgn0017418
FBtr0306057; FBpp0297199; FBgn0017418
FBtr0332850; FBpp0305073; FBgn0017418
FBtr0332851; FBpp0305074; FBgn0017418

Database of genes from NCBI RefSeq genomes

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GeneIDi
32796

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
dme:Dmel_CG5659

UCSC genome browser

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UCSCi
CG5659-RA, d. melanogaster

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98309 Genomic DNA Translation: CAA66953.1
X98310 mRNA Translation: CAA66954.1
AE014298 Genomic DNA Translation: AAN09462.1
RefSeqiNP_001245736.1, NM_001258807.3
NP_001259671.1, NM_001272742.2
NP_001259672.1, NM_001272743.1
NP_523399.1, NM_078675.4
NP_728145.1, NM_167610.4
NP_996500.1, NM_206777.2

3D structure databases

SMRiQ94981
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi59116, 13 interactors
DIPiDIP-17812N
IntActiQ94981, 1 interactor
STRINGi7227.FBpp0297199

Proteomic databases

PaxDbiQ94981
PRIDEiQ94981

Genome annotation databases

EnsemblMetazoaiFBtr0089506; FBpp0088499; FBgn0017418
FBtr0089507; FBpp0088500; FBgn0017418
FBtr0089508; FBpp0089023; FBgn0017418
FBtr0306057; FBpp0297199; FBgn0017418
FBtr0332850; FBpp0305073; FBgn0017418
FBtr0332851; FBpp0305074; FBgn0017418
GeneIDi32796
KEGGidme:Dmel_CG5659
UCSCiCG5659-RA, d. melanogaster

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
32796
FlyBaseiFBgn0017418, ari-1
VEuPathDBiVectorBase:FBgn0017418

Phylogenomic databases

eggNOGiKOG1815, Eukaryota
GeneTreeiENSGT00940000155744
HOGENOMiCLU_009823_4_2_1
InParanoidiQ94981
OMAiDVDFAME
OrthoDBi469819at2759
PhylomeDBiQ94981

Enzyme and pathway databases

ReactomeiR-DME-1169408, ISG15 antiviral mechanism
SignaLinkiQ94981

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

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BioGRID-ORCSi
32796, 1 hit in 1 CRISPR screen

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
32796

Protein Ontology

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PROi
PR:Q94981

Gene expression databases

BgeeiFBgn0017418, Expressed in embryo and 34 other tissues
ExpressionAtlasiQ94981, baseline and differential
GenevisibleiQ94981, DM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR045840, Ariadne
IPR031127, E3_UB_ligase_RBR
IPR002867, IBR_dom
IPR044066, TRIAD_supradom
IPR018957, Znf_C3HC4_RING-type
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD
PANTHERiPTHR11685, PTHR11685, 1 hit
PfamiView protein in Pfam
PF19422, Ariadne, 1 hit
PF01485, IBR, 2 hits
PF00097, zf-C3HC4, 1 hit
SMARTiView protein in SMART
SM00647, IBR, 2 hits
PROSITEiView protein in PROSITE
PS51873, TRIAD, 1 hit
PS50089, ZF_RING_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiARI1_DROME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q94981
Secondary accession number(s): Q0KHQ9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 1, 1997
Last modified: February 23, 2022
This is version 170 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families
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